Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression: = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Erlangen ; Nürnberg
[2019]
|
| Schlagwörter: | |
| Links: | http://d-nb.info/1182058434/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030936556&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 96 Seiten Illustrationen, Diagramme 21 cm |
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| 245 | 1 | 0 | |a Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression |b = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression |c vorgelegt von Danyil Huraskin |
| 246 | 1 | 3 | |a Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression |
| 246 | 1 | 1 | |a Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression |
| 264 | 1 | |a Erlangen ; Nürnberg |c [2019] | |
| 300 | |a 96 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
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| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2019 | ||
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Datensatz im Suchindex
| _version_ | 1819330477011501056 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
ZUSAMMENFASSUNG
.........................................................................................................................................
5
SUMMARY
........................................................................................................................................................
7
1.
INTRODUCTION
...........................................................................................................................................
8
1.1.
WNT
SIGNALING
PATHWAYS
-
REGULATORS
OF
DEVELOPMENTAL
PROCESSES
AND
TISSUE
SPECIFIC
PROLIFERATION
AND
DIFFERENTIATION
OF
CELLS
..................................................................................................
8
1.2.
TCF/LEF
AND
TLE
TRANSCRIPTIONAL
REGULATORS
................................................................................
9
1.3.
THE
REGULATION
OF
BETA-CATENIN
SIGNALING
BY
AXIN-1
AND
AXIN-2
.................................................
9
1.4.
HIPPO
SIGNALING
PATHWAY
AND
YAP/TAZ/TEAD
ACTIVITY
-
REGULATION
OF
ORGAN
SIZE,
STEM
CELL
SELF-RENEWAL
AND
REGENERATION
...............................................................................................................
10
1.5.
CROSSTALK
BETWEEN
WNT
AND
YAP/TAZ/TEAD
SIGNALING
PATHWAYS
............................................
12
1.6.
THE
SKELETAL
MUSCLE
ARCHITECTURE
AND
MUSCLE
FIBER
TYPES
........................................................
13
1.7.
THE
SKELETAL
MUSCLE
CELL
LINEAGE
.................................................................................................
15
1.8.
DEVELOPMENTAL
MYOGENESIS
.......................................................................................................
16
1.9.
ADULT
MYOGENESIS
AND
MUSCLE
SATELLITE
CELLS
.............................................................................
16
1.10.
STRUCTURE,
FUNCTION,
AND
DEVELOPMENT
OF
THE
NEUROMUSCULAR
JUNCTION
.................................
17
1.11.
WNT
AND
YAP/TAZ/TEAD
SIGNALING
THROUGHOUT
THE
MUSCLE
CELL
LINEAGE
.................................
19
1.12.
WNT
AND
YAP/TAZ/TEAD
SIGNALING
IN
REGULATION
OF
SKELETAL
MUSCLE
FIBER
TYPE
AND
SIZE
.......
22
1.13.
THE
NEUROMUSCULAR
ROLE
OF
WNT
AND
YAP/TAZ/TEAD
SIGNALING
PATHWAYS
..............................
23
1.14.
PRELIMINARY
WORK
........................................................................................................................
25
2.
RESULTS
..................................................................................................................................................
29
2.1.
IN
DEPTH
ANALYSIS
OF
CANONICAL
WNT/BETA-CATENIN
SIGNALING
ACTIVITY
AND
CORRELATION
WITH
YAP/TAZ/TEAD
SIGNALING
IN
SKELETAL
MUSCLE
..........................................................................................
29
2.2.
BETA-CATENIN
AND
YAP/TAZ/TEAD
SIGNALING
ARE
ACTIVE
IN
MYOGENESIS
.....................................
35
2.3.
CANONICAL
WNTS
REGULATE
CANONICAL
WNT/BETA-CATENIN
AND
YAP/TAZ/TEAD
SIGNALING
ACTIVITY
IN
MUSCLE
CELLS
.........................................................................................................................................
42
2.4.
FEEDBACK
INHIBITION
OF
CANONICAL
WNT/BETA-CATENIN
SIGNALING
BY
AXIN2
AND
YAP/TAZ/TEAD
SIGNALING
REGULATE
MYOGENESIS
...............................................................................................................
45
2.5.
THE
NEUROMUSCULAR
ROLE
OF
CANONICAL
WNT/BETA-CATENIN
AND
YAP/TAZ/TEAD
SIGNALING
.......48
3.
DISCUSSION
.........................................................................................................
56
4.
METHODS
AND
MATERIALS
......................................................................................................................
64
4.1.
METHODS
...........................................................................................................................................
64
4.1.1
PLASMIDS
AND
CLONING
..............................................................................................................
64
4.1.2
TRANSFORMATION,
PLASMID
PREPARATION
...................................................................................
64
4.1.3
IN
SITU
HYBRIDIZATIONS
..............................................................................................................
65
4.1.4
RNA
EXTRACTION,
REVERSE
TRANSCRIPTION,
QPCR
.......................................................................
65
4.1.5
TISSUE
CULTURE
.........................................................................................................................
66
3
4.1.6
SATELLITE
CELL
ISOLATION
..............................................................................................................
66
4.1.7
CELL
TRANSFECTION
.......................................................................................................................
67
4.1.8
GENERATION
OF
KNOCKOUT
PRIMARY
SKELETAL
MUSCLE
CELLS
..........................................................
67
4.1.9
ISOLATION
AND
CULTURE
OF
EDI
SINGLE
FIBER
EXPLANTS
................................................................
68
4.1.10
PROTEIN
LYSATES,
SDS-PAGE,
WESTERN
BLOT
..........................................................................
68
4.1.11
TISSUE
SECTIONS
AND
HISTOCHEMISTRY
...................................................................................
69
4.1.12
IMMUNOFLUORESCENCE
STAINING
AND
MICROSCOPY
................................................................
69
4.1.13
LUCIFERASE-ASSAYS
................................................................................................................
70
4.1.14
MOUSE
PROCEDURES
AND
GENOTYPING
...................................................................................
71
4.1.15
IN
SILICO
ANALYSIS
....................................................................................................................
72
4.2.
MATERIALS
............................................................................................................................................
73
4.2.1
OLIGONUCLEOTIDES
......................................................................................................................
73
4.2.2
PLASMIDS
..............................................................................................
75
4.2.3
ANTIBODIES
AND
PROTEINS
USED
FOR
IMMUNOHISTOCHEMISTRY
..................................................
75
4.2.4
BUFFERS
AND
SOLUTIONS
..............................................................................................................
76
5
LIST
OF
ABBREVIATIONS
............................................................................................................................
79
6
LITERATURE
..............................................................................................................................................
82
7
ACKNOWLEDGEMENTS
.............................................................................................................................
95
8
PUBLICATIONS
.................................
96
|
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| author | Huraskin, Danyil |
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| ctrlnum | (OCoLC)1121486945 (DE-599)DNB1182058434 |
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| id | DE-604.BV045552614 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T18:30:10Z |
| institution | BVB |
| language | English |
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| spellingShingle | Huraskin, Danyil Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression Signaltransduktion (DE-588)4318717-1 gnd Acetylcholinrezeptor (DE-588)4133006-7 gnd Muskelentwicklung (DE-588)4170853-2 gnd |
| subject_GND | (DE-588)4318717-1 (DE-588)4133006-7 (DE-588)4170853-2 (DE-588)4113937-9 |
| title | Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression |
| title_alt | Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression |
| title_auth | Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression |
| title_exact_search | Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression |
| title_full | Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression vorgelegt von Danyil Huraskin |
| title_fullStr | Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression vorgelegt von Danyil Huraskin |
| title_full_unstemmed | Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression vorgelegt von Danyil Huraskin |
| title_short | Expression profiling of canonical Wnt/beta-catenin and YAP/TAZ/TEAD signaling members and their regulatory role in myogenesis, acetylcholine receptor clustering and synaptic gene expression |
| title_sort | expression profiling of canonical wnt beta catenin and yap taz tead signaling members and their regulatory role in myogenesis acetylcholine receptor clustering and synaptic gene expression expressionsprofil und regulation von effektoren der kanonischen wnt beta catenin und yap taz tead signaltransduktion bei der myogenese der aggregation von acetylcholin rezeptoren und synaptischer genexpression |
| title_sub | = Expressionsprofil und Regulation von Effektoren der kanonischen Wnt/beta-Catenin und YAP/TAZ/TEAD Signaltransduktion bei der Myogenese, der Aggregation von Acetylcholin-Rezeptoren und synaptischer Genexpression |
| topic | Signaltransduktion (DE-588)4318717-1 gnd Acetylcholinrezeptor (DE-588)4133006-7 gnd Muskelentwicklung (DE-588)4170853-2 gnd |
| topic_facet | Signaltransduktion Acetylcholinrezeptor Muskelentwicklung Hochschulschrift |
| url | http://d-nb.info/1182058434/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030936556&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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