PCR Protocols: Current Methods and Applications
PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a w...
Gespeichert in:
| Weitere beteiligte Personen: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | Englisch |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1993
|
| Schriftenreihe: | Methods in Molecular Biology
15 |
| Schlagwörter: | |
| Links: | https://doi.org/10.1385/0896032442 https://doi.org/10.1385/0896032442 |
| Zusammenfassung: | PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR. |
| Umfang: | 1 Online-Ressource (XIV, 392 p) |
| ISBN: | 9781592595020 |
| DOI: | 10.1385/0896032442 |
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| 520 | |a PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR. | ||
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Datensatz im Suchindex
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| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Medizin |
| doi_str_mv | 10.1385/0896032442 |
| format | Electronic eBook |
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| id | DE-604.BV044951328 |
| illustrated | Not Illustrated |
| indexdate | 2024-12-20T18:15:05Z |
| institution | BVB |
| isbn | 9781592595020 |
| language | English |
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| physical | 1 Online-Ressource (XIV, 392 p) |
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| publishDate | 1993 |
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| publisher | Humana Press |
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| series2 | Methods in Molecular Biology |
| spelling | PCR Protocols Current Methods and Applications edited by Bruce A. White Totowa, NJ Humana Press 1993 1 Online-Ressource (XIV, 392 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 15 PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR. Life Sciences Cell Biology Life sciences Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 White, Bruce A. edt Erscheint auch als Druck-Ausgabe 9780896032446 https://doi.org/10.1385/0896032442 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | PCR Protocols Current Methods and Applications Life Sciences Cell Biology Life sciences Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4256726-9 |
| title | PCR Protocols Current Methods and Applications |
| title_auth | PCR Protocols Current Methods and Applications |
| title_exact_search | PCR Protocols Current Methods and Applications |
| title_full | PCR Protocols Current Methods and Applications edited by Bruce A. White |
| title_fullStr | PCR Protocols Current Methods and Applications edited by Bruce A. White |
| title_full_unstemmed | PCR Protocols Current Methods and Applications edited by Bruce A. White |
| title_short | PCR Protocols |
| title_sort | pcr protocols current methods and applications |
| title_sub | Current Methods and Applications |
| topic | Life Sciences Cell Biology Life sciences Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Polymerase-Kettenreaktion |
| url | https://doi.org/10.1385/0896032442 |
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