AMPK: methods and protocols
This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulat...
Gespeichert in:
| Weitere beteiligte Personen: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | Englisch |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Ausgabe: | 1st ed. 2018 |
| Schriftenreihe: | Methods in Molecular Biology
1732 |
| Schlagwörter: | |
| Links: | https://doi.org/10.1007/978-1-4939-7598-3 https://doi.org/10.1007/978-1-4939-7598-3 https://doi.org/10.1007/978-1-4939-7598-3 |
| Zusammenfassung: | This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field |
| Umfang: | 1 Online-Ressource (XVII, 626 Seiten) Illustrationen |
| ISBN: | 9781493975983 |
| DOI: | 10.1007/978-1-4939-7598-3 |
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| illustrated | Illustrated |
| indexdate | 2024-12-20T19:23:48Z |
| institution | BVB |
| isbn | 9781493975983 |
| language | English |
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| physical | 1 Online-Ressource (XVII, 626 Seiten) Illustrationen |
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| publishDate | 2018 |
| publishDateSearch | 2018 |
| publishDateSort | 2018 |
| publisher | Springer New York |
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| series2 | Methods in Molecular Biology 1732 |
| spellingShingle | AMPK methods and protocols Proteins |
| subject_GND | (DE-588)4143413-4 |
| title | AMPK methods and protocols |
| title_auth | AMPK methods and protocols |
| title_exact_search | AMPK methods and protocols |
| title_full | AMPK methods and protocols edited by Dietbert Neumann, Benoit Viollet |
| title_fullStr | AMPK methods and protocols edited by Dietbert Neumann, Benoit Viollet |
| title_full_unstemmed | AMPK methods and protocols edited by Dietbert Neumann, Benoit Viollet |
| title_short | AMPK |
| title_sort | ampk methods and protocols |
| title_sub | methods and protocols |
| topic | Proteins |
| topic_facet | Proteins Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-7598-3 |
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