An introduction to gel chromatography:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Buch |
| Sprache: | Niederländisch |
| Veröffentlicht: |
Amsterdam
North-Holland
1971
|
| Ausgabe: | 3. print. |
| Schriftenreihe: | Laboratory techniques in biochemistry and molecular biology. Paperback edition.
1,2. |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000573428&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | S.154-396 |
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| 245 | 1 | 0 | |a An introduction to gel chromatography |
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| 490 | 1 | |a Laboratory techniques in biochemistry and molecular biology. Paperback edition. |v 1,2. | |
| 830 | 0 | |a Laboratory techniques in biochemistry and molecular biology. Paperback edition. |v 1,2. |w (DE-604)BV005871299 |9 1,2. | |
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Datensatz im Suchindex
| _version_ | 1819283689003024384 |
|---|---|
| adam_text | Contents
Chapter 1. What is gel chromatography? 157
1.1. Gel chromatography a biochemical separation method 157
1.2. The mechanism of gel chromatography 158
1.3. Characteristic properties of gel chromatography 161
Chapter 2. Basic concepts 163
2.1. Some definitions 163
2.2. Parameters used to characterize the bed 165
2.3. Parameters used to characterize the flow 167
2.4. Parameters used to characterize solute behaviour 168
2.5. Graphic representation of the relationship between molecular para¬
meters of solutes and elution properties 171
2.6. Parameters used for the characterization of gels 174
Chapter 3. Gels for chromatography 175
3.1. Definition of the term gel 175
3.2. Important properties of gels for chromatography 176
3.3. Synthesis of gels for gel chromatography 179
3.4. Dextran gels, Sephadex ® 180
3.5. Chromatographic properties of Sephadex 184
3.6. Polyacrylamide gels, Bio Gel 187
3.7. Chromatographic properties of Bio Gel 191
3.8. Agar and agarose gels 191
3.9. Chromatographic properties of agarose gels 197
3.10. Other types of gels for use in aqueous solutions 200
3.11. Gels for use in organic solvents 200
154 AN INTRODUCTION TO CiEL CHROMATOGRAPHY
Chapter 4. Equipment for gel chromatography in columns .... 205
4.1. General 205
4.2. The chromatographic column 206
4.3. Equipment for regulation of the flow 210
4.3.1. Mariotte flask 210
4.3.2. Pumps 210
4.4. Recording of elution curves 213
4.4.1. General 213
4.4.2. Automatic analysis 213
4.4.3. Monitors 213
4.4.3.1. General considerations 213
4.4.3.2. Photometric monitors 214
4.4.3.3. Other types of monitors 216
4.5. Fractions collectors 217
4.6. Thermostats 218
4.7. Connecting tubing 219
Chapter 5. Experimental technique in column gel chromatography 221
5.1. Choice of gel type 221
5.2. Particle size and particle size distribution 227
5.3. Preparation of the gels 230
5.4. Prevention of microbial growth: bacteriostatic agents 232
5.5. Drying of gels 237
5.6. Avoiding contamination, rinsing contaminated gels 238
5.7. The column dimensions, the sample size and the flow rate 239
5.7.1. The influence of column length 239
5.7.2. The volume of sample 240
5.7.3. The column diameter 241
5.7.4. The bed volume 242
5.8. Packing of the column 242
5.9. Regulation of the flow rate 248
5.10. Checking the column packing 254
5.11. Influence of sample composition 255
5.12. Sample application 258
5.13. Preparation of the bed for a new experiment 260
5.14. Increase of the effective column length 261
5.14.1. Connecting columns in series 261
5.14.2. Recycling chromotography 261
5.15. Upward flow chromatography 266
Chapter 6. Some applications of gel chromatography 269
6.1. General 269
CONTENTS 155
6.2. Analytical applications 269
6.2.1. Analytical group separation 269
6.2.2. Analytical fractionation 274
6.2.3. Measurement of protein binding and complex formation .... 279
6.2.4. Determination of molecular weights 288
6.2.5. Determination of molecular weight distributions 291
6.3. Preparative applications 295
6.3.1. Preparative group separation 295
6.3.2. Preparative fractionation 304
Chapter 7. Thin layer gel chromatography 316
7.1. General 316
7.2. Differences between ordinary thin layer chromatography and thin
layer gel chromatography 317
7.3. Equipment for thin layer gel chromatography 318
7.4. Preparation of the plate 320
7.5. Set up of apparatus and chromatographic run 322
7 6. Detection of the substances 323
7.7. Two dimensional thin layer techniques 324
7.8. Applications of thin layer gel chromatography 325
Chapter 8. Special techniques 329
8.1. Basket centrifuge separation 329
8.2. Chromatography with mixed solvents 332
8.3. Zone precipitation 332
8.4. Zone electrophoresis 335
8.5. Concentration 335
8.6. Salt gradient elution 336
Chapter 9. Some notes on the mechanism of gel chromatography 338
Acknowledgements 351
Appendix I. Manufacturers and suppliers of materials required in
gel chromatography 353
Appendix 2. Analytical procedures to be applied to effluents from
gel chromatographic columns 365
Appendix 3. Staining of thin layer gel chromatograms 374
Appendix 4. Flow dynamics in Sephadex beds 378
References 382
Subject index 391
|
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| illustrated | Not Illustrated |
| indexdate | 2024-12-20T07:28:53Z |
| institution | BVB |
| language | Dutch |
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| physical | S.154-396 |
| publishDate | 1971 |
| publishDateSearch | 1971 |
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| publisher | North-Holland |
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| series | Laboratory techniques in biochemistry and molecular biology. Paperback edition. |
| series2 | Laboratory techniques in biochemistry and molecular biology. Paperback edition. |
| spellingShingle | Fischer, L. An introduction to gel chromatography Laboratory techniques in biochemistry and molecular biology. Paperback edition. |
| title | An introduction to gel chromatography |
| title_auth | An introduction to gel chromatography |
| title_exact_search | An introduction to gel chromatography |
| title_full | An introduction to gel chromatography |
| title_fullStr | An introduction to gel chromatography |
| title_full_unstemmed | An introduction to gel chromatography |
| title_short | An introduction to gel chromatography |
| title_sort | an introduction to gel chromatography |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000573428&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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