Verfügbarkeit: In vivo functional mapping of single synapses in the vibrissal cortex of the mouse

In vivo functional mapping of single synapses in the vibrissal cortex of the mouse:

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Bibliographische Detailangaben
Beteilige Person:	Varga, Zsuzsanna (VerfasserIn)
Format:	Hochschulschrift/Dissertation Buch
Sprache:	Englisch
Veröffentlicht:	2012
Schlagwörter:	Hochschulschrift
Links:	http://mediatum.ub.tum.de/node?id=1113624 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20121026-1113624-1-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026882468&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Umfang:	106 Bl. Ill., graph. Darst.

Internformat

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Datensatz im Suchindex

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adam_text	TABLE OF CONTENTS 1. INTRODUCTION 7 1.1. THE ANATOMY OF THE MOUSE WHISKER SYSTEM 9 1.1.1. PERIPHERAL SENSORY ORGANS: VIBRISSAE 9 1.1.2. SENSORY PATHWAYS CONNECTING VIBRISSAE TO 51 10 1.1.3. THE MOUSE WSL 11 1.1.3.1. BARRELS 11 1.1.3.2. CONNECTIONS OF MOUSE WSL 12 1.1.3.3. MICROCIRCUITRY OF MOUSE SI 14 1.1.3.4. MORPHOLOGY OF L2 AND L4 EXCITATORY NEURONS IN MOUSE SI 15 1.2. THE FUNCTION OF MOUSE SI 17 1.2.1. RECEPTIVE FIELD PROPERTIES OFL2 AND L4 NEURONS IN MOUSE WSL 17 1.2.2. TOUCH REPRESENTATION IN THE MOUSE SI 18 1.3. METHODS FOR STUDYING SYNAPTIC CONNECTIONS IN VITRO AND IN VIVO 22 1.3.1. ANATOMICAL RECONSTRUCTIONS 22 1.3.2. ELECTROPHYSIOLOGICAL PAIR RECORDINGS 24 1.3.3. PHOTOSTIMULATION 24 1.3.3.1. OPTOGENETICS 24 1.3.3.2. GLUTAMATE UNCAGING 25 1.3.4. TWO-PHOTON CALCIUM IMAGING 25 1.4. DENDRITES AND SPINES 27 2. AIMS 30 3. MATERIALS AND METHODS 31 3.1. ANIMALS AND SURGICAL PROCEDURES 31 3.1.1.12 EXPERIMENTS 31 3.1.2. L4 EXPERIMENTS 32 3.2. WHISKER STIMULATION PROTOCOL 32 3.2.1. L2 EXPERIMENTS 32 3.2.2. L4 EXPERIMENTS 33 3.3. INTRINSIC SIGNAL OPTICAL IMAGING 33 3.3.1.12 EXPERIMENTS 33 3.3.2. L4 EXPERIMENTS 34 3.4. FILLING THE NEURONS WITH CALCIUM-SENSITIVE DYE 34 3.4.1.12 EXPERIMENTS - TWO-PHOTON TARGETED WHOLE-CELL CONFIGURATION 34 3.4.2. L4 EXPERIMENTS - TWO-PHOTON TARGETED SINGLE CELL ELECTROPORATION 34 HTTP://D-NB.INFO/1045000728 3.5. ELECTROPHYSIOLOGICAL RECORDINGS 35 3.5.1.12 EXPERIMENTS - WHOLE-CELL RECORDINGS 35 3.5.2. L4 EXPERIMENTS - LOOSE CELL-ATTACHED RECORDINGS 35 3.6. SYNCHRONIZATION OF ELECTRICAL RECORDINGS WITH CALCIUM IMAGING AND STIMULATION TIMING 36 3.6.1.12 EXPERIMENTS - PRE-SET MODE 36 3.6.2. L4 EXPERIMENTS - ARBITRARY EVENT MODE 36 3.7. TWO-PHOTON CALCIUM IMAGING 36 3.7.1.12 EXPERIMENTS 36 3.7.2. L4 EXPERIMENTS 38 3.8. DRUG APPLICATION 38 3.8.1. L4 EXPERIMENTS 38 3.9. POST-HOC PROCEDURES 38 3.9.1. L2 EXPERIMENTS 38 3.10. DATA ANALYSIS 39 3.10.1.12 EXPERIMENTS 39 3.10.1.1. RECONSTRUCTION OF DENDRITIC TREES 39 3.10.1.2. CALCULATION OF WHISKER-EVOKED SUBTHRESHOLD MEMBRANE POTENTIAL DEPOLARIZATION 39 3.10.1.3. SPATIAL-TEMPORAL FILTERING OF CALCIUM IMAGING DATA AND TRANSFORMATION INTO AF/F IMAGE SEQUENCES 39 3.10.1.4. CALCULATION OF GLOBAL DENDRITIC CALCIUM SIGNALS 40 3.10.1.5. DETECTION AND CATEGORIZATION OF DENDRITIC HOTSPOT SITES 40 3.10.1.6. CALCULATION OF CALCIUM SIGNALS OF INDIVIDUAL SPINES 41 3.10.2. L4 EXPERIMENTS 41 3.10.2.1. RECONSTRUCTION OF DENDRITIC TREES 41 3.10.2.2. AUTOMATIC RECOGNITION OF STIMULATION EVOKED RESPONSES 41 3.10.2.3. CALCULATION OF RESPONSE ONSET TIME 41 3.10.2.4. CALCULATION OF SUBTHRESHOLD RESPONSE PROBABILITIES 42 3.10.2.5. CLASSIFICATION OF SHORT LATENCY SPINES 42 4. RESULTS 43 4.1. L2 NEURONS 43 4.1.1. IMAGING DENDRITES OFL2 NEURONS IN THE MOUSE WSL 43 4.1.2. DISTANCE DEPENDENCE OF ELECTRICAL AND GLOBAL CALCIUM SIGNALS 46 4.1.3. DISTRIBUTION OF INPUTS TO L2 NEURONS 48 4.1.4. DISTANCE DEPENDENCE OF THE NUMBER OF INPUTS TO 12 NEURONS 52 4.1.5. SPINE IMAGING REVEALS SHARED SPINES 53 4.1.6. SUMMARY OF THE RESULTS OF L2 EXPERIMENTS 57 5 4.2. L4 NEURONS 59 4.2.1. TWO-PHOTON CALCIUM IMAGING OF SPINES IN L4 NEURONS IN THE MOUSE WSL 59 4.2.2. CHARACTERIZATION OF WHISKER STIMULATION EVOKED INPUTS TO L4 61 4.2.3. SPATIAL DISTRIBUTION OF SHORT LATENCY SPINES 68 4.2.4. SUMMARY OF THE RESULTS OF L4 EXPERIMENTS 71 5. DISCUSSION 72 5.1. TECHNICAL ADVANCES AND ASPECTS 73 5.1.1. COMMON COORDINATE SYSTEM OF INTRINSIC SIGNAL OPTICAL IMAGING AND TWO- PHOTON CALCIUM IMAGING 73 5.1.2. DYE FILLING APPROACHES 74 5.1.3. WHISKER STIMULATION PROTOCOLS 75 5.1.4. MORPHOLOGY RECONSTRUCTIONS FROM Z-STACKS 75 5.1.5. SPATIAL RESOLUTION OF HOTSPOTS IN DENDRITIC IMAGING 76 5.1.6. TEMPORAL RESOLUTION TO IDENTIFY EARLY RESPONSES 76 5.2. SIMILAR PROPERTIES OF INPUTS REACHING L2 AND L4 NEURONS 77 5.2.1. CALCIUM TRANSIENTS LOCALIZED TO SPINES 77 5.2.2. DISTRIBUTED INPUTS 77 5.2.3. TEMPORAL VARIABILITY OF INPUT ACTIVATION 78 5.3. SPECIFIC PROPERTIES OF INPUTS REACHING L2 NEURONS 79 5.3.1. SHARED SPINES 79 5.3.2. DISTANCE DEPENDENCE OF SW-SPECIFIC AND SHARED SPINE 80 5.4. SPECIFIC PROPERTIES OF INPUTS REACHING L4 NEURONS 81 5.4.1. SHORT LATENCY INPUTS 81 5.4.2. SPATIAL DISTRIBUTION OF SHORT LATENCY INPUTS TO SPINY STELLATES AND STAR PYRAMIDS 81 5.5. MODEL CIRCUITRY 82 6. SUMMARY 84 7. ABBREVIATIONS 85 8. REFERENCES 86 9. ACKNOWLEDGEMENTS 105 10. PUBLICATIONS CONTAINING THESE RESULTS 106 6
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spellingShingle	Varga, Zsuzsanna In vivo functional mapping of single synapses in the vibrissal cortex of the mouse
subject_GND	(DE-588)4113937-9
title	In vivo functional mapping of single synapses in the vibrissal cortex of the mouse
title_auth	In vivo functional mapping of single synapses in the vibrissal cortex of the mouse
title_exact_search	In vivo functional mapping of single synapses in the vibrissal cortex of the mouse
title_full	In vivo functional mapping of single synapses in the vibrissal cortex of the mouse Zsuzsanna Varga
title_fullStr	In vivo functional mapping of single synapses in the vibrissal cortex of the mouse Zsuzsanna Varga
title_full_unstemmed	In vivo functional mapping of single synapses in the vibrissal cortex of the mouse Zsuzsanna Varga
title_short	In vivo functional mapping of single synapses in the vibrissal cortex of the mouse
title_sort	in vivo functional mapping of single synapses in the vibrissal cortex of the mouse
topic_facet	Hochschulschrift
url	http://mediatum.ub.tum.de/node?id=1113624 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20121026-1113624-1-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026882468&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT vargazsuzsanna invivofunctionalmappingofsinglesynapsesinthevibrissalcortexofthemouse

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