In vitro culture studies of tick cell lines: "Endosymbionts in tick cell culture and evaluation of media conditions"
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
|
| Schlagwörter: | |
| Links: | http://edoc.ub.uni-muenchen.de/14663/ https://nbn-resolving.org/urn:nbn:de:bvb:19-146638 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025352965&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | 110 S. Ill. |
Internformat
MARC
| LEADER | 00000nam a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV040506339 | ||
| 003 | DE-604 | ||
| 005 | 20130123 | ||
| 007 | t| | ||
| 008 | 121026s2012 gw a||| m||| 00||| eng d | ||
| 016 | 7 | |a 1025047206 |2 DE-101 | |
| 035 | |a (OCoLC)816333289 | ||
| 035 | |a (DE-599)DNB1025047206 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE-BY | ||
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 | ||
| 084 | |a 630 |2 sdnb | ||
| 100 | 1 | |a Najm, Nour Addeen |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a In vitro culture studies of tick cell lines |b "Endosymbionts in tick cell culture and evaluation of media conditions" |c von Nour Addeen Najm |
| 264 | 1 | |c 2012 | |
| 300 | |a 110 S. |b Ill. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Ludwig-Maximilians-Universität, Diss., 2012 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:19-146638 |
| 856 | 4 | |u http://edoc.ub.uni-muenchen.de/14663/ |x Verlag |z kostenfrei | |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:19-146638 |x Resolving-System |z kostenfrei | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025352965&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-025352965 | |
Datensatz im Suchindex
| DE-BY-TUM_katkey | 1889627 |
|---|---|
| _version_ | 1821934561326530560 |
| adam_text | IMAGE 1
TABLE O F CONTENTS
ABBREVIATIONS 5
1. INTRODUCTION AND OBJECTIVES 7
2. LITERATURE REVIEW 9
2.1. TICKS 9
2.1.1. TAXONOMY O F TICKS 9
2.1.2. IXODID TICKS 10
2.1.2.1. LIFE CYCLE 10
2.1.2.1.1. THREE-HOST LIFE CYCLE 12
2.1.2.1.2. ONE AND TWO-HOST LIFE CYCLE 12
2.1.3. VETERINARY IMPORTANCE O F TICKS 13
2.1.4. VETERINARY IMPORTANCE O F TICK-BORNE DISEASES 13
2.2. TICK CELL LINES 14
2.2.1. CHARACTERISTICS 14
2.2.1.1. KARYOLOGY 14
2.2.1.2. GROWTH RATE 15
2.2.1.3. CELL TYPES 16
2.2.1.4. DEVELOPMENTAL PATTERNS 16
2.2.2. GROWTH REQUIREMENTS 17
2.2.2.1. BASAL MEDIA 17
2.2.2.2. SUPPLEMENTS 20
2.2.2.3. PHYSICAL AND CHEMICAL ENVIRONMENT 21
2.2.3. STORAGE O F TICK CELL LINES 22
2.2.4. CURRENT TREND 23
2.2.5. IMPORTANCE O F TICK CELL CULTURES 25
2.2.5.1. ISOLATION AND PROPAGATION O F PATHOGENS 25
2.2.5.1.1. VIROLOGY 25
1
HTTP://D-NB.INFO/1025574605
IMAGE 2
TABLE O F CONTENTS
2.2.5.1.2. PARASITOLOGY 26
2.2.5.1.3. BACTERIOLOGY 26
2.2.5.2. PATHOGENS GENOMICS AND PROTEOMICS 28
2.2.5.3. BIOLOGY O F TICKS AND PATHOGENS 28
2.3. SYMBIONTS 30
2.3.1. SYMBIONTS IN TICKS 30
2.3.2. SYMBIONTS IN TICK CELL CULTURES 31
2.3.3. CANDIDATUS MIDICHLORIA MITOCHONDRII 31
2.3.3.1. HISTORICAL OVERVIEW 32
2.3.3.2. CA. MIDICHLORIA MITOCHONDRII CHARACTERIZATION 33
2.3.3.3. DISTRIBUTION IN TICKS 33
2.4. POLYMERASE CHAIN REACTION (PCR) 34
2.4.1. PRINCIPLE O F PCR 34
2.4.2. REAL-TIME PCR 35
3. MATERIALS AND METHODS 38
3.1. GENERAL MATERIALS AND METHODS 38
3.1.1. TICK CELL LINES 38
3.1.2. CULTURE MEDIA 39
3.1.3. CULTIVATION CONDITIONS 39
3.2. EXPERIMENT 1: DETECTION OF ENDOSYMBIONTS IN TICK CELL LINES 40
3.2.1. DNA EXTRACTION 40
3.2.2. QUALITY AND QUANTITY O F EXTRACTED DNA 40
3.2.3. POLYMERASE CHAIN REACTION (PCR) 41
3.2.3.1. CONVENTIONAL PCR FOR DETECTION O F THE 16S RRNA GENE O F CA.
MIDICHLORIA
MITOCHONDRII AND RELATED BACTERIA 41
2
IMAGE 3
TABLE O F CONTENTS
3.2.3.2. QUANTITATIVE REAL-TIME PCR (QRT-PCR) FOR DETECTION O F THE GYRB
GENE CA.
MIDICHLORIA MITOCHONDRII 42
3.2.4. AGAROSE GEL ELECTROPHORESIS 44
3.2.5. DNA PURIFICATION 44
3.2.6. SEQUENCING AND SEQUENCE- PHYLOGENETIC ANALYSIS 44
3.3. EXPERIMENT 2: EVALUATION OF MEDIUM CONDITIONS FOR MAINTENANCE O F
TICK CELL LINES
45
3.3.1. EXPERIMENTAL CONDITIONS 45
3.3.2. MONITORING O F CULTURES 45
3.3.2.1. CELL MORPHOLOGY 45
3.3.2.2. CELL VIABILITY 46
3.3.3. STATISTICAL ANALYSIS 47
4. RESULTS 48
4.1. EXPERIMENT 1: DETECTION OF ENDOSYMBIONTS IN TICK CELL LINES 48
4.1.1. PUBLICATION 49
4.2. EXPERIMENT 2: EVALUATION OF MEDIUM CONDITIONS FOR MAINTENANCE O F
TICK CELL LINES
64
4.2.1. IDE8 CELL LINE 64
4.2.2. IRE/CTVM 19 CELL LINE 66
4.2.3. BME/CTVM 6 CELL LINE 67
4.2.4. BDE/CTVM 14 CELL LINE 69
5. DISCUSSION 71
5.1. DETECTION OF CA. MIDICHLORIA MITOCHONDRII IN TICK CELLS 71
5.2. EVALUATION OF DIFFERENT MEDIUM CONDITIONS 74
6. CONCLUSIONS 76
7. RECOMMENDATIONS 77
3
IMAGE 4
TABLE O F CONTENTS
7. SUMMARY 78
8. ZUSAMMENFASSUNG 80
9. REFERENCES 82
10. TABLES 96
11. FIGURES 97
12. APPENDIX 98
12.1. VIABILITY RATES AS THE MEAN O F REPLICATES FOR EACH CELL LINE AND
EACH CONDITION 98
12.2. STATISTICAL ANALYSIS 102
12.3. SEQUENCING DATA 106
12.3.1. 16S RRNA SEQUENCE COMPARISON O F CA. MIDICHLORIA MITOCHONDRII
106
12.3.2. PHYLOGENETIC ANALYSIS SEQUENCES 107
13. ACKNOWLEDGEMENTS 109
4
|
| any_adam_object | 1 |
| author | Najm, Nour Addeen |
| author_facet | Najm, Nour Addeen |
| author_role | aut |
| author_sort | Najm, Nour Addeen |
| author_variant | n a n na nan |
| building | Verbundindex |
| bvnumber | BV040506339 |
| collection | ebook |
| ctrlnum | (OCoLC)816333289 (DE-599)DNB1025047206 |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01581nam a2200361zc 4500</leader><controlfield tag="001">BV040506339</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20130123 </controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">121026s2012 gw a||| m||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1025047206</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)816333289</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1025047206</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-BY</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">630</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Najm, Nour Addeen</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">In vitro culture studies of tick cell lines</subfield><subfield code="b">"Endosymbionts in tick cell culture and evaluation of media conditions"</subfield><subfield code="c">von Nour Addeen Najm</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">110 S.</subfield><subfield code="b">Ill.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">München, Ludwig-Maximilians-Universität, Diss., 2012</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:19-146638</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">http://edoc.ub.uni-muenchen.de/14663/</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:19-146638</subfield><subfield code="x">Resolving-System</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025352965&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025352965</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040506339 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T16:16:25Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025352965 |
| oclc_num | 816333289 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
| physical | 110 S. Ill. |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spellingShingle | Najm, Nour Addeen In vitro culture studies of tick cell lines "Endosymbionts in tick cell culture and evaluation of media conditions" |
| subject_GND | (DE-588)4113937-9 |
| title | In vitro culture studies of tick cell lines "Endosymbionts in tick cell culture and evaluation of media conditions" |
| title_auth | In vitro culture studies of tick cell lines "Endosymbionts in tick cell culture and evaluation of media conditions" |
| title_exact_search | In vitro culture studies of tick cell lines "Endosymbionts in tick cell culture and evaluation of media conditions" |
| title_full | In vitro culture studies of tick cell lines "Endosymbionts in tick cell culture and evaluation of media conditions" von Nour Addeen Najm |
| title_fullStr | In vitro culture studies of tick cell lines "Endosymbionts in tick cell culture and evaluation of media conditions" von Nour Addeen Najm |
| title_full_unstemmed | In vitro culture studies of tick cell lines "Endosymbionts in tick cell culture and evaluation of media conditions" von Nour Addeen Najm |
| title_short | In vitro culture studies of tick cell lines |
| title_sort | in vitro culture studies of tick cell lines endosymbionts in tick cell culture and evaluation of media conditions |
| title_sub | "Endosymbionts in tick cell culture and evaluation of media conditions" |
| topic_facet | Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/14663/ https://nbn-resolving.org/urn:nbn:de:bvb:19-146638 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025352965&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT najmnouraddeen invitroculturestudiesoftickcelllinesendosymbiontsintickcellcultureandevaluationofmediaconditions |