Availability: Development of a modular high throughput screening platform for microbial exopolysaccharide producers

Development of a modular high throughput screening platform for microbial exopolysaccharide producers:

Saved in:

Bibliographic Details
Main Author:	Rühmann, Broder 1977- (Author)
Format:	Thesis/Dissertation Book
Language:	English
Published:	Straubing Verlag Attenkofer 2016
Series:	Nachwachsende Rohstoffe in Forschung und Praxis 22
Subjects:	Polysaccharide High throughput screening Bakterien Hochschulschrift
Links:	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028932644&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Physical Description:	141 Seiten Diagramme
ISBN:	9783942742689

Staff View

MARC


LEADER	00000nam a2200000 cb4500
001	BV043516604
003	DE-604
005	20170125
007	t\|
008	160419s2016 xx \|\|\|\| m\|\|\| 00\|\|\| eng d
020			\|a 9783942742689 \|9 978-3-942742-68-9
035			\|a (OCoLC)951537544
035			\|a (DE-599)BVBBV043516604
040			\|a DE-604 \|b ger \|e rda
041	0		\|a eng
049			\|a DE-91S \|a DE-91 \|a DE-M49 \|a DE-12 \|a DE-859
084			\|a AR 26200 \|0 (DE-625)8598: \|2 rvk
084			\|a CIT 680d \|2 stub
084			\|a CIT 930d \|2 stub
084			\|a CIT 975d \|2 stub
084			\|a CIT 960d \|2 stub
100	1		\|a Rühmann, Broder \|d 1977- \|e Verfasser \|0 (DE-588)1098211340 \|4 aut
245	1	0	\|a Development of a modular high throughput screening platform for microbial exopolysaccharide producers \|c Broder Rühmann
264		1	\|a Straubing \|b Verlag Attenkofer \|c 2016
300			\|a 141 Seiten \|b Diagramme
336			\|b txt \|2 rdacontent
337			\|b n \|2 rdamedia
338			\|b nc \|2 rdacarrier
490	1		\|a Nachwachsende Rohstoffe in Forschung und Praxis \|v 22
502			\|b Dissertation \|c Technische Universität München \|d 2015
650	0	7	\|a Polysaccharide \|0 (DE-588)4131871-7 \|2 gnd \|9 rswk-swf
650	0	7	\|a High throughput screening \|0 (DE-588)4596131-1 \|2 gnd \|9 rswk-swf
650	0	7	\|a Bakterien \|0 (DE-588)4004296-0 \|2 gnd \|9 rswk-swf
655		7	\|0 (DE-588)4113937-9 \|a Hochschulschrift \|2 gnd-content
689	0	0	\|a Bakterien \|0 (DE-588)4004296-0 \|D s
689	0	1	\|a Polysaccharide \|0 (DE-588)4131871-7 \|D s
689	0	2	\|a High throughput screening \|0 (DE-588)4596131-1 \|D s
689	0		\|5 DE-604
830		0	\|a Nachwachsende Rohstoffe in Forschung und Praxis \|v 22 \|w (DE-604)BV035709535 \|9 22
856	4	2	\|m DNB Datenaustausch \|q application/pdf \|u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028932644&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA \|3 Inhaltsverzeichnis
943	1		\|a oai:aleph.bib-bvb.de:BVB01-028932644

Record in the Search Index

DE-BY-TUM_call_number	0001 DM 34061 1002 CIT 960d 2016 A 2098 1302 ERG 700f 2009 A 6650-22 1304 ERG 700f 2009 A 6650-22
DE-BY-TUM_katkey	2191014
DE-BY-TUM_location	Mag 10 13
DE-BY-TUM_media_number	040009254875 040008051118 040008050786 040008050800
_version_	1821933045452636160
adam_text	TABLE OF CONTENTS 1 IN TROD U CTION .........................................................................................................................................15 1.1 CLASSIFICATION OF MICROBIAL POLYSACCHARIDES.............................................................................. 15 1.1.1 EXTRACELLULAR POLYSACCHARIDES (E^ ................................................................................. 15 1.1.2 CAPSULAR POLYSACCHARIDES (CPS)........................................................................................16 1.2 CLASSIFICATION OF MICROBIAL POLYSACCHARIDES BASED ON MONOSACCHARIDE COMPOSITION AND CHEMICAL STRUCTURE................................................................................................................ 17 1.2.1 MONOSACCHARIDES * A HIGHLY DIVERSE CLASS OF POLYSACCHARIDE BUILDING BLOCKS ...... 17 1.2.2 STRUCTURE OF BACTERIAL EXOPOLYSACCHARIDES........................................................................18 1.3 PHYSIOLOGICAL ROLE OF EXTRACELLULAR POLYSACCHARIDES ............................................................... 20 1.4 BIOSYNTHESIS OF EXTRACELLULAR POLYSACCHARIDES N BACTERIA......................................................21 1.5 PRODUCTION OF BACTERIAL EXOPOLYSACCHARIDES............................................................................23 1.6 APPLICATIONS OF BACTERIAL EXOPOLYSACCHARIDES..........................................................................25 1.7 METHODS TO IDENTIFY THE UNEXPLORED DIVERSITY OF MICROBIAL EXOPOLYSACCHARIDES. ........ * ..28 1.8 SUGAR LABELING WITH PMP DERIVATIZATION................................................................................. 38 1.9 SCOPE OF WORK................................................................................................................................. 39 2 M ATERIAL AND M ETHOD S......................................................................................................................41 2.1 MATERIAL ......................................................................................................................................41 2.1.1 EQUIPMENT............................................................................................................................41 2.1.2 SOFTWARE AND DATABASES...................................................................................................... 42 2.1.3 SPECIAL CONSUMABLES.......................................................................................................... 43 2.1.4 ENZYMES AND REAGENTS....................................................................................................... 43 2.1.5 SUGAR STANDARDS...................................................................................................................44 2.1.6 POLYMER STANDARDS...............................................................................................................46 2.1.7 PRIMER FOR 16S AND 5.8S RRNA GENE AMPLIFICATION.........................................................46 2.2 MEDIA AND BUFFER............................................................................................................................46 2.2.1 SCREENING M EDIA.................................................................................................................47 2.2.2 TRACE ELEMENT SOLUTION....................................................................................................... 47 2.2.3 AMMONIUM-ACETATE BUFFER PH 5.6.................................................................................... 47 2.2.4 E LUENTA ...............................................................................................................................47 2.2.5 500 * POTASSIUM PHOSPHATE BUFFER (PH 5.7)................................................................47 2.3 MICROBIOLOGICAL AND MOLECULAR BIOLOGICAL METHODS..................................................................47 2.3.1 BACTERIAL STRAINS AND STRAIN STORAGE................................................................................... 47 2.3.2 STRAIN CULTIVATION.................................................................................................................47 2.3.3 PHYLOGENETIC ANALYSIS........................................................................................................ 48 2.3.3.1 16S AND 5.8S RRNA GENE AMPLIFICATION...........................................................48 2.3.3.2 AGAROSE GEL ELECTROPHORESIS..............................................................................48 2.4 GENERAL SCREENING PROTOCOL..........................................................................................................48 2.4.1 GENERAL WORKFLOW OF THE SCREENING PLATFORM................................................................... 48 2.4.2 GENERAL WORKFLOW OF THE AUTOMATED MODULAR HIGH THROUGHPUT SCREENING PLATFORM .... 49 2.5 SCREENING MODULES..........................................................................................................................49 2.5.1 VISUAL OBSERVATION OF VISCOSITY ......................................................................................... 49 2.5.2 PRECIPITATION........................................................................................................................ 49 2.5.3 GLUCOSE-ASSAY..................................................................................................................... 49 2.5.4 PYRUVATE-ASSAY.................................................................................................................... 50 2.5.5 PHENOL-SULFORIC-ACID METHOD..............................................................................................50 2.6 HT_PMP_METHOD............................................................................................................................50 2.6.1 --DERIVATIZATION..................................................................................................... 50 2.6.2 HPLC ANALYSIS OF M _ SA C C H A RID E -P M P -D 50 2.6.3 ESI-ION-TRAP PARAMETER.......................................................................................................51 2.7 LIST OF PUTATIVE EPS PRODUCERS................................................................................................... 51 3 R ESULTS..................................................................................................................................................... 53 3.1 FAST CARBOHYDRATE ANALYSIS VIA LIQUID CHROMATOGRAPHY COUPLED WITH ULTRA VIOLET AND ELECTROSPRAY IONIZATION ION TRAP DETECTION IN 96-WELL FORMAT ...................................... 53 3.2 HIGH THROUGHPUT EXOPOLYSACCHARIDE SCREENING PLATFORM: FROM STRAIN CULTIVATION TO MONOSACCHARIDE COMPOSITION AND CARBOHYDRATE FINGERPRINTING IN ONE DAY**********62 3.3 AUTOMATED MODULAR HIGH THROUGHPUT EXOPOLYSACCHARIDE SCREENING PLATFORM COUPLED WITH HIGHLY SENSITIVE CARBOHYDRATE FINGERPRINT ANALYSIS....................................75 4 D ISCU SSION ............................................................................................................................................ 101 4.1 VALIDATION OF THE SCREENING PLATFORM BY THE USE OF DIFFERENT BACTERIAL STRAINS *******101 4.2 DETAILED DISCUSSION OF THE CARBOHYDRATE FINGERPRINTS OF EXEMPLARY STRAINS **************104 4.2.1 PROPOSED IDENTIFICATION OF A POLYMER PRODUCED FROM A GLUCONACETOBACTER SP. STRAIN...........................................................................................................................104 4.2.2 PROPOSED IDENTIFICATION OF THE POLYMERS PRODUCED FROM THREE RHIZOBIA STRAINS ..... 105 4.2.3 PROPOSED IDENTIFICATION OF DIFFERENT CAPSULAR POLYSACCHARIDES FROM KLEBSIELLA .... 107 4.3 PERSPECTIVES....................................................................................................................................109 4.3.1 UNKNOWN SUGAR IDENTIFICATION, FOR EXAMPLE FOR DIFFERENT DEOXY GLUCOSES.......... 109 4.3.2 LINKAGE ANALYSIS AND SEQUENCING OF POLYSACCHARIDES....................................................110 4.3.3 FURTHER IMPROVEMENT OF THE SCREENING PLATFORM........................................................ 111 5 R EFEREN CES........................................................................................................................................... 113 6 A P P EN D IX ............................................................................................................................................. 119 7 A BBREVIATIONS..................................................................................................................................... 133 8 LIST O F FIG U RES...............................................................................137 9 LIST O F TAB LES.................................................................................................................... 139 C URRICULUM V ITA E ...................................................................................................................................141
any_adam_object	1
author	Rühmann, Broder 1977-
author_GND	(DE-588)1098211340
author_facet	Rühmann, Broder 1977-
author_role	aut
author_sort	Rühmann, Broder 1977-
author_variant	b r br
building	Verbundindex
bvnumber	BV043516604
classification_rvk	AR 26200
classification_tum	CIT 680d CIT 930d CIT 975d CIT 960d
ctrlnum	(OCoLC)951537544 (DE-599)BVBBV043516604
discipline	Chemie / Pharmazie Allgemeines Biologie
format	Thesis Book
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01935nam a2200457 cb4500</leader><controlfield tag="001">BV043516604</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20170125 </controlfield><controlfield tag="007">t\|</controlfield><controlfield tag="008">160419s2016 xx \|\|\|\| m\|\|\| 00\|\|\| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783942742689</subfield><subfield code="9">978-3-942742-68-9</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)951537544</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV043516604</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91S</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-M49</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-859</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">AR 26200</subfield><subfield code="0">(DE-625)8598:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CIT 680d</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CIT 930d</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CIT 975d</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CIT 960d</subfield><subfield code="2">stub</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Rühmann, Broder</subfield><subfield code="d">1977-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1098211340</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Development of a modular high throughput screening platform for microbial exopolysaccharide producers</subfield><subfield code="c">Broder Rühmann</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Straubing</subfield><subfield code="b">Verlag Attenkofer</subfield><subfield code="c">2016</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">141 Seiten</subfield><subfield code="b">Diagramme</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Nachwachsende Rohstoffe in Forschung und Praxis</subfield><subfield code="v">22</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Technische Universität München</subfield><subfield code="d">2015</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Polysaccharide</subfield><subfield code="0">(DE-588)4131871-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">High throughput screening</subfield><subfield code="0">(DE-588)4596131-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Bakterien</subfield><subfield code="0">(DE-588)4004296-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Bakterien</subfield><subfield code="0">(DE-588)4004296-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Polysaccharide</subfield><subfield code="0">(DE-588)4131871-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">High throughput screening</subfield><subfield code="0">(DE-588)4596131-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Nachwachsende Rohstoffe in Forschung und Praxis</subfield><subfield code="v">22</subfield><subfield code="w">(DE-604)BV035709535</subfield><subfield code="9">22</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028932644&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-028932644</subfield></datafield></record></collection>
genre	(DE-588)4113937-9 Hochschulschrift gnd-content
genre_facet	Hochschulschrift
id	DE-604.BV043516604
illustrated	Not Illustrated
indexdate	2024-12-20T17:38:25Z
institution	BVB
isbn	9783942742689
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-028932644
oclc_num	951537544
open_access_boolean
owner	DE-91S DE-BY-TUM DE-91 DE-BY-TUM DE-M49 DE-BY-TUM DE-12 DE-859
owner_facet	DE-91S DE-BY-TUM DE-91 DE-BY-TUM DE-M49 DE-BY-TUM DE-12 DE-859
physical	141 Seiten Diagramme
publishDate	2016
publishDateSearch	2016
publishDateSort	2016
publisher	Verlag Attenkofer
record_format	marc
series	Nachwachsende Rohstoffe in Forschung und Praxis
series2	Nachwachsende Rohstoffe in Forschung und Praxis
spellingShingle	Rühmann, Broder 1977- Development of a modular high throughput screening platform for microbial exopolysaccharide producers Nachwachsende Rohstoffe in Forschung und Praxis Polysaccharide (DE-588)4131871-7 gnd High throughput screening (DE-588)4596131-1 gnd Bakterien (DE-588)4004296-0 gnd
subject_GND	(DE-588)4131871-7 (DE-588)4596131-1 (DE-588)4004296-0 (DE-588)4113937-9
title	Development of a modular high throughput screening platform for microbial exopolysaccharide producers
title_auth	Development of a modular high throughput screening platform for microbial exopolysaccharide producers
title_exact_search	Development of a modular high throughput screening platform for microbial exopolysaccharide producers
title_full	Development of a modular high throughput screening platform for microbial exopolysaccharide producers Broder Rühmann
title_fullStr	Development of a modular high throughput screening platform for microbial exopolysaccharide producers Broder Rühmann
title_full_unstemmed	Development of a modular high throughput screening platform for microbial exopolysaccharide producers Broder Rühmann
title_short	Development of a modular high throughput screening platform for microbial exopolysaccharide producers
title_sort	development of a modular high throughput screening platform for microbial exopolysaccharide producers
topic	Polysaccharide (DE-588)4131871-7 gnd High throughput screening (DE-588)4596131-1 gnd Bakterien (DE-588)4004296-0 gnd
topic_facet	Polysaccharide High throughput screening Bakterien Hochschulschrift
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028932644&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link	(DE-604)BV035709535
work_keys_str_mv	AT ruhmannbroder developmentofamodularhighthroughputscreeningplatformformicrobialexopolysaccharideproducers

Availability

Order (Login required)

Table of Contents
Order paper/chapter scan

Closed Stacks

Holdings details from Bibliotheksmagazin
Call Number:	0001 DM 34061 Floor plan
Copy 1	Available for loan On Shelf

Branch Library Weihenstephan

Holdings details from Teilbibliothek Weihenstephan
Call Number:	1002 CIT 960d 2016 A 2098 Floor plan
Copy 1	Available for loan On Shelf

Branch Library Straubing

Holdings details from Teilbibliothek Straubing
Call Number:	1302 ERG 700f 2009 A 6650-22 Floor plan 1304 ERG 700f 2009 A 6650-22 Floor plan
Copy 1	Available for loan On Shelf
Copy 1	Not available for loan On Shelf