Basic methods for the biochemical lab: 23 figures and 86 tables
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Berlin [u.a.]
Springer
2006
|
| Ausgabe: | 1., engl. ed. |
| Schriftenreihe: | Springer Lab Manual
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014832367&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | XVIII, 251 S. graph. Darst. 235 mm x 155 mm |
| ISBN: | 3540327851 9783540327851 |
Internformat
MARC
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|---|---|---|---|
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| 100 | 1 | |a Holtzhauer, Martin |e Verfasser |4 aut | |
| 240 | 1 | 0 | |a Biochemische Labormethoden |
| 245 | 1 | 0 | |a Basic methods for the biochemical lab |b 23 figures and 86 tables |c Martin Holtzhauer |
| 250 | |a 1., engl. ed. | ||
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 2006 | |
| 300 | |a XVIII, 251 S. |b graph. Darst. |c 235 mm x 155 mm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
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Datensatz im Suchindex
| DE-BY-TUM_call_number | 0302 CHE 810f 2006 A 6874 1002 CHE 810f 2006 A 7843 |
|---|---|
| DE-BY-TUM_katkey | 1553486 |
| DE-BY-TUM_location | 03 10 |
| DE-BY-TUM_media_number | 040030183545 040050552000 |
| _version_ | 1821933174099279874 |
| adam_text | Table of Contents
Abbreviations
1
1.1
1.1.1
1.1.1.1
1.1.1.2
1.1.1.3
1.1.1.4
of Interfering Substances
1.1.2
1.1.3
1.1.4
1.1.5
1.1.5.1
1.1.5.2
1.1.6
1.1.7
1.2
1.2.1
and Protein Separation Procedure
1.2.2
1.2.3
1.2.4
1.2.5
1.3
1.3.1
1.3.2
1.3.3
1.4
1.5
2
2.1
2.1.1
2.1.2
(NuPAGE)
Table of Contents
2.1.3
to Weber,
2.1.4
for the Separation of Low Molecular Weight Proteins
2.1.5
for Proteins and Oligopeptides in the Range
of
2.1.6
2.1.7
atpH2
2.1.8
(Native PAGE)
2.1.9
(Native PAGE)
2.1.10
2.1.11
(2D-PAGE; IEF followed by SDS-PAGE)
2.1.11.1
2.1.11.2
(Acrylamide Gradient Gel)
2.2
2.2.1
2.2.2
2.2.3
2.3
2.3.1
2.3.1.1
2.3.1.2
2.3.2
2.3.3
2.4
2.4.1
2.4.1.1
2.4.1.2
2.4.1.3
with Bismarck Brown R
2.4.1.4
2.4.1.5
2.4.1.6
2.4.2
2.4.2.1
2.4.2.2
2.4.2.3
2.4.2.4
2.4.2.5
2.4.2.6
2.4.2.7
2.4.3
Table of Contents XI
2.4.4
2.4.4.1
2.4.4.2
2.4.5
2.4.5.1
2.4.5.2
2.4.5.3
2.5
2.5.1
from Polyacrylamide Gels
2.5.2
2.5.3
(Electroblotting; Western Blot): Semi-dry Blotting
2.5.4
(Immunoblotting)
2.5.4.1
2.5.4.2
2.5.5
2.5.5.1
2.5.5.2
2.5.6
After Electrotransfer
2.5.7
2.5.8
2.5.9
2.6
2.7
3
3.1
3.1.1
(TLC
3.1.2
3.1.3
3.1.4
3.1.5
3.2
3.3
Size-Exclusion Chromatography, SEC)
3.3.1
3.3.2
3.3.3
3.3.4
3.3.5
3.3.6
by Gel Filtration ( Desalting )
3.4
3.4.1
XII
3.4.2
3.4.3 Sample Application....................................... 104
3.4.4 Elution ................................................. 105
3.4.5
3.4.6
of
3.5 Hydrophobie
3.5.1
3.5.2 Elution ................................................. 108
3.5.3 Regeneration............................................ 108
3.5.4
3.6
3.6.1
of Polysaccharide-Based Supports
3.6.1.1
3.6.2
3.6.2.1
Spacers with 2,4,6-Trinitrobenzene Sulfonic Acid
3.6.2.2
3.6.2.3
3.6.2.4
3.6.2.5
on Immobilized Antibodies (Immunoaffinity
Chromatography, IAC)
3.6.2.6
on Protein-A Supports
3.6.3
3.6.3.1
3.6.4
3.6.5
3.6.6
Chromatography)
3.6.7
(Biotin-Avidin/Streptavidin System)
3.6.8
of Proteins Containing His6-Tag
3.7
3.7.1
3.7.2
3.7.3
3.7.4
3.7.5 Ultrafiltration............................................ 127
4
4.1
4.1.1
with Additional Free SH Groups
4.1.2
Table of
4.1.3
4-(N-Maleimidomethyl)-Cyclohexane-l-Carbonic Acid
N-Hydroxysuccinimide Ester (SMCC)
4.1.4
(Coupling via SH Groups)
4.1.4.1
4.1.5
with
(EDAC, EDC)
4.1.6
by
4.1.7
Using Glutaraldehyde (Two-Step Procedure)
4.1.8
4.1.9
(Glutaraldehyde Protocol)
4.1.9.1
from Calf Intestine
4.1.10
4.1.11
4.1.11.1
4.1.11.2
4.2
4.3
4.4
4.4.1
4.5
4.6
4.6.1
4.6.2
4.6.3
4.7 Heidelberger
4.8
4.8.1
4.8.2
4.8.3 Immunodiffusion........................................ 152
4.8.4
4.9
4.10
4.11
4.12
4.13
4.13.1
4.13.2
4.13.3
Xiv
5
5.1
5.2
5.3
5.3.1
Isolation of Liver Cell Nuclei
5.3.2
of Surface Membranes (Sarcolemma, SL) of Heart Muscle Cells
5.3.2.1
Ouabain-Sensitive Na,K-ATPase
5.3.2.2
on Surface Membranes
5.3.2.3
and Association Kinetics of the DHP Receptors
5.3.3
Gradient Centrifugation
5.3.4
5.3.5
5.3.5.1
in CsCl Gradients
5.3.5.2
5.3.5.3
6
6.1
6.2
6.3
6.4
6.4.1
6.4.2
6.5
7
7.1
7.2
7.3 pH
7.4
7.4.1
7.4.2
and Organ
7.4.3 pH
7.4.4
8
8.1
8.2
8.3
8.4
Table of
8.5
8.6
8.7
8.8
8.9
8.10
8.11
8.12
9
9.1
9.1.1
9.1.2
9.1.3
9.2
9.2.1
9.2.2
9.2.3
9.3
9.4
9.4.1
9.4.2
9.4.3
9.4.4
Subject Index
|
| any_adam_object | 1 |
| author | Holtzhauer, Martin |
| author_facet | Holtzhauer, Martin |
| author_role | aut |
| author_sort | Holtzhauer, Martin |
| author_variant | m h mh |
| building | Verbundindex |
| bvnumber | BV021617248 |
| classification_rvk | WC 4150 |
| classification_tum | CHE 810f |
| ctrlnum | (OCoLC)633978024 (DE-599)BVBBV021617248 |
| discipline | Chemie / Pharmazie Biologie |
| edition | 1., engl. ed. |
| format | Book |
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| id | DE-604.BV021617248 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T12:37:47Z |
| institution | BVB |
| isbn | 3540327851 9783540327851 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014832367 |
| oclc_num | 633978024 |
| open_access_boolean | |
| owner | DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-19 DE-BY-UBM DE-634 DE-11 DE-578 |
| owner_facet | DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-19 DE-BY-UBM DE-634 DE-11 DE-578 |
| physical | XVIII, 251 S. graph. Darst. 235 mm x 155 mm |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Springer |
| record_format | marc |
| series2 | Springer Lab Manual |
| spellingShingle | Holtzhauer, Martin Basic methods for the biochemical lab 23 figures and 86 tables Biochemie (DE-588)4006777-4 gnd Labortechnik (DE-588)4123602-6 gnd Laboratorium (DE-588)4033927-0 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4006777-4 (DE-588)4123602-6 (DE-588)4033927-0 (DE-588)4038971-6 |
| title | Basic methods for the biochemical lab 23 figures and 86 tables |
| title_alt | Biochemische Labormethoden |
| title_auth | Basic methods for the biochemical lab 23 figures and 86 tables |
| title_exact_search | Basic methods for the biochemical lab 23 figures and 86 tables |
| title_full | Basic methods for the biochemical lab 23 figures and 86 tables Martin Holtzhauer |
| title_fullStr | Basic methods for the biochemical lab 23 figures and 86 tables Martin Holtzhauer |
| title_full_unstemmed | Basic methods for the biochemical lab 23 figures and 86 tables Martin Holtzhauer |
| title_short | Basic methods for the biochemical lab |
| title_sort | basic methods for the biochemical lab 23 figures and 86 tables |
| title_sub | 23 figures and 86 tables |
| topic | Biochemie (DE-588)4006777-4 gnd Labortechnik (DE-588)4123602-6 gnd Laboratorium (DE-588)4033927-0 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Biochemie Labortechnik Laboratorium Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014832367&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT holtzhauermartin biochemischelabormethoden AT holtzhauermartin basicmethodsforthebiochemicallab23figuresand86tables |
Inhaltsverzeichnis
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Teilbibliothek Chemie
| Signatur: |
0302 CHE 810f 2006 A 6874 Lageplan |
|---|---|
| Exemplar 1 | Ausleihbar Am Standort |
Teilbibliothek Weihenstephan
| Signatur: |
1002 CHE 810f 2006 A 7843 Lageplan |
|---|---|
| Exemplar 1 | Ausleihbar Am Standort |