Availability: The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium

The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium:

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Bibliographic Details
Main Author:	Benninghoven-Frey, Hannah Louise 1994- (Author)
Format:	Thesis/Dissertation Book
Language:	English
Published:	Aachen [2020]
Subjects:	Hochschulschrift
Links:	https://d-nb.info/1236371984/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032866148&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Physical Description:	VIII, 67 Seiten Illustrationen, Diagramme 21 cm

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adam_text	TABLE OF CONTENTS ABSTRACT ................................................................................................................................. I ZUSAMMENFASSUNG .............................................................................................................. II TABLE OF CONTENTS ............................................................................................................... ILL INDEX OF FIGURES ................................................................................................................... V INDEX OF TABLES ................................................................................................................... VI LIST OF ABBREVIATIONS ........................................................................................................ VII 1 INTRODUCTION ................................................................................................................ 1 1.1 FUNCTION AND STRUCTURE OF THE PRIMARY CILIUM ............................................................ 1 1.2 LIFE CYCLE OF THE PRIMARY CILIUM .................................................................................. 4 1.3 PHOSPHOINOSITIDES IN EUKARYOTIC CELLS ......................................................................... 6 1.3.1 PHOSPHOINOSITIDES IN THE PRIMARY CILIUM ................................................................. 8 1.4 CILIOPATHIES ................................................................................................................. 8 1.4.1 JOUBERT SYNDROME ................................................................................................... 9 1.5 CHEMICALLY-INDUCIBLE DIMERIZATION (CID) .................................................................. 10 2 AIMS AND OBJECTIVES ................................................................................................. 12 3 MATERIAL AND METHODS .............................................................................................. 13 3.1 MATERIAL .................................................................................................................... 13 3.1.1 BUFFERS, SOLUTIONS AND REAGENTS ........................................................................... 13 3.1.2 MEDIA .................................................................................................................... 14 3.1.3 TRANSFECTION REAGENT ............................................................................................ 15 3.1.4 KITS ......................................................................................................................... 15 3.1.5 ENZYMES ................................................................................................................ 15 3.1.6 EQUIPMENT ............................................................................................................. 16 3.1.7 SOFTWARE ................................................................................................................ 17 3.1.8 BACTERIA ................................................................................................................. 18 3.1.9 PLASMID DESCRIPTION ............................................................................................... 19 3.2 METHODS .................................................................................................................... 25 3.2.1 CELL CULTURE ............................................................................................................ 25 3.2.2 FLUORESCENCE MICROSCOPY ...................................................................................... 27 3.2.3 DRUG APPLICATION .................................................................................................... 28 3.2.4 DATA ANALYSIS ......................................................................................................... 29 3.2.5 CLONING ................................................................................................................... 30 3.2.6 SEQUENCING OF PLASMIDS ......................................................................................... 33 4 RESULTS ........................................................................................................................ 34 4.1 CONTRIBUTION .............................................................................................................. 34 4.2 PH(AKT) IS RESTRICTED TO THE BASAL 75 PERCENT OF THE PRIMARY CILIUM ........................ 34 4.3 CILIARY PH(AKT) CAN BE MANIPULATED BY CHEMICALLY-INDUCIBLE DIMERIZATION ........... 36 4.4 THE PI3K INHIBITOR LY294002 REDUCES THE RATE OF PIPK-INDUCED CILIARY FISSION ....... 39 4.5 PI3K CONTRIBUTES TO THE PROCESS OF CILIARY FISSION IN NIH 3T3 CELLS ........................... 42 5 DISCUSSION .................................................................................................................. 45 5.1 DETECTION OF CILIARY PI(3,4,5)P 3 USING THE PLECKSTRIN HOMOLOGY DOMAIN OF AKT ..... 45 5.2 THE INFLUENCE OF CONSTITUTIVE EXPRESSION AND ACUTE RECRUITMENT OF PIPK ON CILIARY PI(3,4,5)P3 LEVELS AND STABILITY ............................................................................................... 48 5.3 THE INFLUENCE OF CONSTITUTIVE EXPRESSION OF INP54P ON CILIARY PH(AKT) LEVELS AND CILIARY LENGTH .......................................................................................................................... 49 5.4 THE INFLUENCE OF PI3K CLASS I RECRUITMENT ON CILIARY PI(3,4,5)P 3 LEVELS AND LENGTH.. 50 5.5 ENDOGENOUS PI3K IS INVOLVED IN THE PROCESS OF CILIARY FISSION .................................. 51 5.6 LIMITATIONS AND REMAINING QUESTIONS ...................................................................... 54 5.7 THERAPEUTIC APPROACH FOR CILIOPATHIES ..................................................................... 56 6 CONCLUSION ................................................................................................................. 57 7 REFERENCES ................................................................................................................. 58 8 ACKNOWLEDGEMENTS ................................................................................................... 63 9 DECLARATIONS ............................................................................................................... 64 10 CURRICULUM VITAE ................................................................................................... 66 IV
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spellingShingle	Benninghoven-Frey, Hannah Louise 1994- The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium
subject_GND	(DE-588)4113937-9
title	The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium
title_auth	The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium
title_exact_search	The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium
title_full	The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey
title_fullStr	The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey
title_full_unstemmed	The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium vorgelegt von Hannah Louise Benninghoven-Frey
title_short	The role of phosphoinositides and phosphoinositide 3-kinase in the primary cilium
title_sort	the role of phosphoinositides and phosphoinositide 3 kinase in the primary cilium
topic_facet	Hochschulschrift
url	https://d-nb.info/1236371984/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=032866148&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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