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The protein protocols handbook:

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Bibliographische Detailangaben
Weitere beteiligte Personen:	Walker, John M. 1948- (HerausgeberIn)
Format:	Buch
Sprache:	Englisch
Veröffentlicht:	New York, NY Humana Press 2009
Ausgabe:	3. ed.
Schriftenreihe:	Springer protocols handbooks
Schlagwörter:	Chemistry Techniques, Analytical > methods > Laboratory Manuals Peptides > analysis > Laboratory Manuals Protein Conformation > Laboratory Manuals Proteins > Analysis > Laboratory manuals Proteins > analysis > Laboratory Manuals Biochemische Analyse Proteine Analyse
Links:	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016738392&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Umfang:	XXXV, 1985 S. Ill., graph. Darst.
ISBN:	160327474x 9781603274746

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Datensatz im Suchindex

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adam_text	Titel: The protein protocols handbook Autor: Walker, John M Jahr: 2009 Contents Preface..............................................................................................................v Contributors...................................................................................................xxiii Part I Quantitation of Proteins 1 Protein Determination by UV Absorption................................................3 Alastair Aitken and Michèle P. Learmonth 2 The Lowry Method for Protein Quantitation............................................7 Jakob H. Waterborg 3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation..............11 John M. Walker 4 The Bradford Method For Protein Quantitation.....................................17 Nicholas J. Kruger 5 Ultrafast Protein Determinations Using Microwave Enhancement........................................................................................25 Robert E. Akins and Rocky S. Tuan 6 The Nitric Acid Method for Protein Estimation in Biological Samples............................................................................35 Scott A. Boerner, Crescent R. Isham, Yean Kit Lee, Jennifer D. Tibodeau, Scott H. Kaufmann, and Keith C. Bible 7 Quantitation of Tryptophan in Proteins..................................................47 Alastair Aitken and Michèle P. Learmonth 8 Kinetic Silver Staining of Proteins.........................................................51 Douglas D. Root and Kuan Wang 9 Quantitation of Cellular Proteins by Flow Cytometry............................55 Thomas D. Friedrich, F. Andrew Ray, Ralph L. Smith, and John M. Lehman 10 Quantitation of Cellular Proteins by Laser Scanning Cytometry.............................................................................63 Thomas D. Friedrich, Ralph L. Smith, and John M. Lehman VII viii Contents Part II Electrophoresis of Proteins and Peptides and Dectection in Gels 11 Protein Solubility in Two-Dimensional Electrophoresis: Basic Principles and Issues..................................................................73 Thierry Rabilloud 12 Mouse and Human Tissue Sample Preparation for 2-D Electrophoresis.........................................................................85 Claus Zabel and Joachim Klose 13 Plant Protein Sample Preparation for 2-DE........................................109 Sebastien Christian Carpentier, Rony Swennen, and Bart Panis 14 Preparation of Bacterial Samples for 2-D PAGE.................................121 Brian B, Vandahl, Gunna Christiansen, and Svend Birkelund 15 Preparation of Bodily Fluids for 2-D PAGE..........................................131 Anthony G. Sullivan, Henry Brzeski, Jeban Ganesalingam, and Manuel Mayr 16 Immunoaffinity Depletion of High Abundance Plasma and Serum Proteins...............................................................139 Lynn A. Echan and David W. Speicher 17 Preparation of Yeast Samples for 2-D PAGE......................................155 Joakim Norbeck 18 Membrane Protein Preparation Using Aqueous Polymer Two-Phase Systems...........................................................................159 Jens Schindler and Hans Gerd Nothwang 19 Subcellular Fractionation of Small Sample Amounts..........................165 Hans Gerd Nothwang, Isabelle Guillemin, and Jens Schindler 20 Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins.................................................................171 John M. Walker 21 SDS Polyacrylamide Gel Electrophoresis of Proteins.........................177 John M. Walker 22 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins..........187 John M. Walker 23 SDS-Polyacrylamide Gel Electrophoresis of Peptides........................193 Ralph C. Judd 24 Separation of Proteins by Blue Native Electrophoresis (BN-PAGE)................................................................201 Elke A. Dian, Joachim Rassow, and Christian Motz Contents ix 25 Separation of Proteins by Gel Electrophoresis in the Tris-Taurine-HCI System............................................................211 Sylvie Luche, Mireille Chevallet, Cécile Lelong, and Thierry Rabilloud 26 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: M-Based Separation of Proteins with Retained Native Activity............................................221 Robert E. Akins and Rocky S. Tuan 27 Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins.................................................................................239 Jakob H. Waterborg 28 Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones..........................................................................................251 Jakob H. Waterborg 29 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels....................:......................................................263 John M. Walker 30 Serial Immobilized pH Gradient Isoelectric Focusing over pH 4-9.........................................................................269 Slobodan Poznanovic, Wojciech Wozny, Helmut Zengerling, Gerhard P. Schwall, and Michael A. Cahill 31 Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-Dimensional Electrophoresis...................................279 Nick Bizlos 32 Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins Using Carrier Ampholyte pH Gradients in the First Dimension.........................................................................285 Patricia Gravel 33 Vertical Agarose Electrophoresis and Electroblotting of High-Molecular-Weight Proteins.....................................................293 Marion L. Greaser and Chad M. Warren 34 Two-Dimensional PAGE of High-Molecular-Mass Proteins.................299 Masamichi Oh-lshi and Tadakazu Maeda 35 Casting Immobilized pH Gradients.....................................................305 Elisabetta Gianazza 36 A Modified Nonequilibrium pH Gradient Electrophoresis (NEPHGE) Technique for Protein Separation.....................................323 James A. Carroll x Contents 37 Microchip Capillary Electrophoresis: Application to Peptide Analysis...........................................................329 Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte 38 Protein Separations in Microfluidic Chips...........................................361 Andrea W. Chow and Bahram Fathollahi 39 Difference Gel Electrophoresis (DIGE)...............................................379 David B. Friedman and Kathryn S. Lilley 40 Comparing 2D Electrophoretic Gels Across Internet Databases: An Open Source Application..............................409 Peter F. Lemkin, Gregory C. Thornwall, and Jai A. Evans 41 Quantification of Radiolabeled Proteins in Polyacrylamide Gels.......................................................................443 Wayne R. Springer 42 Differential ProteoTope Radioactive Quantification of Protein Abundance Ratios..............................................................449 Wojciech Wozny, Gerhard P. Schwall, Chaturvedula S. Sastri, Slobodan Poznanovic,Werner Stegmann, Christian Hunzinger*, Karlfried Groebe, and Michael A. Cahill 43 Quantification of Proteins on Polyacrylamide Gels.............................479 Bryan John Smith 44 Using SDS-PAGE and Scanning Laser Densitometry to Measure Yield and Degradation of Proteins....................................487 Aaron P. Miles and Allan Saul 45 Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red.................................................497 Joan-Ramon Daban, Salvador Bartolomé, Antonio Bermúdez, and F. Javier Alba 46 Zn2+-Reverse Staining Technique........................................................505 Carlos Fernandez-Patron 47 Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels................................................................515 Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin, and Jung-Kap Choi 48 Detection of Proteins in Polyacrylamide Gels by Silver Staining................................................................................527 Michael J. Dunn 49 Background-Free Protein Detection in Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains.....................................................................................529 Wayne F. Patton Contents xi 50 Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining......................................................................547 Michael J. Dunn 51 Detection of Glycoproteins in Gels and Blots......................................555 Nicolle H. Packer, Malcolm S. Ball, Peter L. Devine, and Wayne F. Patton 52 Staining of Glycoproteins/Proteoglycans on SDS-Gels......................569 Holger J. Moller and Jörgen H. Poulsen 53 Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain.......................................575 Fan Lin and Gary E. Wise 54 A Modified Pro-Q Diamond Staining Protocol for Phosphoprotein Detection in Polyacrylamide Gels........................579 Ganesh Kumar Agrawal and Jay J. Thelen 55 Electroelution of Proteins from Polyacrylamide Gels..........................587 Paul Jenö and Martin Horst 56 Autoradiography and Fluorography of Acrylamide Gels......................595 Antonella Circolo and Sunita Gulati 57 Proteolytic Activity Detection by Two-Dimensional Zymography........................................................................................605 Jeff Wilkesman Part III Blotting and Detection Methods 58 Protein Blotting by Electroblotting.......................................................617 Mark Page and Robin Thorpe 59 Protein Blotting by the Semidry Method..............................................621 Patricia Gravel 60 Protein Blotting by the Capillary Method.............................................631 John M. Walker 61 Western Blotting of Basic Proteins Electrophoretically Resolved on Acid-Urea-Triton-Polyacrylamide Gels...........................635 Geneviève P. Delcuve and James R. Davie 62 Immunoblotting of 2-DE Separated Proteins.......................................641 Barbara Magi and Laura Bianchi 63 High-Efficiency Blotting of High-Molecular Weight Proteins................663 Marion L. Greaser and Darl R. Swartz 64 Alkaline Phosphatase Labeling of IgG Antibody.................................673 G. Brian Wisdom xii Contents 65 ß-Galactosidase Labeling of IgG Antibody..........................................677 G. Brian Wisdom 66 Horseradish Peroxidase Labeling of IgG Antibody.............................681 G. Brian Wisdom 67 Digoxigenin Labeling of IgG Antibody.................................................685 G. Brian Wisdom 68 Conjugation of Fluorochromes to Antibodies......................................687 Su-Yau Mao 69 Coupling of Antibodies with Biotin.......................................................693 Rosaria P. Haugland and Wendy W. You 70 Preparation of Avidin Conjugates........................................................705 Rosaría P. Haugland and Mahesh K. Bhalgat 71 MDPF Staining of Proteins on Western Blots.....................................717 F. Javier Alba and Joan-Ramon Daban 72. Copper Iodide Staining of Proteins and Its Silver Enhancement............................................................................723 Douglas D. Root and Kuan Wang 73 Detection of Proteins on Blots Using Direct Blue 71...........................729 Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin, and Jung-Kap Choi 74 Detection of Proteins on Western Blots Using Colorimetrie and Radiometric Visualization of Secondary Ligands.........................................................................737 Nicholas J. Kruger 75 Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting.......................................................755 Patricia Gravel 76 A Sensitive Method to Quantitatively Detect Total Protein on Membranes after Electrophoretic Transfer Using Avidin-or Streptavidin-Biotin...............................................................771 William J. LaRochelle 77 Detection and Quantification of Proteins on Immunoblots using Enhanced Chemiluminescence.................................................779 Jennifer J. Young 78 Reutilization of Western Blots After Chemiluminescent or Autoradiographic Detection.............................................................789 Scott H. Kaufmann 79 The Use of Quantum Dot Luminescent Probes for Western Blot Analysis....................................................................807 Savvas C. Makrides, Christina Gasbarro, and Job M. Bello Contents xiii 80 The Use of Infrared Fluorescent Dyes in Quantitative Immunoblotting............................................................879 Ching-Hui Yang, Christopher Kasbek, and Harold A. Fisk 81 The Use of Infrared Fluorescent Dyes in Immunofluorescence Microscopy....................................................827 Christopher Kasbek, Ching-Hui Yang, and Harold A. Fisk Part IV Chemical Modification of Proteins and Peptide Production and Purification 82 Carboxymethylation of Cysteine Using lodoacetamide/lodoacetic Acid.................................................837 Alastair Aitken and Michèle Learmonth 83 Performic Acid Oxidation.....................................................................841 Alastair Aitken and Michèle Learmonth 84 Succinylation of Proteins.....................................................................845 Alastair Aitken and Michèle Learmonth 85 Pyridylethylation of Cysteine Residues...............................................847 Malcolm Ward 86 Side Chain Selective Chemical Modifications of Proteins...................851 Dan S. Tawfik 87 Nitration of Tyrosines...........................................................................855 Dan S. Tawfik 88 Ethoxyformylation of Histidine.............................................................859 Dan S. Tawfik 89 Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal...............................................................863 Dan S. Tawfik 90 Amidation of Carboxyl Groups............................................................865 Dan S. Tawfik 91 Amidination of Lysine Side Chains.....................................................867 Dan S. Tawfik 92 Modification of Tryptophan with 2-Hydroxy- 5-Nitrobenzylbromide..........................................................................871 Dan S. Tawfik 93 Modification of Sulfhydryl Groups with DTNB.....................................873 Dan S. Tawfik 94 Chemical Cleavage of Proteins at Methionyl-X Peptide Bonds.....................................................................................875 Bryan John Smith xiv Contents 95 Chemical Cleavage of Proteins at Tryptophanyl-X Peptide Bonds.....................................................................................883 Bryan John Smith 96 Chemical Cleavage of Proteins at Aspartyl-X Peptide Bonds.............891 Bryan John Smith 97 Chemical Cleavage of Proteins at Cysteinyl-X Peptide Bonds.....................................................................................895 Bryan John Smith 98 Chemical Cleavage of Proteins at Asparaginyl-Glycyl Peptide Bonds.....................................................................................899 Bryan John Smith 99 Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels........................................................905 Kathryn L. Stone, Eroi E. Gulcicek, and Kenneth R. Williams 100 On-PVDF Protein Digestions for N-terminal Sequencing and Peptide Mass Fingerprinting........................................................919 Victoria Pham, William Henzel, and Jennie R. Lili 101 Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis........................927 Joseph Fernandez and Sheenah M. Mische 102 Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins..............................................................................941 Kathryn L. Stone and Kenneth R. Williams 103 Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography.....................................951 Ralph C. Judd 104 Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis.......................................963 Ralph C. Judd 105 Peptide Mapping for Protein Characterization....................................969 Peter Hojrup 106 Production of Protein Hydrolysates Using Enzymes...........................989 John M. Walker and Patricia J. Sweeney 107 Amino Acid Analysis by Precolumn Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-i-Alanine Amide (Marfey s Reagent)..............................................................................995 Sunil Kochhar and Philipp Christen 108 Amino Acid Analysis of Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection..............................1001 Petr Jandik, Jun Cheng, and Nebojsa Avdalovic Contents xv 109 Validation of Amino Acid Analysis Methods......................................1015 Andrew J. Reason 110 Molecular Weight Estimation for Native Proteins Using High-Performance Size-Exclusion Chromatography...............................................................................1029 G. Brent Irvine 111 Detection of Disulfide-Linked Peptides by HPLC..............................1039 Alastair Aitken and Michèle Learmonth 112 Detection of Disulfide-Linked Peptides by Mass Spectrometry....................................................................................1043 Alastair Aitken and Michèle Learmonth 113 Diagonal Electrophoresis for Detecting Disulfide Bridges.................1047 Alastair Aitken and Michèle Learmonth 114 Estimation of Disulfide Bonds Using Ellman s Reagent....................1053 Alastair Aitken and Michèle Learmonth 115 Quantitation of Cysteine Residues and Disulfide Bonds by Electrophoresis.................................................................1057 Alastair Aitken and Michèle Learmonth 116 N-Terminal Sequencing of N-Terminally Modified Proteins..............................................................................1063 Roza Maria Kamp and Hisashi Hirano 117 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid.............................................................................1075 Jacek Mozdzanowski 118 Detection and Characterization of Protein Mutations by Mass Spectrometry......................................................1081 Yoshinao Wada 119 Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry.............................................................1095 Ole Norregaard Jensen and Matthias Wilm 120 Protein Identification by Peptide Mass Fingerprinting using MALDI-TOF Mass Spectrometry.............................................1117 Judith Webster and David Oxley 121 Protein Ladder Sequencing..............................................................1131 Rong Wang and Brian T. Chait 122 Sequence Analysis with WinGene/WinPep.......................................1141 Lars Hennig 123 HPLC and Mass Spectrometry of Integral Membrane Proteins...........................................................................1149 Julian P. Whitelegge xvi Contents 124 Enrichment of Serum Peptides and Analysis by MALDI-TOF Mass Spectrometry..................................................1167 YanmingAn, Habtom W. Ressom, and Radoslav Goldman 125 Computational Methods for Analysis of MALDI-TOF Spectra to Discover Peptide Serum Biomarkers...............................1175 Habtom W. Ressom, Rency S. Varghese, and Radoslav Goldman Part V Posttranslational Modifications 126 Simple Tools for Complex A/-Glycan Analysis....................................1187 Anne-Catherine Fitchette, Meriem Benchabane, Thomas Paccalet, Loïc Faye, and Véronique Gomord 127 A Lectin-Binding Assay for the Rapid Characterization of the Glycosylation of Purified Glycoproteins..................................1205 Mohammad T. Goodarzi, Angeliki Fotinopoulou, and Graham A. Turner 128 Chemical Methods of Analysis of Glycoproteins...............................1215 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 129 Monosaccharide Analysis by HPAEC...............................................1219 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 130 Monosaccharide Analysis by Gas Chromatography (GC)................1223 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 131 Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methylation Analysis.........................................1227 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 132 Sialic Acid Analysis by HPAEC-PAD.................................................1231 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 133 Chemical Release of O-Linked Oligosaccharide Chains..................1233 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 134 O-Linked Oligosaccharide Profiling by HPLC...................................1235 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 135 O-Linked Oligosaccharide Profiling by HPAEC-PAD.........................1237 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith Contents xvii 136 Release of A/-Linked Oligosaccharide Chains by Hydrazinolysis..............................................................................1239 Tsuguo Mizuochi and Elizabeth F. Hounsell 137 Enzymatic Release of O-and A/-Linked Oligosaccharide Chains....................................................................1243 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 138 A/-Linked Oligosaccharide Profiling by H PLC on Porous Graphitized Carbon (PGC)..............................................1247 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 139 A/-Linked Oligosaccharide Profiling by HPAEC-PAD.........................1249 Elizabeth F. Hounsell, Michael J. Davies, and Kevin D. Smith 140 HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the CarboPac MA1 Column.....................................................................................1253 Michael Weitzhandler, Jeffrey Rohrer, James R. Thayer, and Nebojsa Avdalovic 141 Microassay Analyses of Protein Glycosylation.................................1261 Nicky K. C. Wong, Nnennaya Kanu, Natasha Thandrayen, Geert Jan Rademaker, Christopher I. Baldwin, David V. Renouf, and Elizabeth F. Hounsell 142 Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins...................................................1273 Brian K. Brandley, John C. Klock, and Christopher M. Starr 143 HPLC Analysis of Fluorescently Labeled Glycans............................1289 Tony Merry and Sviatlana Astrautsova 144 Glycoprofiling Purified Glycoproteins Using Surface Plasmon Resonance.................................................1313 Angeliki Fotinopoulou and Graham A. Turner 145 Sequencing Heparan Sulfate Saccharides.......................................1321 Jeremy E. Turnbull 146 Analysis of Glycoprotein Heterogeneity by Capillary Electrophoresis and Mass Spectrometry..........................................1335 Andrew D. Hooker and David C. James 147 Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectins....................................1347 Kazuo Yamamoto 148 In-Gel Enzymatic Release of W-Glycans...........................................1363 David J. Harvey xviii Contents 149 Analysis of /V-ünked Glycans by Mass Spectrometry......................1371 David J. Harvey 150 MS Analysis of Protein Glycosylation...............................................1387 NobuakiTakemori, Naoka Komori, and Hiroyuki Matsumoto 151 Mapping protein A/-Glycosylation by COFRADIC.............................1395 Bart Ghesquière, Joël Vandekerckhove, and Kris Gevaert 152 Mass Spectrometric Analysis of O-Linked Glycans Released Directly from Glycoproteins in Gels Using ß-Elimination...............................................................1403 Adrian M. Taylor and Jane Thomas-Oates 153 Glycopeptide Analysis Using LC/MS and LC/MSn: Site-Specific Glycosylation Analysis of a Glycoprotein.....................7479 Satsuki Itoh, Daisuke Takakura, Nana Kawasaki, and Teruhide Yamaguchi 154 Identification of Vitamin K-Dependent Proteins Using a Gla-Specific Monoclonal Antibody.......................................1431 Karin Hansson 155 The Identification of Protein S-Nitrosocysteine.................................1451 Todd M. Greco, Sheryl L. Stamer, Daniel C. Liebler, and Harry Ischiropoulos 156 Detection of Nitrotyrosine-Containing Proteins.................................1467 Xianquan Zhan and Dominic M. Desiderio 157 Mass Spectrometric Determination of Protein Ubiquitination....................................................................................1491 Carol E. Parker, Maria Warren Hines, Viorel Mocanu, Susanna F. Greer, and Christoph H. Borchers 158 Detection of Sumoylated Proteins.....................................................1519 Ok-Kyong Park-Sarge and Kevin D. Sarge 159 Efficient Enrichment of Intact Phosphorylated Proteins by Modified Immobilized Metal-Affinity Chromatography...............................................................................1531 Anna Dubrovska 160 Analyzing Protein Phosphorylation...................................................1547 John Colyer 161 Mass Spectrometric Analysis of Protein Phosphorylation................1555 Stefan Gander, Alessio Cremonesi, Johana Chicher, Suzette Moes, and Paul Jenö Contents xix 162 Protein Microarrays for Phosphorylation Studies..............................1567 Birgit Kersten and Tanja Feilner 163 Two-Dimensional Phosphopeptide Mapping.....................................1579 Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky, and Steven F. Dowdy 164 Identification of Proteins Modified by Protein (D-Aspartyl/L-Isoaspartyl) Carboxyl Methyltransferase......................1589 Darin J. Weber and Philip N. McFadden 165 Analysis of Tyrosine-O-Sulfation.......................................................1601 Jens R. Bundgaard, Jette W. Sen, Anders H. Johnsen, and Jens F. Reh feld 166 Analysis of Protein Palmitoylation by Metabolic Radiolabeling Methods................................................1623 Katherine H. Pedone, Leah S. Bernstein, Maurine E. Under, and John R. Hepler 167 Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins: A Useful Tool for Studying Proteins Prenylation................................1637 Alberto Corsini, Christopher C. Farnsworth, Paul McGeady, Michael H. Gelb, and John A. Glomset 168 The Metabolic Labeling and Analysis of Isoprenylated Proteins..................................................................1657 Douglas A. Andres, Dean C. Crick, H. Peter Spielmann, and Charles J. Waechter Part VI Antibody Techniques 169 Antibody Production..........................................................................1679 Robert Burns 170 Production of Antibodies Using Proteins in Gel Bands.....................................................................................1687 Sally Ann Amero, Tharappel C. James, and Sarah C. R. Elgin 171 Raising Highly Specific Polyclonal Antibodies Using Biocompatible Support-Bound Antigens...........................................1693 Monique Diano and André Le Bivio 172 Production of Antisera Using Peptide Conjugates............................7705 Thomas E. Adrian 173 Small-Molecule-Protein Conjugation Procedures............................1715 Stephen Thompson xx Contents 174 The Chloramine T Method for Radiolabeling Protein........................1727 Graham S. Bailey 175 The Lactoperoxidase Method for Radiolabeling Protein...................................................................1731 Graham S. Bailey 176 The Bolton and Hunter Method for Radiolabeling Protein...................................................................1733 Graham S. Bailey 177 Preparation of 125l-Labeled Peptides and Proteins with High Specific Activity Using IODO-GEN....................................1735 J. Michael Conlon 178 Purification and Assessment of Quality of Radioiodinated Protein..................................................................1743 Graham S. Bailey 179 Purification of IgG by Precipitation with Sodium Sulfate or Ammonium Sulfate...........................................................1749 Mark Page and Robin Thorpe 180 Purification of IgG Using Caprylic Acid.............................................1753 Mark Page and Robin Thorpe 181 Purification of IgG Using DEAE-Sepharose Chromatography...............................................................................1755 Mark Page and Robin Thorpe 182 Purification of IgG Using Ion-Exchange HPLC.................................1757 Carl Dolman, Mark Page, and Robin Thorpe 183 Purification of IgG by Precipitation with Polyethylene Glycol (PEG)........................................................1759 Mark Page and Robin Thorpe 184 Purification of IgG Using Protein A or Protein G...............................1761 Mark Page and Robin Thorpe 185 Analysis and Purification of IgG Using Size-Exclusion High Performance Liquid Chromatography (SE-HPLC)....................1765 Carl Dolman and Robin Thorpe 186 Purification of IgG Using Affinity Chromatography on Antigen-Ligand Columns..................................1769 Mark Page and Robin Thorpe 187 Purification of IgG Using Thiophilic Chromatography.......................1773 Mark Page and Robin Thorpe 188 Analysis of IgG Fractions by Electrophoresis....................................1775 Mark Page and Robin Thorpe Contents xxi 189 Purification of Immunoglobulin Y (IgY) from Chicken Eggs............................................................................1779 Christopher R. Bird and Robin Thorpe 190 Affinity Purification of Immunoglobulins Using Protein A Mimetic (RAM).........................................................1783 Giorgio Fassina, Giovanna Palombo, Antonio Verdoliva, and Menotti Ruvo 191 Detection of Serological Cross-Reactions by Western Cross-Blotting................................................................1799 Peter Hammer!, Arnulf Hartl, Johannes Freund, and Josef Thalhamer 192 Enzymatic Digestion of Monoclonal Antibodies................................1811 Sarah M. Andrew 193 How to Make Bispecific Antibodies...................................................1819 Ruth R. French 194 Antigen Measurement Using ELISA.................................................1827 William Jordan 195 Enhanced Chemiluminescence Immunoassay.................................1835 Richard A. W.Stott 196 Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins.............................................................1845 Stephen Thompson 197 Determination of Epitopes by Mass Spectrometry............................1859 Christine Hager-Braun and Kenneth B. Tomer 198 Immunogen Preparation and Immunization Procedures for Rats and Mice..........................................................1873 Mark Page and Robin Thorpe 199 Making Hybridomas..........................................................................1877 Robert Burns 200 Growing Hybridomas........................................................................1887 Gary Entrican, Catherine Jepson, and David Deane 201 Mouse Hybridomas as an Entryway to Monoclonal Antibody Design and Production.......................................................1901 Eugene Mechetner 202 Potential Pitfalls in Monoclonal Antibody Development and Applications..........................................................1911 Eugene Mechetner xx/7 Contents 203 Recombinant Antibody Expression and Purification.........................1929 Achim Knappik and Ralf Brundlers 204 Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay............................................1945 Mark Page and Robin Thorpe 205 Screening Hybridoma Culture Supernatants Using ELISA...............1947 Mark Page and Robin Thorpe 206 Growth and Purification of Murine Monoclonal Antibodies......................................................................1949 Mark Page and Robin Thorpe 207 Affinity Purification Techniques for Monoclonal Antibodies.........................................................................................1951 Alexander Schwarz 208 A Rapid Method for Generating Large Numbers of High-Affinity Monoclonal Antibodies from a Single Mouse.........................................................................1961 Nguyen Thi Man and Glenn E. Morris Index............................................................................................................1975
any_adam_object	1
author2	Walker, John M. 1948-
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id	DE-604.BV035069914
illustrated	Illustrated
indexdate	2024-12-20T13:19:25Z
institution	BVB
isbn	160327474x 9781603274746
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-016738392
oclc_num	191922694
open_access_boolean
owner	DE-703 DE-91G DE-BY-TUM DE-20 DE-19 DE-BY-UBM DE-11 DE-91S DE-BY-TUM
owner_facet	DE-703 DE-91G DE-BY-TUM DE-20 DE-19 DE-BY-UBM DE-11 DE-91S DE-BY-TUM
physical	XXXV, 1985 S. Ill., graph. Darst.
publishDate	2009
publishDateSearch	2009
publishDateSort	2009
publisher	Humana Press
record_format	marc
series2	Springer protocols handbooks
spellingShingle	The protein protocols handbook Chemistry Techniques, Analytical methods Laboratory Manuals Peptides analysis Laboratory Manuals Protein Conformation Laboratory Manuals Proteins Analysis Laboratory manuals Proteins analysis Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd Proteine (DE-588)4076388-2 gnd Analyse (DE-588)4122795-5 gnd
subject_GND	(DE-588)4255721-5 (DE-588)4076388-2 (DE-588)4122795-5
title	The protein protocols handbook
title_auth	The protein protocols handbook
title_exact_search	The protein protocols handbook
title_full	The protein protocols handbook ed. by John M. Walker
title_fullStr	The protein protocols handbook ed. by John M. Walker
title_full_unstemmed	The protein protocols handbook ed. by John M. Walker
title_short	The protein protocols handbook
title_sort	the protein protocols handbook
topic	Chemistry Techniques, Analytical methods Laboratory Manuals Peptides analysis Laboratory Manuals Protein Conformation Laboratory Manuals Proteins Analysis Laboratory manuals Proteins analysis Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd Proteine (DE-588)4076388-2 gnd Analyse (DE-588)4122795-5 gnd
topic_facet	Chemistry Techniques, Analytical methods Laboratory Manuals Peptides analysis Laboratory Manuals Protein Conformation Laboratory Manuals Proteins Analysis Laboratory manuals Proteins analysis Laboratory Manuals Biochemische Analyse Proteine Analyse
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016738392&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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