Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
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| Schlagwörter: | |
| Links: | http://mediatum.ub.tum.de/node?id=1135413 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20130131-1135413-0-4 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025925192&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 150 Blatt Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Nitsche, Ulrich Peter |e Verfasser |0 (DE-588)1037421043 |4 aut | |
| 245 | 1 | 0 | |a Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer |c Ulrich Peter Nitsche |
| 264 | 1 | |c 2012 | |
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| 912 | |a ebook | ||
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Datensatz im Suchindex
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| adam_text | IMAGE 1
INDEX
ABBREVIATIONS 6
1 INTRODUCTION - COLORECTAL CANCER 9
1.1 GLOBAL BURDEN 9
1.2 CLINICAL IMPLICATIONS 9
1.3 CLINICAL AND MOLECULAR GENETIC RISK ESTIMATION 12
1.4 PATHWAYS OF COLORECTAL CARCINOGENESIS 13
1.4.1 CHROMOSOMAL VERSUS MICROSATELLITE INSTABILITY 14
1.4.2 FROM THE VOGELGRAM TO THE ERA OF WHOLE GENOME SEQUENCING 15
1.4.3 FROM THE LINEAR ADENOMA-CARCINOMA SEQUENCE TO THREE MAJOR PATHWAYS
OF COLORECTAL CANCER 17
1.5 BIOMARKERS FOR COLORECTAL CANCER 18
1.5.1 KRASANDBRAF 19
1.5.2 MICROSATELLITE INSTABILITY 21
1.5.3 WNT PATHWAY 24
1.5.4 SASH1 26
1.5.5 MACC1 28
1.5.6 FURTHER CURRENTLY DISCUSSED BIOMARKERS FOR RISK PREDICTION IN
COLORECTAL
CANCER 30
2 AIM OF THE WORK 35
3 MATERIAL 36
3.1 CHEMICALS 36
3.2 SINGLE-USE DEVICES 38
3.3 KITS / COMPOSITIONS 39
3.4 MULTI-USE / TECHNICAL DEVICES 40
3.5 SOFTWARE 41
3.6 CELLS 42
3.7 ANTIBODIES, REAGENTS 42
3.8 PRIMER, SEQUENCES 43
4 METHODS 44
4.1 PROTOCOLS 44
4.2 PATIENT TISSUE AND DATA 61
3
HTTP://D-NB.INFO/1037421175
IMAGE 2
4.3 STATISTICAL ANALYSIS 62
5 RESULTS 64
5.1 PART I: MOLECULAR GENETIC CHARACTERIZATION OF COLON CANCER SAMPLES..
64
5.1.1 FEASIBILITY OF BIOMARKERS FOR RISK PREDICTION IN DISEASE STAGE II
64
5.1.2 MOLECULAR CHANGES DURING THE DEVELOPMENT OF METASTASIS 80
5.2 PART II: IN VITRO CHARACTERIZATION OF THE CANDIDATE
METASTASIS-MARKERS
SASH1 AND MACC1 82
5.2.1 EXPRESSION OF SASH1 AND MACC1 DIFFERS IN CELL LINES AND TISSUE 82
5.2.2 MACC1 EXPRESSION IS REGULATED BY HGF STIMULATION 87
5.2.3 ANALYSIS OF PUTATIVE INTERACTIONS BETWEEN MACC1 AND SASH1
EXPRESSION, AND THEIR ROLE IN CANCER CELL BIOLOGY 89
6 DISCUSSION 106
6.1 COLORECTAL CANCER 106
6.2 AIM OF THIS THESIS 106
6.3 A NEW INTEGRATIVE PANEL OF BIOMARKERS FOR METASTASIS-RISK PREDICTION
IN
COLORECTAL CANCER 107
6.4 RISK PREDICTION FOR STAGE II COLON CANCER PATIENTS 109
6.5 SASH1 AND MACC1 IN STAGE II PATIENTS 110
6.6 ESTABLISHED PATHWAYS OF COLORECTAL CANCER 111
6.7 MOLECULAR CHARACTERISTICS OF LIVER METASTASIS IN COMPARISON TO THE
PRIMARY COLORECTAL LESIONS 116
6.8 IS THERE INTERACTION BETWEEN SASH1 AND MACC1, TWO NEW
METASTASISASSOCIATED GENES, ON MRNA OR PROTEIN LEVEL? 117
6.9 EXPRESSION PATTERN OF MACC1 AND SASH1 IN CELL LINES 118
6.10 THE ROLE OF MACC1 IN THE CONTEXT OF ALTERED SIGNALING PATHWAYS IN
COLORECTAL CANCER 120
6.11 KNOCKDOWN OF SASH1 AND MACC1 REVEALS CELL-TYPE SPECIFIC EVIDENCE
FOR MUTUAL REGULATION 121
6.12 SASH1/MACC1: NO EVIDENCE FOR MUTUAL PROTEIN-PROTEIN INTERACTIONS
122
6.13 STABILITY AND HALF-LIFE OF MACC1 TRANSCRIPT IS NOT INFLUENCED BY
SASH1
EXPRESSION 123
6.14 PROTEIN STABILITY OF MACC1 AND SASH1 125
6.15 MACC1 ACTIVATES CELL MIGRATION, BUT NOT CELL PROLIFERATION 126
6.16 OUTLOOK 127
4
IMAGE 3
7 SYNOPSIS 129
7.1 ENGLISH 129
7.2 DEUTSCH 130
8 REFERENCES 131
9 LIST OF TABLES AND FIGURES 146
9.1 TABLES 146
9.2 FIGURES 146
10 PUBLICATIONS 148
5
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| indexdate | 2024-12-20T16:27:48Z |
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| spellingShingle | Nitsche, Ulrich Peter Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer |
| subject_GND | (DE-588)4113937-9 |
| title | Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer |
| title_auth | Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer |
| title_exact_search | Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer |
| title_full | Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer Ulrich Peter Nitsche |
| title_fullStr | Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer Ulrich Peter Nitsche |
| title_full_unstemmed | Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer Ulrich Peter Nitsche |
| title_short | Molecular characterization of metastasis-associated signaling pathways and their interactions in colon cancer |
| title_sort | molecular characterization of metastasis associated signaling pathways and their interactions in colon cancer |
| topic_facet | Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1135413 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20130131-1135413-0-4 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025925192&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT nitscheulrichpeter molecularcharacterizationofmetastasisassociatedsignalingpathwaysandtheirinteractionsincoloncancer |
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