Verfügbarkeit: Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer

Gespeichert in:

Bibliographische Detailangaben
Beteilige Person:	Richter, Anne (VerfasserIn)
Format:	Hochschulschrift/Dissertation Buch
Sprache:	Englisch
Veröffentlicht:	2012
Schlagwörter:	Hochschulschrift
Links:	http://edoc.ub.uni-muenchen.de/14603/ http://d-nb.info/1024658651/34 https://nbn-resolving.org/urn:nbn:de:bvb:19-146039 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025273192&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Umfang:	109 S. graph. Darst.

Internformat

MARC


LEADER	00000nam a2200000zc 4500
001	BV040420439
003	DE-604
005	20121107
007	t\|
008	120917s2012 gw d\|\|\| m\|\|\| 00\|\|\| eng d
015			\|a 12,O08 \|2 dnb
016	7		\|a 1024658651 \|2 DE-101
035			\|a (OCoLC)815895539
035			\|a (DE-599)DNB1024658651
040			\|a DE-604 \|b ger \|e rakddb
041	0		\|a eng
044			\|a gw \|c XA-DE-BY
049			\|a DE-384 \|a DE-473 \|a DE-703 \|a DE-1051 \|a DE-824 \|a DE-29 \|a DE-12 \|a DE-91 \|a DE-19 \|a DE-1049 \|a DE-92 \|a DE-739 \|a DE-898 \|a DE-355 \|a DE-706 \|a DE-20 \|a DE-1102 \|a DE-1029
084			\|a 630 \|2 sdnb
100	1		\|a Richter, Anne \|e Verfasser \|4 aut
245	1	0	\|a Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer \|c Anne Richter
264		1	\|c 2012
300			\|a 109 S. \|b graph. Darst.
336			\|b txt \|2 rdacontent
337			\|b n \|2 rdamedia
338			\|b nc \|2 rdacarrier
502			\|a München, Univ., Diss., 2012
655		7	\|0 (DE-588)4113937-9 \|a Hochschulschrift \|2 gnd-content
776	0	8	\|i Erscheint auch als \|n Online-Ausgabe \|o urn:nbn:de:bvb:19-146039
856	4	1	\|u http://edoc.ub.uni-muenchen.de/14603/ \|x Verlag \|z kostenfrei \|3 Volltext
856	4		\|u http://d-nb.info/1024658651/34 \|x Langzeitarchivierung Nationalbibliothek
856	4		\|u https://nbn-resolving.org/urn:nbn:de:bvb:19-146039 \|x Resolving-System
856	4	2	\|m DNB Datenaustausch \|q application/pdf \|u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025273192&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA \|3 Inhaltsverzeichnis
912			\|a ebook
943	1		\|a oai:aleph.bib-bvb.de:BVB01-025273192

Datensatz im Suchindex

DE-BY-OTHR_katkey	5017488
DE-BY-TUM_katkey	1874222
DE-BY-UBR_katkey	5017488
_version_	1835108005153079296
adam_text	IMAGE 1 TABLE O F CONTENTS 1 INTRODUCTION 2 REVIEW OF THE LITERATURE - 3 - 2.1 PIG MODELS FOR BIOMEDICAL RESEARCH - 3 - 2.2 GENETIC ENGINEERING OF PIGS - 5 - 2.2.1 PRONUCLEAR DNA MICROINJECTION - 5 - 2.2.2 SPERM-MEDIATED GENE TRANSFER - 6 - 2.2.3 VIRAL TRANSGENESIS - 6 - 2.2.4 SOMATIC CELL NUCLEAR TRANSFER (SCNT) - 7 - 2.2.4.1 OVERVIEW - 7 - 2.2.4.2 CLONING EFFICIENCY - 8 - 2.2.4.3 FACTORS INFLUENCING THE EFFICIENCY O F SCNT - 9 - 2.3 SUITABLE CELL TYPES FOR NUCLEAR TRANSFER IN PIGS - 1 0 - 2.3.1 PLURIPOTENT STEM CELLS - 1 0 - 2.3.2 SOMATIC CELLS -11 - 2.4 GENETIC MODIFICATIONS - 1 3 - 2.4.1 ADDITIVE GENE TRANSFER - 13 - 2.4.2 GENE TARGETING - 15 - 2.4.2.1 HR USING CONVENTIONAL VECTORS AND BAC-TECHNOLOGY - 1 5 - 2.4.2.2 DESIGNED NUCLEASES - 1 6 - 2.4.3 SELECTION SYSTEMS - 1 8 - 2.5 NON-VIRAL GENE TRANSFER - 1 9 - 2.5.1. CHEMICAL TRANSFECTION - 1 9 - 2.5.2 PHYSICAL TRANSFECTION METHODS - 2 0 - 3 CELLS, MATERIAL AND METHODS - 2 3 - 3.1 MATERIAL - 2 3 - 3.1.1 APPARATUSES - 2 3 - 3.1.2 CHEMICALS - 2 4 - 3.1.3 CELL CULTURE - 2 4 - HTTP://D-NB.INFO/1025134036 IMAGE 2 3.1.4 CONSUMPTION AND OTHER WORKING MATERIAL - 25 - 3.1.5 KITS - 2 6 - 3.1.6 ANTIBIOTICS AND ANTIMYCOTICS - 26 - 3.1.7 CULTURE MEDIA AND SUPPLEMENTS FOR CELL CULTURE - 26 - 3.1.8 PLASMID - 2 9 - 3.1.9 BACTERIAL STRAIN - 29 - 3.1.10 SOFTWARE - 2 9 - 3.2 METHODS - 29 - 3.2.1 CELL CULTURE - 2 9 - 3.2.1.1 ISOLATION O F PRIMARY CELLS (PKCM, PKC2109, PEF0110 AND PFF26) - 29 - 3.2.1.2 SUBCULTURE O F CELLS - 3 1 - 3.2.1.3 COUNTING O F CELLS - 3 2 - 3.2.1.4 CRYOPRESERVATION O F CELLS - 3 2 - 3.2.1.5 THAWING O F CELLS - 3 2 - 3.2.1.6 CHROMOSOME PREPARATION - 3 2 - 3.2.1.7 MTT-BASED CELL PROLIFERATION ASSAY - 3 3 - 3.2.1.8 GROWTH CURVE AND POPULATION DOUBLING TIME - 3 4 - 3.2.1.9 TRANSFECTION O F CELLS - 34 - 3.2.1.9.1 CHEMICAL TRANSFECTION - 3 4 - 3.2.1.9.2 PHYSICAL TRANSFECTION - 3 5 - 3.2.1.10 DETECTION O F APPROPRIATE ANTIBIOTIC CONCENTRATION FOR SELECTION - 36 - 3.2.1.11 SOMATIC CELL NUCLEAR TRANSFER AND EMBRYO TRANSFER - 3 6 - 3.2.1.12 STATISTICAL ANALYZES - 3 6 - 3.2.2 MOLECULAR BIOLOGY METHODS - 3 7 - 3.2.2.1 HEAT SHOCK TRANSFORMATION - 37 - 3.2.2.2 ENDOTOXIN FREE ISOLATION O F PMAXGFP* DNA - 3 7 - 4 RESULTS - 38 - 4.1 EVALUATION OF SUITABLE CELL SOURCES FOR SCNT TO GENERATE TRANSGENIC PIGS - 38 - 4.1.1 CHARACTERIZATION AND COMPARISON O F PKC, PFF AND PEF CELL CULTURES - 38 - 4.1.1.1 MORPHOLOGY O F CELLS - 3 8 - 4.1.1.2 GROWTH POTENTIAL - 4 2 - 4.1.1.3 CHROMOSOME NUMBER - 4 5 - 4.1.1.4 SOMATIC CELL NUCLEAR TRANSFER - IN VITRO DEVELOPMENT COMPETENCE O F CLONED EMBRYOS - 4 6 - IMAGE 3 4.1.2 TRANSFECTION O F PORCINE KIDNEY CELLS - COMPARISON O F DIFFERENT METHODS - 48 - 4.1.2.1 CHEMICAL TRANSFECTION BY NANOFECTION AND LIPOFECTION - 49 - 4.1.2.2 PHYSICAL TRANSFECTION BY ELECTROPORATION AND NUCLEOFECTION - 52 - 4.2 GENERATION O F TRANSGENIC ANIMALS USING PKC, PFF AND PEF CELLS AS DONORS FOR SCNT - 56 - 4.2.1 ADDITIVE GENE TRANSFER, CLONING AND RECLONING USING DIFFERENT CELL POPULATIONS - 56 - 4.2.2 GENE TARGETING - 59 - 5 DISCUSSION - 62 - 5.1 MORPHOLOGY AND PROLIFERATION O F PRIMARY CELL CULTURES - 62 - 5.2 PROMOTING O F PROLIFERATION CAPACITY USING VARIOUS COATINGS - 65 - 5.3 COMPARISON O F NON-VIRAL GENE TRANSFER EFFICIENCIES - 66 - 5.4 EFFICIENCY O F / N VITRO/IN VIVO SCNT - 70 - 5.4.1 EFFECTS O F DONOR CELL SOURCE ON IN VITRO DEVELOPMENT O F SCNT EMBRYOS - 70 - 5.4.2 AN OVERVIEW O F CLONED TRANSGENIC PIGLETS ORIGINATING FROM DIFFERENT DONOR CELLS - 73 - 5.5 OUTLOOK - 74 - 6 SUMMARY - 76 - 2 ZUSAMMENFASSUNG - 78 - 8 INDEX OF FIGURES - 80 - 9 INDEX OF TABLES - 81 - 10 REFERENCES - 82 - 11 ACKNOWLEDGMENT - 1 0 9 -
any_adam_object	1
author	Richter, Anne
author_facet	Richter, Anne
author_role	aut
author_sort	Richter, Anne
author_variant	a r ar
building	Verbundindex
bvnumber	BV040420439
collection	ebook
ctrlnum	(OCoLC)815895539 (DE-599)DNB1024658651
discipline	Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau
format	Thesis Book
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01676nam a2200385zc 4500</leader><controlfield tag="001">BV040420439</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20121107 </controlfield><controlfield tag="007">t\|</controlfield><controlfield tag="008">120917s2012 gw d\|\|\| m\|\|\| 00\|\|\| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">12,O08</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1024658651</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)815895539</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1024658651</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-BY</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-384</subfield><subfield code="a">DE-473</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-1051</subfield><subfield code="a">DE-824</subfield><subfield code="a">DE-29</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-1049</subfield><subfield code="a">DE-92</subfield><subfield code="a">DE-739</subfield><subfield code="a">DE-898</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-706</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-1102</subfield><subfield code="a">DE-1029</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">630</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Richter, Anne</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer</subfield><subfield code="c">Anne Richter</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">109 S.</subfield><subfield code="b">graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">München, Univ., Diss., 2012</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:bvb:19-146039</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://edoc.ub.uni-muenchen.de/14603/</subfield><subfield code="x">Verlag</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">http://d-nb.info/1024658651/34</subfield><subfield code="x">Langzeitarchivierung Nationalbibliothek</subfield></datafield><datafield tag="856" ind1="4" ind2=" "><subfield code="u">https://nbn-resolving.org/urn:nbn:de:bvb:19-146039</subfield><subfield code="x">Resolving-System</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025273192&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025273192</subfield></datafield></record></collection>
genre	(DE-588)4113937-9 Hochschulschrift gnd-content
genre_facet	Hochschulschrift
id	DE-604.BV040420439
illustrated	Illustrated
indexdate	2024-12-20T16:14:44Z
institution	BVB
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-025273192
oclc_num	815895539
open_access_boolean	1
owner	DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-1029
owner_facet	DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-1029
physical	109 S. graph. Darst.
psigel	ebook
publishDate	2012
publishDateSearch	2012
publishDateSort	2012
record_format	marc
spellingShingle	Richter, Anne Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer
subject_GND	(DE-588)4113937-9
title	Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer
title_auth	Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer
title_exact_search	Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer
title_full	Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer Anne Richter
title_fullStr	Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer Anne Richter
title_full_unstemmed	Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer Anne Richter
title_short	Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer
title_sort	genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer
topic_facet	Hochschulschrift
url	http://edoc.ub.uni-muenchen.de/14603/ http://d-nb.info/1024658651/34 https://nbn-resolving.org/urn:nbn:de:bvb:19-146039 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025273192&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT richteranne geneticmodificationofprimarycellsforthegenerationoftransgenicpigmodelsusingsomaticcellnucleartransfer

Verfügbarkeit

Bestellen (Login erforderlich)

Online lesen (frei zugänglich)
Inhaltsverzeichnis
Paper/Kapitel scannen lassen