Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
|
| Schlagwörter: | |
| Links: | https://open.fau.de/handle/openfau/2364 http://d-nb.info/1026365406/34 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35245 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025484700&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 70 S. Ill. |
Internformat
MARC
| LEADER | 00000nam a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV040657889 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t| | ||
| 008 | 130108s2012 gw a||| m||| 00||| eng d | ||
| 015 | |a 12,O10 |2 dnb | ||
| 016 | 7 | |a 1026365406 |2 DE-101 | |
| 035 | |a (OCoLC)824731368 | ||
| 035 | |a (DE-599)DNB1026365406 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE-BY | ||
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 | ||
| 082 | 0 | |a 610 | |
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Yuan, Quan |d 1982- |e Verfasser |0 (DE-588)1026797438 |4 aut | |
| 245 | 1 | 0 | |a Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |c von Quan Yuan |
| 264 | 1 | |c 2012 | |
| 300 | |a 70 S. |b Ill. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2012 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:29-opus-35245 |
| 856 | 4 | |u https://open.fau.de/handle/openfau/2364 |x Verlag |z kostenfrei |3 Volltext | |
| 856 | 4 | |u http://d-nb.info/1026365406/34 |x Langzeitarchivierung Nationalbibliothek | |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35245 |x Resolving-System | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025484700&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-025484700 | |
Datensatz im Suchindex
| DE-BY-TUM_katkey | 1904417 |
|---|---|
| _version_ | 1821935085996212224 |
| adam_text | IMAGE 1
TABLE OF CONTENTS
ABSTRACT 1
ZUSAMMENSFASSUNG 3
1. INTRODUCTION: 5
1.1. TISSUE ENGINEERING 5
1.1.1. THE CHALLENGING: VASCULARIZATION 6
1.1.2. STRATEGIES FOR VASCULARIZATION: 7
1.2. AV LOOP MODEL 8
1.2.1. DEVELOPMENT OF AV LOOP 8
1.2.2. MATRIX IN AV LOOP MODEL: 9
1.2.3. INVESTIGATE METHODS FOR AV LOOPS: 11
1.3. HYPOXIA, HIF-1A AND VASCULOGENESIS 11
1.3.1. STRUCTURE 12
1.3.2. KINETIC OF INDUCTION AND DEGRADATION: 13
1.3.3. DEGRADATION BY HYDROXYLATION 13
1.3.4. ANGIOGENIC TARGET GENES AND MOLECULES 15
2. MATERIALS AND METHODS 17
2.1. RAT: 17
2.2. ISOLATION CHAMBER: 17
2.3. LIQUID REAGENT PREPARATION: 18
2.3.1. FIBRIN AND THROMBIN: 18
2.3.2. 4%PARAFORMALDEHYDE (PFA): 18
2.3.3. WASHING BUFFER 18
2.3.4. FAST RED CHROMOGEN 19
2.4. ANIMAL EXPERIMENTS 19
2.4.1. GROUP DESIGNS: 19
HTTP://D-NB.INFO/1026826136
IMAGE 2
2.4.2. PREOPERATIVE TREATMENT: 19
2.4.3. LOOP PREPARATION: 19
2.4.4. CHAMBER IMPLANTATION: 20
2.4.5. POSTOPERATIVE TREATMENT: 21
2.4.6. EXPLANTATION: 23
2.5. HISTOLOGY: 23
2.5.1. GENERAL PREPARATION: 23
2.5.2. IMMUNOHISTOCHEMISTRY (IHC) 25
2.5.3. GENERAL POST IHC PROCESSING 28
2.6. REGIONS OF INTEREST (ROI) DEFINITION IN THREE DIMENSIONS 29
2.6.1. REGIONS OF INTEREST (ROI) 29
2.6.2. COUNTING: 31
3. RESULTS: 32
3.1. HIF-1A POSITIVE AND GENERAL CELLS DISTRIBUTION 32
3.1.1. INTERACTION: DAY*DISTANCE: 33
3.2. HIF-1A AND PIMONIDAZOLE STAINING IN CONSECUTIVE SLIDES 36
3.3. ED1, HIF-1A CO LOCALIZATION 38
3.4. HIF-1A AND LECTIN 40
4. DISCUSSION: 43
4.1. THE PATTERN OF HYPOXIA WITHIN AV LOOP 44
4.1.1. THE HIF-1A POSITIVE RATE 44
4.1.2. CELL DISTRIBUTION 45
4.1.3. BS-I LECTIN VS LECTIN-HIF1A IHC 45
4.1.4. PIMONIDAZOLE VS HIF-1A 46
4.2. THE PATTERN OF VASCULARIZATION IN THE AV LOOP: 47
4.2.1. WHEN? 47
4.2.2. WHERE? 48
IMAGE 3
4.2.3. HOW? 48
4.2.4. WHY? 49
4.3. THE MODEL PREPARATION: WHAT IS CRUCIAL TO GAIN A PATENT AV LOOP? 50
4.4. CONCLUSION AND PERSPECTIVE 53
4.4.1. CONCLUSION 53
4.4.2. APPLICATION OF THE AV LOOP: 54
4.4.3. REGARDING THE ASSESSMENT FOR THE MODEL: WHERE TO PICK UP THE ROI
IN THE AV LOOP? 55
4.4.4. LIMITATION OF THE STUDY: 55
4.4.5. FURTHER PLAN: THE APPLICATION OF PHD INHIBITION 56
ACKNOWLEDGEMENT: 67
CURRICULUM VITAE 69
|
| any_adam_object | 1 |
| author | Yuan, Quan 1982- |
| author_GND | (DE-588)1026797438 |
| author_facet | Yuan, Quan 1982- |
| author_role | aut |
| author_sort | Yuan, Quan 1982- |
| author_variant | q y qy |
| building | Verbundindex |
| bvnumber | BV040657889 |
| collection | ebook |
| ctrlnum | (OCoLC)824731368 (DE-599)DNB1026365406 |
| dewey-full | 610 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610 |
| dewey-search | 610 |
| dewey-sort | 3610 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01757nam a2200397zc 4500</leader><controlfield tag="001">BV040657889</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">130108s2012 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">12,O10</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1026365406</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)824731368</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1026365406</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-BY</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">610</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Yuan, Quan</subfield><subfield code="d">1982-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1026797438</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model</subfield><subfield code="c">von Quan Yuan</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">70 S.</subfield><subfield code="b">Ill.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Erlangen-Nürnberg, Univ., Diss., 2012</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:29-opus-35245</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://open.fau.de/handle/openfau/2364</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">http://d-nb.info/1026365406/34</subfield><subfield code="x">Langzeitarchivierung Nationalbibliothek</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35245</subfield><subfield code="x">Resolving-System</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025484700&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025484700</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040657889 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T16:19:53Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025484700 |
| oclc_num | 824731368 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| physical | 70 S. Ill. |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spellingShingle | Yuan, Quan 1982- Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| subject_GND | (DE-588)4113937-9 |
| title | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| title_auth | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| title_exact_search | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| title_full | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model von Quan Yuan |
| title_fullStr | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model von Quan Yuan |
| title_full_unstemmed | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model von Quan Yuan |
| title_short | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| title_sort | quantification of hypoxia by hypoxia inducible factor 1alpha and its role in vascularization of the arterio venous loop in a rat isolation chamber model |
| topic_facet | Hochschulschrift |
| url | https://open.fau.de/handle/openfau/2364 http://d-nb.info/1026365406/34 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35245 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025484700&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT yuanquan quantificationofhypoxiabyhypoxiainduciblefactor1alphaanditsroleinvascularizationofthearteriovenousloopinaratisolationchambermodel |