Neuropeptides: methods and protocols
Gespeichert in:
| Weitere beteiligte Personen: | |
|---|---|
| Format: | Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press, Springer
2011
|
| Schriftenreihe: | Methods in molecular biology
789 |
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024448752&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024448752&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | Literaturangaben |
| Umfang: | XI, 416 S. Ill., graph. Darst. |
| ISBN: | 9781617793097 |
Internformat
MARC
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| 245 | 1 | 0 | |a Neuropeptides |b methods and protocols |c ed. by Adalberto Merighi |
| 264 | 1 | |a New York, NY [u.a.] |b Humana Press, Springer |c 2011 | |
| 300 | |a XI, 416 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1819356569995837440 |
|---|---|
| adam_text | Contents
Preface
..............................................................
v
Contributors.
......................................................... ix
1
What Are Neuropeptides?
............................................ 1
J. Peter H. Burbach
2
Neuropeptide
Localization in Nonmammalian Vertebrates
................... 37
Paolo
de Girolamo
and
Carla Lucini
3
Combined Light and Electron Microscopic Visualization of Neuropeptides
and Their Receptors in Central Neurons
................................. 57
Chiara
Salio,
Laura
Lassi,
and
Adalberto
Merißhi
4
Neuropeptide RNA
Localization
in Tissue
Sections
......................... 73
Marc Landry, Shérine Abdel
Salam,
and Marie
Moftab
5
Intron-Specific
Neuropeptide
Probes
................................... 89
Harold
Gainer,
ToddA. Ponzio, Chunmei Yue, and Makoto Kawasaki
6
Direct In Situ RT-PCR
..............................................
Ill
Laura Lossi, Graziana Gambino,
Chiara
Salio,
and
Adalberto
Merißhi
7
Laser Capture
Microdissection
and Quantitative-PCR Analysis
................ 127
Sarah J. Paulsen and LeifK. Larsen
8
Visualization of
Peptide
Secretory Vesicles in Living Nerve Cells
............... 137
Joshua J. Park and Y. Peng
Loh
9
Fluorescence Imaging with Single-Molecule Sensitivity and Fluorescence
Correlation Spectroscopy of Cell-Penetrating Neuropeptides
................. 147
Viadana
Vukojević,
Astrid
Gräslund, and
Georgy Bakaikin
10
Analysis of
Neuroimmune
Interactions by an In Vitro Coculture Approach
....... 171
Tadahide Furuno and Mamoru Nakanishi
11
Electrophysiology
.................................................. 181
Zhi-Qtnß
David Xu
12
Localization of Neuropeptides by Radioimmunoassay
....................... 191
Fred Nyberß
and
Mathias
Hallberg
13
Reversed-Phase HPLC and Hyphenated Analytical Strategies
for Peptidomics
................................................... 203
Anne-Marie Hesse,
Seßa Ndiaye,
and
Joëlle Vinh
14
Neuropeptidomics: Mass Spectrometry-Based Qualitative
and Quantitative Analysis
............................................ 223
Ping Yin, Xiaowen
Hou,
Elena
V. Romanova,
and Jonathan V. Sweedler
15
Suppression Subtractive Hybridization
.................................. 237
Mohamed
T. Ghorbel
and David Murphy
VII
viii Contents
16
Neuropeptide Microdialysis
in Free-Moving Animals.......................
261
Tetsuya Kushikata and Kazuyoshi
Utrota
17
Antibody
Microprobes
for Detecting
Neuropeptide
Release
.................. 271
Rebecca
J.
Steagall, Carole A. Williams, and Arthur W.
Ducati
18
Neuropeptidases
................................................... 287
Manuel Ramirez, Isabel
Prieto, Inmaculada
Banegas,
Ana
В.
Segarra, and Francisco Alba
19
Neuropeptide
Autoantibodies
Assay....................................
295
Sergueï
O. Fetissov
20 Intranasal
Delivery of Neuropeptides
................................... 303
Michael
G
Veronesi,
Daniel
J.
Kubek,
and Michael
J.
Kubek
21
Prodrug Design for Brain Delivery of Small- and Medium-Sized
Neuropeptides
.................................................... 313
Katalin
Prokai-Tatrai and Laszlo Prokai
22
Measurement of Phosphorothioate Oligodeoxynucleotide Antisense
Transport Across the Blood-Brain Barrier
................................ 337
William A. Bank
23
Liposome-Encapsulated Neuropeptides for Site-Specific Microinjection
......... 343
Frédéric
Frézard,
Robson A.S.
dos Santos, and Marco A.P.
Fontes
24
Recombinant Adeno-Associated
Viral
Vectors
............................. 357
Marijke
W.A. de
Backer, Keith
M.
Garner, Mieneke CM. Luijendijk,
and Roger A.H. Adan
25
Deorphanizing
G
Protein-Coupled Receptors by a Calcium
Mobilization Assay
................................................. 377
Isabel Beets,
Marleen Lindemans,
Tom
Janssen, and Peter Verleyen
Index
............................................................... 393
Methods in Molecular Biology
789
John M. Walker, Series Editor
Adalberto
Merighi Editor
Neuropeptides
Methods and Protocols
Since the early days, the field ot
neuropeptide
biology has dramatically widened, and today the
ultimate frontiers in
neuropeptide
research lie in the development of pharmacologically active
compounds that are capable ofcrossing the bloodbrain barrier to exert their biological role(s) in
vivo and in the construction ot genetic vectors to be employed in gene therapy. Xcitropeptides:
Methods and Protocols presents a readily reproducible collection of established and emerging
techniques for
neuropeptide
research as contributed by expert researchers in the field. The de¬
tailed methods presented cover subjects such as immunocvtochemical localization, biochemical
characterization, functional analysis, development and production of genetic probes, and the
design ot
neuropeptide
derivatives tor cellular neurobiology as well as the potential therapeutic
applications. Written in the highly successful Methods in Molecular Biology series
tormat,
chap¬
ters include introductions to their respective topics, lists of the necessary materials and reagents,
step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to follow, Xeuropeptides: Methods and Protocols is intended for a large
audience ot scientists, including histologists, biochemists, cellular and molecular biologists, and
electrophysiologists that are currently active in the field or are willing to enter this exciting and
still expanding area of neurobiology.
|
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| dewey-sort | 3612.8042 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
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| id | DE-604.BV039597800 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T15:58:11Z |
| institution | BVB |
| isbn | 9781617793097 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024448752 |
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| physical | XI, 416 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana Press, Springer |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spellingShingle | Neuropeptides methods and protocols Methods in molecular biology Neuropeptide (DE-588)4041895-9 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| subject_GND | (DE-588)4041895-9 (DE-588)4324907-3 (DE-588)4143413-4 |
| title | Neuropeptides methods and protocols |
| title_auth | Neuropeptides methods and protocols |
| title_exact_search | Neuropeptides methods and protocols |
| title_full | Neuropeptides methods and protocols ed. by Adalberto Merighi |
| title_fullStr | Neuropeptides methods and protocols ed. by Adalberto Merighi |
| title_full_unstemmed | Neuropeptides methods and protocols ed. by Adalberto Merighi |
| title_short | Neuropeptides |
| title_sort | neuropeptides methods and protocols |
| title_sub | methods and protocols |
| topic | Neuropeptide (DE-588)4041895-9 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| topic_facet | Neuropeptide Untersuchungsmethode Aufsatzsammlung |
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