Verfügbarkeit: Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells

Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells:

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Bibliographische Detailangaben
Beteilige Person:	Gräf, Patricia (VerfasserIn)
Format:	Hochschulschrift/Dissertation Buch
Sprache:	Englisch
Veröffentlicht:	München Verl. Dr. Hut 2015
Ausgabe:	1. Aufl.
Schriftenreihe:	Medizin
Schlagwörter:	Serial transfer immunocompetence central memory T cells Hochschulschrift
Links:	http://deposit.dnb.de/cgi-bin/dokserv?id=5206461&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028005588&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Umfang:	XV, 152 S. Ill., graph. Darst. 210 mm x 148 mm, 354 g
ISBN:	9783843920643 3843920648

Internformat

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Datensatz im Suchindex

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adam_text	CONTENTS INDEX OF FIGURES VII ABBREVIATIONS XI 1INTRODUCTION 1 1.1 THE IMMUNE SYSTEM 1 1.1.1 T CELL IMMUNITY 3 1.1.2 THE HETEROGENEITY OF CD8* CYTOTOXIC T CELLS, SUBSETS AND THE DIVISION OF LABOR. 4 1.1.3 THE FIINCTIONAL AND DEVELOPMENTAL CAPACITIES OF MEMORY T CELL SUBSETS... 8 1.2 T CELL DIFFERENTIATION, LLNEAGE RELATION AND ANALOGY TO TISSUE STEMNESS 10 1.2.1 MODELS OF CD8* T CELL DIFFERENTIATION 10 1.2.2 INTRINSIC VARIABILITY AND ENVIRONMENTAL CUES_ - 13 1.2.3 ANALOGY TO HOMEOSTASIS IN OTHER CYCLING TISSUES 16 1.2.4 THE CONCEPT OF T CELL TISSUE STEM CELLS 18 1.3 ADOPTIVE T CELL THERAPY 22 1.3.1 T CELL THERAPY IN VIRAL INFECTIONS 22 1.3.2 T CELL THERAPY IN CANCER 24 1.3.3 FINDING THE RIGHT TARGET POPULATION FOR T CELL THERAPY 25 2 AIM OF THIS PHD THESIS 27 3 MATERIALS AND METHODS 29 3.1 MATERIALS 29 3.1.1 EQUIPMENT _ 29 3.1.2 CHEMICALS AND REAGENTS 30 3.1.3 BUFFERS AND MEDIA 32 3.1.4 ANTIBODIES 33 3.1.5 PEPTIDES 34 3.1.6 MHC MULUEMERS 35 3.1.7 MICE..._ 35 3.1.8 SOFTWARE -35 III HTTP://D-NB.INFO/1076497527 CONTENTS 3.2 METHODS 36 3.2.1 PREPARATION OF CELLS FROM DIFFERENT ORGANS 36 3.2.2 PRODUCTION OF MHC-1 MULTIMERS .. 37 3.2.3 MULTIMERIZATION 37 3.2.4 ANTIBODY AND MULTIMER STAINING............................................................... . 37 3.2.5 INTRACELLULAR ANTIBODY STAINING 37 3.2.6 FACS ACQUISITUM AND ANALYSIS 38 3.2.7 FACS SORTING 38 3.2.7.1 PURIFLCATION OF NAIVE CD8* T CELLS 39 3.2.7.2 PURIFLCATION OF MEMORY CD8+ T CELLS 39 3.2.8 ADOPTIVE CELL TRANSFERS 40 3.2.9 INFECTIONS AND IMMUNIZATIONS 40 3.2.9.1 LISTERIA MONOCYTOGENES INFECTION 40 3.2.9.2 MODIFIED VACCINIA VIRUS ANKARA IMMUNIZATION.... 40 3.2.10 DETERMINATION OF BACTERIAL BUERDEN 41 3.2.11 STATISTICS 41 4 RESULTS 43 4.1 LONG-UVED MEMORY AFTER ACUTE INFECTION CONSISTS OF RESTING CD8+ CD44 W * H CENTRAL MEMORY AND EFFECTOR MEMORY T CELLS 4.2 EVALUATION OF THE DEVELOPMENTAL CAPACITY OF CD8+ CENTRAL MEMORY AND EFFECTOR MEMORY T CELLS ..48 4.2.1 ESTABLISHMENT OF AN OPTIMAL STAINING PROCEDURA TO PURIFY ANTIGEN-SPECIFIC CD8* MEMORY T CELLS FOR ADOPTIVE RE^TRANSFER............................................... ............. 48 4.2.2 HIGH EXPANSION AND DIFFERENTIATION POTENTIAL OF CD8* CENTRAL MEMORY T CELLS 52 4.3 EXTENDLNG THE SINGLE CELL ADOPTIVE TRANSFER TO CD8* CENTRAL MEMORY T CELLS ...................56 4.3.1 ESTABLISHMENTOFA CONGENIC MATRIXOFCD8+ CENTRAL MEMORY T CELLS.. 57 4.3.2 FURTHER EVALUATION OF AN OPTIMAL STAINING PROCEDURA TO PURIFY CD8+ CENTRAL MEMORY T CELLS FOR ADOPTIVE RE-TRANSFER ... ... 61 4.3.3 SENSITIVITY OF CD8+ EFFECTOR AND MEMORY T CELLS TO MEMORY MARKER STAINING 66 4.3.4 SIMILAR DEVELOPMENTAL CAPACITY OF CD8* NAIVE AND CENTRAL MEMORY T CELLS 69 4.3.5 HIGH EXPANSION AND DIFFERENTIATION CAPACITY OF CD8* CENTRAL MEMORY T CELLS FROM DIVERSE ANATOMICAL ORIGIN 71 4.4 PRESERVATION OF KEY DEVELOPMENTAL TRAITS BETWEEN SINGLE CDS* NAIVE AND CENTRAL MEMORY T CELLS 75 IV CONTENTS 4.5 SERIAL ADOPTIVE TRANSFER AND INFECTION-DRIVEN RE-EXPANSION OF SINGLE CELL-DERIVED IMMUNO- COMPETENCE REVEALS STEMNESS OF INDIVIDUAL CDS 4 CENTRAL MEMORY T CELLS 79 4.5.1 ESTABLISHMENT OF TRANS-GENERATIONAL SINGLE CELL ADOPTIVE TRANSFERS - 79 4.5.2 SERIAL ADOPTIVE TRANSFER OF SINGLE CELLS DEMONSTRATES FUNCTIONAL SELF-RENEWAL CAPACITY OF INDIVIDUAL CD8* CENTRAL MEMORY T CELLS 83 4.5.3 SINGLE CD8* CENTRAL MEMOIY T CELLS REMAIN MULTI-POTENT THROUGHOUT SERIAL ADOPTIVE TRANSFERS 87 4.5.4 PROGENY DERIVED FROM SINGLE CD8* CENTRAL MEMORY T CELLS CAN RESTORE IMMUNO-COMPETENCE 90 5 DISCUSSION 95 5.1 FIRST FUNCTIONAL EVIDENCE FOR STEMNESS IN THE T CELL POOL 97 5.2 THE UNFORESEEN PRESERVATION OF KEY DEVELOPMENTAL TRALTS BETWEEN CD8* NAIVE AND CENTRAL MEMORY T CELLS 100 5.3 THE IMPACT ON T CELL DIFFERENTIATION AND MEMORY RELLABILITY 104 5.4 FUTURE PERSPECTIVES * 108 5.5 CLINICAL RELEVANCE 112 6 SUMMARY 115 7 ACKNOWLEOGEMENTS 117 8 REFERENCES 119 V
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author	Gräf, Patricia
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edition	1. Aufl.
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physical	XV, 152 S. Ill., graph. Darst. 210 mm x 148 mm, 354 g
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publisher	Verl. Dr. Hut
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spellingShingle	Gräf, Patricia Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells
subject_GND	(DE-588)4113937-9
title	Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells
title_auth	Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells
title_exact_search	Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells
title_full	Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells Patricia Gräf
title_fullStr	Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells Patricia Gräf
title_full_unstemmed	Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells Patricia Gräf
title_short	Serial transfer of single cell-derived immunocompetence reveals stemness of CD8 + central memory T cells
title_sort	serial transfer of single cell derived immunocompetence reveals stemness of cd8 central memory t cells
topic_facet	Hochschulschrift
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