Analysis of functional domains of Par-4:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Jülich
Forschungszentrum Jülich
2012
|
| Schriftenreihe: | Schriften des Forschungszentrums Jülich
Reihe Gesundheit ; Bd. 50 |
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024952509&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 85 S. graph. Darst. 240 mm x 170 mm |
| ISBN: | 9783893367665 |
Internformat
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| 100 | 1 | |a Tiruttani Subhramanyam, Udaya Kumar |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Analysis of functional domains of Par-4 |c U.K. Tiruttani Subhramanyam |
| 264 | 1 | |a Jülich |b Forschungszentrum Jülich |c 2012 | |
| 300 | |a 85 S. |b graph. Darst. |c 240 mm x 170 mm | ||
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| 490 | 1 | |a Schriften des Forschungszentrums Jülich : Reihe Gesundheit |v Bd. 50 | |
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Datensatz im Suchindex
| _version_ | 1819362164713979904 |
|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS
L I S T O F F I G U R E S 7
L I S T O F T A B L E S 9
1 I N T R O D U C T I O N 1 0
2 M A T E R I A L S A N D M E T H O D S 1 5
2 . 1 M A T E R I A L S A N D T H E I R S O U R C E 1 5
2 . 1 . 1 K I T S : 1 5
2 . 1 . 2 M O L E C U L A R BIOLOGY MATERIALS-. 1 5
2 . 1 . 3 R E S I N S A N D COLUMNS: 1 5
2 . 1 . 4 I N S T R U M E N T S : 1 5
2 . 1 . 5 B A C T E R I A L STRAINS, E X P R E S S I O N VECTORS A N D
PLASMIDS: 1 6
2 . 1 . 6 M I S C E L L A N E O U S MATERIALS: 1 7
2 . 2 C L O N I N G 1 8
2 . 2 . 1 S I T E - D I R E C T E D MUTAGENESIS 1 9
2 . 3 O V E R E X P R E S S I O N 2 0
2 . 4 P R O T E I N P U R I F I C A T I O N 2 1
2 . 4 . 1 A F F I N I T Y CHROMATOGRAPHY 2 1
2 . 4 . 2 I O N - E X C H A N G E CHROMATOGRAPHY 2 1
2 . 4 . 3 SIZE EXCLUSION CHROMATOGRAPHY 2 1
2 . 4 . 4 P R O T E I N ESTIMATION A N D CONCENTRATION 2 3
2 . 5 P R O T E I N LABELING 2 3
2 . 5 . 1 S E L E N O M E T H I O N I N E INCORPORATION INTO COILED COIL
D O M A I N 2 3
2 . 5 . 2 H E A V Y A T O M DERIVATIZATION 2 4
2 . 5 . 3 I S O T O P F C L A B E L I N G ( 1 5 N ) OF S A C A N D S A C
- T 1 5 5 E 2 4
2 . 6 C I R C U L A R DICHROISM 2 4
2 . 7 FLUORESCENCE S P E C T R O S C O P Y 2 5
2 . 8 D Y N A M I C LIGHT S C A T T E R I N G 2 7
2 . 9 N M R E X P E R I M E N T 2 7
2 . 1 0 X - R A Y CRYSTALLOGRAPHY 2 8
2 . 1 0 . 1 CRYSTALLIZATION BY V A P O R DIFFUSION 2 8
2 . 1 0 . 2 CRYO-PROTECTION A N D CRYO-COOLING OF CRYSTALS 2 8
4
HTTP://D-NB.INFO/1021275921
IMAGE 2
2 . 1 0 . 3 D A T A COLLECTION A N D D A T A PROCESSING 2 9
2 . 1 0 . 4 P H A S E PROBLEM A N D M A D PHASING 3 0
3 R E S U L T S 3 3
3 . 1 E X P R E S S I O N A N D P U R I F I C A T I O N O F COILED COIL
D O M A I N 3 3
3 . 2 P U R I F I C A T I O N O F S E M E T - C C D O M A I N 3 4
3 . 3 CLONING, E X P R E S S I O N A N D P U R I F I C A T I O N O F S A
C D O M A I N A N D ITS P H O S P H O R Y L A T I O N
MIMICKING M U T A N T S 3 5
3 . 4 D Y N A M I C LIGHT S C A T T E R I N G 3 8
3 . 5 FLUORESCENCE S P E C T R O S C O P Y 3 9
3 . 6 C I R C U L A R DICHROISM 4 0
3 . 7 N M R S P E C T R O S C O P Y 4 2
3 . 8 CRYSTALLIZATION O F C C D O M A I N 4 3
3 . 9 CRYSTALLIZATION E X P E R I M E N T S W I T H S A C D O M A I N 4
6
3 . 1 0 D A T A COLLECTION A N D PROCESSING 4 8
3 . 1 1 E X P E R I M E N T A L P H A S I N G W I T H S H E L X C / D /
E 5 1
3 . 1 2 M O D E L R E F I N E M E N T 5 4
3 . 1 3 LEUCINE Z I P P E R D O M A I N 5 6
3 . 1 4 L E F T H A N D E D SUPERCOIL O F RIGHT H A N D E D HELIX 5 7
4 D I S C U S S I O N 5 9
4 . 1 CRYSTALLIZATION O F C C D O M A I N 5 9
4 . 2 COILED COIL D O M A I N W I T H LEUCINE Z I P P E R D O M A I N 5
9
4 . 3 S P A C E G R O U P D E T E R M I N A T I O N 6 0
4 . 4 P U R I F I C A T I O N A N D CRYSTALLIZATION O F S A C D O M A I
N 6 2
4 . 5 S A C D O M A I N P H O S P H O R Y L A T I O N MIMICKING M U T A
N T S 6 2
4 . 6 P H O S P H O R Y L A T I O N D E P E N D E N T SIGNALING 6 3
4 . 7 T H E POSSIBLE ROLE O F OLIGOMERIZATION 6 5
5 S U M M A R Y 6 6
A P P E N D I X I: P C R C O M P O S I T I O N 6 7
A P P E N D I X I I : D N A A N D P R O T E I N S E Q U E N C E S 6 8
5
IMAGE 3
P A R - 4 ( W I T H O U T T A G ) D N A SEQUENCE 6 8
P A R - 4 ( W I T H O U T T A G ) PROTEIN SEQUENCE 6 8
C C ( W I T H STREP T A G ) D N A SEQUENCE 6 9
C C ( W I T H S T R E P T A G ) PROTEIN SEQUENCE 6 9
L S A C ( W I T H O U T T A G ) D N A SEQUENCE 6 9
L S A C ( W I T H STREP T A G ) PROTEIN S E Q U E N C E 6 9
S A C ( W I T H S T R E P T A G ) D N A SEQUENCE 7 0
S A C ( W I T H S T R E P T A G ) PROTEIN S E Q U E N C E 7 0
A P P E N D I X I I I : O L I G O N U C L E O T I D E S E Q U E N C E S
7 1
P R I M E R S U S E D IN S A C & L S A C D O M A I N CLONING ( 5 - 3
) 7 1
C O M P L E M E N T A R Y PRIMERS FOR S I T E - D I R E C T E D M U T A
G E N E S I S 7 2
OLIGONUCLEOTIDES U S E D FOR CRYSTALLIZATION W I T H S A C D O M A I N 7
2
A P P E N D I X I V : N U T R I E N T M E D I A C O M P O S I T I O N 7
3
D Y T MEDIUM: 7 3
M 9 M I N I M A L MEDIUM W I T H S U P P L E M E N T S : 7 3
V I T A M I N - C O C K T A I L S T O C K SOLUTION: 7 3
S E M E T MINIMAL M E D I U M 7 3
A P P E N D I X V : S P A C E G R O U P S 7 4
B I B L I O G R A P G Y 7 6
A K N O W L E D G E M E N T S 8 2
6
|
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| author | Tiruttani Subhramanyam, Udaya Kumar |
| author_facet | Tiruttani Subhramanyam, Udaya Kumar |
| author_role | aut |
| author_sort | Tiruttani Subhramanyam, Udaya Kumar |
| author_variant | s u k t suk sukt |
| building | Verbundindex |
| bvnumber | BV040095840 |
| ctrlnum | (OCoLC)796200918 (DE-599)DNB1021275921 |
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| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.936 |
| dewey-search | 571.936 |
| dewey-sort | 3571.936 |
| dewey-tens | 570 - Biology |
| discipline | Allgemeine Naturwissenschaft Biologie |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV040095840 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T16:07:56Z |
| institution | BVB |
| isbn | 9783893367665 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024952509 |
| oclc_num | 796200918 |
| open_access_boolean | |
| owner | DE-12 |
| owner_facet | DE-12 |
| physical | 85 S. graph. Darst. 240 mm x 170 mm |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Forschungszentrum Jülich |
| record_format | marc |
| series | Schriften des Forschungszentrums Jülich |
| series2 | Schriften des Forschungszentrums Jülich : Reihe Gesundheit |
| spellingShingle | Tiruttani Subhramanyam, Udaya Kumar Analysis of functional domains of Par-4 Schriften des Forschungszentrums Jülich |
| subject_GND | (DE-588)4113937-9 |
| title | Analysis of functional domains of Par-4 |
| title_auth | Analysis of functional domains of Par-4 |
| title_exact_search | Analysis of functional domains of Par-4 |
| title_full | Analysis of functional domains of Par-4 U.K. Tiruttani Subhramanyam |
| title_fullStr | Analysis of functional domains of Par-4 U.K. Tiruttani Subhramanyam |
| title_full_unstemmed | Analysis of functional domains of Par-4 U.K. Tiruttani Subhramanyam |
| title_short | Analysis of functional domains of Par-4 |
| title_sort | analysis of functional domains of par 4 |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024952509&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035800060 |
| work_keys_str_mv | AT tiruttanisubhramanyamudayakumar analysisoffunctionaldomainsofpar4 |