Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Ulm
2019
|
| Schlagwörter: | |
| Links: | https://d-nb.info/1196982872/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031649285&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | VII, 164 Blätter Illustrationen, Diagramme 30 cm |
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| 245 | 1 | 0 | |a Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies |c Johanna Blaha |
| 264 | 1 | |a Ulm |c 2019 | |
| 300 | |a VII, 164 Blätter |b Illustrationen, Diagramme |c 30 cm | ||
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| 502 | |b Dissertation |c Universität Ulm |d 2019 | ||
| 653 | |a Induced pluripotent stem cells | ||
| 653 | |a Immunologic deficiency syndromes | ||
| 653 | |a Genetics | ||
| 653 | |a Sequence analysis, DNA | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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Datensatz im Suchindex
| DE-BY-TUM_call_number | 0001 2023 B 504 |
|---|---|
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| adam_text | TABLE
OF
CONTENT
LIST
OF
ABBREVIATIONS
.................................................................................................................
IV
1.
INTRODUCTION
......................................................................................................................
-1
-
1.1.
HUMAN
INDUCED
PLURIPOTENT
STEM
CELLS
...................................................................
-1
-
1.2.
CRISPR/CAS9
.............................................................................................................
-4
-
1.3.
PRIMARY
IMMUNODEFICIENCY
DISEASE
........................................................................
-
9
-
1.4.
AIM
OF
THE
STUDY
......................................................................................................
-18
-
2.
MATERIAL
AND
METHODS
....................................................................................................
-19-
2.1.
CELL
CULTURE
...............................................................................................................
-19-
2.2.
MOLECULAR
BIOLOGY
METHODS
..................................................................................
-
28
-
2.3.
PROTEIN
BIOCHEMISTRY
..............................................................................................
-
37
-
2.4.
STATISTICAL
ANALYSIS
..................................................................................................
-
42
-
2.5.
MATERIALS
.................................................................................................................
-
42
-
3.
RESULTS.............................................................................................................................
-45
-
3.1.
WILD
TYPE
IPSC
ESTABLISHMENT
...............................................................................
-45
-
3.2.
VALIDATION
AND
SELECTION
OF
CANDIDATE
GENES
......................................................
-
50
-
3.3.
INTRODUCTION
OF
CANDIDATE
GENE
VARIANTS
INTO
IPSCS
............................................
-
71
-
3.4.
VALIDATION
AND
SELECTION
OF
IPSC
CLONES
FOR
FUTURE
EXPERIMENTS
.......................
-
84
-
3.5.
QUALITY
CONTROL
OF
VARIANT
IPSC
CLONES
..................................................................
-
89
-
3.6.
CHARACTERIZATION
OF
VARIANT
IPSC
CLONES
...................... .......................................
-
95
-
3.7.
DIFFERENTIATION
OF
IPSCS
TOWARDS
HEMATOPOIETIC
STEM
CELLS
...........................
-100
-
3.8.
DIFFERENTIATION
OF
IPSCS
TOWARDS
NK
CELLS
.........................................................
-105
-
3.9.
DIFFERENTIATION
OF
IPSCS
TOWARDS
B
CELLS
............................................................
-112
-
4.
DISCUSSION
....................................................................................................................
-116
-
4.1.
IPSCS
.....................................................................................................................
-116
-
4.2.
TRANSFECTION
AND
CRISPR/CAS9
STRATEGY
.............................................................
-117
-
4.3. CLONE
SELECTION
.....................................................................................................
-118-
4.4.
DIFFERENTIATION
EXPERIMENTS
...............................................................................
-119
-
4.5.
DISEASES
WITH
SIMILAR
PHENOTYPES
......................................................................
-125
-
II
4.6.
CANDIDATE
GENES
..................................................................................................
-126
-
4.7.
GENERAL
COMMENTS
ON
HIGH-THROUGHPUT
SEQUENCING
.......................................
-134
-
4.8.
CONCLUSION
............................................................................................................
-135
-
5.
SUMMARY
.......................................................................................................................
-136
-
6.
LIST
OF
REFERENCES
........................................................................................................
-138
-
7.
APPENDIX
.......................................................................................................................
-161
-
III
|
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| bvnumber | BV046271484 |
| ctrlnum | (OCoLC)1129402063 (DE-599)KXP1677699434 |
| discipline | Biologie Medizin |
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| id | DE-604.BV046271484 |
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| indexdate | 2024-12-20T18:47:52Z |
| institution | BVB |
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| language | English |
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| physical | VII, 164 Blätter Illustrationen, Diagramme 30 cm |
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| spellingShingle | Blaha, Johanna Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies |
| subject_GND | (DE-588)4113937-9 |
| title | Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies |
| title_auth | Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies |
| title_exact_search | Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies |
| title_full | Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies Johanna Blaha |
| title_fullStr | Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies Johanna Blaha |
| title_full_unstemmed | Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies Johanna Blaha |
| title_short | Establishment of an in vitro system using human induced pluripotent stem cells and CRISPR/Cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies |
| title_sort | establishment of an in vitro system using human induced pluripotent stem cells and crispr cas9 for the imitation of genetic defects exemplified by primary immunodeficiencies |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1196982872/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031649285&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT blahajohanna establishmentofaninvitrosystemusinghumaninducedpluripotentstemcellsandcrisprcas9fortheimitationofgeneticdefectsexemplifiedbyprimaryimmunodeficiencies AT universitatulm establishmentofaninvitrosystemusinghumaninducedpluripotentstemcellsandcrisprcas9fortheimitationofgeneticdefectsexemplifiedbyprimaryimmunodeficiencies |
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