Cone-beam x-ray phase contrast tomography of biological samples: optimization of contrast, resolution and field of view
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Deutsch |
| Veröffentlicht: |
Göttingen
Univ.-Verl.
2013
|
| Schriftenreihe: | Göttingen Series in x-ray physics
13 |
| Schlagwörter: | |
| Links: | http://digitool.hbz-nrw.de:1801/webclient/DeliveryManager?pid=5379222&custom_att_2=simple_viewer http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026918800&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | IV, 205 S. zahlr. Ill., graph. Darst. |
| ISBN: | 9783863951344 |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENTS
INTRODUCTION 1
1 TWO-DIMENSIONAL X-RAY PROPAGATION IMAGING 5
1.1 PROPAGATION OF X-RAYS IN FREE SPACE 6
1.1.1 WAVE EQUATIONS IN FREE SPACE 6
1.1.2 ANGULAR SPECTRUM APPROACH . : 7
1.1.3 PARAXIAL HELMHOTZ EQUATION 9
1.1.4 FRESNEL DIFFRACTION 10
1.1.5 FRAUNHOFER DIFFRACTION 12
1.1.6 FRESNEL SCALING THEOREM 12
1.1.7 NUMERICAL IMPLEMENTATION OF FRESNEL DIFFRACTION 14
1.2 X-RAY INTERACTION WITH MATTER 15
1.2.1 WAVE EQUATIONS IN THE PRESENCE OF MATTER 15
1.2.2 REFRACTIVE INDEX FOX X-RAYS 16
1.2.3 THE PROJECTION APPROXIMATION 17
1.2.4 PHASE SHIFT AND ABSORPTION WITHIN OBJECTS 19
1.3 CONTRAST FORMATION AND IMAGING REGIMES 20
1.3.1 CONTRAST TRANSFER FUNCTION 21
1.3.2 TRANSPORT-OF-INTENSITY EQUATION 22
1.3.3 IMAGING REGIMES 23
1.4 EFFECTIVE NEAR FIELD AND EMPTY BEAM DIVISION 25
1.5 COHERENCE 26
1.6 RADIATION DAMAGE AND DOSE 30
2 SOLVING THE PHASE PROBLEM 31
2.1 HOLOGRAPHIC RECONSTRUCTION 32
2.2 TRANSPORT-OF-INTENSITY EQUATION 35
2.2.1 PURE PHASE OBJECT 37
2.2.2 TREATMENT OF ABSORPTION 38
2.3 CONTRAST TRANSFER FUNCTION 41
2.3.1 PURE PHASE OBJECT 41
2.3.2 TREATMENT OF ABSORPTION 44
2.4 ITERATIVE ALGORITHMS 44
2.4.1 GERCHBERG-SAXTON-FIENUP TYPE ALGORITHMS 46
2.4.1.1 GERCHBERG-SAXTON ALGORITHM 46
HTTP://D-NB.INFO/1041945728
II CONTENTS
2.4.1.2 ERROR REDUCTION ALGORITHM 47
2.4.1.3 HYBRID INPUT-OUTPUT ALGORITHM 48
2.4.2 NEWTON METHODS 50
2.5 HYBRID APPROACHES 51
3 THREE-DIMENSIONAL X-RAY PROPAGATION IMAGING 53
3.1 FOURIER SLICE THEOREM 55
3.2 FILTERED BACKPROJECTION 56
3.3 CONE-BEAM TOMOGRAPHY 57
3.4 RECONSTRUCTION AND VISUALIZATION SOFTWARE 58
4 IMPLEMENTATION OF X-RAY PROPAGATION IMAGING 59
4.1 X-RAY DETECTORS 59
4.2 LABORATORY SETUP JULIA 60
4.3 GINIX AT BEAMLINE P10 OF PETRA III 62
4.3.1 COHERENCE BEAMLINE P10 62
4.3.2 GINIX 63
4.3.3 X-RAY WAVEGUIDES 66
4.3.4 ORIGINAL WAVEGUIDE CONFIGURATION 69
4.3.5 OPTIMIZED WAVEGUIDE CONFIGURATION 70
4.3.6 KB CONFIGURATION 72
4.4 BEAMLINE ID22NI OF THE ESRF 76
4.5 DISTANCE CALIBRATION 79
4.5.1 DISTANCE CALIBRATION WITH PERIODIC STRUCTURE 79
4.5.2 DISTANCE CALIBRATION USING HOLOGRAPHIC RECONSTRUCTION ... 81
5 THREE-DIMENSIONAL IMAGING OF RODENT COCHLEA 83
5.1 CHALLENGES OF COCHLEAR IMAGING 84
5.2 EXPERIMENT 85
5.2.1 EXPERIMENTAL SETUP 85
5.2.2 SAMPLE PREPARATION AND MOUNTING 86
5.2.3 ALIGNMENT OF ROTATION AXIS 89
5.3 DATA ANALYSIS 91
5.3.1 PHASE AND TOMOGRAPHIC RECONSTRUCTION 91
5.3.2 THRESHOLD BASED SEGMENTATION OF BONE 93
5.4 RESULTS 94
5.4.1 SOLID TARGET SOURCE 94
5.4.2 LIQUID METAL JET SOURCE 98
5.4.3 OPTIMIZING SPATIAL RESOLUTION 99
5.4.4 SPACE ESTIMATION FOR INTRA-COCHLEAR IMPLANTS 105
5.5 SUMMARY 106
CONTENTS III
6 THREE-DIMENSIONAL IMAGING OF MOUSE NERVES 107
6.1 EXPERIMENTS 108
6.1.1 EXPERIMENTAL SETUP I 108
6.1.2 EXPERIMENTAL SETUP II 109
6.1.3 EXPERIMENTAL SETUP III 109
6.1.4 SAMPLE PREPARATION AND MOUNTING 110
6.1.4.1 IMMERSION FIXATION 110
6.1.4.2 MOUNTING OF IMMERSION FIXATED SAMPLES 110
6.1.4.3 EPON-EMBEDDING OF IMMERSION FIXATED SAMPLES . 112
6.1.4.4 HIGH PRESSURE FREEZING AND EPON-EMBEDDING . . . 113
6.1.4.5 LIGHT- AND ELECTRON MICROSCOPY 113
6.2 DATA ANALYSIS 114
6.2.1 TREATMENT OF RAW DATA 114
6.2.2 PHASE RETRIEVAL 117
6.2.3 SINGLE-DISTANCE PHASE RETRIEVAL 117
6.2.4 MULTI-DISTANCE PHASE RETRIEVAL 120
6.2.5 TOMOGRAPHIC RECONSTRUCTION 122
6.3 RESULTS 122
6.3.1 UNSTAINED NERVES 122
6.3.2 OSMIUM TETROXIDE STAIN 123
6.3.3 HIGHER SPATIAL RESOLUTION FOR IMAGING OF OPTICAL NERVES . . . 127
6.3.4 HIGH RESOLUTION IMAGING USING HIGH PRESSURE FREEZING .... 129
6.4 SUMMARY 134
7 THREE-DIMENSIONAL DENSITY CONTRAST OF DEINOCOCCUS RADIODURANS 135
7.1 EXPERIMENT 136
7.1.1 EXPERIMENTAL SETUP 136
7.1.2 SAMPLE PREPARATION AND MOUNTING 137
7.2 DATA ANALYSIS 138
7.2.1 TREATMENT OF RAW DATA 138
7.2.2 PHASE RETRIEVAL AND AUTOMATIC SUPPORT DETERMINATION .... 141
7.3 RESULTS 141
7.3.1 2D PHASE RECONSTRUCTION 141
7.3.2 THREE-DIMENSIONAL MASS DENSITY 144
7.3.3 DOSE AND RESOLUTION 144
7.4 SUMMARY 146
8 TOWARDS HIGHER RESOLUTION 147
8.1 IMAGING OF DICTYOSTELIUM DISCOIDEUM CELLS 147
8.1.1 SAMPLE PREPARATION 147
8.1.2 EXPERIMENTAL SETUP 147
8.1.3 EMPTY BEAM AND DRIFT CORRECTION 149
8.1.4 ASTIGMATISM 151
8.1.5 PHASE RECONSTRUCTION 152
8.1.6 DOSE AND RESOLUTION 154
IV
CONTENTS
8.2 OPTIMIZED WAVEGUIDE CONFIGURATION 155
8.2.1 IMAGING OF A TEST STRUCTURE AT 13.8 KEV 155
8.2.1.1 EXPERIMENTAL SETUP 155
8.2.1.2 RAW DATA TREATMENT 156
8.2.1.3 PHASE RECONSTRUCTION 158
8.2.1.4 RESOLUTION 161
8.2.2 IMAGING OF DEINOCOCCUS RADIODURANS AT 13.8 KEV 161
8.2.2.1 EXPERIMENTAL SETUP AND RAW DATA TREATMENT . . . 162
8.2.2.2 PHASE RECONSTRUCTION 162
8.2.2.3 DOSE AND RESOLUTION 164
8.2.3 IMAGING OF DEINOCOCCUS RADIODURANS AT 7.9 KEV 166
8.2.3.1 EXPERIMENTAL SETUP AND RAW DATA TREATMENT . . . 166
8.2.3.2 PHASE RECONSTRUCTION 168
8.2.3.3 RESOLUTION AND DOSE 170
8.3 SUMMARY 171
CONCLUSION 173
APPENDIX 175
A.L FOURIER ANALYSIS 175
A.1.1 FOURIER TRANSFORM 175
A.1.2 PROPERTIES OF THE FOURIER TRANSFORM 175
A.1.3 FOURIER TRANSFORM OF THE DIRAC DELTA FUNCTION 177
A.2 FREE SPACE PROPAGATION OF CONJUGATE OBJECTS 177
A.3 SPATIAL RESOLUTION 178
BIBLIOGRAPHY 199
LIST OF PUBLICATIONS 201
DANKSAGUNG 203
CURRICULUM VITAE
207
|
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| id | DE-604.BV041472689 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T16:48:11Z |
| institution | BVB |
| isbn | 9783863951344 |
| language | German |
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| physical | IV, 205 S. zahlr. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Univ.-Verl. |
| record_format | marc |
| series | Göttingen Series in x-ray physics |
| series2 | Göttingen Series in x-ray physics |
| spellingShingle | Bartels, Matthias 1985- Cone-beam x-ray phase contrast tomography of biological samples optimization of contrast, resolution and field of view Göttingen Series in x-ray physics Computertomografie (DE-588)4113240-3 gnd Biologisches Material (DE-588)4145633-6 gnd |
| subject_GND | (DE-588)4113240-3 (DE-588)4145633-6 (DE-588)4113937-9 |
| title | Cone-beam x-ray phase contrast tomography of biological samples optimization of contrast, resolution and field of view |
| title_auth | Cone-beam x-ray phase contrast tomography of biological samples optimization of contrast, resolution and field of view |
| title_exact_search | Cone-beam x-ray phase contrast tomography of biological samples optimization of contrast, resolution and field of view |
| title_full | Cone-beam x-ray phase contrast tomography of biological samples optimization of contrast, resolution and field of view Matthias Bartels |
| title_fullStr | Cone-beam x-ray phase contrast tomography of biological samples optimization of contrast, resolution and field of view Matthias Bartels |
| title_full_unstemmed | Cone-beam x-ray phase contrast tomography of biological samples optimization of contrast, resolution and field of view Matthias Bartels |
| title_short | Cone-beam x-ray phase contrast tomography of biological samples |
| title_sort | cone beam x ray phase contrast tomography of biological samples optimization of contrast resolution and field of view |
| title_sub | optimization of contrast, resolution and field of view |
| topic | Computertomografie (DE-588)4113240-3 gnd Biologisches Material (DE-588)4145633-6 gnd |
| topic_facet | Computertomografie Biologisches Material Hochschulschrift |
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