Mitochondrial regulation: methods and protocols
Gespeichert in:
| Weitere beteiligte Personen: | |
|---|---|
| Format: | Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2015
|
| Schriftenreihe: | Methods in molecular biology
1241 |
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027693924&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | Includes bibliographical references and index |
| Umfang: | XI, 201 S. Ill., graph. Darst. |
Internformat
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| 264 | 1 | |a New York, NY [u.a.] |b Humana Press |c 2015 | |
| 300 | |a XI, 201 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 1241 | |
| 490 | 0 | |a Springer protocols | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Mitochondria / Laboratory manuals | |
| 650 | 4 | |a Cellular control mechanisms / Laboratory manuals | |
| 650 | 4 | |a Cell physiology / Laboratory manuals | |
| 650 | 4 | |a Mitochondria | |
| 650 | 4 | |a Mitochondrial regulationchondria | |
| 700 | 1 | |a Palmeira, Carlos M. |4 edt | |
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Datensatz im Suchindex
| _version_ | 1819248053281882112 |
|---|---|
| adam_text | Titel: Mitochondrial regulation
Autor: Palmeira, Carlos M
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... ix
1 Isolation of Crude Mitochondrial Fraction from Cells................... 1
Mariusz R. Wieckowski and Lech Wojtczak
2 Isolation of Mitochondria from Liver and Extraction of Total RNA
and Protein: Analyses of MicroRNA and Protein Expressions............. 9
Pedro M. Borralho, Clifford J. Steer, and Cecilia M.P. Rodrigues
3 PCR Based Determination of Mitochondrial DNA Copy Number
in Multiple Species............................................. 23
John P. Rooney, Ian T. Ryde, Laurie H. Sanders, Evan H. Howlett,
Meryl D. Colton, Kaylyn E. Germ, Greg D. Mayer,
J. Timothy Greenamyre, and Joel N. Meyer
4 NAD* Content and Its Role in Mitochondria......................... 39
Wei Li and Anthony A. Sauve
5 Measuring PGC-la and Its Acetylation Status in Mouse Primary Myotubes. . . 49
Ana P. Gomes and David A. Sinclair
6 Measurement of Mitochondrial Oxygen Consumption Rates
in Mouse Primary Neurons and Astrocytes........................... 59
Sofia M. Ribeiro, Alfredo Giménez-Cassina, and Nika N. Danial
7 Determination of Oxidative Phosphorylation Complexes Activities......... 71
J odo S. Teodoro, Carlos M. Palmeira, and Anabela P. Rolo
8 Histoenzymatic Methods for Visualization of the Activity
of Individual Mitochondrial Respiratory Chain Complexes
in the Muscle Biopsies from Patients with Mitochondrial Defects.......... 85
Agnieszka Karkucinska-Wieckowska, MaciejPronicki,
and Mariusz R. Wieckowski
9 A Method Aimed at Assessing the Functional Consequences
of the Supramolecular Organization of the Respiratory Electron
Transfer Chain by Time-Resolved Studies............................ 95
Fabrice Rappaport
10 BN-PAGE-Based Approach to Study Thyroid Hormones
and Mitochondrial Function...................................... Ill
Elena Silvestri, Assunta Lombardi, Federica Cìoffi,
and Fernando Goglia
11 Detection of UCP1 Protein and Measurements of Dependent
GDP-Sensitive Proton Leak in Non-phosphorylating
Thymus Mitochondria.......................................... 123
KieranJ. Clarke, Audrey M. Carroll, Gemma O Brien,
and Richard K. Porter
Vii
viii
Contents
12 Exploring Liver Mitochondrial Function by 13C-Stable Isotope
Breath Tests: Implications in Clinical Biochemistry..................... 137
Ignazio Grattagliano, Leonilde Bonfrate, Michele Lorusso,
Luigi Castorani, Ornella de Bari, and Piero Portincasa
13 Following Mitochondria Dynamism: Confocal Analysis
of the Organelle Morphology..................................... 153
Francesca R. Mariotti, Mauro Corrado, and Silvia Campello
14 Analysis of Pro-apoptotic Protein Trafficking to and from Mitochondria..... 163
Ignacio Vega-Naredo, Teresa Cunha-Oliveira, Teresa L. Serafim,
Vilma A. Sardao, and Paulo J. Oliv eira
15 Mitophagy and Mitochondrial Balance.............................. 181
Simone Patergnani and Paolo Pinton
Index............................................................... 195
|
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| illustrated | Illustrated |
| indexdate | 2024-12-20T17:06:00Z |
| institution | BVB |
| language | English |
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| owner | DE-12 DE-11 |
| owner_facet | DE-12 DE-11 |
| physical | XI, 201 S. Ill., graph. Darst. |
| publishDate | 2015 |
| publishDateSearch | 2015 |
| publishDateSort | 2015 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spellingShingle | Mitochondrial regulation methods and protocols Methods in molecular biology Mitochondria / Laboratory manuals Cellular control mechanisms / Laboratory manuals Cell physiology / Laboratory manuals Mitochondria Mitochondrial regulationchondria |
| title | Mitochondrial regulation methods and protocols |
| title_auth | Mitochondrial regulation methods and protocols |
| title_exact_search | Mitochondrial regulation methods and protocols |
| title_full | Mitochondrial regulation methods and protocols Ed. by Carlos M. Palmeira |
| title_fullStr | Mitochondrial regulation methods and protocols Ed. by Carlos M. Palmeira |
| title_full_unstemmed | Mitochondrial regulation methods and protocols Ed. by Carlos M. Palmeira |
| title_short | Mitochondrial regulation |
| title_sort | mitochondrial regulation methods and protocols |
| title_sub | methods and protocols |
| topic | Mitochondria / Laboratory manuals Cellular control mechanisms / Laboratory manuals Cell physiology / Laboratory manuals Mitochondria Mitochondrial regulationchondria |
| topic_facet | Mitochondria / Laboratory manuals Cellular control mechanisms / Laboratory manuals Cell physiology / Laboratory manuals Mitochondria Mitochondrial regulationchondria |
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