Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Buch |
| Sprache: | Englisch Deutsch |
| Veröffentlicht: |
2005
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| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014724443&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | Köln, Univ., Diss., 2005 |
| Umfang: | IV, 55 Bl. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Keller, Anna Maria |d 1976- |e Verfasser |0 (DE-588)131525328 |4 aut | |
| 245 | 1 | 0 | |a Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro |c vorgelegt von Anna Maria Keller |
| 264 | 1 | |c 2005 | |
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| 500 | |a Köln, Univ., Diss., 2005 | ||
| 546 | |a Zsfassung in dt. Sprache | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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Datensatz im Suchindex
| _version_ | 1819355080610021376 |
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| adam_text | Table of contents
TABLE OF FIGURES I
INDEX OF TABLES II
LIST OF ABBREVIATIONS Ill
1. INTRODUCTION 1
1.1. Alzheimer s disease 1
1.2. Previous studies from our laboratory 1
1.3. a! nlcotinic acetylcholine receptor and p2 nicotinic acetylcholine receptor
subunits 2
1.4. i-protein 3
1.5. Okadaicacid 6
1.6. Description of the project 6
2. AIM OF THE THESIS 8
3. MATERIALS 9
3.1. Animals 9
3.2. Chemicals, antibodies, buffers and reagents 9
3.2.1. Chemicals 9
3.2.2. Fetal calf serum (FCS) Invitrogen 9
3.2.3. Antibodies 10
3.2.4. Buffers and reagents 10
3.2.5. Providers (confer 3.2.1, 3.2.2) : 11
3.3. Technical equipment 12
4. METHODS 13
4.1. Primary hippocampal cell culture 13
4.2. Preparation and incubation 13
4.3. Lactate dehydrogenase-estimation 14
4.4. Immunofluorescence 15
4.5. Immunofluorescence microscopy 16
4.6. Quantitative assessment of immunocytochemical findings 16
4.7. Statistical Analysis 18
5. RESULTS 19
5.1. Morphological appearance of cultured neurons 19
5.2. Lactate dehydrogenase-estimation 21
5.3. MAP2-immunoreactive neurons 22
5.4. o7-immunoreactive and AT8-immunoreactive neurons In double staining 25
5.5. p2-immunoreactive and AT8-immunoreactive neurons in double staining 33
6. DISCUSSION 40
6.1. Methods 40
6.2. Results 41
6.2.1. The influence of okadaic acid on AT8-immunoreactivity 41
6.2.2. The influence of 0,5uM Ap,^ on AT8-immunoreactivity 42
6.2.3. The influence of okadaic acid in the presence of Api^2 on AT8-immunoreactivity.. 43
6.2.4. The influence of okadaic acid on (52- and a7-nicotinic acetylcholine receptor-
immunoreactivity 43
6.2.5. The influence of 0,5uM Ap,^2on p2- and a7-nicotinic acetylcholine receptor-
immunoreactivity 44
6.2.6. The influence of okadaic acid in the presence or absence of A(J,.« on double
stained cells 44
6.3. Conclusion , 44
7. SUMMARY 46
8. ZUSAMMENFASSUNG 47
9. REFERENCES 48
10. PRELIMINARY PUBLICATIONS 55
I
Table of figures
Figure 1: Subunit Topology of the nicotmic acetylcholine receptor 3
Figure 2: Receptor Assembly 3
Figure 3: Human T-protein [17] 4
Figure 4: Hyperphosphorylation sites of T-protein and corresponding specific
antibo-dies 7
Figure 5: Immunfluorescence labelling for nicotinic acetylcholine receptor-
subunit P2 (A, C) and AT8 (B, D) 20
Figure 6: LDH-Estimation (cf. Table 1) 22
Figure 7: The morphology of MAP2-immunoreactive neurons
(immunofluorescence labelling) 23
Figure 8: Effects of APn2 and okadaic acid on MAP2-immunoreactive cells (cf.
to Table 2) 24
Figure 9: Morphology of MAP2-immunoreactive neurons in vehicle treated
cultures (A) and after treatment with APi^2+1nM OA (B), Ap,.,2 +5nM
OA (C) and 10nM OA only (D) 25
Figure 10: Confocal microscopy of a7-nicotinic acetylcholine receptor-
immunoreactivity (A) (Cy3-conjugated, red), hyperphosphorylated
T-protein (AT8) (C) (Cy2-conjugated, green) and overlay (B) in the
presence of 0,5 uM Ap1J2+5nM okadaic acid 27
Figure 11: Confocal microscopy of a7 (A) (Cy3-conjugated, red),
hyperphosphorylated T-protein (AT8) (C) (Cy2-conjugated, green) and
overlay (B) in the presence of 5nM okadaic acid 28
Figure 12: Effects of ApM2 and okadaic acid on the a7-immunoreactive neurons
in comparison to MAP2 (cf. Table 3) 30
Figure 13: Effects of APi^2 and okadaic acid on the AT3-immunoreactive neurons
in comparison to MAP2-immunoreactivity (cf. Table 3) 31
Figure 14: Effects of ApM2 and okadaic acid on a7+AT8 double stained neurons
in comparison to MAP2-immunoreactivity 32
Figure 15: Confocal microscopy of nicotinic acetylcholine receptor-subunit p2-IR
(A) (Cy3-conjugated, red), hyperphosphorylated T-protein (ATB)-IR
(C) (Cy2-conjugated, green) and overlay (B) in the presence of 0,5 uM
Ap,.42 and 5nM OA 34
Figure 16: Confocal microscopy of nicotinic acetylcholine receptor-subunit p2 (A)
(Cy3-conjugated, red), hyperphosphorylated T-protein (AT8) (C) (Cy2-
conjugated, green), and overlay (B) in the presence of 5nM OA 35
Figure 17: Effects of APn2 and okadaic acid on p2-immunoreactive cells in
comparison to MAP2-immunoreactivity (cf. Table 4) 37
Figure 18: Effects of Api_ 2 and OA on AT8-immunoreactive cells in comparison
to MAP2-immunoreactivity (cf. Table 4) 38
Figure 19: Effects of Apn2 and OA on P2 + AT8 double stained cells in
comparison to MAP2-immunoreactivity 39
II
Index of Tables
Table 1: Lactate dehydrogenase (LDH) activity levels upon incubation with
okadaic acid (OA), APn2 and APn2 + okadaic acid 21
Table 2: Numbers of MAP2 immunoreactive neurons upon incubation with
okadaic acid (OA), APn2 and APi^2 + okadaic acid 24
Table 3: Numbers of al nicotinic acetylcholine receptor- and AT8
immunoreactive neurons upon incubation with okadaic acid (OA), ApM2
and Ap,_,2 + okadaic acid 29
Table 4: Numbers of (32 nicotinic acetylcholine receptor- and AT8
immunoreactive neurons upon incubation with okadaic acid (OA), AP^2
and Ap,_,2 + okadaic acid 36
|
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| author | Keller, Anna Maria 1976- |
| author_GND | (DE-588)131525328 |
| author_facet | Keller, Anna Maria 1976- |
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| author_sort | Keller, Anna Maria 1976- |
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| building | Verbundindex |
| bvnumber | BV021507789 |
| ctrlnum | (OCoLC)257169488 (DE-599)BVBBV021507789 |
| discipline | Medizin |
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| id | DE-604.BV021507789 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T12:35:12Z |
| institution | BVB |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014724443 |
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| owner_facet | DE-355 DE-BY-UBR |
| physical | IV, 55 Bl. Ill., graph. Darst. |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
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| spellingShingle | Keller, Anna Maria 1976- Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro |
| subject_GND | (DE-588)4113937-9 |
| title | Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro |
| title_auth | Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro |
| title_exact_search | Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro |
| title_full | Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro vorgelegt von Anna Maria Keller |
| title_fullStr | Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro vorgelegt von Anna Maria Keller |
| title_full_unstemmed | Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro vorgelegt von Anna Maria Keller |
| title_short | Comparison of expression patterns of the alpha7- and beta2-subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau-protein under the influence of okadaic acid and Abeta1-42 in vitro |
| title_sort | comparison of expression patterns of the alpha7 and beta2 subunits of the nicotinic acetylcholine receptor and of hyperphosphorylated tau protein under the influence of okadaic acid and abeta1 42 in vitro |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014724443&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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