Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1): = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1)
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis/Dissertation Book |
| Language: | German |
| Published: |
Erlangen ; Nürnberg
2017
|
| Subjects: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029437482&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Physical Description: | 120 Seiten Illustrationen, Diagramme |
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| 246 | 1 | 1 | |a Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) |
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Record in the Search Index
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| adam_text |
EFFECTS OF THE VIRAL TAX PROTEIN ON
CELL-TO-CELL TRANSMISSION OF
HUMAN T-CELL LEUKEMIA VIRUS TYPE 1
(HTLV-1)
EFFEKTE DES VIRALEN TAX-PROTEINS AUF DIE
ZELL-ZELL-TRANSMISSION DES
HUMANEN T-ZELL-LEUKAEMIE-VIRUS TYP 1
(HTLV-1)
DER NATURWISSENSCHAFTLICHEN FAKULTAET
DER FRIEDRICH-ALEXANDER-UNIVERSITAET ERLANGEN-NUERNBERG
ZUR ERLANGUNG DES
DOKTORGRADES DR. RER. NAT.
VORGELEGT VON
CHRISTINE GROSS
AUS COBURG
EFFECTS OF THE VIRAL TAX PROTEIN ON CELL-TO-CELL TRANSMISSION OF
HUMAN T-CELL LEUKEMIA VIRUS TYPE 1 (HTLV-1)
I.
ZUSAMMENFASSUNG.
6
I.
SUMMARY.7
II.
INTRODUCTION.8
1. HUMAN T-CELL LEUKEMIA VIRUS TYPE 1
(HTLV-1).8
2. TRANSMISSION OF
HTLV-1.
11
3. THE TRANSACTIVATOR AND ONCOPROTEIN TAX OF
HTLV-1.13
4. THE ACTIN-BUNDLING PROTEIN
FASCIN.
14
5. THE STRUCTURAL PROTEINS COLLAGEN4ALPHAL (COL4A1) AND COLLAGEN4ALPHA2
(COL4A2). 16
III. PROJECT
RATIONALE.19
IV. MATERIALS AND
METHODS.
20
1. CELL
CULTURE.
20
2. NUCLEIC ACID METHODS AND
TRANSFECTIONS.22
3. PROTEIN
METHODS.
29
4. FUNCTIONAL
ASSAYS.
36
5.
STATISTICS.
38
V. RESULTS. 39
1. FASCIN ENHANCES HTLV-1 RELEASE AND CELL-TO-CELL TRANSMISSION
.
39
1.1 REPORTER SYSTEMS ALLOWS MONITORING OF HTLV-1
TRANSMISSION.39
1.2 REPRESSION OF ENDOGENOUS FASCIN BY RNA INTERFERENCE IMPAIRS VIRUS
RELEASE AND
HTLV-1 REPORTER ACTIVITY INDEPENDENTLY OF THE ENVELOPE TYPE
.
42
1.3 INHIBITION OF FASCIN*S FUNCTION USING SPECIFIC NANOBODIES (NBS)
RESULTS IN A
SIGNIFICANT REDUCTION OF VIRUS
TRANSMISSION.47
1.4 FUNCTIONAL KNOCKDOWN OF TAX-INDUCED FASCIN LEADS TO REDUCTION OF
BOTH VIRUS
RELEASE AND CELL-TO-CELL
TRANSMISSION.
50
1.5 FASCIN KNOCKDOWN IN HTLV-1-INFECTED T-CELLS IMPAIRS GAG PL9 RELEASE
AND
INFECTION OF TARGET
CELLS.
54
1.6 FASCIN STRONGLY ACCUMULATES WITH ACTIN AT CELL-CELL
CONTACTS.56
1.7 KNOCKDOWN OF FASCIN DOES NOT AFFECT CELL-CELL AGGREGATION BUT
STRONGLY DECREASES
CELL
ADHESION.
60
1.8 FASCIN AND THE VIRAL PROTEIN GAG LOCALIZE AT TIGHT CELL-CELL
CONTACTS AND IN CELLULAR
PROTRUSIONS.
64
1.9 EGFP-FASCIN IS PACKAGED INTO VIRUS-LIKE PARTICLES
(VLPS).66
2. COL4A1 AND COL4A2 ARE NOVEL TARGETS OF
FITLY-1/TAX.67
2.1 MICROARRAY ANALYSIS AND RT-PCR VALIDATION IDENTIFIES COL4A1 AND
COL4A2 AS
NEW TAX
TARGETS.
67
2.2 COL4A1 AND COL4A2 TRANSCRIPTS AND COL4 PROTEIN ARE SPECIFICALLY
UPREGULATED
IN HTLV-1-INFECTED
T-CELLS.73
2.3 REPRESSION OF TAX LEADS TO A DECLINE IN COL4A1 AND COL4A2
TRANSCRIPTS AND
COL4 PROTEIN IN TAX-TRANSFORMED T-CELLS
.
77
2.4 COL4A1 AND COL4A2 TRANSCRIPT, BUT NOT COL4 PROTEIN EXPRESSION IS
INDUCED BY
TAX IN TRANSFECTED
T-CELLS.78
2.5 EXPRESSION OF COL4 PROTEIN IS DIFFERENTLY AFFECTED BY SHRNAS
TARGETING COL4A1
AND
COL4A2.
80
VI.
DISCUSSION.
82
VII.
REFERENCES.
93
VIII. PUBLICATIONS AND
PRESENTATIONS.113
IX.
APPENDIX.115 |
| any_adam_object | 1 |
| author | Groß, Christine |
| author_GND | (DE-588)1124801375 |
| author_facet | Groß, Christine |
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| indexdate | 2025-01-11T19:18:14Z |
| institution | BVB |
| language | German |
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| physical | 120 Seiten Illustrationen, Diagramme |
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| spelling | Groß, Christine Verfasser (DE-588)1124801375 aut Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) vorgelegt von Christine Groß aus Coburg Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) Erlangen ; Nürnberg 2017 120 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2017 Krankheitsübertragung (DE-588)4384937-4 gnd rswk-swf Onkoproteine (DE-588)4318742-0 gnd rswk-swf HTLV-I (DE-588)4333666-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content HTLV-I (DE-588)4333666-8 s Krankheitsübertragung (DE-588)4384937-4 s Onkoproteine (DE-588)4318742-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029437482&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Groß, Christine Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) Krankheitsübertragung (DE-588)4384937-4 gnd Onkoproteine (DE-588)4318742-0 gnd HTLV-I (DE-588)4333666-8 gnd |
| subject_GND | (DE-588)4384937-4 (DE-588)4318742-0 (DE-588)4333666-8 (DE-588)4113937-9 |
| title | Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) |
| title_alt | Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) |
| title_auth | Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) |
| title_exact_search | Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) |
| title_full | Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) vorgelegt von Christine Groß aus Coburg |
| title_fullStr | Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) vorgelegt von Christine Groß aus Coburg |
| title_full_unstemmed | Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) vorgelegt von Christine Groß aus Coburg |
| title_short | Effects of the viral Tax protein on cell-to-cell transmission of Human T-cell leukemia virus type 1 (HTLV-1) |
| title_sort | effects of the viral tax protein on cell to cell transmission of human t cell leukemia virus type 1 htlv 1 effekte des viralen tax proteins auf die zell zell transmission des humanen t zell leukamie virus typ 1 htlv 1 |
| title_sub | = Effekte des viralen Tax-Proteins auf die Zell-Zell-Transmission des Humanen T-Zell-Leukämie-Virus Typ 1 (HTLV-1) |
| topic | Krankheitsübertragung (DE-588)4384937-4 gnd Onkoproteine (DE-588)4318742-0 gnd HTLV-I (DE-588)4333666-8 gnd |
| topic_facet | Krankheitsübertragung Onkoproteine HTLV-I Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029437482&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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