Function of the transcription factor Fra1 in adipogenesis: = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
|
| Schlagwörter: | |
| Links: | https://open.fau.de/handle/openfau/2798 http://d-nb.info/1029374260/34 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-40161 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025745519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 106 S. graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV040767062 | ||
| 003 | DE-604 | ||
| 005 | 20150206 | ||
| 007 | t| | ||
| 008 | 130221s2012 gw d||| m||| 00||| eng d | ||
| 015 | |a 13,O02 |2 dnb | ||
| 016 | 7 | |a 1029374260 |2 DE-101 | |
| 035 | |a (OCoLC)828809888 | ||
| 035 | |a (DE-599)DNB1029374260 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE-BY | ||
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 |a DE-29T | ||
| 082 | 0 | |a 610 | |
| 084 | |a 610 |2 sdnb | ||
| 100 | 0 | |a Luther Julia |e Verfasser |0 (DE-588)1031527095 |4 aut | |
| 245 | 1 | 0 | |a Function of the transcription factor Fra1 in adipogenesis |b = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese |c von Julia Iris Luther |
| 246 | 1 | 1 | |a Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese |
| 264 | 1 | |c 2012 | |
| 300 | |a 106 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a Fettgewebe |0 (DE-588)4154224-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Transkriptionsfaktor |0 (DE-588)4303350-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fettsucht |0 (DE-588)4016953-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Fettgewebe |0 (DE-588)4154224-1 |D s |
| 689 | 0 | 1 | |a Transkriptionsfaktor |0 (DE-588)4303350-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Fettsucht |0 (DE-588)4016953-4 |D s |
| 689 | 1 | 1 | |a Transkriptionsfaktor |0 (DE-588)4303350-7 |D s |
| 689 | 1 | |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:29-opus-40161 |
| 856 | 4 | |u https://open.fau.de/handle/openfau/2798 |x Verlag |z kostenfrei |3 Volltext | |
| 856 | 4 | |u http://d-nb.info/1029374260/34 |x Langzeitarchivierung Nationalbibliothek | |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-40161 |x Resolving-System | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025745519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-025745519 | |
Datensatz im Suchindex
| DE-BY-OTHR_katkey | 5087725 |
|---|---|
| DE-BY-TUM_katkey | 1912393 |
| _version_ | 1831256852344602625 |
| adam_text | IMAGE 1
TABLE O F CONTENTS
TABLE OF CONTENTS
ABSTRACT 7
ZUSAMMENFASSUNG 8
1 INTRODUCTION 10
1.1 BONE 10
1.1.1 BONE TISSUE: COMPOSITION AND FUNCTION 10
1.1.2 BONE DEVELOPMENT 10
1.1.3 BONE CELLS AND BONE REMODELING 11
1.2 ADIPOSE TISSUE 12
1.2.1 BROWN AND WHITE ADIPOSE TISSUE 12
1.2.2 FACTORS PRODUCED BY ADIPOSE TISSUE: ADIPOKINES 13
1.2.3 THE CO-REGULATION O F ADIPOSE TISSUE AND BONE 14
1.3 MESENCHYMAL CELL DIFFERENTIATION 15
1.3.1 DIFFERENTIATION O F MESENCHYMAL STROMAL CELLS (MSC) IS
1.3.2 CHONDROCYTE DIFFERENTIATION 16
1.3.3 MYOBLAST AND BROWN ADIPOCYTE DIFFERENTIATION 17
1.3.4 TRANSCRIPTIONAL CONTROL O F ADIPOGENESIS 17
1.3.5 OSTEOBLAST DIFFERENTIATION 19
1.3.6 RECIPROCAL REGULATION O F OSTEOBLASTOGENESIS AND ADIPOGENESIS 20
1.4 AP-1 TRANSCRIPTION FACTOR 20
1.4.1 STRUCTURE AND COMPOSITION O F THE AP-1 TRANSCRIPTION FACTOR 21
1.4.2 REGULATION OF TRANSCRIPTIONAL ACTIVITY 21
1.4.3 FUNCTIONS O F AP-1 22
1.4.4 FUNCTIONS O F AP-1 IN BONE DEVELOPMENT 23
1.4.5 FUNCTIONS O F AP-1 IN ADIPOCYTE DEVELOPMENT 24
1.5 AIM OF THE WORK 24
2. MATERIALS AND METHODS 26
2.1 MATERIALS 26
2.1.1 BUFFER AND STAINING SOLUTIONS 26
2.1.2 CELL CULTURE MEDIA AND SUPPLEMENTS 29
2.1.3 OLIGONUCLEOTIDES 30
- 4 -
HTTP://D-NB.INFO/1031268510
IMAGE 2
TABLE O F CONTENTS
2.1.4 ANTIBODIES 32
2.1.5 PLASMIDS 33
2.1.6 MOLECULAR WEIGHT MARKER AND LOADING DYE 33
2.1.7 REAGENTS FOR CELL CULTURE 33
2.1.8 KITS AND ENZYMES 34
2.1.9 CHEMICALS 36
2.1.10 CONSUMABLES 38
2.1.11 LABORATORY INSTRUMENTS 38
2.2 METHODS 40
2.2.1 ANIMAL EXPERIMENTS 40
2.2.2 CELL CULTURE 41
2.2.3 METHODS TO STUDY RNA 4 3
2.2.4 METHODS TO STUDY DNA 44
2.2.5 METHODS TO STUDY PROTEIN 45
2.2.6 SEQUENCE ANALYSIS 48
2.2.7 STATISTICAL ANALYSIS 48
3 RESULTS 50
3.1 NO CLEAR ADIPOGENIC PHENOTYPE O F AP2 -ERE F R A L ^ M I C E 50
3.2 LIPODYSTROPHY PHENOTYPE IN MICE OVEREXPRESSING FRAL 52
3.2.1 SEVERE LIPODYSTROPHY IN FRAL TRANSGENIC MICE 52
3.2.2 NORMAL BROWN ADIPOSE TISSUE (BAT) 54
3.2.3 IMMATURE WHITE ADIPOSE TISSUE (WAT) IN MICE OVEREXPRESSING FRAL 55
3.2.4 NORMAL FOOD INTAKE AND INSULIN LEVELS IN FRAL TRANSGENIC MICE 57
3.2.5 INCREASED INSULIN SENSITIVITY, SERUM TRIGLYCERIDE AND
NON-ESTERIFIED FATTY ACID LEVELS
IN FRAL TRANSGENIC MICE 58
3.2.6 NO ECTOPIC LIPID DEPOSITION IN LIVER OR MUSCLE O F FRAL TRANSGENIC
MICE 60
3.2.7 DECREASED EXPRESSION OF GENES INVOLVED IN LIPOGENESIS AND
LIPOLYSIS IN THE ADIPOSE
TISSUE O F FRAL TG MICE 60
3.2.8 NO CORRECTION O F THE LIPODYSTROPHIC PHENOTYPE BY HIGH FAT DIET 63
3.3 REDUCED DIFFERENTIATION TO ADIPOCYTES IN THE WAT OF FRAL TRANSGENIC
MICE 65
3.3.1 NO TRANSDIFFERENTIATION O F WAT TOWARD BAT IN FRAL TRANSGENIC MICE
65
3.3.2 EXPRESSION O F ADIPOKINES IN WHITE ADIPOSE TISSUE O F FRAL
TRANSGENIC MICE 65
- 5 -
IMAGE 3
TABLE O F CONTENTS
3.3.3 NO CHANGE IN THE EXPRESSION O F MARKERS FOR ADIPOCYTE PROGENITOR
CELLS IN WHITE
ADIPOSE TISSUE O F FRAL TRANSGENIC MICE 67
3.3.4 NO CHANGE IN AP-1 EXPRESSION IN THE WAT O F FRAL TRANSGENIC MICE
67
3.3.5 DECREASED C/EBPA EXPRESSION IN WHITE ADIPOSE TISSUE OVEREXPRESSING
FRAL 68
3.4 IN VITRO ANALYSIS OF THE ROLE OF FRAL DURING ADIPOCYTE
DIFFERENTIATION 71
3.4.1 CELL AUTONOMOUS DECREASED ADIPOGENESIS O F FRAL TRANSGENIC
MESENCHYMAL CELLS 71
3.4.2 NORMAL RESPONSES O F FRAL TRANSGENIC MESENCHYMAL PROGENITORS TO
ADIPOGENIC
STIMULATION 72
3.4.3 FRAL OVEREXPRESSION DIRECTLY BLOCKS ADIPOGENESIS 74
3.4.4 NO DETECTABLE REGULATION O F C/EBPA EXPRESSION VIA BINDING O F
FRAL TO C/EBPP 75
3.4.5 FRAL DIRECTLY INHIBITS THE TRANSCRIPTION O F C/EBPA 76
4. DISCUSSION 79
4.1 PHENOTYPE OF THE FRAL TRANSGENIC AND THE FRAL KNOCKOUT MOUSE 79
4.2 FRAL OVEREXPRESSION DECREASES ADIPOSE TISSUE MASS 80
4.3 FRAL REGULATES ADIPOCYTE DIFFERENTIATION IN A CELL AUTONOMOUS MANNER
81
4.4 FRAL DIRECTLY REGULATES ADIPOCYTE DIFFERENTIATION BY REDUCING C/EBPA
EXPRESSION 82
4.5 INFLUENCE OF THE REDUCED AMOUNT OF MATURE ADIPOCYTES ON METABOLISM
84
4.6 PHENOTYPE OF MICE WITH AN ADIPOCYTE-SPECIFIC DEFICIENCY OF FRAL 86
4.7 CONCLUSIONS 86
5 REFERENCES 88
6 APPENDIX 100
6.1 ABBREVIATIONS 100
6.2 CLONED SEQUENCE OF THE CEJPAPROMOTER 102
6.3 ACKNOWLEDGEMENTS 104
6.4 PUBLICATIONS AND PRESENTATIONS 105
- 6 -
|
| any_adam_object | 1 |
| author | Luther Julia |
| author_GND | (DE-588)1031527095 |
| author_facet | Luther Julia |
| author_role | aut |
| author_sort | Luther Julia |
| author_variant | l j lj |
| building | Verbundindex |
| bvnumber | BV040767062 |
| collection | ebook |
| ctrlnum | (OCoLC)828809888 (DE-599)DNB1029374260 |
| dewey-full | 610 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610 |
| dewey-search | 610 |
| dewey-sort | 3610 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02289nam a2200517zc 4500</leader><controlfield tag="001">BV040767062</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20150206 </controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">130221s2012 gw d||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">13,O02</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1029374260</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)828809888</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1029374260</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-BY</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield><subfield code="a">DE-29T</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">610</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Luther Julia</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1031527095</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Function of the transcription factor Fra1 in adipogenesis</subfield><subfield code="b">= Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese</subfield><subfield code="c">von Julia Iris Luther</subfield></datafield><datafield tag="246" ind1="1" ind2="1"><subfield code="a">Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">106 S.</subfield><subfield code="b">graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Erlangen-Nürnberg, Univ., Diss., 2012</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Fettgewebe</subfield><subfield code="0">(DE-588)4154224-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Transkriptionsfaktor</subfield><subfield code="0">(DE-588)4303350-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Fettsucht</subfield><subfield code="0">(DE-588)4016953-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Fettgewebe</subfield><subfield code="0">(DE-588)4154224-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Transkriptionsfaktor</subfield><subfield code="0">(DE-588)4303350-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Fettsucht</subfield><subfield code="0">(DE-588)4016953-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Transkriptionsfaktor</subfield><subfield code="0">(DE-588)4303350-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:29-opus-40161</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://open.fau.de/handle/openfau/2798</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">http://d-nb.info/1029374260/34</subfield><subfield code="x">Langzeitarchivierung Nationalbibliothek</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-40161</subfield><subfield code="x">Resolving-System</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025745519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025745519</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040767062 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T16:25:16Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025745519 |
| oclc_num | 828809888 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T |
| physical | 106 S. graph. Darst. |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spellingShingle | Luther Julia Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese Fettgewebe (DE-588)4154224-1 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Fettsucht (DE-588)4016953-4 gnd |
| subject_GND | (DE-588)4154224-1 (DE-588)4303350-7 (DE-588)4016953-4 (DE-588)4113937-9 |
| title | Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese |
| title_alt | Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese |
| title_auth | Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese |
| title_exact_search | Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese |
| title_full | Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese von Julia Iris Luther |
| title_fullStr | Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese von Julia Iris Luther |
| title_full_unstemmed | Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese von Julia Iris Luther |
| title_short | Function of the transcription factor Fra1 in adipogenesis |
| title_sort | function of the transcription factor fra1 in adipogenesis die funktion des transkriptionsfaktors fra1 in der adipogenese |
| title_sub | = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese |
| topic | Fettgewebe (DE-588)4154224-1 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Fettsucht (DE-588)4016953-4 gnd |
| topic_facet | Fettgewebe Transkriptionsfaktor Fettsucht Hochschulschrift |
| url | https://open.fau.de/handle/openfau/2798 http://d-nb.info/1029374260/34 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-40161 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025745519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT lutherjulia functionofthetranscriptionfactorfra1inadipogenesisdiefunktiondestranskriptionsfaktorsfra1inderadipogenese AT lutherjulia diefunktiondestranskriptionsfaktorsfra1inderadipogenese |