Fluorescent probes in cellular and molecular biology:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Boca Raton, Fla. u.a.
CRC Press
1994
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006541073&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 295 S. graph. Darst. |
| ISBN: | 0849368928 |
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Datensatz im Suchindex
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| adam_text | Fluorescent Probes
in
Cellular and
Molecular Biology
JanJHavik, Ph D
Institute of Physiology
Czech Academy of Sciences
Prague, Czech Republic
CRC Press
Boca Raton Ann Arbor London Tokyo
Chapter 1
FLUORESCENT PROBES
I Fluorescence Techniques Have a Bright Future 1
II Fluorescent Probes and Labels 2
A Probes of Polarity 3
B Probes of Viscosity 4
C Probes of Membrane Potential 6
D Probes for Ions 7
E Fluorescent Labels and Analogs 7
F Fluorescent Substrates 8
III Measurement of Fluorescence 8
A Principles 8
B Instruments 8
IV Limitations of Fluorescent Probe Applications 9
A Sensitivity 10
B Spatial Definition 10
C Temporal and Spatial Resolution 10
V Bibliography for Further Reading on Fluorescent Probes 10
References 10
Chapter 2
FLUORESCENT DYES AND CELLS
I Fluorescent Dyes 13
A Fluorescence of Organic Molecules 13
B General Rules Relating Chemical Structure and Fluorescence 14
II Autofluorescence of Cells (Fluorescence of Cellular Components) 17
A Emission in the Ultraviolet Region 17
B Emission in the Blue-Yellow Region 17
C Emission in the Red Region 18
HI Interaction of Cells with the Dye 19
IV Binding to the Surface 19
A Lipophilic Dyes 19
B Covalent Labels of the Surface 21
C Fluorescent Ligands 23
V Penetration Inside the Cell 23
A Diffusion — Passive and Facilitated 23
B Membrane Transport 24
C Acetylation 24
D Mechanical Permeabilization of Plasma Membrane 26
E Chemical Permeabilization of the Plasma Membrane 27
F Electric Permeabilization of the Plasma Membrane 27
G Other Techniques 28
VI Fate of the Dye Inside the Cell 28
A Basic Principles 28
B Freely Moving Indicator in the Cytoplasm 28
C Binding of the Dye Inside the Cell 28
D Compartmentation 29
E Useful Sequestration 29
F How to Avoid Compartmentation 29
VII Leakage from Cells 30
VIII Quenching External (Leaked) Dye 31
References 32
Chapter 3
THEORY OF FLUORESCENCE
I Dual Nature of Light 38
A Intensity 39
B Wavelength 39
C Polarization 40
D Coherence 41
II Interaction of Light and Matter 41
A Quantum Mechanical Description of Molecules 41
B Absorption and Emission 43
C Specificity of Fluorescence of Large Organic Molecules 44
m Information Included in the Spectra 46
A Absorption Spectrum 47
B Excitation Spectrum 47
C Emission Spectrum 47
D Mirror Symmetry 48
E Stokes Shift 48
F Four Possibilities for Plotting the Spectra 48
G Influence of the Solvent on the Fluorescence 49
IV Information Included in the Intensity of Fluorescence 52
A Absorption of Light 52
B Emission of Light 53
V Information Included in the Polarization of Fluorescence 57
A Photoselection 57
B Steady-State Emission from Rigid and Ordered Molecules 57
C Steady-State Emission from Rigid and Randomly Oriented
Molecules 58
D Steady-State Emission from Freely Moving and Randomly
Oriented Molecules 59
E Depolarization by Energy Transfer 61
F Polarization Spectra 61
VI Fluorescence Lifetime 62
A Introduction 62
B Fluorescence Decay Curve 64
C Time-Resolved Fluorescence Spectra 65
D Time-Resolved Fluorescence Polarization 66
References 68
Chapter 4
INSTRUMENTATION
I Importance of Understanding the Instrumentation 70
II A General-Purpose Spectrofluorometer 70
HI Parameters Measured 71
A Intensity 72
B Quantum Yield 72
C Emission Spectrum 72
D Excitation Spectrum 72
E Excitation-Emission Matrix 73
F Excitation-Emission Scans 73
G Polarization 73
H Lifetime 74
I Time-Resolved Fluorescence 74
IV Light Sources 74
A Continuous Sources 74
B Pulsed-Light Sources 77
V Light Detectors 79
A Photomultiplier 79
B Diode-Array Detector 83
C Cameras 83
D Image Intensifies 86
E General Properties of Cameras and Image Intensifies 87
VI Measurements of Spectra 91
A Monochromator 91
B Optical Filters 94
VIII Measurement of Polarization 97
A Polarizers 97
B Alignment of Polarizers 101
IX Lifetime Measurement 101
A Pulse Methods 102
B Modulation Techniques 102
X Time-Resolved Measurement 104
A Time-Resolved Emission Spectra 104
B Time-Resolved Polarization 104
XI Corrections for the Imperfections of Instrumentation 104
A Spectral Corrections 104
XII Geometry of Measurement and Arrangement of the Sample 108
A Fiber Optics 108
B Cell Culture Plates 108
C Cuvettes 108
D Microscopy 112
E Flow Cytometry 114
F Microspectrofluorometry 115
G Quantitative Fluorescence Microscopy 116
XIII Images and Their Storage 116
A Photographic Film 116
B Analog Storage (Tape Videorecorders) 118
C Digital Storage 119
XIV Digital Image Processing 119
A Image Enhancement 120
B Image Detection and Characterization 120
C Image Restoration 120
D Image Analysis 120
XV Superresolution 120
A Diffraction of Light 120
B Scatter and Glare 122
C Depth of Focus In Microscopy 122
XVI 3D-Reconstruction 122
XVII Sensitivity Limits 123
References 124
Chapter 5
FLUORESCENT PROBES FOR POLARITY
OF THE SOLVENT
I The Effect of Solvent Polarity on Fluorescence 125
II General and Specific Solvent Effects 128
A General Solvent Effects 128
B Specific Solvent Effects 130
III Spectral Shifts 131
A The Lippert Equation 132
IV Quantum Yield 132
V Lifetime 132
VI Theory of Interaction — Continuous or Two-State Model? 133
VII Polarity Probes 133
A ANS 133
B 2,6-ANS, BIS-ANS, 2,6-TNS, 2,6-MANS 134
C NPN 134
D Dansyl 134
E Nile Red 134
F ACRYLODAN 134
G PATMAN 135
H PRODAN 135
I DANCA 135
J LAURODAN 135
VIII The Polarity of the ANS Binding Site: A Case Report 135
References 136
Chapter 6
MEMBRANE FLUIDITY
I The Fluid-Mosaic Membrane Model 139
II Membrane Fluidity and Fluorescent Probes 141
m Fluorescence Polarization Measurement 141
A Membrane Fluidity and Polarization of Fluorescence 141
B Time-Resolved Fluorescence Anisotropy 143
C Order Parameter 144
D Steady-State Anisotropy 145
E Dyes Used for Polarization Measurement 146
IV Excimer Fluorescence 147
A Intermolecular Excimers 148
B Intramolecular Excimers 149
V Fluorescence Photobleaching Recovery 149
A Principle 150
B Interpretation of Data 150
C Requirements of the Dye 151
VI Viscosity Imaging 151
References 152
Chapter 7
MEASUREMENT OF MEMBRANE POTENTIAL
I Membrane Potential 155
II Fluorescent Probes for Membrane Potential 155
A Distributive Dyes (Slow Dyes) 156
B Electrochromic Dyes (Fast Dyes) 157
HI Principles of Calibration 159
IV Choice of Dyes: Review of Dyes and Problems 160
A Distributive Dyes 160
B Electrochromic Dyes 164
V Future Development — Membrane Potential Imaging 165
VI Conclusion 166
References 166
Chapter 8
MEASUREMENT OF INTRACELLULAR
CONCENTRATIONS OF IONS
I Importance of Measurement of Intracellular Ion Concentrations 172
II The In Situ Indicators 174
A Introduction 174
B Principles of Operation 175
C The Theory of Color Transition 176
D Significance of pK 181
E Stability of pK 182
III Monitoring Ion-Induced Fluorescence Changes 184
A Double-Excitation Approach 185
B Double-Emission Approach 185
C Single-Wavelength Approach 186
IV Theory of the Calibration Curve 186
A Ostwald s Approach 186
B Hantzsch s Approach 187
C Ostwald, Hantzsch, and Reality 188
V Ratio Imaging 189
VI Transmembrane Profiles 189
VII Loading 190
A Principles of Loading of Ion-Sensitive Indicators 190
B Side Effects of Loading — Intracellular Buffering 191
C Side Effects of Loading — Sequestration 192
VIII Calibration Procedures 192
A Calibration Procedures In Vitro 193
B Calibration Procedures In Vivo (In Situ) 193
IX List of Ion-Sensitive Dyes 196
A pH-Sensitive Dyes 196
B Calcium-Sensitive Dyes 208
C Magnesium-Sensitive Dyes 215
D Sodium- and Potassium-Sensitive Dyes 219
E Chloride-Sensitive Dyes 222
F Zinc-Sensitive Dyes 226
X Distributive pH Measurement 226
A Basic Theoretical Concepts 226
B The Effect of Compartmentation 228
C Fluorescence Quenching 229
D Requirements for a pH-Distributive Fluorescent Indicator 230
E List of pH-Distributive Fluorescent Dyes 230
References 231
Chapter 9
FLUORESCENT LABELS
I Introduction 242
II Excitation Energy Transfer 243
A Basic Principles 243
B Theory of Energy Transfer 243
C Ffirster Radius R , 244
D Donor-Acceptor Pairs with a Variable Distance 245
III Fluorescence Quenching 246
A Different Types of Quenching 246
B Static Quenching 247
C Dynamic (Collisional) Quenching 248
D Combined Dynamic and Static Quenching 249
E Distinction Between Dynamic and Static Quenching 249
F Trivial Quenching 249
G List of Fluorescence Quenchers 249
H Examples of Static and Dynamic Quenching 250
IV Best Fluorescent Labels 251
A AMCA 251
B BODIPY 252
C Cascade Blue 254
D Coumarin 255
E Eosin and Erythrosin 255
F Fluorescein 255
G NBD 255
H Phycobiliproteins 255
I Pyrene and Perylene 256
J Rhodamine 256
K Texas Red 256
L Multiple Labeling 256
V Chemically Reactive Probes 256
A Introduction 256
B Modification of Thiol Groups 257
C Modification of Amine Groups 257
D Modification of Aldehydes and Ketones 258
E Modification of Carboxylic Acid Groups 258
F Crosslinking Reagents 258
G Photoaffinity Reagents 258
H Caged Compounds 259
VI Fluorescent Analogs 260
A Analogs of Phospholipids 260
B Analogs of Fatty Acids 261
C Parinaric Acid 262
D Analogs of Sterols 262
E Analogs of Nucleotides 262
F Analogs of Platelet-Activating Factors 263
VII Site-Selective Fluorescent Dyes for In Vivo Staining 263
A Introduction 263
B Nucleic Acid Stains 264
C Mitochondrial Stains 264
D Endoplasmic Reticulum 264
E Golgi Apparatus 265
F Cytoskeleton 265
G Receptor-Selective Probes 265
H Fluorescently Labeled Lectins 265
VIII Fluorogenic Substrates and Fluoresence Analogs of Enzyme Substrates 265
A p-Galactosidase 265
B Glucuronidase 266
C Glucosidase 266
D Esterases and Lipases 266
E Phospholipases 266
F Alkaline Phosphatase 267
G Peroxidase 267
H Microsomal Dealkylase Substrates 267
I HTV-Protease 267
IX Endocytotic Probes 268
A Fluorescently Labeled Dextrans 268
B Fluorescently Labeled Latex Microspheres 268
References 268
Index 281
|
| any_adam_object | 1 |
| author | Slavík, Jan 1951-1999 |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 574 - [Unassigned] |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| id | DE-604.BV009875275 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T09:43:42Z |
| institution | BVB |
| isbn | 0849368928 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006541073 |
| oclc_num | 28585198 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-703 DE-20 DE-91G DE-BY-TUM DE-19 DE-BY-UBM DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-703 DE-20 DE-91G DE-BY-TUM DE-19 DE-BY-UBM DE-11 |
| physical | 295 S. graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | CRC Press |
| record_format | marc |
| spellingShingle | Slavík, Jan 1951-1999 Fluorescent probes in cellular and molecular biology Celbiologie gtt Fluorescentie gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Fluorescent Dyes Fluorescent probes Fluorometry methods Fluoreszenzsonde (DE-588)4320674-8 gnd Methode (DE-588)4038971-6 gnd Fluoreszenz (DE-588)4154818-8 gnd Fluoreszenzspektroskopie (DE-588)4017701-4 gnd Fluoreszenzfarbstoff (DE-588)4154826-7 gnd Molekularbiologie (DE-588)4039983-7 gnd Biologie (DE-588)4006851-1 gnd Cytologie (DE-588)4070177-3 gnd |
| subject_GND | (DE-588)4320674-8 (DE-588)4038971-6 (DE-588)4154818-8 (DE-588)4017701-4 (DE-588)4154826-7 (DE-588)4039983-7 (DE-588)4006851-1 (DE-588)4070177-3 |
| title | Fluorescent probes in cellular and molecular biology |
| title_auth | Fluorescent probes in cellular and molecular biology |
| title_exact_search | Fluorescent probes in cellular and molecular biology |
| title_full | Fluorescent probes in cellular and molecular biology Jan Slavik |
| title_fullStr | Fluorescent probes in cellular and molecular biology Jan Slavik |
| title_full_unstemmed | Fluorescent probes in cellular and molecular biology Jan Slavik |
| title_short | Fluorescent probes in cellular and molecular biology |
| title_sort | fluorescent probes in cellular and molecular biology |
| topic | Celbiologie gtt Fluorescentie gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Fluorescent Dyes Fluorescent probes Fluorometry methods Fluoreszenzsonde (DE-588)4320674-8 gnd Methode (DE-588)4038971-6 gnd Fluoreszenz (DE-588)4154818-8 gnd Fluoreszenzspektroskopie (DE-588)4017701-4 gnd Fluoreszenzfarbstoff (DE-588)4154826-7 gnd Molekularbiologie (DE-588)4039983-7 gnd Biologie (DE-588)4006851-1 gnd Cytologie (DE-588)4070177-3 gnd |
| topic_facet | Celbiologie Fluorescentie Wetenschappelijke technieken Wissenschaftliches Arbeiten Fluorescent Dyes Fluorescent probes Fluorometry methods Fluoreszenzsonde Methode Fluoreszenz Fluoreszenzspektroskopie Fluoreszenzfarbstoff Molekularbiologie Biologie Cytologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006541073&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT slavikjan fluorescentprobesincellularandmolecularbiology |
Inhaltsverzeichnis
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Teilbibliothek Physik
| Signatur: |
0202 BIO 040f 2002 A 1569 Lageplan |
|---|---|
| Exemplar 1 | Ausleihbar Am Standort |
Handapparate (nicht verfügbar)
| Signatur: |
0292 C.2.550a Lageplan |
|---|---|
| Exemplar 1 | Dauerhaft ausgeliehen Ausgeliehen |