Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2013
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| Schlagwörter: | |
| Links: | http://mediatum.ub.tum.de/node?id=1135819 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20131216-1135819-0-0 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027034098&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 150 S. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Büttner, Barbara Elisabeth |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs |c Barbara Elisabeth Büttner |
| 264 | 1 | |c 2013 | |
| 300 | |a 150 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Techn. Univ., Diss., 2013 | ||
| 546 | |a Zsfassung in dt. Sprache | ||
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| 856 | 4 | 1 | |u http://mediatum.ub.tum.de/node?id=1135819 |x Verlag |z kostenfrei |3 Volltext |
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| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027034098&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-027034098 | |
Datensatz im Suchindex
| DE-BY-TUM_call_number | 0001 DM 31795 1001 Diss.4 4652 |
|---|---|
| DE-BY-TUM_katkey | 1981604 |
| DE-BY-TUM_location | Mag |
| DE-BY-TUM_media_number | TEMP3229353 040050896867 |
| _version_ | 1821933652278247425 |
| adam_text | CONTENTS
LIST OF FIGURES 13
LIST OF TABLES 15
LIST OF PUBLICATIONS 17
LIST OF ABBREVIATIONS 19
1. INTRODUCTION 23
1.1. FOLATE 23
1.2. HUMAN FOLATE METABOLISM 25
1.2.1. FOLATE ABSORPTION 25
1.2.2. FOLATE DISTRIBUTION AND PHYSIOLOGICAL FUNCTIONS 26
1.2.3. FOLATE TURNOVER AND EXCRETION 28
1.3. FOLATE BIOAVAILABILITY AND INTAKE 28
1.3.1. BIOAVAILABILITY 28
1.3.2. METHODS TO DETERMINE BIOAVAILABILITY 30
1.3.3. FOLATE STATUS AND INTAKE 31
1.4. DETERMINATION OF FOLATE IN DIFFERENT SAMPLES 32
1.4.1. SAMPLE PREPARATION FOR CHROMATOGRAPHIC ASSAYS 32
1.4.2. LIQUID CHROMATOGRAPHY SEPARATION 33
1.4.3. DETECTION 34
1.4.4. STABLE ISOTOPE DILUTION ASSAY 36
1.5. FOLATE IN BREAST MILK 38
1.5.1. FOLATE SUPPLY OF INFANTS 38
1.5.2. SIGNIFICANCE OF BREAST MILK CONSUMPTION FOR INFANTS 39
HTTP://D-NB.INFO/1048484785
1.5.3. BANKING OF BREAST MILK 41
1.5.4. EFFECT, OF HEAT TREATMENT ON BREAST MILK FOLATE CONTENT 43
2. OBJECTIVES 45
3. MATERIALS AND METHODS 47
3.1. CHEMICALS AND MATERIALS 47
3.1.1. TECHUISCHE UNIVERSITAT MIINCHEN 47
3.1.2. SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES 48
3.2. METHODS 50
3.2.1. FOLATE EXTRACTION FROM PLASMA, ILEOSTOMAL EFFLUENT AND URINE 50
3.2.2. FOLATE QUANTIFICATION IN PLASMA, ILEOSTOMAL EFFLUENT AND URINE .
53
3.2.3. FOLATE EXTRACTION FROM BREAST MILK 59
3.2.4. FOLATE QUANTIFICATION IN BREAST MILK 61
3.3. INSTRUMENTAL DATA 63
3.3.1. LIQUID CHROINATOGRAPHY-TANDEM MASS SPECTROMETRY SYSTEMS . . 63
3.3.2. HIGH PERFORMANCE LIQUID CHROINATOGRAPHY-ULTRAVIOLETT/FLUORESCENCE
DETECTION SYSTEM 63
3.3.3. PREPARATIVE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY SYSTEM . 65
3.4. STUDY DESIGN 65
3.4.1. HUMAN STABLE-ISOTOPE AREA UNDER THE CURVE/ILEOSTOMY MODEL . 65
3.4.2. FOLATE STABILITY DURING HEAT TREATMENT OF BREAST MILK 66
4. RESULTS AND DISCUSSION 69
4.1. SYNTHESIS OF FOLATE STANDARDS 69
4.1.1. RELATIVE ABUNDANCE P 69
4.1.2. SYNTHESIS PATHWAJ S AND YIELDS 70
4.1.3. STUDIES BY MASS SPECTROMETRY AND NUCLEAR MAGNETIC RESONANCE
SPECTROSCOPY 70
4.2. DEVELOPMENT OF STABLE ISOTOPE DILUTION ASSAYS FOR ANALYSIS OF HUMAN
SAMPLES 72
4.2.1. PRECIPITATION OF PLASMA PROTEIN 72
4.2.2. PREPARATION OF ILEOSTOMAL EFFLUENT SAMPLES 73
4.2.3. DETERMINATION OF FOLATE CATABOLITES ILL URINE 76
4.2.4. LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY ANALYSIS . 78
4.3. BIOAVAILABILITY OF FOLATE FROM WHOLEMEAL BREAD 80
4.3.1. FOLATE CONTENT IN PLASMA AND ILEOSTOMAL EFFLUENT 80
4.3.2. DETERMINATION OF FOLATE BIOAVAILABILITY BY A HUMAN STABLE-
ISOTOPE AREA UNDER THE CURVE/ILEOSTOMY MODEL 82
4.4. FOLATE STABILITY DURING HEAT TREATMENT OF BREAST MILK 83
4.4.1. PREPARATION OF BREAST MILK SAMPLES 84
4.4.2. ADJUSTMENT OF THE HEAT TREATMENT PROCEDURES 8G
4.4.3. HEAT TREATMENT OF BREAST MILK 88
5. CONCLUDING REMARKS AND FUTURE PERSPECTIVES 91
6. REFERENCES 93
A. SYNTHESIS OF FOLATE STANDARDS 109
A.L. UNLABELLED AP-ABG 110
A.2. [
2
H
4
]-P-ABG AND [
2
H
4
]-AP-ABG 112
A.2.1. DEUTERATION OF P-ABA 112
A.2.2. PROTECTION OF PH
4
]-P-ABA 113
A.2.3. COUPLING OF PHJJ-P-ABA AND L-GLUTAMIC ACID 114
A.2.4. CLEAVAGE OF PROTECTION GROUPS AND MASS SPECTRAL ANALYSIS OF
THE FINAL SYNTHESIS PRODUCTS 115
B. DEVELOPMENT OF STABLE ISOTOPE DILUTION ASSAYS FOR ANALYSIS OF HUMAN
SAMPLES 118
B.L. PRECIPITATION OF PLASMA PROTEIN 119
B.2. PREPARATION OF ILEOSTOMAL EFFLUENT SAMPLES 121
B.3. DETERMINATION OF FOLATE CATABOLITES IN URINE 130
C. BIOAVAILABILITY OF FOLATE FROM WHOLEMEAL BREAD 132
C.L. PLASMA RESPONSE 133
C.2. FOLATE IN ILEOSTOMAL EFFLUENT 134
D. FOLATE STABILITY DURING HEAT TREATMENT OF BREAST MILK 137
D.L. PREPARATION OF BREAST MILK SAMPLES 138
D.2. ADJUSTMENT OF THE HEAT TREATMENT PROCEDURES 143
D.3. HEAT TREATMENT OF BREAST MILK 144
E. PUBLICATIONS 146
E.L. PAPER I - SUMMARY 147
E.2. PAPER II - SUMMARY 148
E.3. PAPER III - SUMMARY 149
E.4. PAPER IV - SUMMARY 150
ACKNOWLEDGEMENTS 151
CURRICULUM VITAE 155
|
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| spellingShingle | Büttner, Barbara Elisabeth Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs |
| subject_GND | (DE-588)4113937-9 |
| title | Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs |
| title_auth | Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs |
| title_exact_search | Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs |
| title_full | Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs Barbara Elisabeth Büttner |
| title_fullStr | Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs Barbara Elisabeth Büttner |
| title_full_unstemmed | Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs Barbara Elisabeth Büttner |
| title_short | Analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope, ileostomy and breast milk designs |
| title_sort | analysis of intact folate and folate catabolites in bioavailability and stability studies using stable isotope ileostomy and breast milk designs |
| topic_facet | Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1135819 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20131216-1135819-0-0 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027034098&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT buttnerbarbaraelisabeth analysisofintactfolateandfolatecatabolitesinbioavailabilityandstabilitystudiesusingstableisotopeileostomyandbreastmilkdesigns |
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