Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2011
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027729312&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | IV, 162 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV042292174 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t| | ||
| 008 | 150127s2011 xx ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)907953326 | ||
| 035 | |a (DE-599)BVBBV042292174 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-83 |a DE-91 | ||
| 082 | 0 | |a 660.65 |2 22//ger | |
| 100 | 1 | |a Krawczyk, Joanna Martyna |e Verfasser |0 (DE-588)1065675380 |4 aut | |
| 245 | 1 | 0 | |a Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches |c vorgelegt von Joanna Martyna Krawczyk |
| 264 | 1 | |c 2011 | |
| 300 | |a IV, 162 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Berlin, Techn. Univ., Diss., 2011 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027729312&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-027729312 | |
Datensatz im Suchindex
| DE-BY-TUM_call_number | 0001 DA 2015 10 |
|---|---|
| DE-BY-TUM_katkey | 2115798 |
| DE-BY-TUM_location | Mag |
| DE-BY-TUM_media_number | 040007905813 |
| _version_ | 1821935351410720769 |
| adam_text | TABLE OF CONTENTS
1. INTRODUCTION 1
1 1. LANTIBIOTIC 1
1 2. CLASS III LANTIBIOTICS 6
1 3. LABYRINTHOPEPTINS 11
2 AIMS OF THE STUDY 14
3. MATERIALS 15
3.1. LABORATORY EQUIPMENT 15
3.2. CHEMICALS AND ENZYMES 16
3.2.1. CHEMICALS 16
3.2.2. ENZYMES 19
3.3 ANTIBIOTICS 19
3.4 BACTERIAL STRAINS 20
3 5 KITS 20
3 6 ANTIBODIES 21
3 7 SOLUTIONS, BUFFERS AND MEDIA 21
3.7 1 MEDIA 21
3.7 2 SOLUTIONS, BUFFERS 23
3 8. PLASMIDS 28
3.9. OLIGONUCLEOTIDES AND SYNTHETIC GENES 29
3.10. SOFTWARE 36
4. METHODS 37
4.1. MICROBIOLOGICAL METHODS 37
4 1
1 GROWTH CONDITIONS AND PRESERVATION
OF E COLI 37
4.1.2. GROWTH CONDITIONS AND PRESERVATION OF STREPTOMYCES AND A.
NAMIBIENSIS....
37
4.2. MOLECULAR BIOLOGICAL METHODS 37
4 2
1 ISOLATION, PURIFICATION AND
ANALYSIS OF DNA 37
4.2.2. INTRODUCTION OF DNA
INTO E. COLI 40
4.2.3. INTRODUCTION OF DNA
INTO STREPTOMYCES AND A. NAMIBIENSIS 40
4 2
4. POLYMERASE CHAIN REACTION
(PCR) 43
4.2.5. SITE-DIRECTED MUTAGENESIS 44
4.2.6. CLONING EXPERIMENTS 45
4.2.7. SOUTHERN-BLOTTING 46
4.2.8. TRIS-GLYCINE SDS-PAGE AND WESTERN
BLOTTING 47
4 3. ANALYTICAL METHODS 49
4 31. SAMPLE PREPARATION FOR MS ANALYSIS 49
4 3 2. MASS SPECTROMETRY ANALYSIS OF LABYNNTHOPEPTIN VARIANTS 50
5. INTRODUCTION OF DNA
INTO A. NAMIBIENSIS 52
5.1. INTRODUCTION 52
5.2. RESULTS AND DISCUSSION 52
5.2.1. PLASMIDS 52
5.2.2. PEG-ASSISTED TRANSFORMATION OF A. NAMIBIENSIS PROTOPLASTS 54
5.2.3. INTERGENERIC CONJUGATION
BETWEEN E. COLI AND
A. NAMIBIENSIS 56
5.2.4. DIRECT TRANSFORMATION OF A
NAMIBIENSIS 57
5.2.5. ELECTROPORATION OF MYCELIUM 57
5 3 SUMMARY 58
6 HETEROLOGOUS EXPRESSION OF LABYRINTHOPEPTINS
IN STREPTOMYCES 59
6.1. INTRODUCTION 59
6 2. RESULTS AND DISCUSSION 60
6.2.1. HETEROLOGOUS EXPRESSION OF A COSMID
M1104 BEARING THE LAB GENE
CLUSTER.. 60
6.2.2. HETEROLOGOUS EXPRESSION OF LABYRINTHOPEPTIN BY USING
THE VECTOR PUWLAB...
62
6.3. SUMMARY ..70
HTTP://D-NB.INFO/1067122117
7 ESTABLISHING METHODS FOR EFFICIENT
GENERATION OF NOVEL
LABYRINTHOPEPTINS 71
7.1. INTRODUCTION... . . 71
7.2 RESULTS AND DISCUSSION 72
7.2.1. CONSTRUCTION OF THE VECTOR
PLAB FOR HETEROLOGOUS EXPRESSION
IN STREPTOMYCES
72
7 2
2 OPTIMIZATION OF A TEMPLATE VECTOR
FOR ENGINEERING OF LABA1 76
7.2.3. OPTIMIZATION OF A VECTOR TEMPLATE FOR ENGINEERING
OF LABA2 80
7 2
4. EXPRESSION OF A SINGLE
LABYRINTHOPEPTIN 82
7.3. SUMMARY 84
8. ENGINEERING OF NEW LABYNNTHOPEPTINS 85
8.1. INTRODUCTION 85
8.2. RESULTS AND DISCUSSION .... 87
8.2 1 STRATEGY FOR THE GENERATION OF MUTANTS 87
8.2 2. ALANINE-SCANNING MUTAGENESIS OF LABA1
AND LABA2 91
8.2.3. MODULATION OF THE SER/SER/CYS
MOTIF 94
8.2.4. SUBSTITUTION OF SER INVOLVED
IN LAB FORMATION
BY THR.. 99
8.2.5 EXPRESSION OF PARTIAL STRUCTURES OF LABA1 100
8.2 6 SER/SER/SER/CYS MOTIF 101
8.2.7 ADDITIONAL SUBSTITUTIONS . 102
8.2.8. STUDIES ON RING C MUTATIONS 105
8.2.9. INFLUENCE OF SPACER-AMINO
ACIDS ON LABYRINTHOPEPTIN SYNTHESIS
. 106
8 2
10. GENERATION OF LABYRINTHOPEPTIN HYBRIDS 107
8.3. SUMMARY 110
9 CONCLUSIONS AND FUTURE DIRECTIONS 114
LIST OF FIGURES 116
LIST OF TABLES . 124
APPENDIX (MAPS OF PLASMIDS, LC-MS
SPECTRA) 126
BIBLIOGRAPHY 152
II
|
| any_adam_object | 1 |
| author | Krawczyk, Joanna Martyna |
| author_GND | (DE-588)1065675380 |
| author_facet | Krawczyk, Joanna Martyna |
| author_role | aut |
| author_sort | Krawczyk, Joanna Martyna |
| author_variant | j m k jm jmk |
| building | Verbundindex |
| bvnumber | BV042292174 |
| ctrlnum | (OCoLC)907953326 (DE-599)BVBBV042292174 |
| dewey-full | 660.65 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.65 |
| dewey-search | 660.65 |
| dewey-sort | 3660.65 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01162nam a2200289 c 4500</leader><controlfield tag="001">BV042292174</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">150127s2011 xx ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)907953326</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV042292174</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-83</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">660.65</subfield><subfield code="2">22//ger</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Krawczyk, Joanna Martyna</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1065675380</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches</subfield><subfield code="c">vorgelegt von Joanna Martyna Krawczyk</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">IV, 162 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Berlin, Techn. Univ., Diss., 2011</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027729312&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-027729312</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV042292174 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T17:06:58Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027729312 |
| oclc_num | 907953326 |
| open_access_boolean | |
| owner | DE-83 DE-91 DE-BY-TUM |
| owner_facet | DE-83 DE-91 DE-BY-TUM |
| physical | IV, 162 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| record_format | marc |
| spellingShingle | Krawczyk, Joanna Martyna Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches |
| subject_GND | (DE-588)4113937-9 |
| title | Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches |
| title_auth | Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches |
| title_exact_search | Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches |
| title_full | Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches vorgelegt von Joanna Martyna Krawczyk |
| title_fullStr | Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches vorgelegt von Joanna Martyna Krawczyk |
| title_full_unstemmed | Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches vorgelegt von Joanna Martyna Krawczyk |
| title_short | Heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches |
| title_sort | heterologous expression and generation of labyrinthopeptin derivates by genetic engineering approaches |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027729312&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT krawczykjoannamartyna heterologousexpressionandgenerationoflabyrinthopeptinderivatesbygeneticengineeringapproaches |
Inhaltsverzeichnis
Paper/Kapitel scannen lassen
Paper/Kapitel scannen lassen
Bibliotheksmagazin
| Signatur: |
0001 DA 2015 10 Lageplan |
|---|---|
| Exemplar 1 | Ausleihbar Am Standort |