Influence of chromatin remodeler Ep400 on oligodendroglial development in mice: = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Erlangen ; Nürnberg
[2019]
|
| Schlagwörter: | |
| Links: | http://d-nb.info/1182469736/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030935156&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 128 Seiten Illustrationen, Diagramme 21 cm |
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| 100 | 1 | |a Elsesser, Olga |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Influence of chromatin remodeler Ep400 on oligodendroglial development in mice |b = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus |c vorgelegt von Olga Elsesser |
| 246 | 1 | 3 | |a Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus |
| 246 | 1 | 1 | |a Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus |
| 264 | 1 | |a Erlangen ; Nürnberg |c [2019] | |
| 300 | |a 128 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2019 | ||
| 650 | 0 | 7 | |a Chromatinremodellierung |0 (DE-588)1027359612 |2 gnd |9 rswk-swf |
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| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Oligodendrocyt |0 (DE-588)4309981-6 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Chromatinremodellierung |0 (DE-588)1027359612 |D s |
| 689 | 0 | 1 | |a Proteine |0 (DE-588)4076388-2 |D s |
| 689 | 0 | 2 | |a Zelldifferenzierung |0 (DE-588)4067536-1 |D s |
| 689 | 0 | 3 | |a Oligodendrocyt |0 (DE-588)4309981-6 |D s |
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| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030935156&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-030935156 | |
Datensatz im Suchindex
| _version_ | 1819270117933973504 |
|---|---|
| adam_text | TABLE
OF
CONTENT
TABLE
OF
CONTENT
........................................................................................................................
1
LIST
OF
FIGURES
............................................................................................................................
5
LIST
OF
TABLES
.............................................................................................................................
8
ZUSAMMENFASSUNG
.....................................................................................................................
9
SUMMARY
..................................................................................................................................
9
1
INTRODUCTION
..........................................................................................................
12
1.1
MYELINATION
IN
VERTEBRATES
....................................................................................
12
1.2
COMPOSITION
OF
A
MYELINATED
FIBER
........................................................................
12
1.3
GLIAL
CELLS
OF
THE
CNS
...........................................................................................
13
1.4
PRECURSOR
CELL
SPECIFICATION
IN
THE
NEURAL
TUBE
......................................................
14
1.5
THE TRANSCRIPTIONAL
PROGRAM
OF
OLIGODENDROGLIA
...................................................
16
1.5.1
TRANSCRIPTIONAL
REGULATION
OF
OLIGODENDROGLIAL
PROLIFERATION
...............................
16
1.5.2
TRANSCRIPTIONAL
REGULATION
OF
OLIGODENDROCYTE
DIFFERENTIATION
AND
MYELINATION
.
18
1.6
THE
CHROMATIN
STRUCTURE
AND
ITS
EPIGENETIC
MODULATION
.......................................
21
1.6.1
POST-TRANSLATIONAL
MODIFICATIONS
............................................................................
22
1.6.2
CHROMATIN
REMODELING
COMPLEXES
........................................................................
23
1.7
EPIGENETIC
MECHANISMS
DURING
OLIGODENDROGLIAL
DEVELOPMENT
...........................
25
1.7.1
EPIGENETIC
MECHANISMS
DURING
OLIGODENDROGLIAL
SPECIFICATION
AND
PROLIFERATION...
25
1.7.2
EPIGENETIC
MECHANISMS
DURING
OLIGODENDROGLIAL
DIFFERENTIATION
.........................
26
1.8
EP400
-
THE
CATALYTIC
SUBUNIT
OF
THE
TIP60/EP400
COMPLEX
.................................
27
2
AIM
OF
THE
CURRENT
STUDY
....................................................................................
30
3
RESULTS
...................................................................................................................
30
3.1
DELETION
OF
EP400
WITH
SOXLO::CRE
......................................................................
32
3.2
DELETION
OF
EP400
WITH
CNP1
::CRE
......................................................................
34
TABLE
OF
CONTENT
2
3.2.1
EFFICIENCY
OF
THE
CNP1
::CRE-MEDIATED
EP400
DELETION
IN
OLIGODENDROGLIAL
CELLS..
34
3.2.2
SURVIVAL
AND
ULTRASTRUCTURAL
MYELIN
ANALYSIS
OF
EP400AC
ANIMALS
35
3.2.3
POSTNATAL
EXPRESSION
PATTERN
OF
PAN-OLIGODENDROGLIAL
MARKER
PROTEINS
IN
THE
EP400AC
SPINAL
CORD
36
3.2.4
INVESTIGATIONS
ON
PROLIFERATION
AND
APOPTOSIS
IN
THE
EP400AC
SPINAL
CORD
38
3.2.5
DIFFERENTIATION
CAPACITY
OF
EP400-DEFICIENT
OLIGODENDROCYTES
40
3.2.6
SEVERELY
REDUCED
EXPRESSION
OF
MYELIN
GENES
IN
THE
EP400-DEFICIENT
SPINAL
CORD.
41
3.2.7
INCREASED
ASTROGLIOSIS
IN
THE
EP400-DEFICIENT
SPINAL
CORD
42
3.2.8
ANALYSIS
OF
THE
POTENTIAL
JOINED
INFLUENCE
OF
TWO
ATPASES
ON
OLIGODENDROGLIAL
DEVELOPMENT
43
3.2.9
ANALYSIS
CONCERNING
A
GENETIC
INTERACTION
BETWEEN
EP400
AND
SOXLO
45
3.2.10
EP400
AND
SOXLO
INTERACTION
ON
PROTEIN
LEVEL
47
3.2.11
CO-PRESENCE
OF
EP400
AND
SOXLO
ON
THE
MYRF
GENE
50
3.2.12
FUNCTIONAL
RELEVANCE
OF
THE
EP400-SOXL0
INTERACTION
AT
THE
ECR9
OF
MYRF
53
3.2.13
CHANGES
IN
THE
TRANSCRIPTOME
OF
OLIGODENDROGLIA
IN
THE
ABSENCE
OF
EP400
54
3.2.14
INFLUENCE
OF
EP400
DELETION
IN
THE
FOREBRAIN
57
3.3
EP400
IS
NOT
IMPORTANT
FOR
MYELIN
MAINTENANCE
58
4
DISCUSSION
61
4.1
EP400
AND
THE
MYELINATION
PROCESS
IN
OLIGODENDROCYTES
62
4.2
OLIGODENDROCYTE
DIFFERENTIATION
DEPENDS
ON
EP400
64
4.3
EP400
AND
ITS
CONTRIBUTION
TO
OLIGODENDROGLIAL
GENE
ACTIVATION
66
4.3.1
EP400
AND
ITS
INVOLVEMENT
IN
MYRF
ACTIVATION
67
4.4
THE
PRO-SURVIVAL
ROLE
OF
EP400
IN
OLIGODENDROCYTES
.......................
68
4.4.1
ACCUMULATION
OF
DSBS
UPON
EP400
DELETION
69
4.4.2
INCREASED
OLIGODENDROGLIAL
CELL
DEATH
DUE
TO
EP400
LOSS
72
4.5
HETEROGENEITY
IN
OLIGODENDROGLIA
72
TABLE
OF
CONTENT
3
4.6
CHROMATIN
REMODELING
DURING
OLIGODENDROCYTE
DIFFERENTIATION
............................
73
4.6.1
REDUNDANCY
BETWEEN
CHROMATIN
REMODELING
COMPLEXES
.....................................
73
4.6.2
THE
GENERAL
IMPORTANCE
OF
CHROMATIN
REMODELING
FOR
MYELINATING
GLIA
CELLS
....
75
5
MATERIAL
&
METHODS
..............................................................................................
76
5.1
MATERIAL
..................................................................................................................
76
5.1.1
MOUSE
LINES
............................................................................................................
76
5.1.2
REAGENTS
AND
CHEMICALS
........................................................................................
76
5.1.3
OLIGONUCLEOTIDES
...................................................................................................
82
5.1.4
PROBES
FOR
IN
SITU
HYBRIDIZATION
.............................................................................
83
5.1.5
ANTIBODIES
..............................................................................................................
83
5.1.6
PLASMIDS
.................................................................................................................
84
5.2
METHODS
..................................................................................................................
85
5.2.1
STANDARD
METHODS
..................................................................................................
85
5.2.2
ANIMAL
HUSBANDRY
.................................................................................................
85
5.2.3
ISOLATION
OF
GENOMIC
DNA
FOR
GENOTYPING
..........................................................
85
5.2.4
GENOTYPING
.............................................................................................................
86
5.2.5
TISSUE
PREPARATION
.................................................................................................
88
5.2.6
PREPARATION
OF
FROZEN
SECTIONS
...............................................................................
88
5.2.7
IMMUNOHISTOCHEMICAL
STAINING
OF
CRYOSECTIONS
...................................................
88
5.2.8
IN
SITU
HYBRIDIZATION
..............................................................................................
91
5.2.9
ELECTRON
MICROSCOPIC
ANALYSES
..............................................................................
92
5.2.10 CELL
CULTURE
............................................................................................................
92
5.2.11
TRANSFECTION
OF
HEK293
CELLS
FOR
WHOLE
CELL
EXTRACT
..........................................
94
5.2.12
TRANSFECTION
OF
33B
CELLS
FOR
LUCIFERASE
ASSAY
.....................................................
94
5.2.13
LUCIFERASE
ASSAY
....................................................................................................
94
5.2.14
CO-IMMUNOPRECIPITATION
(CO-DP)
..........................................................................
95
5.2.15
GST-PULLDOWN
.......................................................................................................
95
5.2.16
SODIUM
DODECYL
SULFATE
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
(SDS-PAGE)
......
95
TABLE
OF
CONTENT
4
5.2.17
WESTERN
BLOT
...........................................................................................................
96
5.2.18
FACS
......................................................................................................................
96
5.2.19
RNA
ISOLATION
........................................................................................................
97
5.2.20
RNA
SEQUENCING
....................................................................................................
97
5.2.21
CHROMATIN
IMMUNOPRECIPITATION
............................................................................
97
5.2.22
QPCR
......................................................................................................................
98
5.2.23
EDITING
AND
STATISTICAL
ANALYSIS
...............................................................................
99
ABBREVIATIONS
.........................................................................................................................
100
BIBLIOGRAPHY
...........................................................................................................................
105
DANKSAGUNG
............................................................................................................................
128
|
| any_adam_object | 1 |
| author | Elsesser, Olga |
| author_facet | Elsesser, Olga |
| author_role | aut |
| author_sort | Elsesser, Olga |
| author_variant | o e oe |
| building | Verbundindex |
| bvnumber | BV045551181 |
| ctrlnum | (OCoLC)1121486010 (DE-599)DNB1182469736 |
| discipline | Biologie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV045551181 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T18:30:07Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030935156 |
| oclc_num | 1121486010 |
| open_access_boolean | |
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| owner_facet | DE-29T DE-29 DE-355 DE-BY-UBR |
| physical | 128 Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2019 |
| publishDateSearch | 2019 |
| publishDateSort | 2019 |
| record_format | marc |
| spellingShingle | Elsesser, Olga Influence of chromatin remodeler Ep400 on oligodendroglial development in mice = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus Chromatinremodellierung (DE-588)1027359612 gnd Zelldifferenzierung (DE-588)4067536-1 gnd Proteine (DE-588)4076388-2 gnd Oligodendrocyt (DE-588)4309981-6 gnd |
| subject_GND | (DE-588)1027359612 (DE-588)4067536-1 (DE-588)4076388-2 (DE-588)4309981-6 (DE-588)4113937-9 |
| title | Influence of chromatin remodeler Ep400 on oligodendroglial development in mice = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus |
| title_alt | Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus |
| title_auth | Influence of chromatin remodeler Ep400 on oligodendroglial development in mice = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus |
| title_exact_search | Influence of chromatin remodeler Ep400 on oligodendroglial development in mice = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus |
| title_full | Influence of chromatin remodeler Ep400 on oligodendroglial development in mice = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus vorgelegt von Olga Elsesser |
| title_fullStr | Influence of chromatin remodeler Ep400 on oligodendroglial development in mice = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus vorgelegt von Olga Elsesser |
| title_full_unstemmed | Influence of chromatin remodeler Ep400 on oligodendroglial development in mice = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus vorgelegt von Olga Elsesser |
| title_short | Influence of chromatin remodeler Ep400 on oligodendroglial development in mice |
| title_sort | influence of chromatin remodeler ep400 on oligodendroglial development in mice der einfluss des chromatin remodellierenden ep400 auf die entwicklung der oligodendrozyten in der maus |
| title_sub | = Der Einfluss des Chromatin-remodellierenden Ep400 auf die Entwicklung der Oligodendrozyten in der Maus |
| topic | Chromatinremodellierung (DE-588)1027359612 gnd Zelldifferenzierung (DE-588)4067536-1 gnd Proteine (DE-588)4076388-2 gnd Oligodendrocyt (DE-588)4309981-6 gnd |
| topic_facet | Chromatinremodellierung Zelldifferenzierung Proteine Oligodendrocyt Hochschulschrift |
| url | http://d-nb.info/1182469736/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030935156&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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