Availability: Function of the transcription factor Fra1 in adipogenesis

Function of the transcription factor Fra1 in adipogenesis: = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese

Saved in:

Bibliographic Details
Main Author:	Luther Julia (Author)
Format:	Thesis/Dissertation Book
Language:	English
Published:	2012
Subjects:	Fettgewebe Transkriptionsfaktor Fettsucht Hochschulschrift
Links:	https://open.fau.de/handle/openfau/2798 http://d-nb.info/1029374260/34 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-40161 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025745519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Physical Description:	106 S. graph. Darst.

Staff View

MARC


LEADER	00000nam a2200000zc 4500
001	BV040767062
003	DE-604
005	20150206
007	t\|
008	130221s2012 gw d\|\|\| m\|\|\| 00\|\|\| eng d
015			\|a 13,O02 \|2 dnb
016	7		\|a 1029374260 \|2 DE-101
035			\|a (OCoLC)828809888
035			\|a (DE-599)DNB1029374260
040			\|a DE-604 \|b ger \|e rakddb
041	0		\|a eng
044			\|a gw \|c XA-DE-BY
049			\|a DE-384 \|a DE-473 \|a DE-703 \|a DE-1051 \|a DE-824 \|a DE-29 \|a DE-12 \|a DE-91 \|a DE-19 \|a DE-1049 \|a DE-92 \|a DE-739 \|a DE-898 \|a DE-355 \|a DE-706 \|a DE-20 \|a DE-1102 \|a DE-29T
082	0		\|a 610
084			\|a 610 \|2 sdnb
100	0		\|a Luther Julia \|e Verfasser \|0 (DE-588)1031527095 \|4 aut
245	1	0	\|a Function of the transcription factor Fra1 in adipogenesis \|b = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese \|c von Julia Iris Luther
246	1	1	\|a Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese
264		1	\|c 2012
300			\|a 106 S. \|b graph. Darst.
336			\|b txt \|2 rdacontent
337			\|b n \|2 rdamedia
338			\|b nc \|2 rdacarrier
502			\|a Erlangen-Nürnberg, Univ., Diss., 2012
650	0	7	\|a Fettgewebe \|0 (DE-588)4154224-1 \|2 gnd \|9 rswk-swf
650	0	7	\|a Transkriptionsfaktor \|0 (DE-588)4303350-7 \|2 gnd \|9 rswk-swf
650	0	7	\|a Fettsucht \|0 (DE-588)4016953-4 \|2 gnd \|9 rswk-swf
655		7	\|0 (DE-588)4113937-9 \|a Hochschulschrift \|2 gnd-content
689	0	0	\|a Fettgewebe \|0 (DE-588)4154224-1 \|D s
689	0	1	\|a Transkriptionsfaktor \|0 (DE-588)4303350-7 \|D s
689	0		\|5 DE-604
689	1	0	\|a Fettsucht \|0 (DE-588)4016953-4 \|D s
689	1	1	\|a Transkriptionsfaktor \|0 (DE-588)4303350-7 \|D s
689	1		\|5 DE-604
776	0	8	\|i Erscheint auch als \|n Online-Ausgabe \|o urn:nbn:de:bvb:29-opus-40161
856	4		\|u https://open.fau.de/handle/openfau/2798 \|x Verlag \|z kostenfrei \|3 Volltext
856	4		\|u http://d-nb.info/1029374260/34 \|x Langzeitarchivierung Nationalbibliothek
856	4		\|u https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-40161 \|x Resolving-System
856	4	2	\|m DNB Datenaustausch \|q application/pdf \|u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025745519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA \|3 Inhaltsverzeichnis
912			\|a ebook
943	1		\|a oai:aleph.bib-bvb.de:BVB01-025745519

Record in the Search Index

DE-BY-OTHR_katkey	5087725
DE-BY-TUM_katkey	1912393
_version_	1831256852344602625
adam_text	IMAGE 1 TABLE O F CONTENTS TABLE OF CONTENTS ABSTRACT 7 ZUSAMMENFASSUNG 8 1 INTRODUCTION 10 1.1 BONE 10 1.1.1 BONE TISSUE: COMPOSITION AND FUNCTION 10 1.1.2 BONE DEVELOPMENT 10 1.1.3 BONE CELLS AND BONE REMODELING 11 1.2 ADIPOSE TISSUE 12 1.2.1 BROWN AND WHITE ADIPOSE TISSUE 12 1.2.2 FACTORS PRODUCED BY ADIPOSE TISSUE: ADIPOKINES 13 1.2.3 THE CO-REGULATION O F ADIPOSE TISSUE AND BONE 14 1.3 MESENCHYMAL CELL DIFFERENTIATION 15 1.3.1 DIFFERENTIATION O F MESENCHYMAL STROMAL CELLS (MSC) IS 1.3.2 CHONDROCYTE DIFFERENTIATION 16 1.3.3 MYOBLAST AND BROWN ADIPOCYTE DIFFERENTIATION 17 1.3.4 TRANSCRIPTIONAL CONTROL O F ADIPOGENESIS 17 1.3.5 OSTEOBLAST DIFFERENTIATION 19 1.3.6 RECIPROCAL REGULATION O F OSTEOBLASTOGENESIS AND ADIPOGENESIS 20 1.4 AP-1 TRANSCRIPTION FACTOR 20 1.4.1 STRUCTURE AND COMPOSITION O F THE AP-1 TRANSCRIPTION FACTOR 21 1.4.2 REGULATION OF TRANSCRIPTIONAL ACTIVITY 21 1.4.3 FUNCTIONS O F AP-1 22 1.4.4 FUNCTIONS O F AP-1 IN BONE DEVELOPMENT 23 1.4.5 FUNCTIONS O F AP-1 IN ADIPOCYTE DEVELOPMENT 24 1.5 AIM OF THE WORK 24 2. MATERIALS AND METHODS 26 2.1 MATERIALS 26 2.1.1 BUFFER AND STAINING SOLUTIONS 26 2.1.2 CELL CULTURE MEDIA AND SUPPLEMENTS 29 2.1.3 OLIGONUCLEOTIDES 30 - 4 - HTTP://D-NB.INFO/1031268510 IMAGE 2 TABLE O F CONTENTS 2.1.4 ANTIBODIES 32 2.1.5 PLASMIDS 33 2.1.6 MOLECULAR WEIGHT MARKER AND LOADING DYE 33 2.1.7 REAGENTS FOR CELL CULTURE 33 2.1.8 KITS AND ENZYMES 34 2.1.9 CHEMICALS 36 2.1.10 CONSUMABLES 38 2.1.11 LABORATORY INSTRUMENTS 38 2.2 METHODS 40 2.2.1 ANIMAL EXPERIMENTS 40 2.2.2 CELL CULTURE 41 2.2.3 METHODS TO STUDY RNA 4 3 2.2.4 METHODS TO STUDY DNA 44 2.2.5 METHODS TO STUDY PROTEIN 45 2.2.6 SEQUENCE ANALYSIS 48 2.2.7 STATISTICAL ANALYSIS 48 3 RESULTS 50 3.1 NO CLEAR ADIPOGENIC PHENOTYPE O F AP2 -ERE F R A L ^ M I C E 50 3.2 LIPODYSTROPHY PHENOTYPE IN MICE OVEREXPRESSING FRAL 52 3.2.1 SEVERE LIPODYSTROPHY IN FRAL TRANSGENIC MICE 52 3.2.2 NORMAL BROWN ADIPOSE TISSUE (BAT) 54 3.2.3 IMMATURE WHITE ADIPOSE TISSUE (WAT) IN MICE OVEREXPRESSING FRAL 55 3.2.4 NORMAL FOOD INTAKE AND INSULIN LEVELS IN FRAL TRANSGENIC MICE 57 3.2.5 INCREASED INSULIN SENSITIVITY, SERUM TRIGLYCERIDE AND NON-ESTERIFIED FATTY ACID LEVELS IN FRAL TRANSGENIC MICE 58 3.2.6 NO ECTOPIC LIPID DEPOSITION IN LIVER OR MUSCLE O F FRAL TRANSGENIC MICE 60 3.2.7 DECREASED EXPRESSION OF GENES INVOLVED IN LIPOGENESIS AND LIPOLYSIS IN THE ADIPOSE TISSUE O F FRAL TG MICE 60 3.2.8 NO CORRECTION O F THE LIPODYSTROPHIC PHENOTYPE BY HIGH FAT DIET 63 3.3 REDUCED DIFFERENTIATION TO ADIPOCYTES IN THE WAT OF FRAL TRANSGENIC MICE 65 3.3.1 NO TRANSDIFFERENTIATION O F WAT TOWARD BAT IN FRAL TRANSGENIC MICE 65 3.3.2 EXPRESSION O F ADIPOKINES IN WHITE ADIPOSE TISSUE O F FRAL TRANSGENIC MICE 65 - 5 - IMAGE 3 TABLE O F CONTENTS 3.3.3 NO CHANGE IN THE EXPRESSION O F MARKERS FOR ADIPOCYTE PROGENITOR CELLS IN WHITE ADIPOSE TISSUE O F FRAL TRANSGENIC MICE 67 3.3.4 NO CHANGE IN AP-1 EXPRESSION IN THE WAT O F FRAL TRANSGENIC MICE 67 3.3.5 DECREASED C/EBPA EXPRESSION IN WHITE ADIPOSE TISSUE OVEREXPRESSING FRAL 68 3.4 IN VITRO ANALYSIS OF THE ROLE OF FRAL DURING ADIPOCYTE DIFFERENTIATION 71 3.4.1 CELL AUTONOMOUS DECREASED ADIPOGENESIS O F FRAL TRANSGENIC MESENCHYMAL CELLS 71 3.4.2 NORMAL RESPONSES O F FRAL TRANSGENIC MESENCHYMAL PROGENITORS TO ADIPOGENIC STIMULATION 72 3.4.3 FRAL OVEREXPRESSION DIRECTLY BLOCKS ADIPOGENESIS 74 3.4.4 NO DETECTABLE REGULATION O F C/EBPA EXPRESSION VIA BINDING O F FRAL TO C/EBPP 75 3.4.5 FRAL DIRECTLY INHIBITS THE TRANSCRIPTION O F C/EBPA 76 4. DISCUSSION 79 4.1 PHENOTYPE OF THE FRAL TRANSGENIC AND THE FRAL KNOCKOUT MOUSE 79 4.2 FRAL OVEREXPRESSION DECREASES ADIPOSE TISSUE MASS 80 4.3 FRAL REGULATES ADIPOCYTE DIFFERENTIATION IN A CELL AUTONOMOUS MANNER 81 4.4 FRAL DIRECTLY REGULATES ADIPOCYTE DIFFERENTIATION BY REDUCING C/EBPA EXPRESSION 82 4.5 INFLUENCE OF THE REDUCED AMOUNT OF MATURE ADIPOCYTES ON METABOLISM 84 4.6 PHENOTYPE OF MICE WITH AN ADIPOCYTE-SPECIFIC DEFICIENCY OF FRAL 86 4.7 CONCLUSIONS 86 5 REFERENCES 88 6 APPENDIX 100 6.1 ABBREVIATIONS 100 6.2 CLONED SEQUENCE OF THE CEJPAPROMOTER 102 6.3 ACKNOWLEDGEMENTS 104 6.4 PUBLICATIONS AND PRESENTATIONS 105 - 6 -
any_adam_object	1
author	Luther Julia
author_GND	(DE-588)1031527095
author_facet	Luther Julia
author_role	aut
author_sort	Luther Julia
author_variant	l j lj
building	Verbundindex
bvnumber	BV040767062
collection	ebook
ctrlnum	(OCoLC)828809888 (DE-599)DNB1029374260
dewey-full	610
dewey-hundreds	600 - Technology (Applied sciences)
dewey-ones	610 - Medicine and health
dewey-raw	610
dewey-search	610
dewey-sort	3610
dewey-tens	610 - Medicine and health
discipline	Medizin
format	Thesis Book
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02289nam a2200517zc 4500</leader><controlfield tag="001">BV040767062</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20150206 </controlfield><controlfield tag="007">t\|</controlfield><controlfield tag="008">130221s2012 gw d\|\|\| m\|\|\| 00\|\|\| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">13,O02</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1029374260</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)828809888</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1029374260</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-BY</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield><subfield code="a">DE-29T</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">610</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Luther Julia</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1031527095</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Function of the transcription factor Fra1 in adipogenesis</subfield><subfield code="b">= Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese</subfield><subfield code="c">von Julia Iris Luther</subfield></datafield><datafield tag="246" ind1="1" ind2="1"><subfield code="a">Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">106 S.</subfield><subfield code="b">graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Erlangen-Nürnberg, Univ., Diss., 2012</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Fettgewebe</subfield><subfield code="0">(DE-588)4154224-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Transkriptionsfaktor</subfield><subfield code="0">(DE-588)4303350-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Fettsucht</subfield><subfield code="0">(DE-588)4016953-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Fettgewebe</subfield><subfield code="0">(DE-588)4154224-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Transkriptionsfaktor</subfield><subfield code="0">(DE-588)4303350-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Fettsucht</subfield><subfield code="0">(DE-588)4016953-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Transkriptionsfaktor</subfield><subfield code="0">(DE-588)4303350-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:29-opus-40161</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://open.fau.de/handle/openfau/2798</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">http://d-nb.info/1029374260/34</subfield><subfield code="x">Langzeitarchivierung Nationalbibliothek</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-40161</subfield><subfield code="x">Resolving-System</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025745519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025745519</subfield></datafield></record></collection>
genre	(DE-588)4113937-9 Hochschulschrift gnd-content
genre_facet	Hochschulschrift
id	DE-604.BV040767062
illustrated	Illustrated
indexdate	2024-12-20T16:25:16Z
institution	BVB
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-025745519
oclc_num	828809888
open_access_boolean	1
owner	DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T
owner_facet	DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T
physical	106 S. graph. Darst.
psigel	ebook
publishDate	2012
publishDateSearch	2012
publishDateSort	2012
record_format	marc
spellingShingle	Luther Julia Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese Fettgewebe (DE-588)4154224-1 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Fettsucht (DE-588)4016953-4 gnd
subject_GND	(DE-588)4154224-1 (DE-588)4303350-7 (DE-588)4016953-4 (DE-588)4113937-9
title	Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese
title_alt	Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese
title_auth	Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese
title_exact_search	Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese
title_full	Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese von Julia Iris Luther
title_fullStr	Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese von Julia Iris Luther
title_full_unstemmed	Function of the transcription factor Fra1 in adipogenesis = Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese von Julia Iris Luther
title_short	Function of the transcription factor Fra1 in adipogenesis
title_sort	function of the transcription factor fra1 in adipogenesis die funktion des transkriptionsfaktors fra1 in der adipogenese
title_sub	= Die Funktion des Transkriptionsfaktors Fra1 in der Adipogenese
topic	Fettgewebe (DE-588)4154224-1 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Fettsucht (DE-588)4016953-4 gnd
topic_facet	Fettgewebe Transkriptionsfaktor Fettsucht Hochschulschrift
url	https://open.fau.de/handle/openfau/2798 http://d-nb.info/1029374260/34 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-40161 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025745519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT lutherjulia functionofthetranscriptionfactorfra1inadipogenesisdiefunktiondestranskriptionsfaktorsfra1inderadipogenese AT lutherjulia diefunktiondestranskriptionsfaktorsfra1inderadipogenese

Availability

Order (Login required)

Read online (freely available)
Table of Contents
Order paper/chapter scan