Availability: Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus

Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus:

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Bibliographic Details
Main Author:	Heinrich, Stefanie 1981- (Author)
Format:	Thesis/Dissertation Book
Language:	English
Published:	2011
Subjects:	Staphylococcus carnosus Penicillinbindungsprotein Hochschulschrift
Links:	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025216693&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Physical Description:	XII, 133 S. Ill., graph. Darst.

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MARC


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Record in the Search Index

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adam_text	IMAGE 1 INDEX INDEX OF CONTENT I INTRODUCTION 1 1.1 STAPHYLOCOCCI . 1 1.1.1 STAPHYLOCOCCUS AUREUS ABC 1 1.1.2 STAPHYLOCOCCUS CAMOSUS ABC 2 1.2 PEPTIDOGLYCAN ( MUREIN) 3 1.2.1 STRUCTURE 3 1.2.2 BIOSYNTHESIS 5 1.2.3 INTEGRATION OF NEW PEPTIDOGLYCAN 11 1.2.4 DEGRADATION OF PEPTIDOGLYCAN 15 1.3 PENICILLIN BINDING PROTEINS 18 1.3.1 S. AUREUS PBPS 21 1.3.2 PBPS OF S. CARNOSUS 23 1.4 SS-LACTAM ANTIBIOTICS 24 1.4.1 STRUCTURE AND FUNCTION 25 1.5 SS-LACTAM RESISTANCE 25 I.5.1 SS LACTAMASES 26 1.6 AIM OF THIS WORK 29 II MATERIAL AND METHODS 30 11.1 MATERIAL 30 II.1.1 CHEMICALS 30 11.1.2 INSTRUMENTS 32 11.1.3 OTHER MATERIALS 34 11.1.4 SOFTWARE 34 11.1.5 BIOMOLECULAR KITS 35 11.1.6 ENZYMES 36 11.1.7 DNA/PROTEIN ELECTROPHORESIS 36 11.1.8 ANTIBIOTICS 37 I HTTP://D-NB.INFO/1024827313 IMAGE 2 INDEX 11.1.9 ANTIBODIES 37 11.1.10 BACTERIAL STRAINS 38 11.1.11 SYNTHETIC OLIGONUCLEOTIDES FOR PCR CLONING 39 11.1.12 SYNTHETIC OLIGONUCLEOTIDES FOR NORTHERN BLOTTING 41 11.1.13 SYNTHETIC OLIGONUCLEOTIDES FOR DNA SEQUENCING 41 11.1.14 PLASMIDS 42 11.1.15 VECTOR MAPS 43 11.2 METHODS 44 11.2.1 MICROBIAL METHODS 44 11.2.2 METHODS OF MOLECULAR BIOLOGY 46 11.2.3 METHODS FOR WORKING WITH PROTEINS 55 11.2.4 METHODS FOR FINDING PROTEIN-PROTEIN INTERACTIONS 68 11.3 METHODS FOR THE CHARACTERIZATION OF APBP3 71 11.3.1 E-TEST (EPSILOMETER TEST) 71 11.3.2 MIC-TEST 71 11.3.3 NORTHERN BLOT 72 III RESULTS 78 111.1 CLONING OF PBPS WITH N-TERMINAL HIS 6-TAG 78 111.2 CLONING AND OVEREXPRESSION OF PBPS WITH C-TERMINAL HIS 6-TAG 80 111.2.1 OVEREXPRESSION AND PURIFICATION OF PBP1 82 111.2.2 OVEREXPRESSION AND PURIFICATION OF PBP2 85 111.2.3 OVEREXPRESSION AND PURIFICATION OF PBP3 90 111.2.4 CLONING AND OVEREXPRESSION OF PBP4 92 111.2.5 CLONING OF PBP 4 WITH GST-TAG 96 111.2.6 BINDING ASSAYS WITH THE PBPS 97 111.2.7 PURIFICATION OF THE ANTIBODIES 98 111.2.8 TESTING OF THE ANTIBODIES 99 111.2.9 IMMUNOPRECIPITATION 100 111.2.10 CROSS-IINKING 104 111.2.11 CHARACTERIZATION OF A PBP3 108 II IMAGE 3 INDEX IV DISCUSSION 113 IV.1 OVEREXPRESSION AND PURIFICATION OF THE PBPS FROM S. CARNOSUS 113 IV.2 ACTIVITY TESTS 115 IV.3 INTERACTION STUDIES 116 V CONCLUSION AND OUTLOOK 119 VI REFERENCES 120 VII APPENDIX 129 VII.1.1 SEQUENCE OF P B P L (SCA_0794) ENCODING FOR PBP1 OF S.CARNOSUS TM300 129 VII.1.2 SEQUENCE OF PBP2 (SCA_1084) ENCODING FOR PBP2 OF S.CARNOSUS TM300 130 VII.1.3 SEQUENCE OF PBP3 (SCA_1173) ENCODING FOR PBP3 OF S.CARNOSUS TM300 131 VII.1.4 SEQUENCE OF PBP4 (SCA_0291) ENCODING FOR PBP4 OF S.CARNOSUS TM300 132 III
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language	English
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spellingShingle	Heinrich, Stefanie 1981- Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus Staphylococcus carnosus (DE-588)4316001-3 gnd Penicillinbindungsprotein (DE-588)4255851-7 gnd
subject_GND	(DE-588)4316001-3 (DE-588)4255851-7 (DE-588)4113937-9
title	Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus
title_auth	Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus
title_exact_search	Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus
title_full	Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus vorgelegt von Stefanie Heinrich
title_fullStr	Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus vorgelegt von Stefanie Heinrich
title_full_unstemmed	Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus vorgelegt von Stefanie Heinrich
title_short	Purification and investigation of the penicillin binding proteins of Staphylococcus carnosus
title_sort	purification and investigation of the penicillin binding proteins of staphylococcus carnosus
topic	Staphylococcus carnosus (DE-588)4316001-3 gnd Penicillinbindungsprotein (DE-588)4255851-7 gnd
topic_facet	Staphylococcus carnosus Penicillinbindungsprotein Hochschulschrift
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025216693&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT heinrichstefanie purificationandinvestigationofthepenicillinbindingproteinsofstaphylococcuscarnosus

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