Molecular Biology Techniques: A Classroom Laboratory Manual
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hand...
Gespeichert in:
Beteilige Person: | |
---|---|
Format: | Elektronisch E-Book |
Sprache: | Englisch |
Veröffentlicht: |
St. Louis
Elsevier Science
2011
|
Ausgabe: | 3rd ed |
Schlagwörter: | |
Zusammenfassung: | This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions |
Beschreibung: | Description based on publisher supplied metadata and other sources |
Umfang: | 1 online resource (227 pages) |
ISBN: | 9780123855459 9780123855442 |
Internformat
MARC
LEADER | 00000nam a2200000zc 4500 | ||
---|---|---|---|
001 | BV044184715 | ||
003 | DE-604 | ||
005 | 20180724 | ||
007 | cr|uuu---uuuuu | ||
008 | 170220s2011 xx o|||| 00||| eng d | ||
020 | |a 9780123855459 |9 978-0-12-385545-9 | ||
020 | |a 9780123855442 |c Print |9 978-0-12-385544-2 | ||
035 | |a (ZDB-30-PAD)EBC802466 | ||
035 | |a (ZDB-89-EBL)EBL802466 | ||
035 | |a (ZDB-38-EBR)ebr10509200 | ||
035 | |a (OCoLC)769342081 | ||
035 | |a (DE-599)BVBBV044184715 | ||
040 | |a DE-604 |b ger |e rda | ||
041 | 0 | |a eng | |
082 | 0 | |a 572.8 | |
100 | 1 | |a Miller, Heather B. |e Verfasser |0 (DE-588)1018607323 |4 aut | |
245 | 1 | 0 | |a Molecular Biology Techniques |b A Classroom Laboratory Manual |
250 | |a 3rd ed | ||
264 | 1 | |a St. Louis |b Elsevier Science |c 2011 | |
264 | 4 | |c © 2012 | |
300 | |a 1 online resource (227 pages) | ||
336 | |b txt |2 rdacontent | ||
337 | |b c |2 rdamedia | ||
338 | |b cr |2 rdacarrier | ||
500 | |a Description based on publisher supplied metadata and other sources | ||
520 | |a This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions | ||
650 | 4 | |a Molecular biology | |
650 | 4 | |a Molecular biology -- Laboratory manuals | |
650 | 0 | 7 | |a Molekularbiologie |0 (DE-588)4039983-7 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
689 | 0 | 0 | |a Molekularbiologie |0 (DE-588)4039983-7 |D s |
689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
689 | 0 | |8 1\p |5 DE-604 | |
700 | 1 | |a Witherow, D. Scott |e Sonstige |0 (DE-588)1018608419 |4 oth | |
700 | 1 | |a Carson, Sue |e Sonstige |0 (DE-588)1018606211 |4 oth | |
776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |a Miller, Heather |t Molecular Biology Techniques : A Classroom Laboratory Manual |
912 | |a ZDB-30-PAD | ||
883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-029591506 |
Datensatz im Suchindex
_version_ | 1818983008491798528 |
---|---|
any_adam_object | |
author | Miller, Heather B. |
author_GND | (DE-588)1018607323 (DE-588)1018608419 (DE-588)1018606211 |
author_facet | Miller, Heather B. |
author_role | aut |
author_sort | Miller, Heather B. |
author_variant | h b m hb hbm |
building | Verbundindex |
bvnumber | BV044184715 |
collection | ZDB-30-PAD |
ctrlnum | (ZDB-30-PAD)EBC802466 (ZDB-89-EBL)EBL802466 (ZDB-38-EBR)ebr10509200 (OCoLC)769342081 (DE-599)BVBBV044184715 |
dewey-full | 572.8 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 572 - Biochemistry |
dewey-raw | 572.8 |
dewey-search | 572.8 |
dewey-sort | 3572.8 |
dewey-tens | 570 - Biology |
discipline | Biologie |
edition | 3rd ed |
format | Electronic eBook |
fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03166nam a2200493zc 4500</leader><controlfield tag="001">BV044184715</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180724 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">170220s2011 xx o|||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123855459</subfield><subfield code="9">978-0-12-385545-9</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123855442</subfield><subfield code="c">Print</subfield><subfield code="9">978-0-12-385544-2</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-30-PAD)EBC802466</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-89-EBL)EBL802466</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-38-EBR)ebr10509200</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)769342081</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044184715</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.8</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Miller, Heather B.</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1018607323</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Molecular Biology Techniques</subfield><subfield code="b">A Classroom Laboratory Manual</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">3rd ed</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">St. Louis</subfield><subfield code="b">Elsevier Science</subfield><subfield code="c">2011</subfield></datafield><datafield tag="264" ind1=" " ind2="4"><subfield code="c">© 2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 online resource (227 pages)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Description based on publisher supplied metadata and other sources</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Molecular biology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Molecular biology -- Laboratory manuals</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Molekularbiologie</subfield><subfield code="0">(DE-588)4039983-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Molekularbiologie</subfield><subfield code="0">(DE-588)4039983-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Witherow, D. Scott</subfield><subfield code="e">Sonstige</subfield><subfield code="0">(DE-588)1018608419</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Carson, Sue</subfield><subfield code="e">Sonstige</subfield><subfield code="0">(DE-588)1018606211</subfield><subfield code="4">oth</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="a">Miller, Heather</subfield><subfield code="t">Molecular Biology Techniques : A Classroom Laboratory Manual</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-30-PAD</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-029591506</subfield></datafield></record></collection> |
id | DE-604.BV044184715 |
illustrated | Not Illustrated |
indexdate | 2024-12-20T17:56:17Z |
institution | BVB |
isbn | 9780123855459 9780123855442 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-029591506 |
oclc_num | 769342081 |
open_access_boolean | |
physical | 1 online resource (227 pages) |
psigel | ZDB-30-PAD |
publishDate | 2011 |
publishDateSearch | 2011 |
publishDateSort | 2011 |
publisher | Elsevier Science |
record_format | marc |
spelling | Miller, Heather B. Verfasser (DE-588)1018607323 aut Molecular Biology Techniques A Classroom Laboratory Manual 3rd ed St. Louis Elsevier Science 2011 © 2012 1 online resource (227 pages) txt rdacontent c rdamedia cr rdacarrier Description based on publisher supplied metadata and other sources This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions Molecular biology Molecular biology -- Laboratory manuals Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s 1\p DE-604 Witherow, D. Scott Sonstige (DE-588)1018608419 oth Carson, Sue Sonstige (DE-588)1018606211 oth Erscheint auch als Druck-Ausgabe Miller, Heather Molecular Biology Techniques : A Classroom Laboratory Manual 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Miller, Heather B. Molecular Biology Techniques A Classroom Laboratory Manual Molecular biology Molecular biology -- Laboratory manuals Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd |
subject_GND | (DE-588)4039983-7 (DE-588)4038971-6 |
title | Molecular Biology Techniques A Classroom Laboratory Manual |
title_auth | Molecular Biology Techniques A Classroom Laboratory Manual |
title_exact_search | Molecular Biology Techniques A Classroom Laboratory Manual |
title_full | Molecular Biology Techniques A Classroom Laboratory Manual |
title_fullStr | Molecular Biology Techniques A Classroom Laboratory Manual |
title_full_unstemmed | Molecular Biology Techniques A Classroom Laboratory Manual |
title_short | Molecular Biology Techniques |
title_sort | molecular biology techniques a classroom laboratory manual |
title_sub | A Classroom Laboratory Manual |
topic | Molecular biology Molecular biology -- Laboratory manuals Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd |
topic_facet | Molecular biology Molecular biology -- Laboratory manuals Molekularbiologie Methode |
work_keys_str_mv | AT millerheatherb molecularbiologytechniquesaclassroomlaboratorymanual AT witherowdscott molecularbiologytechniquesaclassroomlaboratorymanual AT carsonsue molecularbiologytechniquesaclassroomlaboratorymanual |