Multi-parameter protein analysis with an electro-switchable biosurface:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Garching
Verein zur Förderung des Walter Schottky Instituts der Technischen Universität München e.V.
2015
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Selected topics of semiconductor physics and technology
Vol. 194 |
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028732351&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | viii, 106 Seiten Diagramme |
| ISBN: | 9783941650947 |
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| 245 | 1 | 0 | |a Multi-parameter protein analysis with an electro-switchable biosurface |c Andreas Langer |
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| 264 | 1 | |a Garching |b Verein zur Förderung des Walter Schottky Instituts der Technischen Universität München e.V. |c 2015 | |
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Datensatz im Suchindex
| DE-BY-TUM_call_number | 0001 DM 33770 |
|---|---|
| DE-BY-TUM_katkey | 2165993 |
| DE-BY-TUM_location | Mag |
| DE-BY-TUM_media_number | TEMP3248084 |
| _version_ | 1821933939386744832 |
| adam_text | CONTENTS
ABSTRACT.
ZUSAMMENFASSUNG.......................................................................................................................................
I*I
PUBLICATIONS....................................................................................................................................................
*
CONTENTS.......................................................................................................................................................
VII
1
INTRODUCTION...............................................................................................................................................1
2 PROTEIN ANALYSIS BY T*ME-RESOLVED MEASUREMENTS
..........................................................................
3
2.1
INTRODUCTION............................................................................................................................
3
2.2 TIME-RESOLVED DNA
SWITCHING...............................................................................................
4
2.3 PROTEIN DETECTION AND PROTEIN BINDING
KINETICS....................................................................6
2.4
CONCLUSION...............................................................................................................................
8
5 MOLECULAR DYNAMICS OF DNA-PROTEIN COMPLEXES ON ELECTRIFIED
SURFACES................................. 9
3.1
INTRODUCTION............................................................................................................................
9
3.2 DNA-THE MOLECULE OF
*SE...................................................................................................
10
3.3 PROTEINS - THE WORKHORSES OF
LIFE........................................................................................
12
3.4 FREE ENERGY OF A DNA CYLINDER TETHERED TO A CHARGED
SURFACE...........................................12
3.5 DNA ORIENTATION IN
EQUILIBRIUM.............................................................................................
14
3.6 MOLECULAR DYNAMICS OF DNA IN AN ALTERNATING ELECTRIC
FIELD................................................17
3.7 DYNAMICS OF A DNA-PROTEIN *LOLLIPOP* COMPLEX
..................................................................
21
3.8 COMPARISON TO EXPERIMENTS WITH ELECTRICALLY SWITCHABLE DNA
..........................................
23
3.9
CONCLUSION.............................................................................................................................
28
4 PROTEIN SIZE AND CONFORMATION
ANALYSIS..........................................................................................29
4.1
INTRODUCTION..........................................................................................................................
29
4.2 STEADY-STATE AND DYNAMICS: THEORY VS.
EXPERIMENT...........................................................30
4.3 DETERMINATION OF HYDRODYNAMIC PROTEIN
DIAMETERS...........................................................32
4.4 CONFORMATION
ANALYSIS.........................................................................................................
35
4.5 DIRECT DETECTION OF SMALL
MOLECULES...................................................................................
36
4.6
CONCLUSION.............................................................................................................................
36
5 PROTEIN-SMALL MOLECULE
INTERACTIONS..................................................................................................4*
5.1
INTRODUCTION...........................................................................................................................
41
5.2 SAMPLE
PREPARATION...............................................................................................................
42
5.3 DIRECT INTERACTION
ANALYSIS....................................................................................................44
5.4 INDIRECT INTERACTION
ANALYSIS.................................................................................................46
5.5 CONFORMATION
ANALYSIS..........................................................................................................
52
5.6
CONCLUSION..............................................................................................................................55
6 POLYME*ASE-DNA INTERACTIONS AND MEASUREMENT OF ENZYMATIC
ACTIVITY***********************57
6.1
INTRODUCTION...........................................................................................................................
57
6.2 MEASUREMENT
PRINCIPLES........................................................................................................60
6.3 KINETICS OF THE TAQ-DNA
INTERACTION....................................................................................62
6.4 THERMODYNAMICS OF
TAQ........................................................................................................64
6.5 ENZYMATIC ACTIVITY OF
TAQ......................................................................................................66
6.6 KINETICS AND AFFINITIES OF POL
L(KF)........................................................................................68
6.7 ENZYMATIC ACTIVITY OF POL
L(KF)..............................................................................................69
6.8 DETECTION OF THE FINGERS-CLOSING CONFORMATIONAL TRANSITION
.............................................
72
6.9
CONDUSION..............................................................................................................................74
7 CONCLUSION AND
OUTLOOK........................................................................................................................
77
A
BIOMOLECULES............................................................................................................................................81
*.1 DNA
SEQUENCES......................................................................................................................
81
*.2
PROTEINS..................................................................................................................................82
B
METHODS....................................................................................................................................................83
*.1 CHIP AND DNA LAYER
PREPARATION..........................................................................................83
*.2 MODIFICATION OF DNA WITH CAPTURE MOLECULES AND
PROTEINS...............................................84
*.3 TCPSC SETUP AND DNA SWITCHING
POTENTIALS........................................................................
85
*.4 DETERMINATION OF PROTEIN DIAMETERS BY DLS AND
XRD........................................................86
*.5 DHFR
MEASUREMENTS.............................................................................................................86
*.6 POLYMERASE
MEASUREMENTS...................................................................................................87
C ADDITIONAL MEASUREMENT
DATA.............................................................................................................89
C.1 DNA SWITCHING IN UREA AND
GLYCEROL.....................................................................................89
C.2 DHFR TITRATION
MEASUREMENTS.............................................................................................90
C
*
3 POLYMERASE BINDING TO DSDNA WITH AN INTERNAI
LABEL.........................................................94
BIBLIOGRAPHY.................................................................................................................................................
95
ACKNOWLEDGEMENTS...................................................................................................................................
105
|
| any_adam_object | 1 |
| author | Langer, Andreas |
| author_facet | Langer, Andreas |
| author_role | aut |
| author_sort | Langer, Andreas |
| author_variant | a l al |
| building | Verbundindex |
| bvnumber | BV043311718 |
| classification_tum | ELT 300d PHY 685d TEC 030d |
| ctrlnum | (OCoLC)945172117 (DE-599)BVBBV043311718 |
| discipline | Physik Technik Elektrotechnik / Elektronik / Nachrichtentechnik |
| edition | 1. Auflage |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV043311718 |
| illustrated | Not Illustrated |
| indexdate | 2024-12-20T17:33:26Z |
| institution | BVB |
| isbn | 9783941650947 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028732351 |
| oclc_num | 945172117 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-12 DE-83 |
| owner_facet | DE-91 DE-BY-TUM DE-12 DE-83 |
| physical | viii, 106 Seiten Diagramme |
| publishDate | 2015 |
| publishDateSearch | 2015 |
| publishDateSort | 2015 |
| publisher | Verein zur Förderung des Walter Schottky Instituts der Technischen Universität München e.V. |
| record_format | marc |
| series | Selected topics of semiconductor physics and technology |
| series2 | Selected topics of semiconductor physics and technology |
| spellingShingle | Langer, Andreas Multi-parameter protein analysis with an electro-switchable biosurface Selected topics of semiconductor physics and technology |
| subject_GND | (DE-588)4113937-9 |
| title | Multi-parameter protein analysis with an electro-switchable biosurface |
| title_auth | Multi-parameter protein analysis with an electro-switchable biosurface |
| title_exact_search | Multi-parameter protein analysis with an electro-switchable biosurface |
| title_full | Multi-parameter protein analysis with an electro-switchable biosurface Andreas Langer |
| title_fullStr | Multi-parameter protein analysis with an electro-switchable biosurface Andreas Langer |
| title_full_unstemmed | Multi-parameter protein analysis with an electro-switchable biosurface Andreas Langer |
| title_short | Multi-parameter protein analysis with an electro-switchable biosurface |
| title_sort | multi parameter protein analysis with an electro switchable biosurface |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028732351&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV011499438 |
| work_keys_str_mv | AT langerandreas multiparameterproteinanalysiswithanelectroswitchablebiosurface |
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