A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2013
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| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026824832&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 102 S. Ill., graph. Darst. |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
LIST OF ABBREVIATIONS 7
SUMMARY 9
ZUSAMMENFASSUNG 11
1 GENERAL INTRODUCTION 13
1.1 MYB TRANSCRIPTION FACTORS IN ARABIDOPSIS 13
1.1.1 FUNCTIONAL DOMAIN OF MYB TRANSCRIPTION FACTORS 13
1.1.2 DIVERSIFIED FUNCTIONS OF MYB TRANSCRIPTION FACTORS IN ARABIDOPSIS
15
1.2 ABA BIOSYNTHESIS 19
1.2.1 EARLY STEPS OF THE ABA BIOSYNTHESIS: CAROTENOID BIOSYNTHESIS 20
1.2.2 LATER STEPS IN THE ABA BIOSYNTHETIC PATHWAY 21
1.3 ABA CATABOLISM 24
1.3.1 ABA HYDROXYLATION 24
1.3.2 ABA CONJUGATION 25
1.4 ABA TRANSPORT 26
1.5 REGULATION OF ABA SYNTHESIS AND CATABOLISM 26
1.5.1 REGULATORY FACTORS IN ABA SYNTHESIS AND CATABOLISM 26
1.5.2 ABA METABOLISM IN RESPONSE TO DROUGHT AND SALT STRESS RESPONSES 27
1.5.3 MOLECULAR MECHANISM IN THE REGULATION OF ABA METABOLISM 29
1.6 ABA SIGNAL PERCEPTION AND TRANSDUCTION 31
1.7 ABA-MEDIATED TRANSCRIPTIONAL REGULATION IN RESPONSE TO STRESS 32
1.8 INTRODUCTION SUMMARY 34
1.9 AIM OF PROJECT 35
2 MATERIAL AND METHODS 36
2.1 CHEMICALS 36
2.2 SYNTHETIC OLIGONUCLEOTIDES AND PLASMIDS 36
2.3 PLANT MATERIAL AND GROWTH CONDITIONS 36
2.4 BACTERIAL MATERIAL AND GROWTH CONDITIONS 37
2.5 MOLECULAR BIOLOGY TECHNIQUES 37
2.5.1 POLYMERASE CHAIN REACTION (PCR) 37
2.5.2 MOLECULAR CLONING 37
2.5.3 BACTERIAL TRANSFORMATION 38
2.5.4 PLANT TRANSFORMATION 38
2.5.5 DNA EXTRACTION 38
4
HTTP://D-NB.INFO/104409768X
2.5.6 PCR-BASED GENOTYPING 38
2.5.7 QUANTITATIVE REAL TIME RT-PCR 39
2.6 MICROARRAY ANALYSIS 39
2.7 LEAF AND CELL SIZE AND CELL NUMBER ANALYSIS 40
2.8 RELATIVE WATER CONTENT (RWC) MEASUREMENT 40
2.9 PROMOTER GUS ANALYSIS OF MYBL 40
2.10 ESTRADIOL INDUCTION EXPERIMENTS 40
2.11 CONFOCAL MICROSCOPY 41
2.12 SEED GERMINATION ASSAY AND STRESS TREATMENT 41
2.13 CHROMATIN IMMUNOPRECIPITATION-QUANTITATIVE PCR 42
2.14 IN-VITRO EXPRESSION AND ELECTROPHORETIC-MOBILITY SHIFT ASSAY (EMSA)
42
2.15 ABA EXTRACTION AND MEASUREMENT 43
2.16 SECONDARY METABOLITE PROFILING BY LC-MS 44
3 RESULTS 45
3.1 A MYBL TRANSCRIPTION FACTOR AFFECTS SALT TOLERANCE IN ARABIDOPSIS 45
3.2 THE MYBL MUTANT DISPLAYS ALTERED LEAF SHAPE 46
3.3 EXPRESSION AND LOCALIZATION OF THE MYBL 47
3.4 EXPRESSION OF MYBL IS AFFECTED BY ABIOTIC STRESS AND 49
CIRCADIAN RHYTHM 49
3.5 THE MYBL AFFECTS ABA RELATED PROCESSES 50
3.6 OVER-EXPRESSION OF MYBL REVERSES THE MYBL PHENOTYPES 51
3.7 OVER-EXPRESSION OF MYBL REVERSES THE EXPRESSION CHANGES 53
OBSERVED IN MYBL 53
3.8 THE MYBL AND MYBL OVER-EXPRESSING LINES SHOW ABA-RELATED PHENOTYPES
54
3.9 THE MYBL IS INDUCED BY ABIOTIC STRESS, BUT NOT BY ABA 56
3.10 THE MYBL DIRECTLY ACTIVATES SEVERAL GENES IN THE ABA 58
BIOSYNTHESIS AND SIGNALING PATHWAYS 58
3.11 INDUCTION OF MYBL INDUCES THE EXPRESSION OF ITS TARGET 61
GENES 61
3.12 STRESS INDUCTION OF THE MYBL TARGET GENES IS CHANGED IN AN 62
UNEXPECTED WAY IN THE MYBL MUTANT 62
3.13 MUTATIONS IN MYBL CONFERS PLANTS WITH DROUGHT TOLERANCE AND
INABILITY TO ACCUMULATE
ABA 64
3.14 THE MYBL PROTEIN AFFECTS SECONDARY METABOLITES 65
4 DISCUSSION 67
4.1 MYBL REGULATES ATNCED3, AND THEREBY CONTROLS ENDOGENOUS ABA LEVEL 67
4.2 MYBL REGULATES STRESS RESPONSE VIA TARGETING OF RD26 AND ANAC019 69
5
4.3 MYBL AND RD26/ANAC019 SHARE COMMON DOWNSTREAM 71
TARGETS 71
4.4 MYBL MIGHT BE INVOLVED IN REDOX STATE AND CIRCADIAN CLOCK REGULATION
72
4.5 MYBL REGULATES GENES INVOLVED IN SECONDARY METABOLISM 74
4.6 MUTATIONS IN MYBL CAUSE GROWTH DEFECTS 74
4.7 POTENTIAL CELLULAR REGULATORS OF MYBL 75
4.8 PROPOSED MODEL FOR MYBL FUNCTION 77
5 APPENDIX 79
6 REFERENCES 85
CURRICULUM VITAE 101
6
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| any_adam_object | 1 |
| author | Wang, Ting 1982- |
| author_GND | (DE-588)1043304789 |
| author_facet | Wang, Ting 1982- |
| author_role | aut |
| author_sort | Wang, Ting 1982- |
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| bvnumber | BV041376785 |
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| dewey-full | 571.742364 |
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| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.742364 |
| dewey-search | 571.742364 |
| dewey-sort | 3571.742364 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-12-20T16:46:04Z |
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| language | English |
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| physical | 102 S. Ill., graph. Darst. |
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| spellingShingle | Wang, Ting 1982- A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis Schmalwand Arabidopsis (DE-588)4179774-7 gnd Blattentwicklung (DE-588)4145843-6 gnd Microarray (DE-588)4544227-7 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Stressreaktion (DE-588)4138565-2 gnd |
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| title | A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis |
| title_auth | A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis |
| title_exact_search | A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis |
| title_full | A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis von Ting Wang |
| title_fullStr | A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis von Ting Wang |
| title_full_unstemmed | A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis von Ting Wang |
| title_short | A novel R2R3 MYB-like transcription factor regulates ABA mediated stress response and leaf growth in Arabidopsis |
| title_sort | a novel r2r3 myb like transcription factor regulates aba mediated stress response and leaf growth in arabidopsis |
| topic | Schmalwand Arabidopsis (DE-588)4179774-7 gnd Blattentwicklung (DE-588)4145843-6 gnd Microarray (DE-588)4544227-7 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Stressreaktion (DE-588)4138565-2 gnd |
| topic_facet | Schmalwand Arabidopsis Blattentwicklung Microarray Transkriptionsfaktor Stressreaktion Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026824832&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wangting anovelr2r3mybliketranscriptionfactorregulatesabamediatedstressresponseandleafgrowthinarabidopsis |