Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia: = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Erlangen ; Nürnberg
2016
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028903736&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | Aus: PLoS Genetics ; 9. 2013 unter dem Titel: The Transcription Factors Sox10 and Myrf Define an Essential Regulatory Network Module in Differentiating Oligodendrocytes / Julia Hornig, Franziska Fröb ... |
| Umfang: | 124, 22 Seiten Illustrationen, Diagramme |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV043487124 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t| | ||
| 008 | 160331s2016 xx a||| m||| 00||| eng d | ||
| 035 | |a (OCoLC)951068053 | ||
| 035 | |a (DE-599)BVBBV043487124 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-29 |a DE-29T | ||
| 100 | 1 | |a Fröb, Franziska |d 1987- |e Verfasser |0 (DE-588)1095140647 |4 aut | |
| 245 | 1 | 0 | |a Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia |b = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus |c vorgelegt von Franziska Fröb |
| 246 | 1 | 1 | |a Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus |
| 264 | 1 | |a Erlangen ; Nürnberg |c 2016 | |
| 300 | |a 124, 22 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Aus: PLoS Genetics ; 9. 2013 unter dem Titel: The Transcription Factors Sox10 and Myrf Define an Essential Regulatory Network Module in Differentiating Oligodendrocytes / Julia Hornig, Franziska Fröb ... | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2016 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028903736&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-028903736 | |
Datensatz im Suchindex
| _version_ | 1819262194968166400 |
|---|---|
| adam_text | INTERPLAY OF TRANSCRIPTION FACTORS SOXLO AND
MYRF AND THE CHROMATIN REMODELER P400
IN MOUSE MYELINATING GLIA
DAS ZUSAMMENSPIEL DER TRANSKRIPTIONSFAKTOREN SOXLO UND
M YRF UND DES CHROMATIN-REMODELLIERERS P400
IN MYELINISIERENDEN GLIAZELLEN DER MAUS
DER NATURWISSENSCHAFTLICHEN FAKULTAET
DER FRIEDRICH-ALEXANDER-UNIVERSITAET ERLANGEN-NUERNBERG
ZUR
ERLANGUNG DES DOKTORGRADES DR. RER. NAT.
VORGELEGT VON
FRANZISKA FROEB
AUS ZEULENRODA
CONTENTS
CONTENTS
................
LIST OF FIGURES
......
LIST OF TABLES.
.......
ZUSAMMENFASSUNG
SUMMARY
1
INTRODUCTION.................................................................................................................
1.1 MYELINATING GLIA IN THE NERVOUS
SYSTEM.........................................................
1.1.1 DEVELOPMENT OF MYELINATING GLIA IN THE CENTRAL NERVOUS
SYSTEM...................
1.1.1.1 NEURULATION AND NEURAL
TUBE...........................................................................
1.1.1.2 OLIGODENDROCYTE
DEVELOPMENT........................................................................
1.1.2 DEVELOPMENT OF MYELINATING GLIA IN THE PERIPHERAL NERVOUS SYSTEM
.............
1.1.2.1 NEURAL CREST AND ITS
DERIVATIVES......................................................................
1.1.2.2 SCHWANN CELL
DEVELOPMENT..............................................................................
1.2 SOX
PROTEINS.....................................................................................................
1.2.1 SOXE
PROTEINS.................................................................................................
1.2.1.1
SOX8.................................................................................................................
1.2.1.2
SOX9.................................................................................................................
1.2.1.3 SOX
10...............................................................................................................
1.3
CHROMATIN.......................................................................................................
1.3.1 CHROMATIN REMODELING COMPLEXES
................................................................
1.3.1.1 SOX 10 AND CHROMATIN REMODELING
.................................................................
2 AIM OF THE
STUDY..........................................................................................................
3
RESULTS.........................................................................................................................
3.1 MYRF AS DIRECT TARGET GENE OF SOX 10 IN OLIGODENDROCYTES
.............................
3.1.1 EXPRESSION OF MYRF IN OLIGODENDROCYTES DEPENDS ON SOX 10
........................
3.1.2 REGULATORY REGIONS WITHIN THE MYRF GENE
....................................................
3.1.3 REGULATORY POTENTIAL AND SOX 10-RESPONSIVENESS OF MYRF ECR
....................
3.1.4 BINDING OF SOX 10 TO ECR9 IN
OLIGODENDROCYTES...........................................
3.1.5 SOX-BINDING SITES WITHIN
ECR9.......................................................................
3.1.6 FUNCTIONAL RELEVANT BINDING OF SOX 10 AS MONOMER AND DIMER WITHIN
ECR9
..2
.6
..7
..8
10
11
11
11
11
12
15
15
17
20
21
22
23
24
26
26
29
.32
.33
33
.34
.35
.36
.38
.39
.40
3.1.7 SOX8 BINDS TO ECR9
IN VITRO
BUT SITES ARE OCCUPIED BY SOX 10
IN VIVO
.................................43
3.2 FUNCTION OF P400 IN SCHWANN CELLS OF THE PN S
...................................................................
45
3.2.1 ANALYSIS OF THE INTERACTION OF SOX 10 WITH
P400....................................................................46
3.2.2 PHENOTYPIC ANALYSIS OF DHH::CRE-MEDIATED DELETION OF P400 IN
MICE.................................48
3.2.3 ULTRASTRUCTURAL AND MORPHOMETRIC ANALYSES OF P400-DEFICIENT
SCIATIC NERVES
......................
49
3.2.4 HISTOLOGICAL ANALYSIS OF MYELINATION IN P400-DEFICIENT SCIATIC
NERVES................................51
3.2.5 CHARACTERIZATION OF AN ALTERED EXPRESSION OF SCHWANN CELL
DIFFERENTIATION MARKERS IN P400-
DEFICIENT SCIATIC
NERVES.........................................................................................................53
3.2.6 ANALYSIS OF SCHWANN CELL DIFFERENTIATION MARKERS AND MYELIN
SPECIFIC GENES IN P400-
DEFICIENT SCIATIC NERVES ON RNA
LEVEL..................................................................................55
3.2.7 ANALYSIS OF PROLIFERATION, APOPTOSIS AND IMMUNE CELL INFILTRATION
IN P400-DEFICIENT SCIATIC
NERVES...................................................................................................................................56
4
DISCUSSION...........................................................................................................................................59
4.1 FEED-FORWARD MECHANISM OF SOX 10 AND MYRF IN THE CNS
..................................................
59
4.1.1 EXPRESSION OF MYRF AND MYELIN GENES IN OLIGODENDROCYTES DEPENDS ON
SOX 10...................60
4.1.2 INDUCTION OF MYRF EXPRESSION BY SOX 10 IS
ECR9-DEPENDENT..............................................60
4.1.3 PARTIAL REDUNDANCY OF SOX8 AND SOX 10 IN THE INDUCTION OF MYRF
EXPRESSION.......................62
4.2 ROLE OF P400 IN GLIAL CELLS OF THE PN S
.................................................................................
63
4.2.1 ROLE OF P400 IN SC
DEVELOPMENT.........................................................................................
64
4.2.2 ROLE OF P400 IN MYELIN FORMING
CELLS..................................................................................66
4.2.3 TIME-DEPENDENT SC MARKER GENE EXPRESSION UNDER LOSS OF P400
.......................................
67
4.2.4 IMPACT OF THE IMMUNE CELL INCREASE IN P400-DEFICIENT
NERVES.............................................67
4.2.5 INTERACTION OF SOX 10 AND
P400.............................................................................................68
5 MATERIAL AND
METHODS.......................................................................................................................71
5.1
MATERIAL................................................................................................................................71
5.1.1
ORGANISMS...........................................................................................................................
71
5.1.1.1 MOUSE
LINES...........................................................................................................................
71
5.1.1.2 CELL
LINES...............................................................................................................................71
5.1.1.3 PRIMARY
CELLS.........................................................................................................................
72
5.1.1.4 BACTERIAL
STRAINS..........................................................................................
72
5.1.2 CHEMICALS AND GENERAL REAGENTS
..........................................................................................
72
5.1.3 BUFFERS AND
SOLUTIONS...........................................................................................................72
5.1.4
ENZYMES..............................................................................................................................
77
5.1.5
OLIGONUCLEOTIDES.................................................................................................................
78
5.1.5.1 OLIGONUCLEOTIDES FOR
GENOTYPING...........................................................................................
78
5.1.5.2 OLIGONUCLEOTIDES FOR
CLONING...............................................................................................
78
5.1.5.3 OLIGONUCLEOTIDES FOR
EMSA................................................................................................
79
5.1.5.4 OLIGONUCLEOTIDES FOR
MUTAGENESIS........................................................................................
80
5.1.5.5 OLIGONUCLEOTIDES FOR
CHLP....................................................................................................
81
5.1.5.6 OLIGONUCLEOTIDES FOR
QRT-PCR............................................................................................
82
5.1.6
ANTIBODIES...........................................................................................................................
82
5.1.6.1 PRIMARY
ANTIBODIES................................................................................................................82
5.1.6.2 SECONDARY
ANTIBODIES..........................................................................................................83
5.2
METHODS................................................................................................................................84
5.2.1 ANIMAL
HUSBANDRY...............................................................................................................
84
5.2.2 STANDARD
METHODS.................................................................................................................84
5.2.3 GENOTYPING, MUTAGENESIS, CLONING,
QRT-PCR.....................................................................84
5.2.3.1
GENOTYPING...........................................................................................................................
84
5.2.3.1.1 ISOLATION OF GENOMIC DNA FOR
GENOTYPING...........................................................................
84
5.2.3.1.2 PCR FOR
GENOTYPING..............................................................................................................
85
5.2.3.2 SITE-DIRECTED
MUTAGENESIS......................................................................................................86
5.2.3.3 CLONING OF DNA
FRAGMENTS..................................................................................................
87
5.2.3.4 QUANTITATIVE
RT-PCR...........................................................................................................
88
5.2.3.4.1 REVERSE
TRANSCRIPTION............................................................................................................
88
5.2.3.4.2
QRT-PCR...............................................................................................................................88
5.2.4 HISTOLOGICAL
METHODS...........................................................................................................
89
5.2.4.1 TISSUE
PREPARATION.................................................................................................................89
5.2.4.1.1 TISSUE PREPARATION FOR
IMMUNOHISTOCHEMISTRY.....................................................................
89
5.2.4.1.2 TISSUE PREPARATION FOR PPD STAINING AND ELECTRON MICROSCOPY
.............................................
89
5.2.4.2
IMMUNOHISTOCHEMISTRY........................................................................................................
90
5.2.4.3 TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE DUTP NICK-END LABELING
(TUNEL)
.......................
90
5.2.4.5 PPD STAINING AND ELECTRON
MICROSCOPY................................................................................91
5.2.5 CELL CULTURE
METHODS............................................................................................................
91
5.2.5.1 CULTIVATION OF EUKARYOTIC
CELLS..............................................................................................91
5.2.5.2 TRANSFECTION OF HEK293
CELLS..............................................................................................91
5.2:5.3 PREPARATION OF WHOLE CELL
EXTRACTS........................................................................................
91
5.2.5.4 TRANSFECTION OF S16
CELLS.......................................................................................................92
5.2.5.5 LUCIFERASE REPORTER GENE
ASSAY.............................................................................................92
5.2.5.6 TRANSFECTION OF PRIMARY
OPCS.............................................................................................92
5.2.5.7
IMMUNOCYTOCHEMISTRY.........................................................................................................92
5.2.6 PROTEIN-BIOCHEMICAL
METHODS..............................................................................................93
5.2.6.1
SDS-PAGE............................................................................................................................93
5.2.6.2 WESTERN BLOT AND PROTEIN
DETECTION.......................................................................................93
5.2.6.3
GST-PULLDOWN.....................................................................................................................94
5.2.6.4 ELECTROMOBILITY SHIFT ASSAY (EMSA )
...................................................................................
95
5.2.O.5 CHROMATIN
IMMUNOPRECIPITATION..........................................................................................
95
5.2.7 IMAGE EDITING AND STATISTICAL
ANALYSIS...................................................................................97
ABBREVIATIONS.......................................................................................................................................
98
BIBLIOGRAPHY.......................................................................................................................................103
PUBLICATIONS AND
PRESENTATIONS.........................................................................................................121
LEBENSLAUF...........................................................................................................................................122
DANKSAGUNG.........................................................................................................................................123
ATTACHMENT.
124
|
| any_adam_object | 1 |
| author | Fröb, Franziska 1987- |
| author_GND | (DE-588)1095140647 |
| author_facet | Fröb, Franziska 1987- |
| author_role | aut |
| author_sort | Fröb, Franziska 1987- |
| author_variant | f f ff |
| building | Verbundindex |
| bvnumber | BV043487124 |
| ctrlnum | (OCoLC)951068053 (DE-599)BVBBV043487124 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01720nam a2200301 c 4500</leader><controlfield tag="001">BV043487124</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">160331s2016 xx a||| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)951068053</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV043487124</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29</subfield><subfield code="a">DE-29T</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Fröb, Franziska</subfield><subfield code="d">1987-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1095140647</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia</subfield><subfield code="b">= Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus</subfield><subfield code="c">vorgelegt von Franziska Fröb</subfield></datafield><datafield tag="246" ind1="1" ind2="1"><subfield code="a">Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Erlangen ; Nürnberg</subfield><subfield code="c">2016</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">124, 22 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Aus: PLoS Genetics ; 9. 2013 unter dem Titel: The Transcription Factors Sox10 and Myrf Define an Essential Regulatory Network Module in Differentiating Oligodendrocytes / Julia Hornig, Franziska Fröb ...</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Friedrich-Alexander-Universität Erlangen-Nürnberg</subfield><subfield code="d">2016</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028903736&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-028903736</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV043487124 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T17:37:36Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028903736 |
| oclc_num | 951068053 |
| open_access_boolean | |
| owner | DE-29 DE-29T |
| owner_facet | DE-29 DE-29T |
| physical | 124, 22 Seiten Illustrationen, Diagramme |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
| record_format | marc |
| spellingShingle | Fröb, Franziska 1987- Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus |
| subject_GND | (DE-588)4113937-9 |
| title | Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus |
| title_alt | Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus |
| title_auth | Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus |
| title_exact_search | Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus |
| title_full | Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus vorgelegt von Franziska Fröb |
| title_fullStr | Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus vorgelegt von Franziska Fröb |
| title_full_unstemmed | Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus vorgelegt von Franziska Fröb |
| title_short | Interplay of transcription factors Sox10 and Myrf and the chromatin remodeler p400 in mouse myelinating glia |
| title_sort | interplay of transcription factors sox10 and myrf and the chromatin remodeler p400 in mouse myelinating glia das zusammenspiel der transkriptionsfaktoren sox10 und myrf und des chromatin remodellierers p400 in myelinisierenden gliazellen der maus |
| title_sub | = Das Zusammenspiel der Transkriptionsfaktoren Sox10 und Myrf und des Chromatin-Remodellierers p400 in myelinisierenden Gliazellen der Maus |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028903736&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT frobfranziska interplayoftranscriptionfactorssox10andmyrfandthechromatinremodelerp400inmousemyelinatinggliadaszusammenspieldertranskriptionsfaktorensox10undmyrfunddeschromatinremodellierersp400inmyelinisierendengliazellendermaus AT frobfranziska daszusammenspieldertranskriptionsfaktorensox10undmyrfunddeschromatinremodellierersp400inmyelinisierendengliazellendermaus |