MicroRNAs involved in melanoma cell cycle control and tumour progression:
Gespeichert in:
| Beteilige Person: | |
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| Format: | Hochschulschrift/Dissertation Elektronisch Software E-Book |
| Sprache: | Englisch |
| Veröffentlicht: |
2013
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| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027457216&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | XXX, 101 Bl. Ill., graph. Darst. 30 cm Beil. (1 S.) |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
TABLE OF CONTENTS I
TABLE OF FIGURES V
LIST OF TABLES VIII
ABBREVIATIONS IX
1 INTRODUCTION 1
1.1 CANCER BIOGENESIS 1
1.2 TUMOUR CELL INVASION AND METASTASIS 2
1.2.1 LOCAL INVASION 2
1.2.2 INTRAVASATION 3
1.2.3 SURVIVAL IN THE CIRCULATION 3
, 1.2.4 ARREST AT DISTANT SITES 4
1.2.5 MICROMETASTASIS FORMATION AND METASTATIC COLONIZATION 4
1.3 MELANOMA 5
1.3.1 HYPERPLASIA AND NEVUS FORMATION 5
1.3.2 DYSPLASTIC NEVUS 6
1.3.3 RADIAL GROWTH PHASE (RGP) 7
1.3.4 VERTICAL GROWTH PHASE (VGP) 7
1.3.5 METASTATIC MELANOMA 7
1.3.6 MELANOMA TARGETING 7
1.4 MICRORNAS 8
1.4.1 MIRNA BIOGENESIS 9
1.4.2 MIRNA TARGET PREDICTION 10
I
HTTP://D-NB.INFO/1048660400
1.4.3 MIRNAS IN MELANOMA 12
1.4.4 MIRNAS AS MELANOMA BIOMARKERS 14
1.5 CELL CYCLE 15
1.5.1 WEE
1 KINASE 16
1.6 AIMS OF THE STUDY 17
2 MATERIAL AND METHODS 19
2.1 MATERIALS 19
2.1.1 MICROSCOPES 19
2.1.2 CENTRIFUGES 19
2.1.3 OTHER EQUIPMENTS 19
2.1.4 GENERAL LABORATORY MATERIAL 20
2.1.5 CHEMICALS AND REAGENTS 21
2.1.6 KITS 23
2.1.7 MARKERS 24
2.1.8 HUMAN AND MURINE CELLS 24
2.1.9 PATIENT MATERIAL 24
2.2 BUFFERS AND MEDIA 25
2.2.1 CELL CULTURE MEDIA 25
2.2.2 PROTEIN BIOCHEMISTRY 26
2.2.3 MOLECULAR BIOLOGY 26
2.3 ANTIBODIES 27
2.3.1 PRIMARY ANTIBODIES 27
2.3.2 SECONDARY ANTIBODIES 27
2.4 SMALL INTERFERING RNAS (SIRNAS) AND MICRORNAS (MIRNAS) 28
II
2.5 PLASMIDS AND VIRAL PARTICLES 28
2.6 PRIMERS 29
2.7 SOFTWARE AND DATABASES 29
2.8 METHODS 30
2.8.1 MOLECULAR BIOLOGY 30
2.8.2 CELL CULTURE 32
2.8.3 FUNCTIONAL ASSAYS 34
2.8.4 PROTEIN BIOCHEMISTRY 36
2.8.5 HISTOPATHOLOGY 37
2.8.6 LENTIVIRAL GENE TRANSDUCTION 38
2.8.7 MOUSE EXPERIMENTS 39
2.8.8 IMMUNOPRECIPITATION 39
2.8.9 STATISTICAL ANALYSES 40
3 RESULTS 41
3.1 REGULATION OF CELL CYCLE CHECKPOINT KINASE WEE 1 BY MIR-195 41
3.1.1 WEE
1 IS DOWNREGULATED IN MELANOMA METASTASES 41
3.1.2 MIR-195 IS UPREGULATED IN MELANOMA METASTASES 42
3.1.3 MIR-195 REGULATES WEE1 EXPRESSION 43
3.1.4 MIR-195 ABROGATES GENOTOXIC STRESS-INDUCED G2-M ARREST 46
3.1.5 WEE1 OVEREXPRESSION REVERSES MIR-195-MEDIATED G2-M ABROGATION 50
3.1.6 MIR-195 ENHANCES PROLIFERATION AND MIGRATORY CAPACITY OF MELANOMA
CELLS 52
3.2 TAQMAN MIRNA EXPRESSION PROFILING IDENTIFIES MIR-638 AS AN ONCOMIR
IN
MELANOMA 54
3.2.1 MIRNA EXPRESSION PROFILING DISTINGUISHES MELANOMA STAGES 54
III
3.2.2 MIR-638 IS UPREGULATED DURING MELANOMA PROGRESSION 57
3.2.3 MIR-638 ENHANCES THE TUMOURIGENIC POTENTIAL OF MELANOMA CELLS 59
3.2.4 TP53INP2 AND BTG2 ARE MIR-638 TARGETS 62
3.2.5 KNOCKDOWN OF MIR-638 INDUCES P53-MEDIATED APOPTOSIS 68
3.2.6 DYNAMIN 2 (DNM2, DYN2) IS DISPENSABLE IN MELANOMA 71
3.2.7 REGULATORY FEEDBACK LOOP OF TFAP2A AND MIR-638 74
4 DISCUSSION 78
4.1 REGULATION OF CELL CYCLE CHECKPOINT KINASE WEE1 BY MIR-195 78
4.2 MIRNA EXPRESSION PROFILING IDENTIFIES MIR-638 AS AN ONCOMIR IN
MELANOMA 82
4.2.1 MIRNA EXPRESSION PROFILING IN MELANOMA 82
4.2.2 MIRNAS AS ONCOMIRS IN MELANOMA 84
4.2.3 IDENTIFICATION OF MIR-638 TARGETS 87
4.2.4 MIR-638 REGULATES P53-MEDIATED PATHWAYS 89
4.3 PERSPECTIVES 91
5 SUMMARY 93
6 ZUSAMMENFASSUNG 97
REFERENCES XV
ACKNOWLEDGMENTS XXV
CURRICULUM VITAE XXVI
PUBLICATIONS AND PRESENTATIONS XXVIII
ERKLARUNG TIBER DIE EIGENSTANDIGE ABFASSUNG DER ARBEIT XXX
IV
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| spellingShingle | Bhattacharya, Animesh 1982- MicroRNAs involved in melanoma cell cycle control and tumour progression |
| subject_GND | (DE-588)4113937-9 |
| title | MicroRNAs involved in melanoma cell cycle control and tumour progression |
| title_auth | MicroRNAs involved in melanoma cell cycle control and tumour progression |
| title_exact_search | MicroRNAs involved in melanoma cell cycle control and tumour progression |
| title_full | MicroRNAs involved in melanoma cell cycle control and tumour progression Animesh Bhattacharya |
| title_fullStr | MicroRNAs involved in melanoma cell cycle control and tumour progression Animesh Bhattacharya |
| title_full_unstemmed | MicroRNAs involved in melanoma cell cycle control and tumour progression Animesh Bhattacharya |
| title_short | MicroRNAs involved in melanoma cell cycle control and tumour progression |
| title_sort | micrornas involved in melanoma cell cycle control and tumour progression |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027457216&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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