Ion channels: methods and protocols
Gespeichert in:
| Weitere beteiligte Personen: | |
|---|---|
| Format: | Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2013
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
998 |
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025670290&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | XIV, 440 S. Ill., graph. Darst. |
| ISBN: | 9781627033503 9781627033510 |
Internformat
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| 245 | 1 | 0 | |a Ion channels |b methods and protocols |c ed. by Nikita Gamper |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2013 | |
| 300 | |a XIV, 440 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1819316118330802176 |
|---|---|
| adam_text | Titel: Ion channels
Autor: Gamper, Nikita
Jahr: 2013
Contents
Preface.............................................................. v
Contributors.......................................................... xi
Part I Cloning and Heterologous Expression of Ion Channel Genes
1 Approaches to Cloning of Pain-Related Ion Channel Genes.................. 3
Armen N. Akopian
2 Mammalian Expression Systems and Transaction Techniques................. 21
Daunia Laurenti and Lezanne Ooi
3 Use of Escherichia coli for the Production and Purification
of Membrane Proteins.............................................. 33
Vincent G.L. Postis, Andrea E. Rawlings, Amelia Lesiuk,
and Stephen A. Baldwin
4 Transient Overexpression of Genes in Neurons Using Nucleofection............ 55
Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper
5 Viral Gene Delivery: Optimized Protocol for Production
of High Titer Lentiviral Vectors....................................... 65
James Hewinson, Julian F.R. Paton, and Sergey Kasparov
Part II Electrophysiological Methods to Study Ion
Channel Functions
6 Two-Electrode Voltage Clamp........................................ 79
Bingcai Guan, Xingjuan Chen, and Hailin Zhang
7 Conventional Micropipette-Based Patch Clamp Techniques.................. 91
Jonathan D. Lippiat and David C. Wrighton
8 Recording of Ion Channel Activity in Planar Lipid Bilayer Experiments.......... 109
Eleonora Zakharian
9 Recording Macroscopic Currents in Large Patches
from Xenopus Oocytes.............................................. 119
Tibor Rohacs
10 Combined Single-Channel and Macroscopic Recording
Techniques to Analyze Gating Mechanisms of the Large Conductance
Ca2+ and Voltage Activated (BK) Potassium Channel........................ 133
Nguyen V. Nguyen, Aleksandra Gruslova,
Wojciech A. Kosiba, and Bin Wang
11 Perforated Whole-Cell Patch-Clamp Recording........................... 149
John E. Linley
12 Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons........... 159
Jizhe Hao, Jerome Ruel, Bertrand Coste, Tann Roudaut,
Marcel Crest, and Patrick Delmas
vii
vili
Contents
13 Automated Planar Patch-Clamp....................................... 171
Carol J. Millikan and Clemens Möller
14 Recording Single-Channel Currents Using
Smart Patch-Clamp Technique...................................... 189
Anamika Bhargava and Julia Gorelik
Part III Imaging and Fluorescence Methods to Study Ion Channels
15 Using Total Internal Reflection Fluorescence Microscopy
to Observe Ion Channel Trafficking and Assembly......................... 201
Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy,
and Andrew Tinker
16 Förster Resonance Energy Transfer-Based Imaging
at the Cell Surface of Live Cells....................................... 209
Sonya M. Bierbower and Mark S. Shapiro
17 The Use of Dansyl-Calmodulin to Study Interactions
with Channels and Other Proteins..................................... 217
Alessandro Alaimo, Covadonga Malo, Pilar Areso,
Kerman Aloria, Oscar Millet, and Alvaro Villarroel
18 Imaging and Quantification of Recycled KATp Channels...................... 233
Christofher J. Cocker oft
Part IV Biochemical and Structure-Functional Approaches
in Ion Channel Studies
19 Generation of Antibodies That Are Externally Acting
Isoform-Specific Inhibitors of Ion Channels.............................. 245
Jacqueline Naylor and David J. Beech
20 Site-Directed Mutagenesis to Study the Structure-Function
Relationships of Ion Channels......................................... 257
Wei Tang and Lin-Hua Jiang
21 Cysteine-Based Cross-Linking Approach to Study
Inter-domain Interactions in Ion Channels............................... 267
Lin-Hua Jiang
22 Analysis of Ca2+ Binding Sites in the MthK RCK Domain
by X Ray Crystallography............................................ 277
Frank J. Smith and Brad S. Rothberg
23 Isotope Labeling Strategies for Analysis of an Ion
Channel Cytoplasmic Domain by NMR Spectroscopy....................... 289
Karin Abarca-Heidemann, Elke Duchardt-Ferner,
Jens Woehnert, and Brad S. Rothberg
Part V Studying Ion Channels in Native Tissues
24 Recording Dendritic Ion Channel Properties and Function
from Cortical Neurons.............................................. 303
Mala M. Shah
Contents ix
25 M-Current Recording from Acute DRG Slices............................ 311
Kirstin E. Rose, Sylvain Gigout, and Nikita Gamper
26 Studying Ion Channels in Human Erythrocytes
by Direct and Indirect Means......................................... 321
Stephan M. Huber
27 Recording Ion Channels in Isolated, Split-Opened Tubules................... 341
Elena Mironova, Vladislav Bug ay, Oleh Pochynyuk,
Alexander Staruschenko, and James D. Stockand
28 Single-Channel Analysis of TRPC Channels
in the Podocytes of Freshly Isolated Glomeruli............................ 355
Daria V. Ilatovskaya and Alexander Staruschenko
29 Ca2+ Imaging as a Tool to Assess TRP Channel Function
in Murine Distal Nephrons........................................... 371
Mykola Mamenko, Ole# Zaika, Roger G. O Neil, and Oleh Pochynyuk
30 Patch-Clamping Drosophila Sensory Neurons............................. 385
Volodymyr Kucher, Benjamin A. Eaton,
James D. Stockand, and Nina Boiko
Part VI Ion Channels as Research Tools
31 Production and Validation of Recombinant Adeno-Associated Virus
for Channelrhodopsin Expression in Neurons............................. 401
John Y. Lin
32 Optical Control of Ligand-Gated Ion Channels........................... 417
Stephanie Szobota, Catherine McKenzie, and Harald Janovjak
Index................................................................... 437
|
| any_adam_object | 1 |
| author2 | Gamper, Nikita |
| author2_role | edt |
| author2_variant | n g ng |
| author_facet | Gamper, Nikita |
| building | Verbundindex |
| bvnumber | BV040689511 |
| classification_rvk | WC 4150 WC 4460 WE 5460 |
| ctrlnum | (OCoLC)843488750 (DE-599)BVBBV040689511 |
| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV040689511 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T16:23:25Z |
| institution | BVB |
| isbn | 9781627033503 9781627033510 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025670290 |
| oclc_num | 843488750 |
| open_access_boolean | |
| owner | DE-11 DE-355 DE-BY-UBR DE-12 DE-20 |
| owner_facet | DE-11 DE-355 DE-BY-UBR DE-12 DE-20 |
| physical | XIV, 440 S. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spellingShingle | Ion channels methods and protocols Methods in molecular biology Ion Channels Molekularbiologische Methode (DE-588)7650063-9 gnd Ionenkanal (DE-588)4138699-1 gnd |
| subject_GND | (DE-588)7650063-9 (DE-588)4138699-1 |
| title | Ion channels methods and protocols |
| title_auth | Ion channels methods and protocols |
| title_exact_search | Ion channels methods and protocols |
| title_full | Ion channels methods and protocols ed. by Nikita Gamper |
| title_fullStr | Ion channels methods and protocols ed. by Nikita Gamper |
| title_full_unstemmed | Ion channels methods and protocols ed. by Nikita Gamper |
| title_short | Ion channels |
| title_sort | ion channels methods and protocols |
| title_sub | methods and protocols |
| topic | Ion Channels Molekularbiologische Methode (DE-588)7650063-9 gnd Ionenkanal (DE-588)4138699-1 gnd |
| topic_facet | Ion Channels Molekularbiologische Methode Ionenkanal |
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