Verfügbarkeit: Nucleophilic active site mimetics and methodological advances in activity-based protein profiling

Nucleophilic active site mimetics and methodological advances in activity-based protein profiling:

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Bibliographische Detailangaben
Beteilige Person:	Rudolf, Georg C. (VerfasserIn)
Format:	Hochschulschrift/Dissertation Buch
Sprache:	Englisch
Veröffentlicht:	München Verl. Dr. Hut 2014
Ausgabe:	1. Aufl.
Schriftenreihe:	Organische Chemie
Schlagwörter:	Markierung > Chemie Struktur-Aktivitäts-Beziehung Proteomanalyse Hochschulschrift
Links:	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027169480&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Umfang:	XV, 193 S. Ill., graph. Darst.
ISBN:	9783843914475

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Datensatz im Suchindex

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adam_text	I I TABLE OF CONTENTS 1 TABLE OF CONTENTS I 2 INTRODUCTORY REMARKS III 3 SUMMARY V 4 ZUSAMMENFASSUNG IX 5 LIST OF ABBREVIATIONS, ACRONYMS AND SYMBOLS XIV 1 INTRODUCTION L 1.1 ACTIVITY-BASED PROTEIN PROFILING L I . I . I BASIC PROCEDURES OF ABPP EXPERIMENTS 3 1.2 SCOPE OF THIS WORK 4 2 TRIFUNCTIONAL LINKER 2 ND GENERATION 7 2.1 INTRODUCTION 7 2.2 TRIFUNCTIONAL LINKERS: CATCHING, REPORTING & ENRICHMENT 8 2.3 RATIONAL DESIGN OF TRIFUNCTIONAL LINKER 2 ND GENERATION 9 2.4 SYNTHESIS OF TRIFUNCTIONAL LINKER 2 ND GENERATION 10 2.5 EVALUATION OFTFL-2 D IN THE ABPP PROCESS IT 3 CLEAVABLE LINKERS FOR IMMOBILIZATION AND ENRICHMENT 13 3.1 INTRODUCTION 13 3.2 CURRENT CLEAVAGE METHODS FOR PROTEIN MODIFICATIONS 14 3.2.1 CLEAVAGE OF THERMAL- AND PHOTOCLEAVABLE LINKERS 14 3.2.2 CLEAVAGE BY CHEMICAL REAGENTS: ACID- AND REDUCTION-BASED METHODS 15 3.3 RESULTS AND DISCUSSION 17 3.3.1 NOVEL APPROACH FOR BASE-CLEAVABLE LINKERS 17 3.3.2 SYNTHESES OF MODEL COMPOUNDS FOR PRELIMINARY EVALUATIONS 18 3.3.3 PRELIMINARY EVALUATION OF MODEL LINKERS 21 3.3.4 SYNTHESES OF PROTOTYPICAL LINKERS FOR ENRICHMENT PURPOSES 25 3.4 CONCLUDING REMARKS 27 4 ENHANCEMENT OF COPPER-ASSISTED CLICK REACTIONS FOR ACTIVITY-BASED PROTEOMICS 29 4.1 INTRODUCTION 29 4.2 RESULTS AND DISCUSSION 31 4.2.1 RATIONAL DESIGN AND SYNTHESES OF TAILORED LIGANDS 31 4.2.2 KINETIC ANALYSES AND OPTIMIZATION OF REACTION CONDITIONS 33 4.2.3 EVALUATION OF BIOORTHOGONAL CLICK REACTIONS FOR IN VITRO ABPP LABELLING 35 4.3 CONCLUDING REMARKS 40 5 NUCLEOPHILIC ACTIVE SITE MIMETICS 43 5.1 INTRODUCTION 43 5.2 DISCOVERY OF ELECTROPHILIC NATURAL PRODUCTS 44 5.2.1 ELECTROPHILIC NATURAL PRODUCTS 44 5.2.2 METHODS FOR THE IDENTIFICATION OF NATURAL PRODUCTS 45 HTTP://D-NB.INFO/1048241602 II 5.3 NUCLEOPHILIC ACTIVE SITE MIMETICS APPROACH 46 5.3.1 PRIVILEGED STRUCTURES FOR NASM PROBES 47 5.3.2 PROJECT OUTLINE FOR PROOF-OF-CONCEPT STUDY 48 5.4 RESULTS AND DISCUSSION 49 5.4.1 SYNTHESES OF NASM PROBES 49 5.4.2 EVALUATION OF NASM PROBES REACTIVITY UNDER BIOORTHOGONAL CONDITIONS 54 5.4.3 EVALUATION WITH MODEL SYSTEM SHOWDOMYCIN 60 5.5 CONCLUDING REMARKS AND OUTLOOK 64 6 EXPERIMENTAL SECTION 67 6.1 SYNTHETIC ORGANIC CHEMISTRY 67 6.1.1 GENERAL METHODS AND MATERIALS 67 6.1.2 SYNTHETIC PROCEDURES FOR CHAPTER 2: TRIFUNCTIONAL LINKER 2 ND GENERATION 69 6.1.3 SYNTHETIC PROCEDURES FOR CHAPTER 3: CLEAVABLE LINKERS FOR IMMOBILIZATION AND ENRICHMENT 71 6.1.4 SYNTHETIC PROCEDURES FOR CHAPTER 4: ENHANCEMENT OF COPPER-ASSISTED CLICK REACTIONS FOR ACTIVITY-BASED PROTEOMICS 85 6.1.5 SYNTHETIC PROCEDURES FOR CHAPTER 5: NUCLEOPHILIC ACTIVE SITE MIMETICS 100 6.1.6 SYNTHETIC PROCEDURES FOR STUDIES TOWARDS THE IDENTIFICATION OF ASP-PHOS MODIFICATIONS 116 6.2 BIOCHEMISTRY AND RELATED PROCEDURES 121 6.2.1 GENERAL METHODS AND MATERIALS 121 6.2.2 FLUORESCENCE-BASED KINETIC EXPERIMENTS OF CHAPTER 4 124 6.2.3 PROTEOMICS: PROCEDURES FOR ANALYTICAL IN VITRO LABELLING EXPERIMENTS OF CHAPTER 4 125 6.2.4 PROTEOMICS: PROCEDURES FOR PREPARATIVE LABELLING AND IDENTIFICATION EXPERIMENTS OF CHAPTER 4 127 6.2.5 REACTIVITY EVALUATION EXPERIMENTS OF CHAPTER 5 129 7 BIBLIOGRAPHY 131 8 CURRICULUM VITAE AND ACKNOWLEDGEMENTS 147 9 APPENDICES 151 9.1 APPENDIX FOR CHAPTER 4: ENHANCEMENT OF COPPER-ASSISTED CLICK REACTIONS FOR ACTIVITY-BASED PROTEOMICS 151 9.1.1 SELECTED H AND 13 C NMR SPECTRA 152 9.1.2 KINETIC ANALYSES AND OPTIMIZATION OF REACTION CONDITIONS 170 9.1.3 IN VITRO LABELLING EXPERIMENTS WITH B. SUBTILIS PROTEOME 175 9.2 APPENDIX FOR CHAPTER 5: NUCLEOPHILIC ACTIVE SITE MIMETICS 181 9.2.1 SELECTED H AND 13 C NMR SPECTRA 182
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indexdate	2024-12-20T16:53:32Z
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isbn	9783843914475
language	English
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physical	XV, 193 S. Ill., graph. Darst.
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series2	Organische Chemie
spellingShingle	Rudolf, Georg C. Nucleophilic active site mimetics and methodological advances in activity-based protein profiling Markierung Chemie (DE-588)4201779-8 gnd Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd Proteomanalyse (DE-588)4596545-6 gnd
subject_GND	(DE-588)4201779-8 (DE-588)4183784-8 (DE-588)4596545-6 (DE-588)4113937-9
title	Nucleophilic active site mimetics and methodological advances in activity-based protein profiling
title_auth	Nucleophilic active site mimetics and methodological advances in activity-based protein profiling
title_exact_search	Nucleophilic active site mimetics and methodological advances in activity-based protein profiling
title_full	Nucleophilic active site mimetics and methodological advances in activity-based protein profiling Georg Christoph Rudolf
title_fullStr	Nucleophilic active site mimetics and methodological advances in activity-based protein profiling Georg Christoph Rudolf
title_full_unstemmed	Nucleophilic active site mimetics and methodological advances in activity-based protein profiling Georg Christoph Rudolf
title_short	Nucleophilic active site mimetics and methodological advances in activity-based protein profiling
title_sort	nucleophilic active site mimetics and methodological advances in activity based protein profiling
topic	Markierung Chemie (DE-588)4201779-8 gnd Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd Proteomanalyse (DE-588)4596545-6 gnd
topic_facet	Markierung Chemie Struktur-Aktivitäts-Beziehung Proteomanalyse Hochschulschrift
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027169480&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT rudolfgeorgc nucleophilicactivesitemimeticsandmethodologicaladvancesinactivitybasedproteinprofiling

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