Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Aachen
Shaker
2000
|
| Ausgabe: | Als Ms. gedr. |
| Schriftenreihe: | Berichte aus der Biotechnologie
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008969066&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | Zugl.: München, Techn. Univ., Diss., 2000 |
| Umfang: | 160 S. Ill., graph. Darst. : 21 cm |
| ISBN: | 3826572319 |
Internformat
MARC
| LEADER | 00000nam a22000008c 4500 | ||
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| 001 | BV013164009 | ||
| 003 | DE-604 | ||
| 005 | 20001215 | ||
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| 008 | 000516s2000 gw ad|| m||| 00||| eng d | ||
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| 049 | |a DE-12 |a DE-91 | ||
| 084 | |a PHY 823d |2 stub | ||
| 100 | 1 | |a Wiegand, Gerald |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy |c Gerald Wiegand |
| 250 | |a Als Ms. gedr. | ||
| 264 | 1 | |a Aachen |b Shaker |c 2000 | |
| 300 | |a 160 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Berichte aus der Biotechnologie | |
| 500 | |a Zugl.: München, Techn. Univ., Diss., 2000 | ||
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| 689 | 0 | 1 | |a Elektrische Eigenschaft |0 (DE-588)4193812-4 |D s |
| 689 | 0 | 2 | |a Impedanzspektroskopie |0 (DE-588)4161400-8 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008969066 | |
Datensatz im Suchindex
| DE-BY-TUM_call_number | 0001 DM 20648 |
|---|---|
| DE-BY-TUM_katkey | 1156434 |
| DE-BY-TUM_location | Mag |
| DE-BY-TUM_media_number | 040006756354 |
| _version_ | 1821932343518035968 |
| adam_text |
CONTENTS
1
SUMMARY
.
_
.
_
.
_
1
2
INTRODUCTION
AND
MOTIVATION
.
3
2.1
INTRODUCTION
.
3
2.2
MOTIVATION
.
6
3
TECHNIQUE:
IMPEDANCE
SPECTROSCOPY
.9
3.1
STANDARD
IMPEDANCE
SPECTROSCOPY
(IS)
.
11
3.1.1
IMPEDANCE
MEASUREMENTS
IN
THE
FREQUENCY
DOMAIN
.
11
3.1.2
THE
IS
SETUP
.
12
3.1.2.1
HARDWARE
.
12
3.1.2.2
MEASURING
CELL
.
13
3.1.2.3
SOFTWARE
.
14
3.2
REPRESENTATION
OF
IMPEDANCE
SPECTRA
.
14
3.3
EQUIVALENT
CIRCUITS
MODELING:
ELECTRICAL
ANALOGS
OF
PHYSICAL
AND
CHEMICAL
PROCESSES
.
18
3.4
FAST
IMPEDANCE
SPECTROSCOPY
(FIS)
.
20
3.4.1
IMPEDANCE
MEASUREMENTS
IN
THE
TIME
DOMAIN
.
20
3.4.1.1
PERTURBATION
SIGNAL
.
21
3.4.2
SIGNAL
PROCESSING
.
23
3.4.2.1
SHORT-TIME
FOURIER
TRANSFORM(STFT):
ANALYSIS
WITH
FIXED
RESOLUTION
.
24
3.4.2.2
WAVELET
TRANSFORM
(WT):
MULTIRESOLUTION
ANALYSIS
.
27
3.4.3
EQUIVALENT
CIRCUIT
MODELING
IN
FIS
.
29
3.4.4
SIMULATION
OF
SINGLE
ION
CHANNEL
MEASUREMENTS
IN
SUPPORTED
MEMBRANES
BY
FIS.
.29
3.4.4.1
BOUNDARY
CONDITIONS
.
29
3.4.4.2
SIMULATIONS
.
31
3.4.5
THE
FIS
SETUP
.
34
3.4.5.1
HARDWARE
.
34
3.4.5.2
SOFTWARE
.
36
3.4.6
PERFORMANCE
STUDY
OF
THE
FIS
.
36
3.4.6.1
STANDARD
MODEL
.
37
3.4.6.2
FAST
SWITCHING
MODEL
.
38
3.4.6.3
GIGAOHMIC
MODEL
.
40
3.4.7
DISCUSSION
OF
THE
FIS
PERFORMANCE
.
41
4
METHODS
OF
PREPARATION
.
43
4.1
SUBSTRATES
WITH
ARRAYS
OF
PLANAR
THIN-FILM
ELECTRODES
.
43
4.1.1
CLEANING
PROCEDURE
.
43
4.1.2 SEMICONDUCTOR
MICROMACHING
.
43
4.1.3
MATERIAL
AND
INTERFACIAL
PROPERTIES
.
45
4.2
ELECTROLYTIC
BUFFER
SOLUTIONS
.
46
4.2.1
STANDARD
BUFFER
.
.46
4.2.2
ELECTROLYTES
WITHOUT
SMALL
CATIONS
.
47
4.3
ULTRATHIN
HYDRATED
CELLULOSE
FILMS
.
47
4.3.1
POLYMER
DEPOSITION
.
47
4.3.2
CHEMICAL
CONVERSION
.
47
4.4
SELF-HEALING
LIPID
BILAYERS
ON
SOLID
SUPPORT
.
48
4.4.1
VESICLE
FUSION
.
48
4.4.2
SOLVENT
EXCHANGE
.
48
4.5
INCORPORATION
OF
GRAMICIDIN
ION
CHANNELS
INTO
SUPPORTED
MEMBRANES
.
49
4.5.1
INCORPORATION
FROM
VESICLES
.
49
4.5.2
INCORPORATION
FROM
THE
AQUEOUS
PHASE
.
49
5
THEORY:
PHYSICAL
MODELS
FOR
ELECTRICAL
PROCESSES
IN
BIOPHYSICAL
SYSTEMS
.51
5.1
THE
ELECTROLYTE-SEMICONDUCTOR
SYSTEM
(ES
STRUCTURE)
.
51
5.1.1
THE
ELECTROLYTE
RESISTANCE
.
52
5.1.2
THE
SEMICONDUCTING
ITO
ELECTRODES
AND
THE
ELECTROLYTE-SEMICONDUCTOR
INTERFACE.
56
5.2
SUPPORTED
MEMBRANE
SYSTEMS
(EMES
STRUCTURE)
.
64
5.2.1
SUPPORTED
LIPID
BILAYERS
.
64
5.2.2
SUPPORTED
LIPID
BILAYERS
WITH
INTEGRATED
PORES
.
72
5.2.2.1
HIGH
CONCENTRATION
OF
PORES
IN
THE
MEMBRANE
.
72
5.2.2.2
LOW
CONCENTRATION
OF
PORES
IN
THE
MEMBRANE
.
77
5.3
ULTRATHIN
POLYMER
AND
CELLULOSE
FILMS
ON
ITO
ELECTRODES
.
78
6
EXPERIMENTAL:
ELECTRICAL
PROPERTIES
OF
SUPPORTED
MEMBRANES.81
6.1
PHENOMENOLOGY
OF
SUPPORTED
LIPID
BILAYERS
.
82
6.1.1
ELECTRICAL
CHARACTERIZATION
.
82
6.1.1.1
BILAYERS
OF
PURE
LIPIDS
AND
LIPID
ALLOYS:
EFFECT
OF
CHOLESTEROL,
CHARGE
AND
PREPARATIVE
METHODS
.
82
6.1.1.2
BILAYERS
ON
ULTRATHIN
HYDRATED
CELLULOSE
FILMS
.
86
6.1.1.3
TEMPERATURE
DEPENDENCE
.
88
6.1.1.4
DEPENDENCE
ON
ION
CONCENTRATION
AND
ION
TYPE
.
89
6.1.2
OPTICAL
CHARACTERIZATION
.
91
6.1.2.1
SPREADING
BEHAVIOR
OF
LIPID
BILAYERS
ON
SOLID
SUBSTRATES
.
91
6.1.2.2
LATERAL
DIFFUSION
OF
LIPIDS
IN
THE
BILAYERS
.
94
6.1.3
MODEL
AND
DISCUSSION
.
97
6.1.3.1
MEMBRANE-SUBSTRATE
INTERACTIONS
AND
LATERAL
MOBILITY
OF
LIPIDS
IN
SUPPORTED
MEMBRANES
.
97
6.1.3.2
BOUNDARY
EFFECTS
.
107
6.1.3.3
PERMEABILITY
OF
SUPPORTED
BILAYERS
.
ILL
6.1.3.4
BILAYER
CAPACITY,
SEMICONDUCTOR
EFFECTS
AND
CABLE
THEORY
.
120
6.2
INCORPORATION
KINETICS
AND
CONDUCTANCE
OF
GRAMICIDIN
ION
CHANNELS
.
123
6.2.1
INCORPORATION
OF
GRAMICIDIN
FROM
THE
AQUEOUS
PHASE
.
124
6.2.1.1
SELECTIVE
CONDUCTIVITY
.
124
6.2.1.2
KINETICS
OF
CHANNEL
INCORPORATION
.
127
6.2.2
INCORPORATION
OF
GRAMICIDIN
BY
VESICLE
FUSION
.
128
6.2.2.1
SELECTIVE
CONDUCTIVITY
.
128
6.2.2.2
KINETICS
OF
CHANNEL
INCORPORATION
.
130
6.3
VOLTAGE
STEP
RELAXATION
OF
SUPPORTED
MEMBRANES
.
131
6.3.1
EXPERIMENTAL
DATA
EVALUATION
.
132
6.3.2
BILAYERS
WITH
GRAMICIDIN
ION
CHANNEL
IN
THE
NON-CONDUCTING
STATE
.
136
6.3.3
BILAYERS
WITH
GRAMICIDIN
ION
CHANNEL
IN
THE
CONDUCTING
STATE
.
140
APPENDIX
.
.145
A.
1
DATA
WINDOWS
.
145
A.2
BESSEL
FILTER
.
145
A.3
EVALUATION
OF
DIFFUSION
COEFFICIENTS
FROM
BLEACHING
EXPERIMENTS
.
146
A.4
ABBREVIATIONS
.
149
A.5
MATERIALS
.
150
A.6
DEVICES
.
.150
LITERATURE
.151 |
| any_adam_object | 1 |
| author | Wiegand, Gerald |
| author_facet | Wiegand, Gerald |
| author_role | aut |
| author_sort | Wiegand, Gerald |
| author_variant | g w gw |
| building | Verbundindex |
| bvnumber | BV013164009 |
| classification_tum | PHY 823d |
| ctrlnum | (OCoLC)500042097 (DE-599)BVBBV013164009 |
| discipline | Physik Biologie |
| edition | Als Ms. gedr. |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013164009 |
| illustrated | Illustrated |
| indexdate | 2025-01-11T11:47:01Z |
| institution | BVB |
| isbn | 3826572319 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008969066 |
| oclc_num | 500042097 |
| open_access_boolean | |
| owner | DE-12 DE-91 DE-BY-TUM |
| owner_facet | DE-12 DE-91 DE-BY-TUM |
| physical | 160 S. Ill., graph. Darst. : 21 cm |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Shaker |
| record_format | marc |
| series2 | Berichte aus der Biotechnologie |
| spellingShingle | Wiegand, Gerald Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy Impedanzspektroskopie (DE-588)4161400-8 gnd Elektrische Eigenschaft (DE-588)4193812-4 gnd Lipidmembran (DE-588)4167790-0 gnd |
| subject_GND | (DE-588)4161400-8 (DE-588)4193812-4 (DE-588)4167790-0 (DE-588)4113937-9 |
| title | Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy |
| title_auth | Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy |
| title_exact_search | Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy |
| title_full | Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy Gerald Wiegand |
| title_fullStr | Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy Gerald Wiegand |
| title_full_unstemmed | Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy Gerald Wiegand |
| title_short | Fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy |
| title_sort | fundamental principles of the electric properties of supported lipid membranes investigated by advanced methods of impedance spectroscopy |
| topic | Impedanzspektroskopie (DE-588)4161400-8 gnd Elektrische Eigenschaft (DE-588)4193812-4 gnd Lipidmembran (DE-588)4167790-0 gnd |
| topic_facet | Impedanzspektroskopie Elektrische Eigenschaft Lipidmembran Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008969066&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wiegandgerald fundamentalprinciplesoftheelectricpropertiesofsupportedlipidmembranesinvestigatedbyadvancedmethodsofimpedancespectroscopy |
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