Manual of sperm function testing in human assisted reproduction:
Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances...
Gespeichert in:
| Weitere beteiligte Personen: | , , |
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| Format: | Elektronisch E-Book |
| Sprache: | Englisch |
| Veröffentlicht: |
Cambridge
Cambridge University Press
2021
|
| Schlagwörter: | |
| Links: | https://doi.org/10.1017/9781108878715 https://doi.org/10.1017/9781108878715 https://doi.org/10.1017/9781108878715 |
| Zusammenfassung: | Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances of producing viable and healthy embryos. Focusing on modern sperm function testing, this manual provides technical details of commonly used tests and gives an overview of the laboratory techniques used to evaluate sperm samples. Covering a variety of testing methods in detail, from manual and computer-assisted semen analysis to zona pellucida binding assays, and tests assessing sperm DNA damage such as the TUNEL assay. Describing the underlying science, practical advice for performing the tests is given, including tips for optimizing outcomes and trouble-shooting. This is an essential guide for reproductive medicine specialists, clinical andrologists, urologists and gynecologists working with sub-fertile men |
| Beschreibung: | Title from publisher's bibliographic system (viewed on 09 Apr 2021) Basic Semen Examination -- Standard semen analysis : Computer-assisted semen analysis -- Standard semen analysis : home sperm testing -- Standard semen analysis : Leukocytospermia -- Standard semen analysis : Sperm Morphology Evaluation -- Sperm vitality : eosinnigrosin dye exclusion -- Sperm vitality : the hypoosmotic swelling test -- Determination of mitochondrial membrane potential by flow cytometry in human sperm cells -- Capacitation and acrosome reaction : fluorescence techniques to determine acrosome reaction -- Capacitation and acrosome reaction : Histochemical techniques to assess the acrosome reaction -- Zona binding : competitive sperm-binding assay -- Zona binding-Hemizona assay -- Oolemma Binding : Sperm Penetration Assay -- Oxidative Stress Testing : Direct Tests -- Oxidative stress in male Infertility : Indirect tests -- Chromatin condensation : Aniline blue staining -- Chromatin condensation : chromomycin A3 (CMA3) stain -- Chromatin Condensation : Toluidine Blue Staining -- TdT-mediated dUTP nickend-labelling (TUNEL) assay -- DNA damage : sperm chromatin structure assay (SCSA) -- Sperm DNA damage measured by the Comet assay -- Sperm Chromatin Dispersion test -- Fluorescent in-situ hybridization -- Clinical Value of Sperm Function Tests -- Sperm Proteomics |
| Umfang: | 1 Online-Ressource (xv, 270 Seiten) |
| ISBN: | 9781108878715 |
| DOI: | 10.1017/9781108878715 |
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| 520 | |a Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances of producing viable and healthy embryos. Focusing on modern sperm function testing, this manual provides technical details of commonly used tests and gives an overview of the laboratory techniques used to evaluate sperm samples. Covering a variety of testing methods in detail, from manual and computer-assisted semen analysis to zona pellucida binding assays, and tests assessing sperm DNA damage such as the TUNEL assay. Describing the underlying science, practical advice for performing the tests is given, including tips for optimizing outcomes and trouble-shooting. This is an essential guide for reproductive medicine specialists, clinical andrologists, urologists and gynecologists working with sub-fertile men | ||
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| illustrated | Not Illustrated |
| indexdate | 2024-12-20T19:16:46Z |
| institution | BVB |
| isbn | 9781108878715 |
| language | English |
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| spelling | Manual of sperm function testing in human assisted reproduction edited by Ashok Agarwal, Ralf Henkel, Ahmad Majzoub Cambridge Cambridge University Press 2021 1 Online-Ressource (xv, 270 Seiten) txt rdacontent c rdamedia cr rdacarrier Title from publisher's bibliographic system (viewed on 09 Apr 2021) Basic Semen Examination -- Standard semen analysis : Computer-assisted semen analysis -- Standard semen analysis : home sperm testing -- Standard semen analysis : Leukocytospermia -- Standard semen analysis : Sperm Morphology Evaluation -- Sperm vitality : eosinnigrosin dye exclusion -- Sperm vitality : the hypoosmotic swelling test -- Determination of mitochondrial membrane potential by flow cytometry in human sperm cells -- Capacitation and acrosome reaction : fluorescence techniques to determine acrosome reaction -- Capacitation and acrosome reaction : Histochemical techniques to assess the acrosome reaction -- Zona binding : competitive sperm-binding assay -- Zona binding-Hemizona assay -- Oolemma Binding : Sperm Penetration Assay -- Oxidative Stress Testing : Direct Tests -- Oxidative stress in male Infertility : Indirect tests -- Chromatin condensation : Aniline blue staining -- Chromatin condensation : chromomycin A3 (CMA3) stain -- Chromatin Condensation : Toluidine Blue Staining -- TdT-mediated dUTP nickend-labelling (TUNEL) assay -- DNA damage : sperm chromatin structure assay (SCSA) -- Sperm DNA damage measured by the Comet assay -- Sperm Chromatin Dispersion test -- Fluorescent in-situ hybridization -- Clinical Value of Sperm Function Tests -- Sperm Proteomics Selecting good-quality sperm for use in in-vitro fertilization is a key step in assisted reproduction. For many years purely morphological attributes have been used to assess suitability, but increasingly biochemical and molecular biological techniques are now identifying sperm with the best chances of producing viable and healthy embryos. Focusing on modern sperm function testing, this manual provides technical details of commonly used tests and gives an overview of the laboratory techniques used to evaluate sperm samples. Covering a variety of testing methods in detail, from manual and computer-assisted semen analysis to zona pellucida binding assays, and tests assessing sperm DNA damage such as the TUNEL assay. Describing the underlying science, practical advice for performing the tests is given, including tips for optimizing outcomes and trouble-shooting. This is an essential guide for reproductive medicine specialists, clinical andrologists, urologists and gynecologists working with sub-fertile men Spermatozoa / Physiology Infertility, Male Agarwal, Ashok (DE-588)1148694706 edt Henkel, Ralf 1960- (DE-588)1235882985 edt Majzoub, Ahmad (DE-588)1148694013 edt Erscheint auch als Druck-Ausgabe 978-1-108-79353-7 https://doi.org/10.1017/9781108878715 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Manual of sperm function testing in human assisted reproduction Spermatozoa / Physiology Infertility, Male |
| title | Manual of sperm function testing in human assisted reproduction |
| title_auth | Manual of sperm function testing in human assisted reproduction |
| title_exact_search | Manual of sperm function testing in human assisted reproduction |
| title_full | Manual of sperm function testing in human assisted reproduction edited by Ashok Agarwal, Ralf Henkel, Ahmad Majzoub |
| title_fullStr | Manual of sperm function testing in human assisted reproduction edited by Ashok Agarwal, Ralf Henkel, Ahmad Majzoub |
| title_full_unstemmed | Manual of sperm function testing in human assisted reproduction edited by Ashok Agarwal, Ralf Henkel, Ahmad Majzoub |
| title_short | Manual of sperm function testing in human assisted reproduction |
| title_sort | manual of sperm function testing in human assisted reproduction |
| topic | Spermatozoa / Physiology Infertility, Male |
| topic_facet | Spermatozoa / Physiology Infertility, Male |
| url | https://doi.org/10.1017/9781108878715 |
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