Availability: Role of severe obesity in healing of muscle injuries

Role of severe obesity in healing of muscle injuries:

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Bibliographic Details
Main Author:	Werner, Jens-Uwe (Author)
Format:	Thesis/Dissertation Book
Language:	English
Published:	Ulm 2018
Subjects:	Obesity. Wounds and injuries. Hochschulschrift
Links:	http://d-nb.info/1166352935/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030763358&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Physical Description:	ix, 123, XXV Blätter Illustrationen, Diagramme 30 cm

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Record in the Search Index

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adam_text	TABLE OF CONTENTS 1 2 INTRODUCTION............................................................................................................. 1.1 OBESITY YESTERDAY, TODAY AND ITS CONSEQUENCES ................................................ 1.2 FREE FATTY ACIDS, CHRONIC INFLAMMATION, AND CO-MORBIDITIES IN OBESITY ............. 1.3 STRUCTURE AND FUNCTION OF SKELETAL MUSCLE......................................................... 1.4 MACROPHAGES AND SATELLITE CELLS........................................................................ 1.5 THE PRINCIPLE BEHIND MUSCLE INJURY.................................................................. 1.6 FIBROSIS AND THE COMPLEMENT SYSTEM IN MUSCLE REGENERATION ........................... 1.7 MUSCLE REGENERATION IN DETAIL, UNDERLYING MECHANISMS AND THE ROLE OF OBESITY 1.8 AIM OF THE STUDY............................................................................................... MATERIAL AND METHODS.............................................................................................. 2.1 MATERIALS........................................................................................................... 2.1.1 CONSUMABLES.............................................................................................. 2.1.2 SOLUTIONS AND REAGENTS ............................................................................... 2.1.3 DEVICES...................................................................................................... 2.1.4 ANESTHESIA, ANIMALS, AND CHOW ................................................................... 2.1.5 K ITS............................................................................................................ 2.1.6 PRIMER FOR QPCR......................................................................................... 2.1.7 SOFTWARE..................................................................................................... . 2.1.8 MICROARRAY TOOLS......................................................................................... . 2.2 METHODS............................................................................................................ 2.2.1 ANIMAL HOUSING AND BREEDING..................................................................... 2.2.2 TRAUMATIC MUSCLE INJURY............................................................................. 2.2.3 HEMATOXYLIN AND EOSIN (HE) STAINING........................................................ 2.2.4 SIRIUS RED STAINING ..................................................................................... 2.2.5 IMMUNOHISTOCHEMICAL STAINING................................................................... 2.2.6 IN VIVO IMAGING .......................................................................................... 2.2.7 LIPID EXTRACTION AND GAS CHROMATOGRAPHY .................................. , .............. ...1 ...1 ...1 ...3 ...5 ...7 ..8 .9 14 16 16 16 17 19 20 20 21 21 22 23 23 23 26 26 26 27 28 2.2.8 RNA ISOLATION AND MICROARRAY ANALYSIS.........................................................29 2.2.9 PATHWAY ANALYSIS........................................................................................... 31 2.2.10 REAL-TIME QPCR............................................................................................. 31 2.2.11 STATISTICAL EVALUATION........................................................................................32 RESULTS.........................................................................................................................33 3.1 ESTABLISHING A MODEL FOR NORMAL WEIGHT AND OBESE C57BL/6J MICE ................... 33 3.1.1 HIGH-FAT DIET INFLUENCES OUR TRAUMA MODEL IN AN EXPECTED WAY .................... 33 3.1.2 INFLUENCE OF DIET-INDUCED OBESITY ON THE INJURY MODEL .................................. 34 3.1.3 INFLUENCE OF INJURY ON THE DIET-INDUCED OBESITY MODEL .................................. 36 3.2 INJURY TO EXTENSOR ILIOTIBIALIS ANTICUS LEADS TO TISSUE DISRUPTION WITH SUBSEQUENT REGENERATION.....................................................................................................................37 3.2.1 VALIDATING THE PROGRESSION OF INJURY BY MRI, AND HE-STAINING ..................... 37 3.3 FIBROSIS FORMATION IS ALTERED IN THE OBESE PHENOTYPE .......................................... 40 3.3.1 GENE EXPRESSION LEVELS IN FIBROSIS ARE DRAMATICALLY LOWERED IN OBESITY ........ 41 3.3.2 PATHWAY ANALYSIS OF FIBROSIS-RELATED GENES....................................................44 3.4 FATTY ACID PROFILES IN MOUSE SKELETAL MUSCLE........................................................45 3.4.1 COMPARING FATTY ACID RATIOS IN NORMAL WEIGHT AND OBESE ANIMALS .................. 46 3.4.2 PHOSPHOLIPID FRACTION ANALYSIS.......................................................................48 3.4.3 TRIGLYCERIDE FRACTION ANALYSIS........................................................................49 3.4.4 LIPID METABOLISM-RELATED GENE EXPRESSION IS SEVERELY ALTERED IN OBESITY .... 51 3.4.5 PATHWAY ANALYSIS OF LIPID METABOLISM........................................................... 54 3.5 MOLECULAR FINGERPRINT OF INJURED NORMAL WEIGHT AND OBESE C57BL/6J MICE ....... 54 3.5.1 UNBIASED MULTI-STEP PROGRAM TO ASSESS DIFFERENTIALLY EXPRESSED GENES ......... 55 3.5.2 REDUCTION IN QUANTITATIVE GENE RESPONSE TO INJURY IN OBESE MICE .................. 60 3.5.3 MINIMAL OVERLAP OF DIFFERENTIALLY EXPRESSED GENES AFTER MUSCLE INJURY ........ 60 3.5.4 SCREENING FOR HIGH IMPACT GENES IN MUSCLE REGENERATION .............................. 61 3.5.5 PATHWAY ANALYSIS OF HIGH IMPACT GENES IN MUSCLE REGENERATION .................... 64 3.6 OBESITY AND ITS INFLUENCE ON MUSCLE REGENERATION 67 3.6.1 CELLULAR KEY PLAYERS REQUIRED FOR MUSCLE REGENERATION, 3.6.2 COMPLEMENT SYSTEM IN MUSCLE REGENERATION ................................................ 71 3.6.3 VALIDATION OF GENES IN HISTOLOGY AND QPCR .................................................. 72 3.6.4 ENERGY HOMEOSTASIS IS INFLUENCED BY BLUNT MUSCLE INJURY ........................... 75 3.6.5 CELL MAINTENANCE IS REQUIRED FOR PROPER REGENERATION .................................... 76 4 DISCUSSION.................................................................................................................... 82 4.1 BLUNT MUSCLE INJURY IN NORMAL WEIGHT AND OBESE C57BL/6J MICE ........................ 82 4.2 MUSCLE TRAUMA AND ITS IMPLICATIONS ON MORPHOLOGY ............................................. 83 4.3 FIBROUS TISSUE IS AFFECTED BY OBESITY .................................................................... 84 4.4 OBESITY INFLUENCES FATTY ACID CONTENT IN SKELETAL MUSCLE ...................................... 88 4.5 THE MOLECULAR FINGERPRINT IN RESPONSE TO INJURY SUBSTANTIALLY DIFFERS IN OBESITY ..91 5 SUMMARY.................................................................................................................... 101 6 REFERENCES................................................................................................................. 102 SUPPLEMENTARY INFORMATION................................................................................................ I STATUTORY DECLARATION
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spellingShingle	Werner, Jens-Uwe Role of severe obesity in healing of muscle injuries
subject_GND	(DE-588)4113937-9
title	Role of severe obesity in healing of muscle injuries
title_auth	Role of severe obesity in healing of muscle injuries
title_exact_search	Role of severe obesity in healing of muscle injuries
title_full	Role of severe obesity in healing of muscle injuries submitted by Jens-Uwe Werner
title_fullStr	Role of severe obesity in healing of muscle injuries submitted by Jens-Uwe Werner
title_full_unstemmed	Role of severe obesity in healing of muscle injuries submitted by Jens-Uwe Werner
title_short	Role of severe obesity in healing of muscle injuries
title_sort	role of severe obesity in healing of muscle injuries
topic_facet	Hochschulschrift
url	http://d-nb.info/1166352935/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030763358&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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