Structural studies on the association of filamentous proteins in the human M-Bands:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2011
|
| Schlagwörter: | |
| Links: | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72410 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025287838&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | Zsfassung in dt. und engl. Sprache |
| Umfang: | 137, XVI S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV040439988 | ||
| 003 | DE-604 | ||
| 005 | 20120928 | ||
| 007 | t| | ||
| 008 | 120925s2011 xx ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)815930424 | ||
| 035 | |a (DE-599)BVBBV040439988 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 | ||
| 100 | 1 | |a Sauer, Florian |d 1982- |e Verfasser |0 (DE-588)1026248485 |4 aut | |
| 245 | 1 | 0 | |a Structural studies on the association of filamentous proteins in the human M-Bands |c von Florian Sauer |
| 246 | 1 | 3 | |a Strukturelle Studien zur Zusammenlagerung filamentöser Protein in humanen M-Banden |
| 264 | 1 | |c 2011 | |
| 300 | |a 137, XVI S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. und engl. Sprache | ||
| 502 | |a Würzburg, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a Muskelkontraktion |0 (DE-588)4170858-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Quer gestreifte Muskulatur |0 (DE-588)4176716-0 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Muskelkontraktion |0 (DE-588)4170858-1 |D s |
| 689 | 0 | 1 | |a Quer gestreifte Muskulatur |0 (DE-588)4176716-0 |D s |
| 689 | 0 | |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:20-opus-72410 |
| 856 | 4 | 1 | |u https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72410 |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025287838&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-025287838 | |
Datensatz im Suchindex
| DE-BY-TUM_katkey | 1875076 |
|---|---|
| _version_ | 1821935048729821184 |
| adam_text | IMAGE 1
TABLE O F CONTENTS
TABLE OF CONTENTS
ABSTRACT I
ZUSAMMERIFASSUNG ILL
TABLE OF CONTENTS V
LIST OF FIGURES VII
LIST OF TABLES VIII
ABBREVIATIONS IX
1 INTRODUCTION 1
1.1 VERTEBRATE STRIATED MUSCLE 1
1.1.1 GENERAL ARCHITECTURE 1
1.1.2 THE SARCOMERE 2
1.1.3 SARCOMERE CONTRACTION: T H E SLIDING F I L A M E N T M O D E L 5
1.2 THE M-BAND: A CROSSLINK FOR THE THICK FILAMENTS 5
1.2.1 COMPONENTS O F T H E SARCOMERIC CYTOSKELETON ASSOCIATE IN T H E M
- B A N D 8
1.2.1.1 TITIN: THE THIRD FILAMENT 8
1.2.1.1.1 TITIN CONNECTS Z-DISKS TO THE M-BANDS 9
1.2.1.1.2 THE CENTRAL PORTION O F TITIN ACTS AS SARCOMERIC RULER AND
SPRING 13
1.2.1.2 MEMBERS O F THE MYOMESIN FAMILY: THICK FILAMENT CROSS-LINKERS 15
1.2.1.3 OBSCURIN AND OBSL1 18
1.2.1.3.1 OBSCURIN AND OBSL1: NOVEL SARCOMERIC FILAMENTS 18
1.2.1.3.2 OBSCURIN AND OBSL1 ASSEMBLE WITH TITIN AND MYOMESIN-1 IN THE
M-BANDS 19
1.2.2 ALTERATIONS O F M - B A N D PROTEINS ARE INVOLVED IN T H E D E V E
L O P M E N T O F HEREDITARY
DISEASES 2 1
1.2.3 A C U R R E N T MODEL O F T H E VERTEBRATE M - B A N D 22
1.3 AIM OF THE WORK 24
2 MATERIALS AND METHODS 25
2.1 MATERIALS 25
2.1.1 CHEMICALS, KITS AND CONSUMABLES 25
2.1.2 CULTURE MEDIA 2 6
2.1.3 ENZYMES, PRIMERS, VECTORS AND EXPRESSION STRAINS 2 6
2.1.4 BUFFERS F O R PROTEIN PURIFICATION AND CHARACTERIZATION 27
2.1.5 MACHINES AND COLUMNS 2 8
2.1.6 SOFTWARE AND DATABASES 2 9
2.2 METHODS 30
2.2.1 CLONING O F EXPRESSION CONSTRUCTS AND SITE DIRECTED MUTAGENESIS 3
0
2.2.2 PROTEIN EXPRESSION IN E. COLI 32
2.2.3 PROTEIN PURIFICATION 33
2.2.3.1 IMAC AFFINITY PURIFICATION OF POLY-HISTIDINE TAGGED PROTEINS 33
2.2.3.2 PURIFICATION BY SIZE-EXCLUSION CHROMATOGRAPHY 34
2.2.4 PROTEIN AND PROTEIN COMPLEX CHARACTERIZATION 3 4
2.2.4.1 ESTIMATION O F PROTEIN CONCENTRATIONS 34
2.2.4.2 ESTIMATION OF PROTEIN WEIGHT AND PURITY BY SDS-PAGE 35
2.2.4.3 ESTIMATION OF SECONDARY STRUCTURE CONTENT BY CIRCULAR DICHROISM
35
2.2.4.4 COMPLEX CHARACTERIZATION BY ITC 36
2.2.4.5 X-RAY CRYSTALLOGRAPHY 37
2.2.4.5.1 PRINCIPLES 37
2.2.4.5.2 PHASE DETERMINATION 39
2.2.4.5.3 ESTIMATION O F DIFFRACTION DATA QUALITY 43
- V -
HTTP://D-NB.INFO/1026910498
IMAGE 2
TABLE O F CONTENTS
2.2.4.5.4 PROTEIN CRYSTALLIZATION 44
2.2.4.5.5 DATA COLLECTION, STRUCTURE SOLUTION AND REFINEMENT 46
2.2.4.5.6 STRUCTURE ANALYSIS AND VISUALIZATION 47
3 RESULTS AND DISCUSSION 4 8
3.1 CHARACTERIZATION OF THE HUMAN TITIN - OBSL1 / OBSCURIN HEAD - TO -
TAIL COMPLEX ... 49 3.1.1 RESULTS 4 9
3.1.1.1 TITIN M10 AND OBSCURIN/OBSLL I G L FORM A BINARY COMPLEX 49
3.1.1.2 CRYSTALLIZATION 50
3.1.1.3 DATA COLLECTION AND STRUCTURE SOLUTION 5 1
3.1.1.4 OVERALL STRUCTURE 53
3.1.1.5 COMPLEX FORMATION BY INTERMOLECULAR 15-SHEET FORMATION 54
3.1.1.6 CHARACTERIZATION O F THE INTERFACE AND DISEASE RELATED MUTATIONS
57
3.1.1.6.1 HOMOLOGY BETWEEN OBSL1 AND OBSCURIN 57
3.1.1.6.2 CALORIMETRIC CHARACTERIZATION 59
3.1.1.6.3 CHARACTERIZATION O F DISEASE RELATED MUTATIONS 59
3.1.2 DISCUSSION 65
3.1.2.1 A NOVEL VARIATION O F A KNOWN ASSEMBLY MODE 65
3.1.2.2 A SAFEGUARD FOR TITIN KINASE FUNCTION? 67
3.1.2.3 TWO HYPOTHETICAL MODELS FOR THE ORIGIN O F M10 LINKED MYOPATHIES
70
3.2 CHARACTERIZATION OF THE DIRECT ASSEMBLY BETWEEN TITIN AND MYOMESIN-1
74
3.2.1 RESULTS 7 4
3.2.1.1 PURIFICATION O F THE COMPLEX PARTNERS 74
3.2.1.2 IN VITRO TITIN DOES NOT SPONTANEOUSLY FORM A COMPLEX WITH
MYOMESIN-1 76
3.2.2 DISCUSSION 7 8
3.3 CHARACTERIZATION OF THE CENTRAL ASSEMBLY SITE OF MYOMESIN-1 80
3.3.1 RESULTS 8 0
3.3.1.1 MYOMESIN-1 DIMERIZATION IS MEDIATED BY DOMAIN 5 80
3.3.1.2 CRYSTALLIZATION OF THE MYOMESIN-1 MY5 DOMAIN 84
3.3.1.3 DATA COLLECTION, STRUCTURE SOLUTION AND REFINEMENT 86
3.3.1.4 CRYSTAL STRUCTURE O F THE MY5 DIMER 88
3.3.2 DISCUSSION 9 2
3.3.2.1 DIMERIZATION BY THREE DIMENSIONAL DOMAIN SWAPPING 92
3.3.2.2 BIOLOGICAL IMPLICATIONS: A HYPOTHETICAL MODEL FOR THE
INCORPORATION O F TETRAMERIC MYOMESIN-1 IN THE M-BANDS 95
3.4 CRYSTAL STRUCTURE OF TITIN M 4 100
3.4.1 RESULTS 100
3.4.1.1 CRYSTALLIZATION 100
3.4.1.2 DATA COLLECTION, STRUCTURE SOLUTION AND MODEL REFINEMENT 101
3.4.1.3 OVERALL STRUCTURE 106
3.4.1.4 DIMERIZATION O F M4 109
3.4.1.5 THE POSITION O F CYS22 IS UNIQUE WITHIN TITIN IG-LIKE DOMAINS
AND CONSERVED AMONG VERTEBRATES I L L
3.4.2 DISCUSSION 117
3.4.2.1 THE M4 ASSEMBLY MAY REPRESENT A TEMPORARY STABLE ANCHOR O F
TITIN IN THE M-BANDS 117
4 CONCLUSIONS AND FUTURE PERSPECTIVES 122
5 LITERATURE 125
APPENDIX XI
PUBLICATIONS XII
PRESENTATIONS AND PARTICIPATIONS XII
CONTRIBUTIONS XIII
ACKNOWLEDGEMENTS XIV
CURRICULUM VITAE XV
ERKLARUNG XVI
- V I -
|
| any_adam_object | 1 |
| author | Sauer, Florian 1982- |
| author_GND | (DE-588)1026248485 |
| author_facet | Sauer, Florian 1982- |
| author_role | aut |
| author_sort | Sauer, Florian 1982- |
| author_variant | f s fs |
| building | Verbundindex |
| bvnumber | BV040439988 |
| collection | ebook |
| ctrlnum | (OCoLC)815930424 (DE-599)BVBBV040439988 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01857nam a2200397 c 4500</leader><controlfield tag="001">BV040439988</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20120928 </controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">120925s2011 xx ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)815930424</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV040439988</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Sauer, Florian</subfield><subfield code="d">1982-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1026248485</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Structural studies on the association of filamentous proteins in the human M-Bands</subfield><subfield code="c">von Florian Sauer</subfield></datafield><datafield tag="246" ind1="1" ind2="3"><subfield code="a">Strukturelle Studien zur Zusammenlagerung filamentöser Protein in humanen M-Banden</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">137, XVI S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Zsfassung in dt. und engl. Sprache</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Würzburg, Univ., Diss., 2012</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Muskelkontraktion</subfield><subfield code="0">(DE-588)4170858-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Quer gestreifte Muskulatur</subfield><subfield code="0">(DE-588)4176716-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Muskelkontraktion</subfield><subfield code="0">(DE-588)4170858-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Quer gestreifte Muskulatur</subfield><subfield code="0">(DE-588)4176716-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:20-opus-72410</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72410</subfield><subfield code="x">Resolving-System</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025287838&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025287838</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040439988 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T16:15:01Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025287838 |
| oclc_num | 815930424 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| physical | 137, XVI S. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| record_format | marc |
| spellingShingle | Sauer, Florian 1982- Structural studies on the association of filamentous proteins in the human M-Bands Muskelkontraktion (DE-588)4170858-1 gnd Quer gestreifte Muskulatur (DE-588)4176716-0 gnd |
| subject_GND | (DE-588)4170858-1 (DE-588)4176716-0 (DE-588)4113937-9 |
| title | Structural studies on the association of filamentous proteins in the human M-Bands |
| title_alt | Strukturelle Studien zur Zusammenlagerung filamentöser Protein in humanen M-Banden |
| title_auth | Structural studies on the association of filamentous proteins in the human M-Bands |
| title_exact_search | Structural studies on the association of filamentous proteins in the human M-Bands |
| title_full | Structural studies on the association of filamentous proteins in the human M-Bands von Florian Sauer |
| title_fullStr | Structural studies on the association of filamentous proteins in the human M-Bands von Florian Sauer |
| title_full_unstemmed | Structural studies on the association of filamentous proteins in the human M-Bands von Florian Sauer |
| title_short | Structural studies on the association of filamentous proteins in the human M-Bands |
| title_sort | structural studies on the association of filamentous proteins in the human m bands |
| topic | Muskelkontraktion (DE-588)4170858-1 gnd Quer gestreifte Muskulatur (DE-588)4176716-0 gnd |
| topic_facet | Muskelkontraktion Quer gestreifte Muskulatur Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72410 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025287838&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT sauerflorian structuralstudiesontheassociationoffilamentousproteinsinthehumanmbands AT sauerflorian strukturellestudienzurzusammenlagerungfilamentoserproteininhumanenmbanden |