Nanoliter-droplet thermophoresis for biomedical applications:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2014
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| Schlagwörter: | |
| Links: | http://d-nb.info/1056876654/34 https://nbn-resolving.org/urn:nbn:de:bvb:19-173039 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027526646&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 47, [57] S. Ill., graph. Darst. |
| Format: | Langzeitarchivierung gewährleistet, LZA |
Internformat
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Datensatz im Suchindex
| DE-BY-TUM_katkey | 2028894 |
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| _version_ | 1821935264520470529 |
| adam_text | NANOLITER-DROPLET THERMOPHORESIS
FOR BIOMEDICAL APPLICATIONS
ZUR ERLANGUNG DES GRADES
DOKTOR DER NATURWISSENSCHAFTEN (DR. RER. NAT.)
DER FAKULTAT FUR PHYSIK
DER LUDWIG-MAXIMILIANS-UNIVERSITAT MIINCHEN
VORGELEGT VON
SUSANNE ANJA IRENE SEIDEL
AUS SELB
DISSERTATION
MIINCHEN, JUNI 2014
HTTP://D-NB.INFO/1058456768
TABLE OF CONTENTS
SUMMARY 1
ZUSAMMENFASSUNG 3
1 INTRODUCTION: BIOMOLECULAR INTERACTION 5
1.1 RELEVANCE : 5
1.2 ANALYSIS 5
2 BACKGROUND: BIOMOLECULE THERMOPHORESIS ; 7
2.1 THERMOPHORESIS 7
2.2 ALL-OPTICAL MEASUREMENT 8
2.3 BINDING QUANTIFICATION 9
3 THERMOPHORESIS IN NANOLITER-DROPLETS 11
3.1 SAMPLE PREPARATION 11
3.2 INVERTED MICROSCOPIC SETUP 11
3.3 LOCAL HEATING IN EXPERIMENT AND SIMULATION 12
3.4 APTAMER QUANTIFICATION 14
3.5 DISCUSSION 16
4 THERMOPHORETIC PROTEIN STUDIES 19
4.1 PROTEIN-PROTEIN BINDING IN CELL LYSATE: TEM1-BLIP 19
4.2 PROTEIN-PEPTIDE BINDING WITH TWO PHASES: AMA1-RON2 20
4.3 PROTEIN-SMALL MOLECULE BINDING WITHOUT LABELING 23
4.3.1 GLUTAMATE RECEPTOR IGLUR2 23
4.3.2 MAP KINASE P38A 24
4.4 DISCUSSION 25
5 THERMOPHORETIC IMMUNOASSAYS 27
5.1 ANTIBODY CHARACTERIZATION 27
5.1.1 TOWARDS A THERAPEUTIC ANTIBODY FOR GRANULOMATOSIS WITH
POLYANGIITIS 27
5.1.2 MCPR3-7 AFFINITY AND SELECTIVITY 28
5.1.3 INTERFERENCE WITH PR3-ALPI COMPLEX FORMATION 28
5.2 QUANTITATIVE IN VITRO DIAGNOSTICS 30
5.2.1 AUTOANTIBODIES AS BIOMARKERS FOR DILATED CARDIOMYOPATHY 30
5.2.2 AFFINITY AND SPECIFICITY OF THE TRACER COR1 31
5.2.3 AUTOCOMPETITION ASSAY 32
5.2.4 QUANTIFICATION OF AFFINITY AND CONCENTRATION 32
5.3 DISCUSSION 35
6 CONCLUSION AND OUTLOOK 37
7 REFERENCES 39
8 DANKSAGUNG 45
9 ASSOCIATED PUBLICATIONS 47
|
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| author | Seidel, Susanne |
| author_facet | Seidel, Susanne |
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| language | English |
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| physical | 47, [57] S. Ill., graph. Darst. |
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| spellingShingle | Seidel, Susanne Nanoliter-droplet thermophoresis for biomedical applications |
| subject_GND | (DE-588)4113937-9 |
| title | Nanoliter-droplet thermophoresis for biomedical applications |
| title_auth | Nanoliter-droplet thermophoresis for biomedical applications |
| title_exact_search | Nanoliter-droplet thermophoresis for biomedical applications |
| title_full | Nanoliter-droplet thermophoresis for biomedical applications Susanne Seidel |
| title_fullStr | Nanoliter-droplet thermophoresis for biomedical applications Susanne Seidel |
| title_full_unstemmed | Nanoliter-droplet thermophoresis for biomedical applications Susanne Seidel |
| title_short | Nanoliter-droplet thermophoresis for biomedical applications |
| title_sort | nanoliter droplet thermophoresis for biomedical applications |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1056876654/34 https://nbn-resolving.org/urn:nbn:de:bvb:19-173039 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027526646&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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