Axonal degeneration in Parkinson's disease: = Axonale Degeneration beim Parkinson Syndrom
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Erlangen ; Nürnberg
[2019]
|
| Schlagwörter: | |
| Links: | http://d-nb.info/1182063799/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030936339&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 77, VII Seiten Illustrationen, Diagramme 21 cm |
Internformat
MARC
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| 100 | 1 | |a Grosch, Janina |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Axonal degeneration in Parkinson's disease |b = Axonale Degeneration beim Parkinson Syndrom |c vorgelegt von Janina Grosch (geb. Deußer) |
| 246 | 1 | 3 | |a Axonale Degeneration beim Parkinson Syndrom |
| 246 | 1 | 1 | |a Axonale Degeneration beim Parkinson Syndrom |
| 264 | 1 | |a Erlangen ; Nürnberg |c [2019] | |
| 300 | |a 77, VII Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2019 | ||
| 610 | 2 | 7 | |a Serotonin |0 (DE-588)10071510-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Parkinson-Krankheit |0 (DE-588)4044683-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Axon |0 (DE-588)4200576-0 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Axon |0 (DE-588)4200576-0 |D s |
| 689 | 0 | 2 | |a Serotonin |0 (DE-588)10071510-2 |D b |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-030936339 | |
Datensatz im Suchindex
| _version_ | 1819299218473353216 |
|---|---|
| adam_text | CONTENTS
LIST
OF
FIGURES
......................................................................................
I
LIST
OF
TABLES
.......................................................................................
II
1
DECLARATION
........................................................................................
III
2
SUMMARY/ZUSAMMENFASSUNG
.........................................................
1
2.1
SUMMARY
..........................................................................................................
1
2.2
ZUSAMMENFASSUNG
........................................................................................
3
3
INTRODUCTION
.......................................................................................
5
3.1
PARKINSON
*
S
DISEASE
.......................................................................................
5
3.1.1
CLINIC
.........................................................................................................
5
3.1.2
EPIDEMIOLOGY
............................................................................................
5
3.1.3
THERAPY
.....................................................................................................
6
3.1.4
NEUROPATHOLOGY
.........................................................................................
6
3.1.5
PATHOGENESIS
............................................................................................
9
3.2
HUMAN
STEM
CELLS
........................................................................................
12
3.2.1
ADULT
NEUROGENESIS
.................................................................................
12
3.2.2 INDUCED
PLURIPOTENT
STEM
CELLS
................................................................
14
3.2.3
IPSC
MODELS
OF
PD
................................................................................
14
3.2.4
STEM
CELL
THERAPY
IN
NEURODEGENERATIVE
DISEASES
.................................
15
3.3
HYPOTHESIS
OF
THE
THESIS
...............................................................................
16
4
MATERIAL
.............................................................................................
18
4.1
CHEMICALS
.....................................................................................................
16
4.2
KITS
AND
MASTER
MIXES
..................................................................................
18
4.3
PROTEIN
STANDARDS
.........................................................................................
18
4.4
BUFFERS
AND
SOLUTIONS
....................................................................
18
4.5
CELL
CULTURE
MEDIA
........................................................................................
20
4.6
REAGENTS
AND
DISPOSABLES
..........................................................................
22
4.7
PRIMARY
ANTIBODIES
.......................................................................................
23
4.8
SECONDARY
ANTIBODIES
.................................................................................
24
4.9
TECHNICAL
DEVICES
........................................................................................
25
4.10
SOFTWARE
...................................
.
.......
26
5
METHODS
.................................................................
27
5.1
ANIMAL
EXPERIMENTS
......................................................................................
27
5.1.1
TRANSGENIC
MICE
......................................................................................
27
5.1.2
HISTOLOGY
AND
LIGHT
MICROSCOPY
............................................................... 27
5.1.3
HIGH
PRESSURE
LIQUID
CHROMATOGRAPHY
....................................................
28
5.1.4
PROTEIN
EXTRACTION
AND
WESTERN
BLOT
.......................................................29
5.2
CELL
CULTURE
....................................................................................................
29
5.2.1
GENERATION
OF
INDUCED
PLURIPOTENT
STEM
CELLS
........................................
29
5.2.2
MIDBRAIN
DIFFERENTIATION
USING
SMALL
MOLECULES
......................................
30
5.2.3
IMMUNOCYTOCHEMISTRY
.............................................................................
31
5.2.4 FLOW
CYTOMETRY
........................................................................................
31
5.2.5
ANALYSIS
OF
NEUNTE
MORPHOLOGY
..............................................................
31
5.2.6
LIFE
CELL
IMAGING
(AXONAL
TRANSPORT,
CALCIUM
IMAGING)
...........................
32
5.2.7
QUANTIFICATION
OF
MITOCHONDRIA
................................................................
34
5.2.8
QUANTIFICATION
OF
SYNAPSES
.....................................................................
34
5.2.9
RNA
EXTRACTION
...........................................................
34
5.2.10
REVERSE
TRANSCRIPTION
AND
REAL
TIME-POLYMERASE
CHAIN
REACTION
..........
34
5.3
STATISTICS
.........................................................................................................
35
6
RESULTS
..............................................................................................
36
6.1
IMPAIRED
SEROTONERGIC
SYSTEM
IN
THE
A53T
ALPHA-SYNUCLEIN
MOUSE
MODEL
OF
PD
.....................................................................................................................
36
6.1.1
EXPRESSION
OF
ALPHA-SYNUCLEIN
IN
BRAINSTEM
SEROTONERGIC
NEURONS
.......
37
6.1.2
SEROTONERGIC
NEURONS
ARE
NOT
REDUCED
IN
A53T
ALPHA-SYNUCLEIN
MICE
37
6.1.3
REDUCED
LEVELS
OF
SEROTONIN
IN
THE
BRAINSTEM
OF
A53T
ALPHA-SYNUCLEIN
MICE
..................................................................................................................
38
6.1.4
CHARACTERIZATION
OF
THE
SEROTONERGIC
INNERVATION
INTO
THE
HIPPOCAMPAL
FORMATION
...........................................................................................................
38
6.1.5
ALPHA-SYNUCLEIN
IS
PRESENT
IN
HIPPOCAMPAL
SEROTONERGIC
FIBERS
.............40
6.1.6
REDUCED
SEROTONERGIC
INNERVATION
OF
THE
DORSAL
DG
.............................
40
6.1.7
SERT
EXPRESS
IO
N
IN
THE
BRAIN
STEM
OF
TRANSGENIC
MICE
........................
41
6.1.8
REDUCED
RESPONSE
TO
FLUOXETINE
TREATMENT
IN
TRANSGENIC
MICE
........... 42
6.2
STRUCTURAL
AND
FUNCTIONAL
ANALYSIS
OF
NEUNTES
IN
SPORADIC
PD
PATIENTS-
DERIVED
NEURONS
..........................
44
6.2.1
CHARACTERIZATION
OF
SPORADIC
PD
PATIENT-DERIVED
IPSC
......................... 44
6.2.2
GENERATION
AND
CHARACTERIZATION
OF
NEURAL
PROGENITOR
CELLS
..................
47
6.2.3
NEURONAL
DIFFERENTIATION
POTENTIAL
OF
CTRL
AND
PD-DERIVED
NPCS
.........
47
6.2.4
REDUCED
NEU
RITE
LENGTH
IN
PD
PATIENT-DERIVED
MIDBRAIN
NEURONS
.......
50
6.2.5
AXONAL
TRANSPORT
OF
MITOCHONDRIA
IN
CTRL-
AND
PD-DERIVED
LINES
..........
51
6.2.6
DISTRIBUTION
OF
MITOCHONDRIA
ALONG
AXONS
IN
CTRL-
AND
PD-DERIVED
LINES
52
6.2.7
CTRL-
AND
PD-DERIVED
NEURONS
FORM
SYNAPTIC
CONTACTS
.........................
53
6.2.8
SPONTANEOUS
ACTIVITY
AND
EXCITABILITY
OF
IPSC-DERIVED
NEURONS
...........
54
7
DISCUSSION
.......................................................................................
56
7.1
AXONAL
DEGENERATION
PRECEDES
LOSS
OF
SEROTONERGIC
RAPHE
NEURONS
IN
TRANSGENIC
MODELS
OF
PD
....................................................................................
56
7.2
A
POSSIBLE
LINK
BETWEEN
LOSS
OF
SEROTONERGIC
INNERVATION
INTO
THE
HIPPOCAMPAL
NEUROGENIC
NICHE
AND
NMS
IN
PD
............................................. 57
7.3
EARLY
AXONAL
DEGENERATION
-
THE
ROLE
OF
IMPAIRED
AXONAL
TRANSPORT
........
61
7.4
IPSC
MODELS
OF
PARKINSON
*
DISEASE
..........................................................
63
7.5
CHALLENGES
OF
MODELING
SPORADIC
PD
.........................................................
66
7.6
CONCLUSIONS
..................................................................................................
67
8
BIBLIOGRAPHY
....................................................................................
69
9
ABBREVIATIONS
....................................................................................
IV
10
ACKNOWLEDGEMENTS
.......................................................................
VI
|
| any_adam_object | 1 |
| author | Grosch, Janina |
| author_facet | Grosch, Janina |
| author_role | aut |
| author_sort | Grosch, Janina |
| author_variant | j g jg |
| building | Verbundindex |
| bvnumber | BV045552389 |
| ctrlnum | (OCoLC)1121487528 (DE-599)DNB1182063799 |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV045552389 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T18:30:09Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030936339 |
| oclc_num | 1121487528 |
| open_access_boolean | |
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| owner_facet | DE-29T DE-29 DE-355 DE-BY-UBR |
| physical | 77, VII Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spellingShingle | Grosch, Janina Axonal degeneration in Parkinson's disease = Axonale Degeneration beim Parkinson Syndrom Serotonin (DE-588)10071510-2 gnd Parkinson-Krankheit (DE-588)4044683-9 gnd Axon (DE-588)4200576-0 gnd |
| subject_GND | (DE-588)10071510-2 (DE-588)4044683-9 (DE-588)4200576-0 (DE-588)4113937-9 |
| title | Axonal degeneration in Parkinson's disease = Axonale Degeneration beim Parkinson Syndrom |
| title_alt | Axonale Degeneration beim Parkinson Syndrom |
| title_auth | Axonal degeneration in Parkinson's disease = Axonale Degeneration beim Parkinson Syndrom |
| title_exact_search | Axonal degeneration in Parkinson's disease = Axonale Degeneration beim Parkinson Syndrom |
| title_full | Axonal degeneration in Parkinson's disease = Axonale Degeneration beim Parkinson Syndrom vorgelegt von Janina Grosch (geb. Deußer) |
| title_fullStr | Axonal degeneration in Parkinson's disease = Axonale Degeneration beim Parkinson Syndrom vorgelegt von Janina Grosch (geb. Deußer) |
| title_full_unstemmed | Axonal degeneration in Parkinson's disease = Axonale Degeneration beim Parkinson Syndrom vorgelegt von Janina Grosch (geb. Deußer) |
| title_short | Axonal degeneration in Parkinson's disease |
| title_sort | axonal degeneration in parkinson s disease axonale degeneration beim parkinson syndrom |
| title_sub | = Axonale Degeneration beim Parkinson Syndrom |
| topic | Serotonin (DE-588)10071510-2 gnd Parkinson-Krankheit (DE-588)4044683-9 gnd Axon (DE-588)4200576-0 gnd |
| topic_facet | Serotonin Parkinson-Krankheit Axon Hochschulschrift |
| url | http://d-nb.info/1182063799/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030936339&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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