Receptor tyrosine kinase activation in human carcinoma cells:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2004
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010744356&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | München, Univ., Diss., 2004 |
| Umfang: | V, 98 Bl. graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV017918354 | ||
| 003 | DE-604 | ||
| 007 | t| | ||
| 008 | 040310s2004 xx d||| m||| 00||| eng d | ||
| 016 | 7 | |a 970400187 |2 DE-101 | |
| 035 | |a (OCoLC)64655678 | ||
| 035 | |a (DE-599)BVBBV017918354 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-19 |a DE-12 | ||
| 100 | 1 | |a Fischer, Oliver Martin |d 1975- |e Verfasser |0 (DE-588)128791098 |4 aut | |
| 245 | 1 | 0 | |a Receptor tyrosine kinase activation in human carcinoma cells |c von Oliver Martin Fischer |
| 264 | 1 | |c 2004 | |
| 300 | |a V, 98 Bl. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a München, Univ., Diss., 2004 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010744356&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-010744356 | |
Datensatz im Suchindex
| _version_ | 1820954473840771072 |
|---|---|
| adam_text |
CONTENTS
I
CONTENTS
1.
INTRODUCTION
1
1.1.
RECEPTOR
TYROSINE
KINASES
1
1.1.1.
THE
EGFR
FAMILY
2
1.1.2.
EGF-LIKE
LIGANDS
AND
RECEPTOR
ACTIVATION
3
1.1.3.
MET
-
THE
RECEPTOR
FOR
HEPATOCYTE
GROWTH
FACTOR
5
1.2.
RTK
DOWNSTREAM
SIGNALLING AND
PROTEIN
INTERACTION
DOMAINS
6
1.3.
G
PROTEIN-COUPLED
RECEPTORS
7
1.3.1.
HETEROTRIMERIC
G
PROTEINS
8
1.3.2.
THE
ONCOGENIC
POTENTIAL
OF
GPCRS
AND
G
PROTEINS
8
1.4.
MITOGEN-ACTIVATED
PROTEIN
KINASES
9
1.5.
RTK
TRANSACTIVATION
11
1.5.1.
EGFR
ACTIVATION
BY
CELLULAR
STRESS
11
1.5.2.
EGFR
SIGNAL
TRANSACTIVATION
12
1.5.3.
ADAM
FAMILY
METALLOPROTEASES
13
1.5.4.
RECEPTOR
CROSS-TALK
BEYOND
EGFR
SIGNAL
TRANSACTIVATION
14
1.5.5.
REACTIVE
OXYGEN
SPECIES
IN
SIGNAL
TRANSDUCTION
15
1.5.6.
GROWTH
FACTOR-STIMULATED
ROS
PRODUCTION
:
NADPH
OXIDASES
16
1.6.
AIM
OF
THE
STUDY
17
2.
MATERIALS
AND
METHODS
18
2.1.
MATERIALS
18
2.1.1.
LABORATORY
CHEMICALS
AND
BIOCHEMICALS
18
2.1.2.
ENZYMES
19
2.1.3.
RADIOCHEMICALS
19
2.1.4.
YYKITS"
AND
OTHER
MATERIALS
-
19
2.1.5.
GROWTH
FACTORS
AND
LIGANDS
20
2.1.6.
MEDIA
AND
BUFFERS
20
2.1.7.
CELL
CULTURE
MEDIA
20
2.1.8.
STOCK
SOLUTIONS
FOR
BUFFERS
20
2.1.9.
BACTERIAL
STRAINS,
CELL
LINES
AND
ANTIBODIES
22
2.1.9.1.
E.COLI
STRAINS
22
2.1.9.2.
CELL
LINES
22
2.1.9.3.
ANTIBODIES
22
CONTENTS
II
2.1.10.
PLASMIDS
AND
OLIGONUCLEOTIDES
23
2.1.10.1.
PRIMARY
VECTORS
23
2.1.10.2. CONSTRUCTS
24
2.1.10.3.
SIRNA-OLIGONUCLEOTIDES
24
2.2.
METHODS
IN
MOLECULAR
BIOLOGY
24
2.2.1.
PLASMID
PREPARATION
FOR
ANALYTICAL
PURPOSE
24
2.2.2.
PLASMID
PREPARATION
IN
PREPARATIVE
SCALE
25
2.3.
ENZYMATIC
MANIPULATION
OF
DNA
25
2.3.1.1.
DIGESTION
OF
DNA
SAMPLES
WITH
RESTRICTION
ENDONUCLEASES
25
2.3.1.2.
DEPHOSPHORYLATION
OF
DNA
5'-TERMINI
WITH
CALF
INTESTINE
ALKALINE
PHOSPHATASE
(CIAP)
25
2.3.1.3.
DNA
INSERT
LIGATION
INTO
VECTOR
DNA
25
2.3.1.4.
AGAROSE
GEL
ELECTROPHORESIS
25
2.3.1.5.
ISOLATION
OF
DNA
FRAGMENTS
USING
LOW
MELTING
TEMPERATURE
AGAROSE
GELS
26
2.3.2.
INTRODUCTION
OF
PLASMID
DNA
INTO
E.COLI
CELLS
26
2.3.2.1.
PREPARATION
OF
COMPETENT
CELLS
26
2.3.2.2.
TRANSFORMATION
OF
COMPETENT
CELLS
26
2.3.3.
OLIGONUCLEOTIDE-DIRECTED
MUTAGENESIS
26
2.3.3.1.
PREPARATION
OF
URACIL-CONTAINING,
SINGLE-STRANDED
DNA
TEMPLATE
26
2.3.3.2.
PRIMER
EXTENSION
27
2.3.4.
ENZYMATIC
AMPLIFICATION
OF
DNA
BY
POLYMERASE
CHAINJEACTION
(PCR)
27
2.3.5.
DNA
SEQUENCING
27
2.3.6.
CDNA
ARRAY
HYBRIDIZATION
28
2.3.7.
RT-PCR
ANALYSIS
28
2.4.
METHODS
IN
MAMMALIAN
CELL
CULTURE
28
2.4.1.
GENERAL
CELL
CULTURE
TECHNIQUES
28
2.4.2.
TRANSFECTION
OF
CULTURED
CELL
LINES
29
2.4.2.1.
TRANSFECTION
OF
CELLS WITH CALCIUM
PHOSPHATE
29
2.4.2.2.
TRANSFECTION
OF
COS-7
CELLS
USING
LIPOFECTAMINE
29
2.4.2.3.
RNA
INTERFERENCE
29
2.4.2.4.
APOPTOSIS
ASSAY
30
2.5.
PROTEIN
ANALYTICAL
METHODS
30
2.5.1.
LYSIS
OF
CELLS
WITH
TRITON
X-100
30
CONTENTS
III
2.5.2.
DETERMINATION
OF
PROTEIN
CONCENTRATION
IN
CELL
LYSATES
30
2.5.3.
IMMUNOPRECIPITATION
AND
IN
VITRO
ASSOCIATION
WITH
FUSION
PROTEINS
30
2.5.4.
TCA
PRECIPITATION
OF
PROTEINS
IN
CONDITIONED
MEDIUM
30
2.5.5.
SDS-POLYACRYLAMIDE-GELELECTROPHORESIS
(SDS-PAGE)
30
2.5.6.
TRANSFER
OF
PROTEINS
ON
NITROCELLULOSE
MEMBRANES
31
2.5.7.
IMMUNOBLOT
DETECTION
31
2.5.8.
DIFFERENTIAL
DETERGENT
FRACTIONATION
31
2.6.
BIOCHEMICAL
AND
CELL
BIOLOGICAL
ASSAYS
31
2.6.1.
STIMULATION
OF
CELLS
31
2.6.2.
ERK1/2
PHOSPHORYLATION
32
2.6.3.
ERK/MAPK
ACTIVITY
32
2.6.4.
JNK
ACTIVITY
ASSAY
32
2.6.5.
FLOW
CYTOMETRIC
ANALYSIS
OF
CELL
SURFACE
PROTEINS
32
2.6.6.
DETECTION
OF
ROS
BY
FLOW
CYTOMETRIC
ANALYSIS
33
2.6.7.
INCORPORATION
OF
3
H-THYMIDINE
INTO
DNA
33
2.7.
STATISTICAL
ANALYSIS
33
3.
RESULTS
34
3.1.
EGFR
SIGNAL
TRANSACTIVATION
IN
PANCREATIC
AND
NON-SMALL
CELL
LUNG
CARCINOMA
34
3.1.1.
GPCR-INDUCED
EGFR
PHOSPHORYLATION
IN
NSCLC
AND
PANCREATIC
CARCINOMA
34
3.1.2.
EGFR
SIGNAL
TRANSACTIVATION-INDUCED
DOWNSTREAM
SIGNALLING
35
3.1.3.
LPA
STIMULATION
INDUCES
DNA
SYNTHESIS
IN
NCI-H292
C&LLS
38
3.2.
STRESS
SIGNALLING
IN
HUMAN
TUMOUR
CELLS
IS
MEDIATED
BY
HB-EGF
AND
ADAM
PROTEASES
39
3.2.1.
DISTINCT
KINETICS
OF
EGFR
AND
MAPK
ACTIVATION
IN
COS-7
AND
HUMAN
CARCINOMA
CELL
LINES
39
3.2.2.
STRESS-INDUCED
EGFR
ACTIVATION
IS
INDEPENDENT
OF
SRC
ACTIVITY
41
3.2.3.
REDOX-DEPENDENT
ACTIVATION
OF
GOCI/
0
IS
NOT
INVOLVED
IN
STRESS-INDUCED
EGFR
ACTIVATION
41
3.2.4.
P38
CONTROLS
EGFR
ACTIVATION
BY
OSMOTIC
AND
OXIDATIVE
STRESS
42
3.2.5.
STRESS-INDUCED
EGFR
PHOSPHORYLATION
DEPENDS
ON
METALLOPROTEASE
ACTIVITY
AND
HB-EGF
FUNCTION
44
3.2.6.
ECTODOMAIN
SHEDDING
OF
PROHB-EGF
IS
INDUCED
IN
RESPONSE
TO
OSMOTIC
AND
OXIDATIVE
STRESS
IN
COS-7
CELLS
46
CONTENTS
IV
3.2.7.
METALLOPROTEASES
OF
THE
ADAM
FAMILY
MEDIATE
EGFR
ACTIVATION
BY
OSMOTIC
AND
OXIDATIVE
STRESS
48
3.2.8.
ACTIVATION
OF
THE
MAPKS
ERK1/2
AND
JNK
IN
RESPONSE
TO
HYPEROSMOLARITY
AND
OXIDATIVE
STRESS
IS
MEDIATED
BY
HB-EGF-
DEPENDENT
EGFR
ACTIVATION
50
3.2.9.
BLOCKADE
OF
HB-EGF
FUNCTION
STRONGLY
ENHANCES
DOXORUBICIN-INDUCED
CELL
DEATH
53
3.3.
MET
RECEPTOR
TRANSACTIVATION
BY
GPCRS
AND
EGFR
IS
MEDIATED
BY
ROS
55
3.3.1.
ANALYSIS
OF
CELL
SURFACE
PROTEIN
PHOSPHORYLATION
IN
RESPONSE
TO
GPCR
STIMULATION
IN
PANCREATIC
CARCINOMA
CELLS
55
3.3.2.
GPCR
AND
EGFR
STIMULATION
INDUCE
MET
RECEPTOR
TYROSINE
PHOSPHORYLATION
56
3.3.3.
GPCR-INDUCED
MET
TRANSACTIVATION
OCCURS
INDEPENDENT
OF
EGFR
KINASE
ACTIVITY
57
3.3.4.
LPA-INDUCED
MET
TRANSACTIVATION
IS
INDEPENDENT
OF
SRC,
PKC,
PI3K
AND
MEK1/2
ACTIVITY
58
3.3.5.
MET
RECEPTOR
TRANSACTIVATION
IS
INDEPENDENT
OF
SERINE
PROTEASES
59
3.3.6.
GPCR
AND
EGF-STIMULATED
ROS
PRODUCTION
IN
CARCINOMA
CELL
LINES
60
3.3.7.
MET
RECEPTOR
TRANSACTIVATION
INVOLVES
NADPH
OXIDASE
ACTIVITY
62
3.3.8.
MET
RECEPTOR
TRANSACTIVATION
IS
INDEPENDENT
OF
NO-SYNTHASE
ACTIVITY
64
3.3.9.
MET
TRANSACTIVATION
INDUCES
DISSOCIATION
OF
THE
B-CATENIN-MET
RECEPTOR
COMPLEX
IN
HUH7
AND
DAN-G CELLS
65
4.
DISCUSSION
67
4.1.
METALLOPROTEASE-MEDIATED
EGFR
SIGNAL
TRANSACTIVATION
IN
NSCLC
AND
PANCREATIC
CARCINOMA
CELL
LINES
67
4.2.
EGFR
ACTIVATION
IN
RESPONSE
TO
CELLULAR
STRESS
68
4.2.1.
OSMOTIC
AND
OXIDATIVE
STRESS
MEDIATE
METALLOPROTEASE
AND
HB-EGF
DEPENDENT
EGFR
ACTIVATION
69
4.2.2.
ADAM
FAMILY
PROTEASES
MEDIATE
STRESS-INDUCED
EGFR
PHOSPHORYLATION
69
4.2.3.
P38
CONTROLS
EGFR
ACTIVATION
UPON
CELLULAR
STRESS
70
4.2.4.
THE
ROLE
OF
HB-EGF
FUNCTION
IN
DOXORUBICIN-INDUCED
APOPTOSIS
OF
CANCER
CELLS
71
4.3.
MET
RECEPTOR
TRANSACTIVATION
72
4.3.1.
EGF-INDUCED
MET
RECEPTOR
PHOSHORYLATION
73
CONTENTS
V
4.3.2.
THE
ROLE
OF
ROS
PRODUCTION
IN
EGF-STIMULATED
MET
RECEPTOR
TRANSACTIVATION
73
4.3.3.
GPCR
AGONIST-INDUCED
MET
RECEPTOR
TRANSACTIVATION
74
4.3.4.
MET
RECEPTOR
TRANSACTIVATION
INDUCES
DISSOCIATION
OF
THE
P-CATENIN-MET
COMPLEX
75
4.4.
PERSPECTIVES
76
5.
SUMMARY
78
6.
REFERENCES
79
7.
ABBREVIATIONS
96 |
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| author | Fischer, Oliver Martin 1975- |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV017918354 |
| illustrated | Illustrated |
| indexdate | 2025-01-11T12:11:52Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010744356 |
| oclc_num | 64655678 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-12 |
| owner_facet | DE-19 DE-BY-UBM DE-12 |
| physical | V, 98 Bl. graph. Darst. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| record_format | marc |
| spelling | Fischer, Oliver Martin 1975- Verfasser (DE-588)128791098 aut Receptor tyrosine kinase activation in human carcinoma cells von Oliver Martin Fischer 2004 V, 98 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2004 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010744356&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fischer, Oliver Martin 1975- Receptor tyrosine kinase activation in human carcinoma cells |
| subject_GND | (DE-588)4113937-9 |
| title | Receptor tyrosine kinase activation in human carcinoma cells |
| title_auth | Receptor tyrosine kinase activation in human carcinoma cells |
| title_exact_search | Receptor tyrosine kinase activation in human carcinoma cells |
| title_full | Receptor tyrosine kinase activation in human carcinoma cells von Oliver Martin Fischer |
| title_fullStr | Receptor tyrosine kinase activation in human carcinoma cells von Oliver Martin Fischer |
| title_full_unstemmed | Receptor tyrosine kinase activation in human carcinoma cells von Oliver Martin Fischer |
| title_short | Receptor tyrosine kinase activation in human carcinoma cells |
| title_sort | receptor tyrosine kinase activation in human carcinoma cells |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010744356&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT fischerolivermartin receptortyrosinekinaseactivationinhumancarcinomacells |