Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
|
| Schlagwörter: | |
| Links: | http://epub.uni-regensburg.de/25607/ https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-256071 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025220671&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | III, 85 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV040366976 | ||
| 003 | DE-604 | ||
| 005 | 20130517 | ||
| 007 | t| | ||
| 008 | 120816s2012 xx ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)812234626 | ||
| 035 | |a (DE-599)BVBBV040366976 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 | ||
| 084 | |a VW 5075 |0 (DE-625)147763: |2 rvk | ||
| 100 | 1 | |a Saugspier, Michael Sebastian |d 1981- |e Verfasser |0 (DE-588)1025160266 |4 aut | |
| 245 | 1 | 0 | |a Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |c vorgelegt von Michael Sebastian Saugspier |
| 264 | 1 | |c 2012 | |
| 300 | |a III, 85 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Regensburg, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a Cytostatikum |0 (DE-588)4068347-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Bitterstoff |0 (DE-588)4202498-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Astrozyt |0 (DE-588)4143257-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Leber |0 (DE-588)4034933-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Hopfen |g Art |0 (DE-588)4160640-1 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Leber |0 (DE-588)4034933-0 |D s |
| 689 | 0 | 1 | |a Astrozyt |0 (DE-588)4143257-5 |D s |
| 689 | 0 | 2 | |a Hopfen |g Art |0 (DE-588)4160640-1 |D s |
| 689 | 0 | 3 | |a Bitterstoff |0 (DE-588)4202498-5 |D s |
| 689 | 0 | 4 | |a Cytostatikum |0 (DE-588)4068347-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:355-epub-256071 |
| 856 | 4 | 1 | |u http://epub.uni-regensburg.de/25607/ |x Verlag |z kostenfrei |3 Volltext |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-256071 |x Resolving-System | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025220671&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-025220671 | |
Datensatz im Suchindex
| DE-BY-TUM_katkey | 1870582 |
|---|---|
| _version_ | 1823626515793838080 |
| adam_text |
IMAGE 1
I
TABLE OF CONTENTS
1 SUMMARY 1
2 INTRODUCTION 3
2.1 BOTANY 3
2.2 HOP CHEMISTRY 5
2.2.1 LUPULIN INGREDIENTS 5
2.2.2 HOP BITTER ACIDS 6
2.3 BIOLOGICAL EFFECTS OF HOP BITTER ACIDS 8
2.3.1 ANTI-BACTERIAL EFFECTS 8
2.3.2 ANTI-INFLAMMATORY EFFECTS 9
2.3.3 ANTI-CANCER EFFECTS 12
2.3.4 EFFECTS ON LIPID AND CARBOHYDRATE METABOLISM 14
2.3.5 OTHER EFFECTS 16
2.4 TOXICOLOGY 16
2.5 EFFECTS OF HOP BITTER ACIDS ON THE LIVER 18
2.6 LIVER DISEASES 20
2.6.1 DEFINITION AND DEVELOPMENT 20
2.6.2 LIVER FIBROSIS 20
2.6.3 LIVER CANCER 21
2.7 AIM OF THE THESIS 23
3 MATERIALS AND METHODS 24
3.1 CHEMICALS AND REAGENTS 24
3.2 LABORATORY EXPENDABLES 25
3.3 LABORATORY INSTRUMENTS 25
3.4 CELL CULTURE 26
3.4.1 CELL CULTURE MEDIUM 26
3.4.2 CULTIVATION OF CELLS 27
3.4.3 DETERMINATION OF CELL NUMBER AND VIABILITY 27
3.4.4 CRYOPRESERVATION OF CELLS 28
3.4.5 HUMAN HEPATOCELLULAR CARCINOMA CELL LINES 28
3.4.6 ISOLATION OF PRIMARY HUMAN HEPATIC STELLATE CELLS 28
3.4.7 ISOLATION OF PRIMARY MURINE HEPATIC STELLATE CELLS 29
3.5 HOP BITTER ACID EXTRACTS 31
HTTP://D-NB.INFO/1026354757
IMAGE 2
TABLE OF CONTENTS LI
3.6 ENRICHMENT OF HOP BITTER ACIDS 32
3.7 PREPARATION OF A HOP BITTER ACID-SUPPLEMENTED CHOW FOR ORAL
APPLICATION 32
3.8 HOP BITTER ACID-RECOVERY FROM THE HOP BITTER ACID-SUPPLEMENTED
CHOW. 33
3.9 THIN LAYER CHROMATOGRAPHY 33
3.10 RNA ANALYSIS 34
3.10.1 RNA ISOLATION 34
3.10.2 REVERSE TRANSCRIPTION PCR 34
3.10.3 QUANTITATIVE REAL TIME PCR 35
3.11 PROTEIN ANALYSIS 36
3.11.1 ISOLATION OF WHOLE CELL PROTEINS 36
3.11.2 DETERMINATION OF PROTEIN CONCENTRATION 37
3.11.3 WESTERN BLOT ANALYSIS 37
3.11.3.1 SDS PAGE GEL ELECTROPHORESIS 37
3.11.3.2 PROTEIN BLOT AND DETECTION 38
3.11.4 IMMUNOCYTOCHEMISTRY 40
3 . 1 1 . 5 QUANTIFICATION OF E R K 1 / 2 AND N F K B 4 0
3.11.6 AP-1 REPORTER GENE ASSAY 41
3.12 ANALYSIS OF CELL CULTURE SUPERNATANTS 41
3.13 FUNCTIONAL ASSAYS 42
3.13.1 PROLIFERATION ASSAY (XTT) 42
3.13.2 MIGRATION ASSAY 42
3.14 STATISTICAL ANALYSIS 43
4 RESULTS 44 -I
4.1 EFFECTS OF HOP BITTER ACIDS ON HEPATIC STELLATE CELLS 44
4.1.1 RATIONALE 44
4.1.2 IN VITRO ACTIVATION OF HSC 45
4.1.3 PROLIFERATION AND CELL VIABILITY 46
4 . 1 . 4 N F K B ACTIVITY AND PRO-INFLAMMATORY GENE EXPRESSION 4 9
4.1.5 SUMMARY 50
4.2 EFFECTS OF HOP BITTER ACIDS ON HEPATOCELLULAR CARCINOMA CELLS 51
4.2.1 RATIONALE 51
4.2.2 CELL VIABILITY 51
4.2.3 PROLIFERATION AND MIGRATORY POTENTIAL 53
4 . 2 . 4 E R K 1 / 2 , A P - 1 AND N F K B ACTIVITY 5 4
IMAGE 3
TABLE OF CONTENTS III
4.2.5 COMPARISON OF BITTER ACIDS AND REMNANT LIPOPHILIC COMPOUNDS 57
4.2.6 SUMMARY 57
4.3 BA-RECOVERY EXPERIMENTS FROM RODENT CHOW SUPPLEMENTED BA-EXTRACT. 58
5 DISCUSSION 61
5.1 HOP BITTER ACIDS AND HEPATIC INFLAMMATION AND FIBROSIS 61
5.2 HOP BITTER ACIDS AND HEPATOCELLULAR CARCINOMA 63
5.3 POOR RECOVERY OF HOP BITTER ACIDS IN ANIMAL CHOW SUPPLEMENTED WITH A
BITTER ACID-EXTRACT 64
5.4 CONCLUSION 66
6 REFERENCES 68
7 ABBREVIATIONS 77
8 APPENDIX 80
8.1 CURRICULUM VITAE 80
8.2 ADVANCED TRAINING COURSES 81
8.3 PUBLICATIONS 81
8.4 POSTER PRESENTATIONS 82
8.5 DANKSAGUNG 83
8.6 EIDESSTATTLICHE ERKIARUNG 85 |
| any_adam_object | 1 |
| author | Saugspier, Michael Sebastian 1981- |
| author_GND | (DE-588)1025160266 |
| author_facet | Saugspier, Michael Sebastian 1981- |
| author_role | aut |
| author_sort | Saugspier, Michael Sebastian 1981- |
| author_variant | m s s ms mss |
| building | Verbundindex |
| bvnumber | BV040366976 |
| classification_rvk | VW 5075 |
| collection | ebook |
| ctrlnum | (OCoLC)812234626 (DE-599)BVBBV040366976 |
| discipline | Chemie / Pharmazie |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV040366976</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20130517</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">120816s2012 xx ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)812234626</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV040366976</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">VW 5075</subfield><subfield code="0">(DE-625)147763:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Saugspier, Michael Sebastian</subfield><subfield code="d">1981-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1025160266</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells</subfield><subfield code="c">vorgelegt von Michael Sebastian Saugspier</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">III, 85 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Regensburg, Univ., Diss., 2012</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Cytostatikum</subfield><subfield code="0">(DE-588)4068347-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Bitterstoff</subfield><subfield code="0">(DE-588)4202498-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Astrozyt</subfield><subfield code="0">(DE-588)4143257-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Leber</subfield><subfield code="0">(DE-588)4034933-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Hopfen</subfield><subfield code="g">Art</subfield><subfield code="0">(DE-588)4160640-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Leber</subfield><subfield code="0">(DE-588)4034933-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Astrozyt</subfield><subfield code="0">(DE-588)4143257-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Hopfen</subfield><subfield code="g">Art</subfield><subfield code="0">(DE-588)4160640-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Bitterstoff</subfield><subfield code="0">(DE-588)4202498-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="4"><subfield code="a">Cytostatikum</subfield><subfield code="0">(DE-588)4068347-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:355-epub-256071</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://epub.uni-regensburg.de/25607/</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-256071</subfield><subfield code="x">Resolving-System</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025220671&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025220671</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040366976 |
| illustrated | Illustrated |
| indexdate | 2025-02-09T19:00:40Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025220671 |
| oclc_num | 812234626 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| physical | III, 85 S. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spellingShingle | Saugspier, Michael Sebastian 1981- Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells Cytostatikum (DE-588)4068347-3 gnd Bitterstoff (DE-588)4202498-5 gnd Astrozyt (DE-588)4143257-5 gnd Leber (DE-588)4034933-0 gnd Hopfen Art (DE-588)4160640-1 gnd |
| subject_GND | (DE-588)4068347-3 (DE-588)4202498-5 (DE-588)4143257-5 (DE-588)4034933-0 (DE-588)4160640-1 (DE-588)4113937-9 |
| title | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |
| title_auth | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |
| title_exact_search | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |
| title_full | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells vorgelegt von Michael Sebastian Saugspier |
| title_fullStr | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells vorgelegt von Michael Sebastian Saugspier |
| title_full_unstemmed | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells vorgelegt von Michael Sebastian Saugspier |
| title_short | Analysis of the anti-fibrotic effects of hop bitter acids on hepatic stellate cells and their anti-tumorigenic effects on hepatocellular carcinoma cells |
| title_sort | analysis of the anti fibrotic effects of hop bitter acids on hepatic stellate cells and their anti tumorigenic effects on hepatocellular carcinoma cells |
| topic | Cytostatikum (DE-588)4068347-3 gnd Bitterstoff (DE-588)4202498-5 gnd Astrozyt (DE-588)4143257-5 gnd Leber (DE-588)4034933-0 gnd Hopfen Art (DE-588)4160640-1 gnd |
| topic_facet | Cytostatikum Bitterstoff Astrozyt Leber Hopfen Art Hochschulschrift |
| url | http://epub.uni-regensburg.de/25607/ https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-256071 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025220671&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT saugspiermichaelsebastian analysisoftheantifibroticeffectsofhopbitteracidsonhepaticstellatecellsandtheirantitumorigeniceffectsonhepatocellularcarcinomacells |