Regulation and function of host cell miRNAs upon Helicobacter pylori infection:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Berlin
Mensch & Buch Verl.
2011
|
| Schriftenreihe: | Biological science
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024714613&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | III, 109 S. Ill., graph. Darst. |
| ISBN: | 9783863870836 |
Internformat
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| 245 | 1 | 0 | |a Regulation and function of host cell miRNAs upon Helicobacter pylori infection |c Manuel Koch |
| 264 | 1 | |a Berlin |b Mensch & Buch Verl. |c 2011 | |
| 300 | |a III, 109 S. |b Ill., graph. Darst. | ||
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| 502 | |a Zugl.: Berlin, Humboldt-Univ., Diss., 2011 | ||
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Datensatz im Suchindex
| _version_ | 1819377390598488064 |
|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS
ABSTRACT 1
ZUSAMMENFASSUNG 2
1. INTRODUCTION 3
1.1. THE SPECIES HELICOBACTER PYLORI 3
1.2. THE DEEP RELATION BETWEEN H. PYLORI AND HUMANS 3
1.3. THE HUMAN HOST 4
1.4. H. PYLORI ASSOCIATED DISEASES 5
1.5. H. PYLORI VIRULENCE FACTORS 8
1.5.1. VIRULENCE FACTORS FOR THE ACUTE INFECTION 8
1.5.2. VIRULENCE FACTORS TO ESTABLISH A CHRONIC INFECTION 9
1.5.3. THE CAG PATHOGENICITY ISLAND (CAGPAI) 10
1.6. THE HOST CELL RESPONSE 10
1.6.1. H. PYLORI INDUCED GASTRIC EPITHELIAL CELL RESPONSE 10
1.6.2. PROFESSIONAL PHAGOCYTES INFILTRATING THE GASTRIC TISSUE UPON H.
PYLORI INFECTION .... 13
1.6.3. THE ADAPTIVE IMMUNE RESPONSE DURING H. PYLORI INFECTION 14
1.7. THE PRO-INFLAMMATORY INNATE IMMUNE RESPONSE TO H. PYLORI 15
1.7.1. GENERAL ASPECTS O F THE INNATE IMMUNE RESPONSE 15
1.7.2. THE DETECTION O F H. PYLORI IN GASTRIC EPITHELIAL CELLS 18
1.7.3. THE DETECTION O F H. PYLORI IN PROFESSIONAL PHAGOCYTES 19
1.8. THE FUNCTIONS O F MICRORNAS IN MAMMALIAN CELLS 20
1.8.1. MIRNAS IN THE PROCESS O F INFECTION 20
1.8.2. DOWNSTREAM FUNCTION O F MIR-155 21
1.9. GENERAL ASPECTS O F APOPTOSIS 22
1.10. A I M O F THE STUDY 23
2. MATERIALS AND METHODS 24
2.1. CELL LINES, PRIMARY CELLS, GASTRIC TISSUE 24
2.1.1. CULTIVATION OF CELL LINES 2 4
2.1.2. CULTURE AND GENERATION O F PRIMARY BONE MARROW DERIVED
MACROPHAGES (BMMS).. 24
2.1.3. GENERATION O F L929 SUPERNATANT 24
2.1.4. HUMAN SAMPLE GENERATION 25
2.2. BACTERIA AND INFECTION EXPERIMENTS 26
I
HTTP://D-NB.INFO/1018882995
IMAGE 2
2.2.1. BACTERIAL CULTURE 2 6
2.2.2. INFECTION EXPERIMENTS 26
2.3. CHEMICALS, LIGANDS, AND INHIBITORS 26
2.3.1. CHEMICALS 26
2.3.2. H. PYLORI EFFECTORS 27
2.3.3. ACTIVATORS AND INHIBITORS 27
2.4. RNA TECHNIQUES 2 7
2.4.1. RNA ISOLATION 27
2.4.2. QRT-PCR 28
2.4.3. NORTHERN BLOT 28
2.4.4. MICROARRAY ANALYSIS 29
2.5. PROTEIN TECHNIQUES 29
2.5.1. WESTERN BLOT AND QUANTIFICATION 29
2.5.2. FACS ANALYSIS 3 0
2.5.3. IMMUNOCYTOCHEMISTRY (ICC) 30
2.6. LUCIFERASE ASSAY FOR TARGET DETERMINATION 31
2.7. APOPTOSIS ASSAYS 32
2.8. MICE 32
2.9. IN VIVO ANALYSIS 32
2.10. ANALYSIS O F DATA 33
2.10.1. TARGET PREDICTION AND PATHWAY ANALYSIS 33
2.10.2. SOFTWARE TOOLS 33
2.10.3. STATISTICAL ANALYSIS 34
3. RESULTS 35
3.1. IDENTIFICATION O F DIFFERENTIALLY REGULATED MIRNAS UPON H. PYLORI
INFECTION IN DIFFERENT CELL LINES 35
3.2. VALIDATION OF MIRNA MICROARRAY RESULTS 37
3.3. REGULATION O F MIR-155 IN H. PYLORI INFECTED GASTRIC TISSUE AND
PRIMARY CELLS 38
3.4. PRIMARY MURINE BONE MARROW DERIVED MACROPHAGES (BMMS) AS A MODEL
SYSTEM 42
3.5. REGULATION O F IL-6 IN H. PYLORI INFECTED B M M S 4 3
3.6. TLR DEPENDENT REGULATION O F MIR-155 IN B M M S 45
3.7. TLR INDEPENDENT, BUT T4SS DEPENDENT REGULATION O F MIR-155 IN B M M
S 49
3.8. DOWNSTREAM EFFECTS O F MIR-155 UPON H. PYLORI INFECTION 54
I I
IMAGE 3
3.9. STRONG ENRICHMENT OF DIRECT MIR-155 TARGETS UPON H. PYLORI
INFECTION 55
3.10. VALIDATION O F DIRECT TARGETS O F MIR-155 56
3.11. ANTI-APOPTOTIC FUNCTION O F MIR-155 UPON H. PYLORI INFECTION 61
3.12. IN VIVO FUNCTIONS O F MIR-155 UPON H. PYLORI INFECTION 62
4. DISCUSSION 68
REFERENCES 86
APPENDIX 102
METHOD TABLES 102
NORTHERN BLOT 102
WESTERN BLOT 102
ANTIBODY TABLE 104
PRIMER TABLE 105
ABBREVIATIONS 106
ACKNOWLEDGEMENTS 107
CURRICULUM VITAE 108
SELBSTSTANDIGKEITSERKLARUNG 109
I I I
|
| any_adam_object | 1 |
| author | Koch, Manuel 1981- |
| author_GND | (DE-588)1019346744 |
| author_facet | Koch, Manuel 1981- |
| author_role | aut |
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| building | Verbundindex |
| bvnumber | BV039854946 |
| classification_rvk | XD 6000 |
| ctrlnum | (OCoLC)796188075 (DE-599)DNB1018882995 |
| dewey-full | 616.33014 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.33014 |
| dewey-search | 616.33014 |
| dewey-sort | 3616.33014 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV039854946 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T16:03:52Z |
| institution | BVB |
| isbn | 9783863870836 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024714613 |
| oclc_num | 796188075 |
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| physical | III, 109 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Mensch & Buch Verl. |
| record_format | marc |
| series2 | Biological science |
| spellingShingle | Koch, Manuel 1981- Regulation and function of host cell miRNAs upon Helicobacter pylori infection Apoptosis (DE-588)4291096-1 gnd Genexpression (DE-588)4020136-3 gnd Helicobacter pylori (DE-588)4319396-1 gnd miRNS (DE-588)7736808-3 gnd Helicobacter-pylori-Infektion (DE-588)4206296-2 gnd |
| subject_GND | (DE-588)4291096-1 (DE-588)4020136-3 (DE-588)4319396-1 (DE-588)7736808-3 (DE-588)4206296-2 (DE-588)4113937-9 |
| title | Regulation and function of host cell miRNAs upon Helicobacter pylori infection |
| title_auth | Regulation and function of host cell miRNAs upon Helicobacter pylori infection |
| title_exact_search | Regulation and function of host cell miRNAs upon Helicobacter pylori infection |
| title_full | Regulation and function of host cell miRNAs upon Helicobacter pylori infection Manuel Koch |
| title_fullStr | Regulation and function of host cell miRNAs upon Helicobacter pylori infection Manuel Koch |
| title_full_unstemmed | Regulation and function of host cell miRNAs upon Helicobacter pylori infection Manuel Koch |
| title_short | Regulation and function of host cell miRNAs upon Helicobacter pylori infection |
| title_sort | regulation and function of host cell mirnas upon helicobacter pylori infection |
| topic | Apoptosis (DE-588)4291096-1 gnd Genexpression (DE-588)4020136-3 gnd Helicobacter pylori (DE-588)4319396-1 gnd miRNS (DE-588)7736808-3 gnd Helicobacter-pylori-Infektion (DE-588)4206296-2 gnd |
| topic_facet | Apoptosis Genexpression Helicobacter pylori miRNS Helicobacter-pylori-Infektion Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024714613&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kochmanuel regulationandfunctionofhostcellmirnasuponhelicobacterpyloriinfection |