Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis/Dissertation Book |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017391755&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Physical Description: | 181 S. Ill., graph. Darst. 21 cm |
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| 245 | 1 | 0 | |a Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli |c von Patrick Müller |
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Record in the Search Index
| _version_ | 1819260210017992704 |
|---|---|
| adam_text | Titel: Interactions of essential cell division and cell elongation proteins with murein synthases in Escher
Autor: Müller, Patrick
Jahr: 2009
Table index
Tab. 1 Murein synthases in E. coli.
Tab. 2 Pipet scheme for SDS - gels.
Tab. 3 Pipet scheme for blue native gradient gels.
Index
I Introduction 1
1.1 Background 1
1.2 The bacterial cell envelope 1
1.2.1 Themurein 3
1.2.2 Biosynthesis of the murein sacculus 9
1.3 Mureinsynthases and hydrolases in E co// 18
1.3.1 Penicillin-binding proteins (PBPs) 18
1.3.2 Murein hydrolases 26
1.3.3 Penicillin-insensitive peptidoglycan synthesis 28
1.4 Cell elongation and division in E. coti 30
1.4.1 Hypothetical multienzyme complex involved in murein synthesis during 30
cell division and cell elongation
1.4.2 Thedivisome 34
1.4.3 Selection of the division site 46
1.5 The Mre proteins 49
1.6 Scientific objectives 56
II Material and methods 57
2.1 Material 57
2.1.1 Chemicals used in this study 57
2.1.2 Kits 59
2.1.3 Enzymes 59
2.1.4 Proteins 59
2.1.5 Antibodies ^
2.1.6 Antibiotics 61
2.1.7 Molecular weight markers 61
2.1.8 Other materials 61
2.1.9 Lab equipment te
2.1.10 E co//strains a
2.1.11 Plasmids w
2.1.12 Oligonucleotides for sequencing and PCR 64
2.2 Methods 65
2.2.1 Microbial methods 65
2.2.2 DNA methods 66
74
2.2.3 Protein methods
2.2.4 Other methods 104
III Results 106
3.1 Interaction of FtsN with the penicillin binding-protein 1B 1 °6
3.1.1 Pull-down experiments with 1,6-anhMP immobilized on AGIO 107
3.1.2 Verification of FtsN-PBP1 B interaction by BN-PAGE 113
11Q
3.2 Interaction of MreC with penicillin-binding proteins
118
3.2.1 Overproduction and purification of MreC-ht
3.2.2 In vitro interaction between MreC-ht and proteins involved in cell 123
elongation and division
3.2.3 In vivo cross-linking and co-IP of MreC and PBP2
3.2.4 Pull-down of MreC with isolated E. coli sacculi 129
IV Discussion 131
4.1 Interaction of FtsN with the penicillin binding-protein 1B 132
4.2 Interaction between PBP2, MreC and PBP1A 138
V Summary 142
VI Literature 143
Poster and publications 180
Curriculum vitae 181
|
| any_adam_object | 1 |
| author | Müller, Patrick 1978- |
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| ctrlnum | (OCoLC)318221370 (DE-599)DNB99285914X |
| dewey-full | 571.8429342 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.8429342 |
| dewey-search | 571.8429342 |
| dewey-sort | 3571.8429342 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV035472110 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T13:33:16Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017391755 |
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| physical | 181 S. Ill., graph. Darst. 21 cm |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| record_format | marc |
| spellingShingle | Müller, Patrick 1978- Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli |
| subject_GND | (DE-588)4113937-9 |
| title | Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli |
| title_auth | Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli |
| title_exact_search | Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli |
| title_full | Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli von Patrick Müller |
| title_fullStr | Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli von Patrick Müller |
| title_full_unstemmed | Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli von Patrick Müller |
| title_short | Interactions of essential cell division and cell elongation proteins with murein synthases in Escherichia coli |
| title_sort | interactions of essential cell division and cell elongation proteins with murein synthases in escherichia coli |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017391755&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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