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Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae:

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Bibliographic Details
Main Author:	Müller, Sebastian (Author)
Format:	Thesis/Dissertation Book
Language:	English
Published:	2015
Subjects:	Hochschulschrift
Links:	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028030769&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Physical Description:	144, XXXVII S. Ill., graph. Darst.

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Record in the Search Index

DE-BY-TUM_call_number	0027 Diss.1 1288
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adam_text	TABLE OF CONTENTS TABLE OF CONTENTS ABBREVIATIONS XI 1 INTRODUCTION 15 1.1 THE WESTERN HONEY BEE 15 1.1.1 ECONOMICAL AND ECOLOGICAL IMPACT 16 1.2 PAENIBACILLUS LARVAE AND THE AMERICAN FOULBROOD 17 1.2.1 PAENIBACILLUS LARVAE CLASSIFICATION 18 1.2.2 PAENIBACILLUS LARVAE VIRULENCE 19 1.2.B THE GENOME OF PAENIBACILLUS LARVAE 21 1.3 NON-RIBOSOMALLY SYNTHESIZED SECONDARY METABOLITES 22 1.3.1 MECHANISMS OF SELF-RESISTANCE OF ANTIBIOTIC-PRODUCING BACTERIA 25 1.3.2 THE NON-RIBOSOMAL PEPTIDE SYNTHETASE (NRPS)-BASED BIOSYNTHESIS 26 1.3.3 THE POLYKETIDE SYNTHASE (PKS)-BASED BIOSYNTHESIS 32 1.3.4 NRPS/PKS HYBRIDS 35 1.3.5 SIDEROPHORES 36 1.3.6 NON-RIBOSOMALLY SYNTHESIZED SECONDARY METABOLITES OF THE GENUS PAENIBACILLUS.. 37 1.3.7 SECONDARY METABOLITES AS VIRULENCE FACTORS 38 2 AIM OF THIS WORK 41 3 MATERIALS 43 3.1 SOFTWARE 43 3.2 LABORATORY EQUIPMENT 43 3.3 CHEMICALS, ENZYMES, KITS AND CONSUMABLES 44 3.4 ENZYMES 45 3.5 OLIGONUCLEOTIDES 46 3.6 PLASMIDS 46 3.7 MICROORGANISMS 46 3.8 CULTURE MEDIA 47 HTTP://D-NB.INFO/1074218795 TABLE OF CONTENTS 3.9 BUFFERS AND SOLUTIONS 49 3.9.2 BUFFERS FOR SDS GLYCINE GEL ELECTROPHORESIS 49 3.9.3 BUFFER FOR PREPARATION OF ELECTROCOMPETENT B. SUBTILIS 50 3.9.4 BUFFERS FOR PROTEIN PURIFICATION 50 4 METHODS 51 4.1 MICROBIOLOGY 51 4.1.1 BACTERIAL STRAINS AND GENERAL CULTIVATION PROTOCOL 51 4.1.2 AGAR DIFFUSION ASSAY FOR ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY 52 4.1.3 CRYO CONSERVATION 52 4.2 MOLECULAR BIOLOGY 53 4.2.1 ISOLATION OF PLASMID DNAFROM BACTERIAL CELLS 53 4.2.2 ISOLATION OF GENOMIC DNA FROM P. LARVAE 53 4.2.3 POLYMERASE CHAIN REACTION (PCR) 53 4.2.4 COLONY POLYMERASE CHAIN REACTION (COLONY PCR) 55 4.2.5 DNA PURIFICATION 56 4.2.6 AGAROSE GEL ELECTROPHORESIS OF DNA 56 4.2.7 ISOLATION OF DNA FROM AGAROSE GELS 57 4.2.8 DNA RESTRICTION ENDONUCLEASE DIGEST 57 4.2.9 DNA LIGATION WITH T4 DNA LIGASE 57 4.2.14 DNA SEQUENCING 59 4.2.15 CONSTRUCTION OF P. LARVAE GENE INACTIVATION MUTANTS 59 4.3 PROTEIN BIOCHEMISTRY 59 4.3.1 EXPRESSION AND PURIFICATION OF PAMZ 59 4.3.2 IN VITRO ACTIVITY ASSAY OF PAMZ 60 4.3.3 IN VIVO ACTIVITY ASSAY OF PAMZ 60 4.4 NATURAL PRODUCT PURIFICATION 61 4.4.1 PURIFICATION OF SEVADICIN 61 4.4.2 PURIFICATION OF PAENILAMICIN 62 4.5 ANALYTICAL METHODS 63 4.5.1 ANALYTICS FOR SEVADICIN 63 4.5.2 ANALYTICS FOR PAENILAMICIN 66 4.5.3 ANALYTICS FOR BACILLIBACTIN 70 TABLE OF CONTENTS 4.6 CHEMISTRY METHODS 71 .4.6.1 SYNTHESIS OF SEVADICIN 71 4.7 IN SILICO ANALYSIS 72 5 RESULTS AND DISCUSSION 75 5.1 BIOACTIVITY OF P. LARVAE SECRETOMES 75 5.1.1 ANTIBACTERIAL ACTIVITY 75 5.1.2 ANTIFUNGAL ACTIVITY 77 5.2 SEVADICIN 77 5.2.1 BIOINFORMATICS 77 5.2.2 CHARACTERIZATION OF A ASEV GENE INACTIVATION MUTANT 80 5.2.3 ISOLATION AND STRUCTURE ELUCIDATION 80 5.2.4 BIOACTIVITY OF SEVADICIN 86 5.2.5 SEVADICIN IN BEE LARVAE 88 5.3 BACILLIBACTIN 89 5.3.1 BIOINFORMATICS 90 5.3.2 IDENTIFICATION AND STRUCTURE ELUCIDATION OF BACILLIBACTIN 91 5.3.3 ROLE IN PATHOGENESIS 95 5.4 PAENILAMICIN 98 5.4.1 BIOINFORMATICS 98 5.4.2 CHARACTERIZATION OF THE GENE INACTIVATION MUTANT 103 5.4.3 IDENTIFICATION, ISOLATION AND STRUCTURE ELUCIDATION OF PAENILAMICIN 105 5.4.4 BIOACTIVITY OF PAENILAMICIN 119 5.4.5 EFFECTS OF PAENILAMICIN IN BEE LARVAE 123 5.4.6 CHARACTERIZATION OF THE ACETYLTRANSFERASE PAMZ 126 5.5 PAENILARVINS 131 5.5.1 BIOINFORMATICS 131 5.5.2 STRUCTURE AND BIOACTIVITIES 132 5.6 A CRYPTIC MONOMODULAR NRPS-LIKE GENE CLUSTER 135 5.7 GENE CLUSTERS OF RIBOSOMALLY SYNTHESIZED PEPTIDES 136 6 SYNOPSIS AND OUTLOOK 141 TABLE OF CONTENTS 7 APPENDIX VII 8 REFERENCES XIII LIST OF FIGURES XXXI LIST OF TABLES XXXV DANKSAGUNG XXXVII
any_adam_object	1
author	Müller, Sebastian
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dewey-hundreds	600 - Technology (Applied sciences)
dewey-ones	638 - Insect culture
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dewey-search	638.1532
dewey-sort	3638.1532
dewey-tens	630 - Agriculture and related technologies
discipline	Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau
format	Thesis Book
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spellingShingle	Müller, Sebastian Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae
subject_GND	(DE-588)4113937-9
title	Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae
title_auth	Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae
title_exact_search	Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae
title_full	Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae vorgelegt von Sebastian Müller
title_fullStr	Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae vorgelegt von Sebastian Müller
title_full_unstemmed	Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae vorgelegt von Sebastian Müller
title_short	Structure elucidation and biosynthetic investigations of non-ribosomally synthesized secondary metabolites from the bee pathogenic bacterium Paenibacillus larvae
title_sort	structure elucidation and biosynthetic investigations of non ribosomally synthesized secondary metabolites from the bee pathogenic bacterium paenibacillus larvae
topic_facet	Hochschulschrift
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028030769&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT mullersebastian structureelucidationandbiosyntheticinvestigationsofnonribosomallysynthesizedsecondarymetabolitesfromthebeepathogenicbacteriumpaenibacilluslarvae

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Call Number:	0027 Diss.1 1288 Floor plan
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