The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2014
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| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027323781&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | XII, 169 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis |c von Katharina Fuchs |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
TABLE OF CONTENTS
ABSTRACT I
ZUSAMMENFASSUNG ILL
TABLE OF CONTENTS V
FIGURE INDEX VII
ABBREVIATIONS IX
INTRODUCTION 1
1 VASCULARIZATION UNDER PHYSIOLOGIC AND PATHOLOGIC CONDITIONS 1
1.1 VASCULOGENESIS 1
1.2 ANGIOGENESIS 1
1.3 REGULATION OF ANGIOGENESIS 4
1.4 TUMOR-INDUCED ANGIOGENESIS 6
1.5 MOLECULAR REGULATION OF TUMOR-INDUCED ANGIOGENESIS 8
2 CHEMOKINES 11
2.1 CXCR4/CXCL12 11
2.2 CXCL12/CXCR4 SIGNAL TRANSDUCTION 13
2.3 PHYSIOLOGICAL FUNCTIONS OF THE CXCR4/CXCL12 AXIS 14
2.4 CXCR4 IN ANGIOGENESIS 16
2.5 CXCR4 AND CXCL12 IN PATHOLOGICAL PROCESSES 17
3 CD44 21
3.1 THE STRUCTURE OF CD44 21
3.2 PHYSIOLOGICAL FUNCTIONS OF CD44 25
3.3 CD44 IN ANGIOGENESIS 29
3.4 CD44 IN INFLAMMATION, AUTOIMMUNE DISEASE AND CANCER 30
3.5 THE MOLECULAR FUNCTION OF CD44 31
4 HYALURONAN 35
4.1 BIOLOGICAL SYNTHESIS AND DEGRADATION OF HYALURONAN 36
4.2 PHYSIOLOGICAL FUNCTIONS OF HYALURONAN 38
4.3 SMALL HYALURONAN FRAGMENTS (SHA) 39
4.4 ROLE OF HYALURONAN IN CANCER 40
4.5 HA IN ANGIOGENESIS 41
4.6 HA-BINDING TO CD44 42
5 EXTRAVASATION 45
AIM OF THE WORK 49
MATERIALS AND METHODS 51
1 MATERIALS 51
1.1 CHEMICALS AND CELL CULTURE EQUIPMENT 51
1.2 HARDWARE AND CONSUMABLES 53
1.3 ANTIBODIES, OLIGONUCLEOTIDES AND PRIMER 55
1.4 GROWTH FACTORS AND CELL CULTURE REAGENTS 56
V
HTTP://D-NB.INFO/1050296028
TABLE OF CONTENTS
1.5 CELL LINES AND ANIMALS 57
2 METHODS 59
2.1 CELL CULTURE 59
2.2 PROTEIN METHODS 61
2.3 DNA-METHODS 64
2.4 IN-VITRO EXPERIMENTS 64
2.5 EXPERIMENTS WITH ANIMALS 67
2.6 IMMUNOHISTOLOGICAL ANALYSIS 70
2.7 MICROFLUIDIC SHEAR STRESS EXPERIMENT 72
EXPERIMENTS AND RESULTS 75
1 PART I: HYALURONAN AND CD44 MODULATE CXCL12-INDUCED ACTION 75
1.1 HYALURONAN MODULATES CXCR4 ACTIVATION 75
1.2 HYALURONAN IS A MODULATOR OF CXCL12-INDUCED MIGRATION 78
1.3 CXCL12-INDUCED ANGIOGENESIS IS MODULATED BY HYALURONAN 82
1.4 CD44 REGULATES THE EFFECT OF HA ON CXCL12-INDUCED SIGNALING,
MIGRATION AND
SPROUTING 84
1.5 CD44 AND CXCR4 FORM A HA-DEPENDENT COMPLEX 89
1.6 HYALURONAN AND CD44 ARE KEY MODULATORS IN CXCL12-INDUCED
ANGIOGENESIS IN
VIVO 91
2 VESSEL-SPECIFIC DISRUPTION OF CD44 AFFECTS IN VIVO VASCULARIZATION 94
2.1 EFFICIENT INACTIVATION OF THE CD44 GENE IN THE VESSELS 95
2.2 CXCL12-INDUCED AORTA SPROUTING IS REDUCED IN CD44
IAEC
MICE 96
2.3 CXCL12-INDUCED VASCULARIZATION OF MATRIGEL PLUGS IS REDUCED IN CD44
IAEC
MICE98
2.4 THE ROLE OF CD44 IN IN VIVO WOUND HEALING 101
3 PART II: ROLLING OF HEPG2ISO CELLS ON HA IS DEPENDENT ON CD44 107
3.1 ONLY LIVER CARCINOMA CELLS EXPRESSING CD44 ROLL ON HA-COATED
SURFACES 108
3.2 THE INFLUENCE OF CD44-KNOCKDOWN ON ROLLING OF HEPG2ISO CELLS 110
3.3 A CD44 ANTIBODY PREVENTS ROLLING OF HEPG2ISO CELLS ON HA 114
3.4 TREATMENT OF CELLS WITH SHA IS ABLE TO PREVENT ROLLING OF HEPG2ISO
CELLS 117
DISCUSSION 121
1 PART I: HYALURONAN AND CD44 MODULATE CXCL12-INDUCED ACTION 121
2 PART II: ROLLING OF HEPG2ISO CELLS ON HA IS DEPENDENT ON CD44 131
BIBLIOGRAPHY 137
ACKNOWLEDGMENTS 165
PUBLICATION 167
CURRICULUM VITAE 169
VI
|
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| author | Fuchs, Katharina 1985- |
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| dewey-ones | 610 - Medicine and health 570 - Biology |
| dewey-raw | 610 570 |
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| dewey-tens | 610 - Medicine and health 570 - Biology |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| indexdate | 2024-12-20T16:56:51Z |
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| language | English |
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| physical | XII, 169 S. Ill., graph. Darst. |
| publishDate | 2014 |
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| spellingShingle | Fuchs, Katharina 1985- The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis |
| subject_GND | (DE-588)4113937-9 |
| title | The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis |
| title_auth | The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis |
| title_exact_search | The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis |
| title_full | The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis von Katharina Fuchs |
| title_fullStr | The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis von Katharina Fuchs |
| title_full_unstemmed | The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis von Katharina Fuchs |
| title_short | The role of the extracellular matrix component hyaluronan and its receptor CD44 in chemokine-induced angiogenesis and metastatis |
| title_sort | the role of the extracellular matrix component hyaluronan and its receptor cd44 in chemokine induced angiogenesis and metastatis |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027323781&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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