Molecular structure-processing relationships of proteins in solution and interfaces:
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| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2014
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| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028061174&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 264 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Molecular structure-processing relationships of proteins in solution and interfaces |c vorgelegt von Ronald Gebhardt |
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Datensatz im Suchindex
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| adam_text | MOLECULAR STRUCTURE-PROCESSING RELATIONSHIPS OF PROTEINS IN
SOLUTION AND AT INTERFACES
LEHRSTUHL FUR LEBENSMITTELVERFAHRENSTECHNIK UND MOLKEREITECHNOLOGIE
DEPARTMENT FUR INGENIEURWISSENSCHAFTEN FUR LEBENSMITTEL UND
BIOGENE ROHSTOFFE
FAKULTAT WISSENSCHAFTSZENTRUM WEIHENSTEPHAN FTIR
ERNAHRUNG, LANDNUTZUNG UND UMWELT
FREISING-WEIHENSTEPHAN
HABILITATIONSSCHRIFT
VORGELEGT VON
DR. RER. NAT. RONALD GEBHARDT
TECHNISCHE UNIVERSITAT MIINCHEN
2014
HTTP://D-NB.INFO/1073539652
2
CONTENTS
TABLE OF CONTENTS
INTRODUCTION 1 11
1.1. STRUCTURE OF P-LACTOGLOBULIN 12
1.2. STRUCTURE OF A SINGLE CASEIN MICELLE 13
1.3. STRUCTURE OF CASEIN MICELLE ENSEMBLES IN FILMS AND DEPOSITS 15
1.4. CURRENT KNOWLEDGE ABOUT THE STRUCTURE AND INTERACTIONS OF CM AND
P-LACTOGLOBULIN IN SOLUTION AND DEPOSITS 16
1.5. HIGH PRESSURE TREATMENT 17
1.6. PROTEIN FILMS 18
1.7. MEMBRANE FILTRATION 19
1.8. GRAZING INCIDENCE SMALL ANGLE X-RAY SCATTERING (GISAXS) 20
RESULTS AND CONTRIBUTIONS 2
22
2.1. HIGH PRESSURE STABILITY OF PROTEIN COMPLEXES STUDIED BY STATIC AND
DYNAMIC
LIGHT SCATTERING 22
2.1.1. INTRODUCTION 22
2.1.2. MATERIALS AND METHODS 24
2.1.2.1. SAMPLE PREPARATION 24
2.1.2.2. HIGH PRESSURE LIGHT SCATTERING SET-UP 24
2.1.2.3. STATIC AND DYNAMIC LIGHT SCATTERING: 25
2.1.3. RESULTS 26
2.1.3.1. 12-MER OF HOMARUS AMERICANUS 26
2.1.3.2. EFFECT OF TEMPERATURE AND CALCIUM ON THE STABILITY OF CASEIN
MICELLES 28
2.1.4. DISCUSSION 30
2.1.5. CONCLUSION 31
2.2. STRUCTURE AND STABILIZING INTERACTIONS OF CASEIN MICELLES PROBED BY
HIGH PRESSURE
DISSOCIATION 33
2.2.1. INTRODUCTION 33
2.2.2. MATERIALS AND METHODS 35
2.2.2.1. SAMPLE PREPARATION 35
2.2.2.2. LIGHT SCATTERING EXPERIMENTS 35
2.2.2.3. FTIR SPECTROSCOPY WITH A DIAMOND ANVIL PRESSURE CELL 37
3
CONTENTS
2.2.3. RESULTS 38
2.2.3.1. LIGHT SCATTERING 38
2.2.3.2. PRESSURE DISSOCIATION AS A PROBE OF THE TEMPERATURE-DEPENDENT
STABILITY OF
CASEIN MICELLES 41
2.2.3.3. PRESSURE DISSOCIATION AS A PROBE OF CA-DEPENDENT STABILITY OF
CASEIN MICELLES 43
2.2.3.4. SYNERGISTIC EFFECTS OF TEMPERATURE AND CALCIUM CONCENTRATION ON
THE STABILITY OF
CASEIN MICELLES 44
2.2.3.5. EFFECT OF PH ON PRESSURE STABILITY OF CASEIN MICELLES 45
2.2.3.6. DATA ANALYSIS; THE IRREVERSIBLE DISSOCIATION MODEL 46
2.2.3.7. HIGH PRESSURE FTIR SPECTROSCOPY OF CASEIN MICELLES 51
2.2.4. DISCUSSION AND CONCLUSION 53
2.3. PRESSURE DISSOCIATION OF P-LACTOGLOBULIN OLIGOMERS NEAR THEIR
ISOELECTRIC POINT 56
2.3.1. INTRODUCTION 56
2.3.2. MATERIALS AND METHODS 58
2.3.2.1. PREPARATION OF |I-LG 58
2.3.2.2. HP-DLS/SLS AND RP-HPLC 58
2.3.2.3. EXPERIMENTAL SETUP 58
2.3.2.4. THEORETICAL BASIS 59
2.3.3. RESULTS 60
2.3.4. DISCUSSION 66
2.3.5. CONCLUSIONS 68
2.4. EFFECT OF HYDROSTATIC PRESSURE TREATMENT ON THE STRUCTURE-FOAMING
RELATIONSHIPS OF P-
LACTOGLOBULIN 69
2.4.1. INTRODUCTION 69
2.4.2. MATERIAL AND METHODS 70
2.4.2.1. SAMPLE PREPARATION AND CHARACTERIZATION 70
2.4.2.2. DYNAMIC LIGHT SCATTERING 70
2.4.2.3. HIGH PRESSURE TREATMENT 72
2.4.2.4. CIRCULAR DICHROISM 72
2.4.2.5. FOAMING EXPERIMENTS 72
2.4.3. RESULTS AND DISCUSSION 73
2.4.4. CONCLUSION 78
4
CONTENTS
2.5. STRUCTURAL CHANGES OF CASEIN MICELLES IN A CALCIUM GRADIENT FILM 80
2.5.1. INTRODUCTION 80
2.5.2. EXPERIMENTAL PART 81
2.5.2.1. SAMPLE PREPARATION 81
2.5.2.2. MICRO BEAM GRAZING INCIDENCE SMALL ANGLE X-RAY SCATTERING 82
2.5.2.3. SYNCHROTRON RADIATION EXPERIMENTS 83
2.5.2.4. DATA ANALYSIS 83
2.5.3. RESULTS AND DISCUSSION 84
2.5.3.1.
JI
GISAXS OF CASEIN FILMS PREPARED BY SOLUTION CASTING 84
2.5.3.2. LINE CUTS THROUGH THE
JI
GISAXS INTENSITY DISTRIBUTION 86
2.5.3.3. PARTICLE SIZES 87
2.5.3.4. SIZE DISTRIBUTIONS 88
2.5.3.5. SOLUTION CASTING 91
2.5.4. CONCLUSIONS 92
2.6. INVESTIGATION OF SURFACE MODIFICATION OF CASEIN FILMS BY RENNIN
ENZYME ACTION USING MICRO-
BEAM GRAZING INCIDENCE SMALL ANGLE X-RAY SCATTERING 93
2.6.1. INTRODUCTION : 93
2.6.2. MATERIAL AND METHODS 94
2.6.2.1. SAMPLE PREPARATION 94
2.6.2.2. MICROBEAM GRAZING INCIDENCE SMALL ANGLE X-RAY SCATTERING 95
2.6.2.3. THEORETICAL BACKGROUND 96
2.6.3. RESULTS AND DISCUSSION 97
2.6.4. SUMMARY AND CONCLUSIONS 104
2.7. STRUCTURAL CHANGES OF CASEIN MICELLES IN A RENNIN GRADIENT FILM
WITH SIMULTANEOUS
CONSIDERATION OF THE FILM MORPHOLOGY 106
2.7.1. INTRODUCTION 106
2.7.2. EXPERIMENTAL SECTION 108
2.7.2.1. SAMPLE PREPARATION 108
2.7.2.2. MICRO-BEAM GRAZING INCIDENCE SMALL ANGLE X-RAY SCATTERING 109
2.7.2.3. DATA ANALYSIS 111
2.7.3. RESULTS AND DISCUSSION 112
2.7.3.1. RENNIN CONCENTRATION GRADIENT 112
2.7.3.2. PARTICLE SIZES AND SIZE DISTRIBUTIONS 113
2.7.3.3. CASEIN FILM SURFACE MORPHOLOGY AND ITS MICROSTRUCTURE 120
5
CONTENTS
2.7.4. CONCLUSIONS 122
2.8. STRUCTURAL CHARACTERIZATION OF CASEIN MICELLES: SHAPE CHANGES
DURING FILM FORMATION 124
2.8.1. INTRODUCTION 124
2.8.2. EXPERIMENTAL DETAILS 125
2.8.2.1. SAMPLE PREPARATION 125
2.8.2.2. DYNAMIC LIGHT SCATTERING (DLS) EXPERIMENTS 125
2.8.2.3. GRAZING INCIDENCE SMALL ANGLE X-RAY SCATTERING (GISAXS) 126
2.8.2.4. GISAXS DATA ANALYSIS 126
2.8.2.5. ATOMIC FORCE MICROSCOPY (AFM) 127
2.8.3. RESULTS 127
2.8.3.1. OPTICAL MICROSCOPY 127
2.8.3.2. AFM 127
2.8.3.3. GISAXS AND DLS 129
2.8.4. DISCUSSION 132
2.8.5. CONCLUSION 134
2.9. SIMULATION OF THE SHAPE AND SIZE OF CASEIN MICELLES IN A FILM STATE
135
2.9.1. INTRODUCTION 135
2.9.2. EXPERIMENTAL 136
2.9.2.1. THEORETICAL BACKGROUND 136
2.9.2.2. SIMULATION 137
2.9.2.3. SAMPLE PREPARATION 138
2.9.2.4. SURFACE-SENSITIVE EXPERIMENTS ON CASEIN FILMS 138
2.9.3. RESULTS AND DISCUSSION 139
2.9.4. CONCLUSIONS 145
2.10. P-LACTOGLOBULIN FILMS WITH SPIRAL CRACKS 146
2.10.1. INTRODUCTION 146
2.10.2. MATERIAL AND METHODS 148
2.10.2.1. WHEY PROTEINS 148
2.10.2.2. FILM FORMATION AND CHARACTERIZATION 148
2.10.3. RESULTS 150
2.10.3.1. SPIRAL CRACKS - INFLUENCE OF THE KIND OF WHEY PROTEIN, FOLDING
STATE AND UNDERLYING
SUBSTRATE 150
2.10.3.2. INFLUENCE OF THE PROTEIN CONCENTRATION 151
2.10.3.3. INFLUENCE OF THE PLASTICIZERS GLYCEROL AND SORBITOL 153
6
CONTENTS
2.10.4. DISCUSSION 155
/
2.10.5. CONCLUSIONS 160
2.11. STRUCTURAL ORDERING OF CASEIN MICELLES ON SILICON NITRIDE
MICRO-SIEVES
DURING FILTRATION 161
2.11.1. INTRODUCTION 161
2.11.2. MATERIALS AND METHODS 163
2.11.2.1. SAMPLE PREPARATION 163
2.11.2.2. LIGHT SCATTERING EXPERIMENTS 163
2.11.2.3. DEAD-END MICROFILTRATION 166
2.11.2.4. ATOMIC FORCE MICROSCOPY 166
2.11.3. RESULTS 166
2.11.4. DISCUSSION 171
2.11.5. CONCLUSION 173
2.12. STRUCTURAL CHANGES OF DEPOSITED CASEIN MICELLES INDUCED BY
MEMBRANE FILTRATION 174
2.12.1. INTRODUCTION 174
2.12.2. EXPERIMENTAL 176
2.12.2.1. MATERIALS . . 176
2.12.2.2. MICROFILTRATION CELL 176
2.12.2.3. GRAZING INCIDENCE SMALL ANGLE-X-RAY SCATTERING - GISAXS 176
2.12.2.4. DATA ANALYSIS 177
2.12.2.5. ATOMIC FORCE MICROSCOPY - AFM 178
2.12.3. RESULTS 178
2.12.3.1. GISAXS PATTERN OF CASEIN DEPOSITS AND MICRO-SIEVES 178
2.12.3.2. GISAXS SCAN ACROSS CASEIN DROPLET PROFILES 179
2.12.3.3. SIMULATION OF THE GISAXS PATTERN 182
2.12.3.4. AFM-MEASUREMENTS 185
2.12.4. DISCUSSION 186
2.12.5. CONCLUSIONS 188
2.13. EFFECT OF FILTRATION FORCES ON THE STRUCTURE OF CASEIN MICELLES
189
2.13.1. INTRODUCTION 189
2.13.2. MATERIAL AND METHODS 191
2.13.2.1. SAMPLE PREPARATION 191
2.13.2.2. FRONTAL FILTRATION 191
2.13.2.3. X-RAY-EXPERIMENTS 192
7
CONTENTS
2.13.2.4. FITTING MODEL 193
2.13.3. RESULTS AND DISCUSSION 194
2.13.3.1. POROUS VERSUS NON-POROUS AREAS 194
2.13.3.2. INFLUENCE OF THE TRANS-MEMBRANE PRESSURE 195
2.13.3.3. DEPOSITS OF CM IN A SINGLE PORE 197
2.13.4. CONCLUSIONS 198
2.14. STRUCTURE OF MILK PROTEIN DEPOSITS FORMED BY CASEIN MICELLES AND
P-LACTOGLOBULIN DURING
FRONTAL MICROFILTRATION 200
2.14.1. INTRODUCTION 200
2.14.2. MATERIAL AND METHODS 202
2.14.2.1. FILTRATION CELLS 202
2.14.2.2. PREPARATION OF FILTRATION FLUIDS 203
2.14.2.3. GISAXS EXPERIMENTS 203
2.14.2.4. DATA TREATMENT AND SIMULATIONS 203
2.14.3. RESULTS AND DISCUSSION 204
2.14.3.1. FRONTAL MICROFILTRATION EXPERIMENTS 204
2.14.3.2. FILM STRUCTURES ON NON-POROUS SUBSTRATES 205
2.14.3.3. DEPOSITS STRUCTURES ON MICRO-SIEVES FORMED BY FRONTAL
MICROFILTRATION 208
2.14.4. CONCLUSIONS 211
2.15. X-RAY SCATTERING ON CASEIN MICELLES AND HIGH DENSITY LIPOPROTEIN
(HDL) GRANULES DURING
PROCESSING OF FOODS 212
2.15.1. INTRODUCTION 212
2.15.2. EXPERIMENTAL 213
2.15.2.1. EGG YOLK GRANULES 213
2.15.2.2. CASEIN MICELLES (CM) 213
2.15.2.3. DIFFRACTION EXPERIMENTS 213
2.15.3. RESULTS 214
2.15.3.1. INTERNAL STRUCTURE OF CM IN MEMBRANE DEPOSITS 214
2.15.3.2. PHOSPHOLIPIDS IN THE HDL-GRANULES 215
-2.15.3.3. CONCLUSION 217
2.16. STRUCTURAL STUDY ON HEN-EGG YOLK HIGH DENSITY LIPOPROTEIN (HDL)
GRANULES 218
2.16.1. INTRODUCTION 218
2.16.2. MATERIAL AND METHODS 220
2.16.2.1. EGG YOLK PREPARATION 220
8
CONTENTS
2.16.2.2. SEDIMENTATION ANALYSIS 220
2.16.2.3. DENSITY MEASUREMENTS 221
2.16.2.4. ATOMIC FORCE MICROSCOPY
1
221
2.16.2.5. X-RAY SCATTERING EXPERIMENTS 222
2.16.2.6. DATA ANALYSIS 222
2.16.3. RESULTS AND DISCUSSION 222
2.16.4. CONCLUSION 229
SUMMARY & CONCLUSION 3
230
APPENDIX 235
REFERENCES 239
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| spellingShingle | Gebhardt, Ronald Molecular structure-processing relationships of proteins in solution and interfaces |
| subject_GND | (DE-588)4113937-9 |
| title | Molecular structure-processing relationships of proteins in solution and interfaces |
| title_auth | Molecular structure-processing relationships of proteins in solution and interfaces |
| title_exact_search | Molecular structure-processing relationships of proteins in solution and interfaces |
| title_full | Molecular structure-processing relationships of proteins in solution and interfaces vorgelegt von Ronald Gebhardt |
| title_fullStr | Molecular structure-processing relationships of proteins in solution and interfaces vorgelegt von Ronald Gebhardt |
| title_full_unstemmed | Molecular structure-processing relationships of proteins in solution and interfaces vorgelegt von Ronald Gebhardt |
| title_short | Molecular structure-processing relationships of proteins in solution and interfaces |
| title_sort | molecular structure processing relationships of proteins in solution and interfaces |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028061174&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT gebhardtronald molecularstructureprocessingrelationshipsofproteinsinsolutionandinterfaces |
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