The expression of CPP fusion proteins in plastids:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
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| Schlagwörter: | |
| Links: | http://edoc.ub.uni-muenchen.de/14521/ http://d-nb.info/1024457389/34 https://nbn-resolving.org/urn:nbn:de:bvb:19-145219 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025237959&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 94 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a The expression of CPP fusion proteins in plastids |c Florian Mayer |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
CONTENTS
1 INTRODUCTION 9
1.1 THE GENETIC TRANSFORMATION O F PLANTS 9
1.2 PLASTIC! T R A N S F O R M A T I O N 9
1.3 CURRENT APPLIED ASPECTS O F PLASTID T R A N S F O R M A T I O N 1 1
1.4 CELL PENETRATING PEPTIDES (CPPS) 12
1.5 ARTEFACT DISCOVERY AND CPP MECHANISM 13
1.6 CLINICAL TRIALS AND F U T U R E CHALLENGES 15
1.7 RECENTLY: PLANTS AND CPPS 15
1.8 A I M O F THIS THESIS 18
2 RESULTS 19
2.1 EGFP V E C T O R SERIES: TRACKING CPP FUSION PROTEINS 19
2.2 PLASTID T R A N S F O R M A T I O N V E C T O R INTERMEDIATE
PUC18(C) 3 0
2.3 PAP1 V E C T O R SERIES: PROVIDING A PHYSIOLOGICAL CPP READ-OUT 3 1
2.4 PAH VECTORS SERIES: CPP FUSIONS F O R T H E CLINIC 3 6
3 DISCUSSION 4 1
3.1 EXPRESSION O F CPP FUSION PROTEINS IN TOBACCO PLASTIDS IS FEASIBLE 4
1
3.2 CPP FUSION PROTEINS ARE ENTRAPPED IN T H E ORGANELLE 4 5
3.3 DO PLANTRPRODUCED CPP-FUSION PROTEINS PENETRATE I N T O PROTOPLASTS
AND H U M A N CELLS? ..47
3.4 DO CPP-PAH FUSION PROTEINS EXPRESSED IN PLASTIDS EXERT A POSITIVE
EFFECT IN VIVO ? 5 0
4 SUMMARY 53
5 ZUSAMMENFASSUNG 55
6 MATERIAL AND METHODS 57
6.1 MATERIAL 57
6.1.1 CHEMICALS AND ENZYMES 57
6.1.2 KITS, CONSUMABLES, EQUIPMENT AND SOFTWARE 5 8
6.1.3 DNA AND ORGANISMS 59
6.2 M E T H O D S 6 1
6.2.1 VECTOR CLONING 6 1
6.2.2 TRANSFORMATION 6 1
6.2.2.1 E. COLI 6 1
6.2.2.2 TOBACCO 6 2
6.2.3 TRANSGENIC PLANTS 6 8
6.2.3.1 TRANSPLASTOMIC LINES 6 8
6.2.3.2 NUCLEAR TRANSFORMANTS 6 8
6.2.4 MOLECULAR ANALYSIS 6 9
6.2.4.1 DNA ISOLATION F R O M PLANT TISSUE 6 9
6.2.4.2 POLYMERASE CHAIN REACTION 7 0
HTTP://D-NB.INFO/1025170210
IMAGE 2
6.2.4.3 SOUTHERN B L O T ANALYSIS 7 1
6.2.5 MACROSCOPIC ANALYSIS 7 2
6.2.5.1 VEGETATIVE AND REPRODUCTIVE G R O W T H 7 2
6.2.5.2 SEED ASSAY 7 2
6.2.6 MICROSCOPIC ANALYSIS 7 2
6.2.6.1 FLUORESCENCE MICROSCOPY 7 2
6.2.6.2 CONFOCAL LASER SCANNING MICROSCOPY (CLSM) 7 2
6.2.7 CHROMATOGRAPHIC ANALYSIS 7 3
6.2.8 BIOCHEMICAL ANALYSIS 73
6.2.8.1 EXTRACTION O F T O T A L SOLUBLE PROTEIN (TSP) 7 3
6.2.8.2 THE BRADFORD ASSAY 7 4
6.2.8.3 SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS
(SDS-PAGE) 7 4
6.2.8.4 ISOLATION O F CPP FUSION PROTEINS 7 4
6.2.8.5 WESTERN B L O T 7 5
6.2.9 FUNCTIONAL FUSION PROTEIN ASSAYS 7 5
6.2.9.1 TRANSDUCTION O F CPP FUSION PROTEINS I N T O PLANT CELLS 7 5
6.2.9.2 TRANSDUCTION O F CPP FUSION PROTEINS INTO HUMAN CELLS 7 6
7 ABBREVIATIONS 77
8 TABLES AND FIGURES 79
9 REFERENCES 8 1
10 ACKNOWLEDGEMENTS 95
11 CURRICULUM VITAE 97
12 ERKLARUNG 99
|
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| indexdate | 2024-12-20T16:13:50Z |
| institution | BVB |
| language | English |
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| physical | 94 S. Ill., graph. Darst. |
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| spellingShingle | Mayer, Florian The expression of CPP fusion proteins in plastids |
| subject_GND | (DE-588)4113937-9 |
| title | The expression of CPP fusion proteins in plastids |
| title_auth | The expression of CPP fusion proteins in plastids |
| title_exact_search | The expression of CPP fusion proteins in plastids |
| title_full | The expression of CPP fusion proteins in plastids Florian Mayer |
| title_fullStr | The expression of CPP fusion proteins in plastids Florian Mayer |
| title_full_unstemmed | The expression of CPP fusion proteins in plastids Florian Mayer |
| title_short | The expression of CPP fusion proteins in plastids |
| title_sort | the expression of cpp fusion proteins in plastids |
| topic_facet | Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/14521/ http://d-nb.info/1024457389/34 https://nbn-resolving.org/urn:nbn:de:bvb:19-145219 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025237959&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mayerflorian theexpressionofcppfusionproteinsinplastids |