Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Köln
[2021]
|
| Schlagwörter: | |
| Links: | https://d-nb.info/1239615655/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033268924&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 178 Seiten Illustrationen, Diagramme 21 cm |
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| 245 | 1 | 0 | |a Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China |c vorgelegt von Anna Ronge |
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Datensatz im Suchindex
| _version_ | 1819345732626284544 |
|---|---|
| adam_text | TABLE
OF
CONTENT
LIST
OF
ABBREVIATIONS
.........................................................................................................
11
1
INTRODUCTION
...................................................................................................................
13
1.1
GIARDIA
SPP..................................................................................................................
14
1.1.1
HISTORICAL
FACTS,
CURRENT
TAXONOMY
AND
SPECIES
...................................................
14
1.1.2
GIARDIASIS
CLINIC
AND
THERAPY
............................................................................
15
1.1.3
GIARDIA
LIFE
CYCLE
..............................................................................................
16
1.2
CRYPTOSPORIDIUM
SPP
...................................................................................................
17
1.2.1
HISTORICAL
FACTS,
CURRENT
TAXONOMY
AND
SPECIES
...................................................
17
1.2.2
CRYPTOSPORIDIOSIS
CLINIC
AND
THERAPY
.................................................................
19
1.2.3
CRYPTOSPORIDIUM
LIFE
CYCLE
...............................................................................
21
1.2.4
NEW
LIFE
CYCLE
OF
CRYPTOSPORIDIUM
(CLASSIFIED
TO
THE
GREGARINES)
....................
22
1.3
WATERBORNE
TRANSMISSION
OF
GIARDIA
AND
CRYPTOSPORIDIUM
SPP.
AND
OUTBREAKS
.....
24
1.4
AIM
OF
THE
PRESENT
THESIS
............................................................................................
25
2
MATERIALS
AND
METHODS
..................................................................................................
26
2.1
ORIGIN
AND
PROCESSING
OF
SAMPLES
..............................................................................
26
2.2
ORIGIN
OF
POSITIVE
CONTROL
SAMPLES
.............................................................................
27
2.2.1
GIARDIA
SPP
..........................................................................................................
27
2.2.2
CRYPTOSPORIDIUM
SPP
...........................................................................................
28
2.3
SAMPLE
PURIFICATION
....................................................................................................
28
2.3.1
PURIFICATION
OF
GIARDIA
CYSTS
IN
POSITIVE
SAMPLES
BY
SUCROSE
............................
28
2.3.2
PURIFICATION
OF
CRYPTOSPORIDIUM
OOCYSTS
IN
POSITIVE
SAMPLES
BY
DIETHYL
ETHER.
29
2.3.3
PURIFICATION
OF
CRYPTOSPORIDIUM
AND
GIARDIA
(OO)CYSTS
BY
PERCOLL
...................
30
2.4
MICROSCOPY
.................................................................................................................
30
2.4.1
GIARDIA
SPP
..........................................................................................................
30
2.4.1.1
NATIVE
(WITHOUT
STAINING)
.................................................................................
31
2.4.1.2
LUGOL
S
IODINE
..................................................................................................
31
2.4.1.3
GIEMSA
STAIN
.....................................................................................................
31
2.4.2
CRYPTOSPORIDIUM
SPP........................................................................................
31
2.4.2.1
NATIVE
(WITHOUT
STAINING)
.................................................................................
32
2.4.2.2 MODIFIED
ZIEHL-NEELSEN
STAIN
..........................................................................
32
2.4.2.3
CONTRAST
MICROSCOPY
........................................................................................
32
2.4.2.4 GIEMSA
STAIN
.....................................................................................................
33
2.4.2.5
EOSIN
STAIN
........................................................................................................
33
7
2.5
IMMUNOFLUORESCENCE
TEST
(IFT)
................................................................................
34
2.6
COUNTING
OF
CRYPTOSPORIDIUM
AND
GIARDIA
(OO)CYSTS
BY
HAEMOCYTOMETER..............
34
2.7
DNA
EXTRACTION
..........................................................................................................
36
2.7.1
DNA
EXTRACTION
FROM
PURIFIED
POSITIVE
CONTROLS
OR
LIQUID
SAMPLES
...................
36
2.7.2
A
LIMITED
DILUTION
METHOD
FOR
POSITIVE
CONTROLS
.................................................
37
2.7.3
DNA
EXTRACTION
FROM
FAECAL
SAMPLES
.................................................................
38
2.8
NESTED
PCR
AND
ELECTROPHORESIS
................................................................................
39
2.8.1
NESTED
PCR
FOR
THE
DETECTION
OF
GIARDIA
SPP
.....................................................
41
2.8.2
NESTED
PCR
FOR
THE
DETECTION
OF
CRYPTOSPORIDIUM
SPP
......................................
46
2.9
SEQUENCING
TO
DEFINE
THE
GIARDIA
AND
CRYPTOSPORIDIUM
SPECIES
AFTER
PCR
............
52
2.10
LAMP
ASSAYS
...........................................................................................................
52
2.10.1
LAMP
FOR
THE
DETECTION
OF
GIARDIA
SPP
............................................................
53
2.10.2
LAMP
FOR
THE
DETECTION
OF
CRYPTOSPORIDIUM
SPP
.............................................
53
2.11.
IN
VITRO
CULTURE
OF
GIARDIA
......................................................................................
54
2.12
OVERVIEW
OF
THE
DIAGNOSTIC
METHODS
.......................................................................
55
2.12.1
DIAGNOSIS
OF
GIARDIA
INFECTIONS
.......................................................................
56
2.12.2
DIAGNOSIS
OF
CRYPTOSPORIDIUM
INFECTIONS
.........................................................
57
2.13
STATISTICAL
ANALYSIS
....................................................................................................
58
2.13.1
CRITERIA
FOR
INCLUSION
IN
THE
GROUP
OF
PATIENTS
WITH
LOW
IMMUNITY
...............
59
3
RESULTS
........................................................................................................................
60
3.1
MICROSCOPY
.................................................................................................................
60
3.1.1
DETECTION
OF
GIARDIA
APPLYING
LUGOL
S
IODINE
..................................................
60
3.1.2
THE
SIZES
AND
OCCURRENCE
OF
GIARDIA
CYSTS
AND
TROPHOZOITES
............................
61
3.1.3
DETECTION
OF
CRYPTOSPORIDIUM
APPLYING
MODIFIED
ZIEHL-NEELSEN
STAIN
.............
62
3.1.4
DETECTION
OF
CRYPTOSPORIDIUM
APPLYING
CONTRAST
MICROSCOPY
..........................
63
3.2
IMMUNOFLUORESCENCE
TEST
(IFT)
................................................................................
64
3.3
DNA
EXTRACTION
..........................................................................................................
65
3.4
NESTED
PCR
...............................................................................................................
65
3.4.1
NESTED
PCR
FOR
THE
DETECTION
OF
GIARDIA
SPP
.....................................................
67
3.4.2
SENSITIVITY
AND
SPECIFICITY
FOR
THE
DETECTION
OF
GIARDIA
SPP
...............................
68
3.4.3
NESTED
PCR
FOR
THE
DETECTION
OF
CRYPTOSPORIDIUM
SPP
.....................................
69
3.4.4
SENSITIVITY
AND
SPECIFICITY
FOR
THE
DETECTION
OF
CRYPTOSPORIDIUM
SPP
...............
71
3.5
OVERVIEW
OF
THE
RESULTS
............................................................................................
73
8
3.5.1
DETECTION
OF
GIARDIA
SPP
.....................................................................................
73
3.5.2
DETECTION
OF
CRYPTOSPORIDIUM
SPP
......................................................................
74
3.5.3
DOUBLE-INFECTIONS
................................................................................................
75
3.5.4
GIARDIA
IN
A
SPUTUM
SAMPLE
................................................................................
75
3.6
SEQUENCING
.................................................................................................................
76
3.6.1
SEQUENCING
OF
GIARDIA
PCR-POSITIVE
SAMPLES
...................................................
76
3.6.2
SEQUENCING
OF
CRYPTOSPORIDIUM
POSITIVE
CONTROLS
.............................................
78
3.7
LAMP
ASSAYS
..............................................................................................................
79
3.7.1
LAMP
FOR
THE
DETECTION
OF
GIARDIA
SPP
..............................................................
80
3.7.2
LAMP
FOR
THE
DETECTION
OF
CRYPTOSPORIDIUM
SPP
...............................................
81
3.8
IN
VITRO
CULTURE
OF
GIARDIA
SPP
....................................................................................
81
3.9
STATISTICAL
ANALYSIS
......................................................................................................
81
3.9.1
STATISTICAL
ANALYSIS:
GIARDIA
SPP
..........................................................................
82
3.9.2
STATISTICAL
ANALYSIS:
CRYPTOSPORIDIUM
SPP...........................................................
83
4
DISCUSSION
..................................................................................................................
85
4.1
THE
QINGHAI
PROVINCE
AS
PART
OF
THE
TIBETAN
PLATEAU
AND
ITS
PEOPLE
........................
85
4.2
GIARDIA
AND
CRYPTOSPORIDIUM
EPIDEMIOLOGY
AND
INFECTIONS
IN
CHINA
AND
QINGHAI
PROVINCE
...........................................................................................................................
87
4.3
DIAGNOSIS
OF
GIARDIASIS
AND
CRYPTOSPORIDIOSIS
-
THE
CHOICE
OF
SCREENING
METHOD....
89
4.3.1
SAMPLE
CHOICE
.....................................................................................................
89
4.3.2
PURIFICATION
PROTOCOLS
..........................................................................................
90
4.3.3
MICROSCOPIC
DETECTION
OF
GIARDIA
SPP.................................................................
91
4.3.4
MICROSCOPIC
DETECTION
OF
CRYPTOSPORIDIUM
SPP
..................................................
92
4.3.5
IFT
FOR
THE
DETECTION
OF
GIARDIA
AND
CRYPTOSPORIDIUM
SPP
...............................
94
4.3.6
NESTED
PCR
FOR
THE
DETECTION
OF
GIARDIA
AND
CRYPTOSPORIDIUM
SPP
..................
94
4.3.7
SEQUENCING
OF
GIARDIA
AND
CRYPTOSPORIDIUM
POSITIVE
N-PCR
RESULTS
...............
95
4.3.8
LAMP
FOR
THE
DETECTION
OF
BOTH
PARASITES
..........................................................
96
4.4
DISCUSSION
OF
THE
OWN
RESULTS
....................................................................................
97
4.4.1
DIAGNOSIS
OF
GIARDIAL
AND
CRYPTOSPORIDIAL
INFECTIONS..........................................
98
4.4.2
MIXED
(DOUBLE)-INFECTIONS
..................................................................................
99
4.4.3
GIARDIA
IN
A
SPUTUM
...........................................................................................
100
4.4.4
ATTEMPTS
FOR
THE
IN
VITRO
CULTURE
OF
GIARDIA
ISOLATES
.........................................
100
4.4.5
STATISTICAL
RESULTS
...............................................................................................
101
4.5
TRANSMISSION
ROUTES
OF
GIARDIA
AND
CRYPTOSPORIDIUM
IN
CHINA
AND
WORLDWIDE...
102
9
4.5.1
THE
WATERBORNE
TRANSMISSION
OF
GIARDIA
AND
CRYPTOSPORIDIUM
.....................
102
4.5.2
THE
FOODBOME
TRANSMISSION
OF
GIARDIA
AND
CRYPTOSPORIDIUM
.....................
103
4.5.3
THE
RESPIRATORY
TRANSMISSION
OF
GIARDIA
AND
CRYPTOSPORIDIUM.....................
105
4.7
CHINA
S
PEOPLE,
THEIR
CULTURE
AND
THEIR
HYGIENIC
BEHAVIOUR.....................................
109
4.8
MY
STAY,
THE
RESEARCH
PERIOD
AND
WORKING
CONDITIONS
IN
CHINA
..............................
ILL
4.9
THE
ONLINE
CITATIONS
FOR
THE
PRESENT
WORK
................................................................
113
4.10
CONCLUSION
AND
RECOMMENDATIONS
.........................................................................
113
5
SUMMARY
(ENGLISH)
.....................................................................................................
116
ZUSAMMENFASSUNG
(DEUTSCH)
.........................................................................................
117
6
REFERENCES
...................................................................................................................
118
7
APPENDIX
.....................................................................................................................
142
7.1
LIST
OF
FIGURES
...........................................................................................................
142
7.2
LIST
OF
TABLES
............................................................................................................
143
7.3
STATISTICAL
ANALYSIS:
TABLES
AND
FIGURES
....................................................................
143
7.3.1.
RESULTS:
GIARDIA
...............................................................................................
143
7.3.2
RESULTS:
CRYPTOSPORIDIUM
..............................................................................
145
7.4
SAMPLE
LIST
WITH
FINDINGS
.........................................................................................
148
7.5
ADDITIONAL
INFORMATION
.............................................................................................
175
8
LEBENSLAUF
...................................................................................................................
176
10
|
| any_adam_object | 1 |
| author | Ronge, Anna 1992- |
| author_GND | (DE-588)1237701333 |
| author_facet | Ronge, Anna 1992- |
| author_role | aut |
| author_sort | Ronge, Anna 1992- |
| author_variant | a r ar |
| building | Verbundindex |
| bvnumber | BV047886723 |
| ctrlnum | (OCoLC)1282182832 (DE-599)DNB1239615655 |
| discipline | Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047886723 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T19:31:21Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033268924 |
| oclc_num | 1282182832 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 178 Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| record_format | marc |
| spellingShingle | Ronge, Anna 1992- Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China |
| subject_GND | (DE-588)4113937-9 |
| title | Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China |
| title_auth | Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China |
| title_exact_search | Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China |
| title_full | Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China vorgelegt von Anna Ronge |
| title_fullStr | Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China vorgelegt von Anna Ronge |
| title_full_unstemmed | Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China vorgelegt von Anna Ronge |
| title_short | Gastrointestinale parasitäre Infektionen in Diarrhö-Patientinnen und Patienten des Universitätskrankenhauses in Xining, Qinghai Provinz, im Tibetischen Hochland in China |
| title_sort | gastrointestinale parasitare infektionen in diarrho patientinnen und patienten des universitatskrankenhauses in xining qinghai provinz im tibetischen hochland in china |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1239615655/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033268924&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT rongeanna gastrointestinaleparasitareinfektionenindiarrhopatientinnenundpatientendesuniversitatskrankenhausesinxiningqinghaiprovinzimtibetischenhochlandinchina |